Q42605 (GALE1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 4-epimerase 1
EC=5.1.3.2

Alternative name(s):
Galactowaldenase 1
UDP-galactose 4-epimerase 1

Gene names

Ordered Locus Names:	At1g12780
ORF Names:	F13K23.3

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucose = UDP-galactose.
Pathway	Carbohydrate metabolism; galactose metabolism.
Tissue specificity	Ubiquitously expressed. Highest expression in stems and roots.
Miscellaneous	Does not seem to need NAD for activity.
Sequence similarities	Belongs to the sugar epimerase family.
Biophysicochemical properties	Kinetic parameters: KM=0.11 mM for UDP-glucose Vmax=3.96 mmol/min/mg enzyme pH dependence: Optimum pH is 7.0-9.5.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	NAD
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from mutant phenotype. Source: TAIR
Cellular component	cytosol Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	UDP-glucose 4-epimerase activity Inferred from direct assay Ref.1. Source: TAIR coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from physical interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	UDP-glucose 4-epimerase 1		PRO_0000183193
Regions
	Nucleotide binding	8 – 39	32	NAD By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	134	1	Substrate By similarity
Experimental info
	Sequence conflict	102 – 103	2	NP → KG in CAA90941. Ref.1
	Sequence conflict	341	1	N → F in CAA90941. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q42605 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: FDE640FB26A1DD1D	FASTA	351	39,158
	10 20 30 40 50 60 MGSSVEQNIL VTGGAGFIGT HTVVQLLKDG FKVSIIDNFD NSVIEAVDRV RELVGPDLSK 70 80 90 100 110 120 KLDFNLGDLR NKGDIEKLFS KQRFDAVIHF AGLKAVGESV ENPRRYFDNN LVGTINLYET 130 140 150 160 170 180 MAKYNCKMMV FSSSATVYGQ PEKIPCMEDF ELKAMNPYGR TKLFLEEIAR DIQKAEPEWR 190 200 210 220 230 240 IILLRYFNPV GAHESGSIGE DPKGIPNNLM PYIQQVAVGR LPELNVYGHD YPTEDGSAVR 250 260 270 280 290 300 DYIHVMDLAD GHIAALRKLF ADPKIGCTAY NLGTGQGTSV LEMVAAFEKA SGKKIPIKLC 310 320 330 340 350 PRRSGDATAV YASTEKAEKE LGWKAKYGVD EMCRDQWKWA NNNPWGYQNK L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli." Doermann P., Benning C. Arch. Biochem. Biophys. 327:27-34(1996) [PubMed: 8615692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z54214 mRNA. Translation: CAA90941.1. AC012187 Genomic DNA. Translation: AAF78483.1. CP002684 Genomic DNA. Translation: AEE28928.1. AF334724 mRNA. Translation: AAG50102.1. AY054207 mRNA. Translation: AAL06868.1. AY120709 mRNA. Translation: AAM53267.1. BT000032 mRNA. Translation: AAN15351.1.
IPI	IPI00533120.
PIR	B86261. S62783.
RefSeq	NP_172738.1. NM_101148.3.
UniGene	At.20506. At.27284. At.74803.
3D structure databases
ProteinModelPortal	Q42605.
SMR	Q42605. Positions 6-350.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42605. 2 interactions.
STRING	Q42605.
Proteomic databases
PRIDE	Q42605.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G12780.1; AT1G12780.1; AT1G12780.
GeneID	837834.
GenomeReviews	Gene locus AT1G12780 in contig CT485782_GR.
KEGG	ath:AT1G12780.
NMPDR	fig|3702.1.peg.1545.
Organism-specific databases
TAIR	At1g12780.
Phylogenomic databases
eggNOG	KOG1371.
GeneTree	EPGT00070000028397.
HOGENOM	HBG755066.
InParanoid	Q42605.
OMA	HESGSIG.
PhylomeDB	Q42605.
ProtClustDB	PLN02240.
Enzyme and pathway databases
BioCyc	ARA:AT1G12780-MONOMER. MetaCyc:AT1G12780-MONOMER.
Gene expression databases
ArrayExpress	Q42605.
Genevestigator	Q42605.
GermOnline	AT1G12780. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR005886. GalE. IPR016040. NAD(P)-bd_dom. IPR008089. Nuc_sugar_epim. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01784.
PANTHER	PTHR10366:SF39. GalE. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
PRINTS	PR01713. NUCEPIMERASE.
TIGRFAMs	TIGR01179. GalE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALE1_ARATH

Accession

Primary (citable) accession number: Q42605
Secondary accession number(s): Q9LPX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	December 14, 2011
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program