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Protein

Glutamine synthetase, chloroplastic

Gene

GLN2

Organism
Brassica napus (Rape)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The light-modulated chloroplast enzyme, encoded by a nuclear gene and expressed primarily in leaves, is responsible for the reassimilation of the ammonia generated by photorespiration.By similarity

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase, chloroplastic (EC:6.3.1.2)
Alternative name(s):
GS2
Glutamate--ammonia ligase
Gene namesi
Name:GLN2
Synonyms:GLN
OrganismiBrassica napus (Rape)
Taxonomic identifieri3708 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeBrassica

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 49ChloroplastSequence analysisAdd BLAST49
ChainiPRO_000001117550 – 428Glutamine synthetase, chloroplasticAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ42624.
SMRiQ42624.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK01915.
OMAiEVHISAY.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiView protein in InterPro
IPR008147. Gln_synt_b-grasp.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
PfamiView protein in Pfam
PF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
SMARTiView protein in SMART
SM01230. Gln-synt_C. 1 hit.
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiView protein in PROSITE
PS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42624-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQILAASPT CQMRLTKPSS IASSKLWNSV VLKQKKQSSS KVRSFKVMAL
60 70 80 90 100
QSDNSTINRV ESLLNLDTKP FTDRIIAEYI WIGGSGIDLR SKSRTLEKPV
110 120 130 140 150
EDPSELPKWN YDGSSTGQAP GEDSEVILYP QAIFRDPFRG GNNILVICDT
160 170 180 190 200
YTPAGEPIPT NKRARAAEIF SNKKVNEEIP WFGIEQEYTL LQPNVNWPLG
210 220 230 240 250
WPVGAYPGPQ GPYYCGVGAE KSWGRDISDA HYKACLYAGI NISGTNGEVM
260 270 280 290 300
PGQWEFQVGP SVGIEAGDHV WCARYLLERI TEQAGVVLTL DPKPIEGDWN
310 320 330 340 350
GAGCHTNYST KSMREDGGFE VIKKAILNLS LRHMEHISAY GEGNERRLTG
360 370 380 390 400
KHETASIDQF SWGVANRGCS IRVGRDTEKK GKGYLEDRRP ASNMDPYIVT
410 420
SLLAETTLLW EPTLEAEALA AQKLSLKV
Length:428
Mass (Da):47,345
Last modified:November 1, 1996 - v1
Checksum:iA0B558C64FD9B18A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50L → I in CAB72423 (Ref. 2) Curated1
Sequence conflicti82I → Y in CAB72423 (Ref. 2) Curated1
Sequence conflicti263G → R in CAB72423 (Ref. 2) Curated1
Sequence conflicti338S → I in CAB72423 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72751 mRNA. Translation: CAA51280.1.
AJ271909 Genomic DNA. Translation: CAB72423.1.
PIRiS32228.
RefSeqiNP_001302944.1. NM_001316015.1.
UniGeneiBna.2374.

Genome annotation databases

GeneIDi106424972.
KEGGibna:106424972.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGLNAC_BRANA
AccessioniPrimary (citable) accession number: Q42624
Secondary accession number(s): Q9M429
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families