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Q42624 (GLNAC_BRANA) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase, chloroplastic

EC=6.3.1.2
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene names
Name:GLN2
Synonyms:GLN
OrganismBrassica napus (Rape)
Taxonomic identifier3708 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration By similarity.

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4949Chloroplast Potential
Chain50 – 428379Glutamine synthetase, chloroplastic
PRO_0000011175

Amino acid modifications

Modified residue1041Phosphoserine By similarity

Experimental info

Sequence conflict501L → I in CAB72423. Ref.2
Sequence conflict821I → Y in CAB72423. Ref.2
Sequence conflict2631G → R in CAB72423. Ref.2
Sequence conflict3381S → I in CAB72423. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q42624 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A0B558C64FD9B18A

FASTA42847,345
        10         20         30         40         50         60 
MAQILAASPT CQMRLTKPSS IASSKLWNSV VLKQKKQSSS KVRSFKVMAL QSDNSTINRV 

        70         80         90        100        110        120 
ESLLNLDTKP FTDRIIAEYI WIGGSGIDLR SKSRTLEKPV EDPSELPKWN YDGSSTGQAP 

       130        140        150        160        170        180 
GEDSEVILYP QAIFRDPFRG GNNILVICDT YTPAGEPIPT NKRARAAEIF SNKKVNEEIP 

       190        200        210        220        230        240 
WFGIEQEYTL LQPNVNWPLG WPVGAYPGPQ GPYYCGVGAE KSWGRDISDA HYKACLYAGI 

       250        260        270        280        290        300 
NISGTNGEVM PGQWEFQVGP SVGIEAGDHV WCARYLLERI TEQAGVVLTL DPKPIEGDWN 

       310        320        330        340        350        360 
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHMEHISAY GEGNERRLTG KHETASIDQF 

       370        380        390        400        410        420 
SWGVANRGCS IRVGRDTEKK GKGYLEDRRP ASNMDPYIVT SLLAETTLLW EPTLEAEALA 


AQKLSLKV 

« Hide

References

[1]"Chloroplastic glutamine synthetase from Brassica napus."
Ochs G., Schock G., Wild A.
Plant Physiol. 103:303-304(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Leaf.
[2]"Cloning and Sequencing of genomic fragments coding for glutamine synthetase of Brassica napus."
Wojtyna S., Ochs G., Wild A.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Drakkar.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72751 mRNA. Translation: CAA51280.1.
AJ271909 Genomic DNA. Translation: CAB72423.1.
PIRS32228.

3D structure databases

ProteinModelPortalQ42624.
SMRQ42624. Positions 63-411.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNAC_BRANA
AccessionPrimary (citable) accession number: Q42624
Secondary accession number(s): Q9M429
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: March 19, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families