Reviewed, UniProtKB/Swiss-Prot Q42609 (PALY_BROFI)

Names and origin

Protein names

Recommended name:
    Phenylalanine ammonia-lyase
    EC=4.3.1.24

Gene names

Name:

PAL

Organism

Bromheadia finlaysoniana (Orchid)

Taxonomic identifier

41205 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Orchidaceae › Epidendroideae › higher Epidendroideae › Cymbidieae › Bromheadiinae › Bromheadia

Protein attributes

Sequence length	703 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity	L-phenylalanine = trans-cinnamate + ammonia.
Pathway	Phenylpropanoid metabolism; cinnamic acid biosynthesis; trans-cinnamic acid from L-phenylalanine: step 1/1.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro ammonia-lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 703	703	Phenylalanine ammonia-lyase		PRO_0000215386
Amino acid modifications
	Modified residue	191	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	190 ↔ 192		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42609-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9E848050F97B25AC	FASTA	703	76,026
	10 20 30 40 50 60 MEVSKENGLC LQGRDPLNWG AAAAELQGSH LDEVKKMVEE FRRPVVKLEG VKLKISQVAA 70 80 90 100 110 120 VAFGGGASAV ELAESARAGV KASSDWVLES VDKGTDSYGV TTGFGATSHR RTKQGGALQK 130 140 150 160 170 180 ELIKFLNAGI FGSGNSNTLP SAATRAAMLV RINTLLQGYS GIRFEILKAI ATLLNKNITP 190 200 210 220 230 240 CLPLRGTITA SGDLVPLSYL AGILTGRPNS KARTPNGSTV DATTAFRLAG ISSGFFDLQP 250 260 270 280 290 300 KEGLALVNGT AVGSGVASIV LFETNILAVM AELLSALFCE VMQGKPEFTD HLTHKLKHHP 310 320 330 340 350 360 GQIEAAAVME HILEGSSYMK MAKKLHEMDP LQKPKQDRYA LRTSPQWLGP QIEVIRAATK 370 380 390 400 410 420 SIEREINSVN DNPLIDVSRN KALHGGNFQG TPIGVSMDNT RLAIAAIGKL MFAQFSELVN 430 440 450 460 470 480 DFYNNGLPSN LSSGRNPSLD YGFKGAEIAM ASYCSELQAL ANPVTNHVQS AEQHNQDVNS 490 500 510 520 530 540 LGLISSRKTA EAVDILKLMS TTFLVGLCQA VDLRHLEENL KNAVKNTVSQ VAKRVLTMGV 550 560 570 580 590 600 NGELHPSRFC EKDLIKVIDR EYVFAYADDP CSSTYPLMQK LRAVIVEHAL NNGVKEKDSN 610 620 630 640 650 660 TSIFQKISSF ENELKAALPK EVEAARAEFE NGSPAIENRI KDCRSYPLYK FVKEVGSGFL 670 680 690 700 TGEKVVSPGE EFDKVFNAIC EGKAIDPMLD CLKEWNGAPL PIC

References

[1]	"Cloning and nucleotide sequence of a cDNA encoding phenylalanine ammonia-lyase from Bromheadia finlaysoniana (Lindl.) Rchb.f." Liew C.-F., Goh C.-J., Loh C.-S., Lim S.-H. Plant Gene Register PGR96-087 Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99997 mRNA. Translation: CAA68256.1.
3D structure databases
HSSP	HSSP built from PDB template 1GK2 based on UniProtKB P21310.
SMR	Q42609. Positions 15-703.
ModBase	Search...
Family and domain databases
InterPro	IPR001106. Phe/His_NH3-lyase. IPR005922. Phe_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01226. phe_am_lyase. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PALY_BROFI

Accession

Primary (citable) accession number: Q42609

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)