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Reviewed, UniProtKB/Swiss-Prot Q42605 (GALE1_ARATH)

Last modified June 16, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    UDP-glucose 4-epimerase 1
    EC=5.1.3.2
Alternative name(s):
    UDP-galactose 4-epimerase 1
    Galactowaldenase 1
Gene names
Ordered Locus Names: At1g12780
ORF Names: F13K23.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

UDP-glucose = UDP-galactose.

Pathway

Carbohydrate metabolism; galactose metabolism.

Tissue specificity

Ubiquitously expressed. Highest expression in stems and roots.

Miscellaneous

Does not seem to need NAD for activity.

Sequence similarities

Belongs to the sugar epimerase family.

Biophysicochemical properties

Kinetic parameters:

KM=0.11 mM for UDP-glucose

Vmax=3.96 mmol/min/mg enzyme

pH dependence:

Optimum pH is 7.0-9.5.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351UDP-glucose 4-epimerase 1
PRO_0000183193

Regions

Nucleotide binding8 – 3932NAD By similarity

Sites

Active site1581Proton acceptor By similarity
Binding site1341Substrate By similarity

Experimental info

Sequence conflict102 – 1032NP → KG in CAA90941. Ref.1
Sequence conflict3411N → F in CAA90941. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q42605-1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: FDE640FB26A1DD1D

FASTA35139,158
        10         20         30         40         50         60 
MGSSVEQNIL VTGGAGFIGT HTVVQLLKDG FKVSIIDNFD NSVIEAVDRV RELVGPDLSK 

        70         80         90        100        110        120 
KLDFNLGDLR NKGDIEKLFS KQRFDAVIHF AGLKAVGESV ENPRRYFDNN LVGTINLYET 

       130        140        150        160        170        180 
MAKYNCKMMV FSSSATVYGQ PEKIPCMEDF ELKAMNPYGR TKLFLEEIAR DIQKAEPEWR 

       190        200        210        220        230        240 
IILLRYFNPV GAHESGSIGE DPKGIPNNLM PYIQQVAVGR LPELNVYGHD YPTEDGSAVR 

       250        260        270        280        290        300 
DYIHVMDLAD GHIAALRKLF ADPKIGCTAY NLGTGQGTSV LEMVAAFEKA SGKKIPIKLC 

       310        320        330        340        350 
PRRSGDATAV YASTEKAEKE LGWKAKYGVD EMCRDQWKWA NNNPWGYQNK L 

« Hide

References

« Hide 'large scale' references
[1]"Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli."
Doermann P., Benning C.
Arch. Biochem. Biophys. 327:27-34(1996) [PubMed: 8615692] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z54214 mRNA. Translation: CAA90941.1.
AC012187 Genomic DNA. Translation: AAF78483.1.
AF334724 mRNA. Translation: AAG50102.1.
AY054207 mRNA. Translation: AAL06868.1.
AY120709 mRNA. Translation: AAM53267.1.
BT000032 mRNA. Translation: AAN15351.1.
IPIIPI00533120.
PIRB86261.
S62783.
RefSeqNP_172738.1.
UniGeneAt.20506

3D structure databases

HSSPHSSP built from PDB template 1EK6 based on UniProtKB Q14376.
ModBaseSearch...

Proteomic databases

PRIDEQ42605.

Genome annotation databases

GeneID837834.
GenomeReviewsGene locus AT1G12780 in contig CT485782_GR.
KEGGath:AT1G12780.
NMPDRfig|3702.1.peg.1545.

Organism-specific databases

TAIRAt1g12780.

Phylogenomic databases

OMAQ42605. RDQWKWA.

Enzyme and pathway databases

BioCycMetaCyc:AT1G12780-MON.
BRENDA5.1.3.2. 302.

Gene expression databases

ArrayExpressQ42605.
GermOnlineAT1G12780. Arabidopsis thaliana.

Family and domain databases

InterProIPR001509. Epimerase_deHydtase.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
IPR008089. Nuc_sugar_epim.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10366:SF39. GalE. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
PRINTSPR01713. NUCEPIMERASE.
TIGRFAMsTIGR01179. galE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGALE1_ARATH
AccessionPrimary (citable) accession number: Q42605
Secondary accession number(s): Q9LPX1
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents