Q42605 (GALE1_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 102.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: UDP-glucose 4-epimerase 1 EC=5.1.3.2 Alternative name(s): Galactowaldenase 1 UDP-galactose 4-epimerase 1 | ||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 351 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Catalytic activity | UDP-glucose = UDP-galactose. |
| Pathway | |
| Tissue specificity | Ubiquitously expressed. Highest expression in stems and roots. |
| Miscellaneous | Does not seem to need NAD for activity. |
| Sequence similarities | Belongs to the sugar epimerase family. |
| Biophysicochemical properties | Kinetic parameters: KM=0.11 mM for UDP-glucose Vmax=3.96 mmol/min/mg enzyme pH dependence: Optimum pH is 7.0-9.5. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Galactose metabolism |
| Ligand | NAD |
| Molecular function | Isomerase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW response to stressInferred from mutant phenotype. Source: TAIR |
| Cellular component | cytosol Inferred from direct assay. Source: TAIR plasma membraneInferred from direct assay. Source: TAIR |
| Molecular function | UDP-glucose 4-epimerase activity Inferred from direct assay Ref.1. Source: TAIR coenzyme bindingInferred from electronic annotation. Source: InterPro nucleotide bindingInferred from electronic annotation. Source: InterPro protein dimerization activityInferred from physical interaction. Source: TAIR |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 351 | 351 | UDP-glucose 4-epimerase 1 | PRO_0000183193 | |||||
Regions | |||||||||
| Nucleotide binding | 8 – 39 | 32 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 158 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 134 | 1 | Substrate By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 102 – 103 | 2 | NP → KG in CAA90941. Ref.1 | ||||||
| Sequence conflict | 341 | 1 | N → F in CAA90941. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Functional expression of uridine 5'-diphospho-glucose 4-epimerase (EC 5.1.3.2) from Arabidopsis thaliana in Saccharomyces cerevisiae and Escherichia coli." Doermann P., Benning C. Arch. Biochem. Biophys. 327:27-34(1996) [PubMed: 8615692] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Columbia. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | Z54214 mRNA. Translation: CAA90941.1. AC012187 Genomic DNA. Translation: AAF78483.1. CP002684 Genomic DNA. Translation: AEE28928.1. AF334724 mRNA. Translation: AAG50102.1. AY054207 mRNA. Translation: AAL06868.1. AY120709 mRNA. Translation: AAM53267.1. BT000032 mRNA. Translation: AAN15351.1. |
| IPI | IPI00533120. |
| PIR | B86261. S62783. |
| RefSeq | NP_172738.1. NM_101148.3. |
| UniGene | At.20506. At.27284. At.74803. |
3D structure databases | |
| ProteinModelPortal | Q42605. |
| SMR | Q42605. Positions 6-350. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q42605. 2 interactions. |
| STRING | Q42605. |
Proteomic databases | |
| PRIDE | Q42605. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT1G12780.1; AT1G12780.1; AT1G12780. |
| GeneID | 837834. |
| GenomeReviews | Gene locus AT1G12780 in contig CT485782_GR. |
| KEGG | ath:AT1G12780. |
| NMPDR | fig|3702.1.peg.1545. |
Organism-specific databases | |
| TAIR | At1g12780. |
Phylogenomic databases | |
| eggNOG | KOG1371. |
| GeneTree | EPGT00070000028397. |
| HOGENOM | HBG755066. |
| InParanoid | Q42605. |
| OMA | HESGSIG. |
| PhylomeDB | Q42605. |
| ProtClustDB | PLN02240. |
Enzyme and pathway databases | |
| BioCyc | ARA:AT1G12780-MONOMER. MetaCyc:AT1G12780-MONOMER. |
Gene expression databases | |
| ArrayExpress | Q42605. |
| Genevestigator | Q42605. |
| GermOnline | AT1G12780. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001509. Epimerase_deHydtase. IPR005886. GalE. IPR016040. NAD(P)-bd_dom. IPR008089. Nuc_sugar_epim. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K01784. |
| PANTHER | PTHR10366:SF39. GalE. 1 hit. |
| Pfam | PF01370. Epimerase. 1 hit. [Graphical view] |
| PRINTS | PR01713. NUCEPIMERASE. |
| TIGRFAMs | TIGR01179. GalE. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | GALE1_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q42605 Secondary accession number(s): Q9LPX1 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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