ID   PALY_BROFI              Reviewed;         703 AA.
AC   Q42609;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 57.
DE   RecName: Full=Phenylalanine ammonia-lyase;
DE            EC=4.3.1.24;
GN   Name=PAL;
OS   Bromheadia finlaysoniana (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; higher Epidendroideae; Cymbidieae; Bromheadiinae;
OC   Bromheadia.
OX   NCBI_TaxID=41205;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Liew C.-F., Goh C.-J., Loh C.-S., Lim S.-H.;
RT   "Cloning and nucleotide sequence of a cDNA encoding phenylalanine
RT   ammonia-lyase from Bromheadia finlaysoniana (Lindl.) Rchb.f.";
RL   (er) Plant Gene Register PGR96-087.
CC   -!- FUNCTION: This is a key enzyme of plant metabolism catalyzing the
CC       first reaction in the biosynthesis from L-phenylalanine of a wide
CC       variety of natural products based on the phenylpropane skeleton.
CC   -!- CATALYTIC ACTIVITY: L-phenylalanine = trans-cinnamate + ammonia.
CC   -!- PATHWAY: Phenylpropanoid metabolism; cinnamic acid biosynthesis;
CC       trans-cinnamic acid from L-phenylalanine: step 1/1.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- PTM: Contains an active site 4-methylidene-imidazol-5-one (MIO),
CC       which is formed autocatalytically by cyclization and dehydration
CC       of residues Ala-Ser-Gly (By similarity).
CC   -!- SIMILARITY: Belongs to the PAL/histidase family.
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DR   EMBL; X99997; CAA68256.1; -; mRNA.
DR   HSSP; P21310; 1GK2.
DR   SMR; Q42609; 15-703.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016211; F:ammonia ligase activity; IEA:InterPro.
DR   GO; GO:0016841; F:ammonia-lyase activity; IEA:InterPro.
DR   GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR   GO; GO:0006559; P:L-phenylalanine catabolic process; IEA:InterPro.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001106; Phe/His_NH3-lyase.
DR   InterPro; IPR005922; Phe_NH3-lyase.
DR   Pfam; PF00221; PAL; 1.
DR   TIGRFAMs; TIGR01226; phe_am_lyase; 1.
DR   PROSITE; PS00488; PAL_HISTIDASE; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Lyase; Phenylpropanoid metabolism.
FT   CHAIN         1    703       Phenylalanine ammonia-lyase.
FT                                /FTId=PRO_0000215386.
FT   MOD_RES     191    191       2,3-didehydroalanine (Ser) (By
FT                                similarity).
FT   CROSSLNK    190    192       5-imidazolinone (Ala-Gly) (By
FT                                similarity).
SQ   SEQUENCE   703 AA;  76026 MW;  9E848050F97B25AC CRC64;
     MEVSKENGLC LQGRDPLNWG AAAAELQGSH LDEVKKMVEE FRRPVVKLEG VKLKISQVAA
     VAFGGGASAV ELAESARAGV KASSDWVLES VDKGTDSYGV TTGFGATSHR RTKQGGALQK
     ELIKFLNAGI FGSGNSNTLP SAATRAAMLV RINTLLQGYS GIRFEILKAI ATLLNKNITP
     CLPLRGTITA SGDLVPLSYL AGILTGRPNS KARTPNGSTV DATTAFRLAG ISSGFFDLQP
     KEGLALVNGT AVGSGVASIV LFETNILAVM AELLSALFCE VMQGKPEFTD HLTHKLKHHP
     GQIEAAAVME HILEGSSYMK MAKKLHEMDP LQKPKQDRYA LRTSPQWLGP QIEVIRAATK
     SIEREINSVN DNPLIDVSRN KALHGGNFQG TPIGVSMDNT RLAIAAIGKL MFAQFSELVN
     DFYNNGLPSN LSSGRNPSLD YGFKGAEIAM ASYCSELQAL ANPVTNHVQS AEQHNQDVNS
     LGLISSRKTA EAVDILKLMS TTFLVGLCQA VDLRHLEENL KNAVKNTVSQ VAKRVLTMGV
     NGELHPSRFC EKDLIKVIDR EYVFAYADDP CSSTYPLMQK LRAVIVEHAL NNGVKEKDSN
     TSIFQKISSF ENELKAALPK EVEAARAEFE NGSPAIENRI KDCRSYPLYK FVKEVGSGFL
     TGEKVVSPGE EFDKVFNAIC EGKAIDPMLD CLKEWNGAPL PIC
//