Reviewed, UniProtKB/Swiss-Prot Q42624 (GLNAC_BRANA)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase, chloroplastic
    EC=6.3.1.2
Alternative name(s):
    GS2
    Glutamate--ammonia ligase

Gene names

Name:	GLN2
Synonyms:	GLN

Organism

Brassica napus (Rape)

Taxonomic identifier

3708 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Brassica

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration By similarity.
Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 428	379	Glutamine synthetase, chloroplastic		PRO_0000011175
Experimental info
	Sequence conflict	50	1	L → I in CAB72423. Ref.2
	Sequence conflict	82	1	I → Y in CAB72423. Ref.2
	Sequence conflict	263	1	G → R in CAB72423. Ref.2
	Sequence conflict	338	1	S → I in CAB72423. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q42624-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: A0B558C64FD9B18A	FASTA	428	47,345
	10 20 30 40 50 60 MAQILAASPT CQMRLTKPSS IASSKLWNSV VLKQKKQSSS KVRSFKVMAL QSDNSTINRV 70 80 90 100 110 120 ESLLNLDTKP FTDRIIAEYI WIGGSGIDLR SKSRTLEKPV EDPSELPKWN YDGSSTGQAP 130 140 150 160 170 180 GEDSEVILYP QAIFRDPFRG GNNILVICDT YTPAGEPIPT NKRARAAEIF SNKKVNEEIP 190 200 210 220 230 240 WFGIEQEYTL LQPNVNWPLG WPVGAYPGPQ GPYYCGVGAE KSWGRDISDA HYKACLYAGI 250 260 270 280 290 300 NISGTNGEVM PGQWEFQVGP SVGIEAGDHV WCARYLLERI TEQAGVVLTL DPKPIEGDWN 310 320 330 340 350 360 GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHMEHISAY GEGNERRLTG KHETASIDQF 370 380 390 400 410 420 SWGVANRGCS IRVGRDTEKK GKGYLEDRRP ASNMDPYIVT SLLAETTLLW EPTLEAEALA AQKLSLKV

References

[1]	"Chloroplastic glutamine synthetase from Brassica napus." Ochs G., Schock G., Wild A. Plant Physiol. 103:303-304(1993) [PubMed: 7911583] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Cloning and Sequencing of genomic fragments coding for glutamine synthetase of Brassica napus." Wojtyna S., Ochs G., Wild A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Drakkar. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72751 mRNA. Translation: CAA51280.1. AJ271909 Genomic DNA. Translation: CAB72423.1.
PIR	S32228.
3D structure databases
SMR	Q42624. Positions 63-411.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.2. 393.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNAC_BRANA

Accession

Primary (citable) accession number: Q42624
Secondary accession number(s): Q9M429

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)