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Protein

Carbamoyl-phosphate synthase large chain, chloroplastic

Gene

CARB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in arginine biosynthesis. Required for mesophyll development.1 Publication

Miscellaneous

The ven3-1, ven3-2, ven3-3 and ven3-4 phenotypes are rescued by exogenous application of citrulline, an arginine precursor.1 Publication

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi377Magnesium or manganese 1By similarity1
Metal bindingi391Magnesium or manganese 1By similarity1
Metal bindingi391Magnesium or manganese 2By similarity1
Metal bindingi393Magnesium or manganese 2By similarity1
Metal bindingi933Magnesium or manganese 3By similarity1
Metal bindingi946Magnesium or manganese 3By similarity1
Metal bindingi946Magnesium or manganese 4By similarity1
Metal bindingi948Magnesium or manganese 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi251 – 308ATPBy similarityAdd BLAST58
Nucleotide bindingi808 – 865ATPBy similarityAdd BLAST58

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G29900-MONOMER
ReactomeiR-ATH-500753 Pyrimidine biosynthesis
R-ATH-70635 Urea cycle
UniPathwayiUPA00068; UER00171
UPA00070; UER00115

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chain, chloroplastic (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Protein VENOSA 6
Gene namesi
Name:CARB
Synonyms:VEN3
Ordered Locus Names:At1g29900
ORF Names:F1N18.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G29900
TAIRilocus:2019302 AT1G29900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi149P → L in ven3-2; reduced plant size and reticulate leaf phenotype. 1 Publication1
Mutagenesisi587G → E in ven3-3; reticulate leaf phenotype. 1 Publication1
Mutagenesisi844A → T in ven3-4; reduced plant size and reticulate leaf phenotype. 1 Publication1
Mutagenesisi1014P → L in ven3-1; reticulate leaf phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 62ChloroplastSequence analysisAdd BLAST62
ChainiPRO_000042307663 – 1187Carbamoyl-phosphate synthase large chain, chloroplasticAdd BLAST1125

Proteomic databases

PaxDbiQ42601
PRIDEiQ42601
ProMEXiQ42601

PTM databases

iPTMnetiQ42601

Expressioni

Tissue specificityi

Expressed in roots and leaves.1 Publication

Inductioni

By phosphate starvation in shoot.1 Publication

Gene expression databases

ExpressionAtlasiQ42601 baseline and differential
GenevisibleiQ42601 AT

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

BioGridi25103, 2 interactors
IntActiQ42601, 1 interactor
STRINGi3702.AT1G29900.1

Structurei

3D structure databases

ProteinModelPortaliQ42601
SMRiQ42601
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini224 – 420ATP-grasp 1Add BLAST197
Domaini782 – 975ATP-grasp 2Add BLAST194
Domaini1042 – 1183MGS-likePROSITE-ProRule annotationAdd BLAST142

Sequence similaritiesi

Belongs to the CarB family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0370 Eukaryota
COG0458 LUCA
HOGENOMiHOG000234582
InParanoidiQ42601
KOiK01955
OMAiAVFPFNK
OrthoDBiEOG093600HH
PhylomeDBiQ42601

Family and domain databases

CDDicd01424 MGS_CPS_II, 1 hit
Gene3Di1.10.1030.10, 1 hit
3.40.50.1380, 1 hit
HAMAPiMF_01210_A CPSase_L_chain_A, 1 hit
MF_01210_B CPSase_L_chain_B, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR006275 CarbamoylP_synth_lsu
IPR005480 CarbamoylP_synth_lsu_oligo
IPR036897 CarbamoylP_synth_lsu_oligo_sf
IPR005479 CbamoylP_synth_lsu-like_ATP-bd
IPR005483 CbamoylP_synth_lsu_CPSase_dom
IPR011607 MGS-like_dom
IPR036914 MGS-like_dom_sf
IPR033937 MGS_CPS_CarB
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF02786 CPSase_L_D2, 2 hits
PF02787 CPSase_L_D3, 1 hit
PF02142 MGS, 1 hit
PRINTSiPR00098 CPSASE
SMARTiView protein in SMART
SM01096 CPSase_L_D3, 1 hit
SM00851 MGS, 1 hit
SUPFAMiSSF48108 SSF48108, 1 hit
SSF52335 SSF52335, 1 hit
SSF52440 SSF52440, 2 hits
TIGRFAMsiTIGR01369 CPSaseII_lrg, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 2 hits
PS00866 CPSASE_1, 2 hits
PS00867 CPSASE_2, 2 hits
PS51855 MGS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNHCLELSS NCSSIFASSK SNPRFSPSKL SYSTFFSRSA IYYRSKPKQA
60 70 80 90 100
SSSSSFSTFP PCLNRKSSLT HVLKPVSELA DTTTKPFSPE IVGKRTDLKK
110 120 130 140 150
IMILGAGPIV IGQACEFDYS GTQACKALRE EGYEVILINS NPATIMTDPE
160 170 180 190 200
TANRTYIAPM TPELVEQVIE KERPDALLPT MGGQTALNLA VALAESGALE
210 220 230 240 250
KYGVELIGAK LGAIKKAEDR ELFKDAMKNI GLKTPPSGIG TTLDECFDIA
260 270 280 290 300
EKIGEFPLII RPAFTLGGTG GGIAYNKEEF ESICKSGLAA SATSQVLVEK
310 320 330 340 350
SLLGWKEYEL EVMRDLADNV VIICSIENID PMGVHTGDSI TVAPAQTLTD
360 370 380 390 400
REYQRLRDYS IAIIREIGVE CGGSNVQFAV NPVDGEVMII EMNPRVSRSS
410 420 430 440 450
ALASKATGFP IAKMAAKLSV GYTLDQIPND ITRKTPASFE PSIDYVVTKI
460 470 480 490 500
PRFAFEKFPG SQPLLTTQMK SVGESMALGR TFQESFQKAL RSLECGFSGW
510 520 530 540 550
GCAKIKELDW DWDQLKYSLR VPNPDRIHAI YAAMKKGMKI DEIYELSMVD
560 570 580 590 600
KWFLTQLKEL VDVEQYLMSG TLSEITKEDL YEVKKRGFSD KQIAFATKTT
610 620 630 640 650
EEEVRTKRIS LGVVPSYKRV DTCAAEFEAH TPYMYSSYDV ECESAPNNKK
660 670 680 690 700
KVLILGGGPN RIGQGIEFDY CCCHTSFALQ DAGYETIMLN SNPETVSTDY
710 720 730 740 750
DTSDRLYFEP LTIEDVLNVI DLEKPDGIIV QFGGQTPLKL ALPIKHYLDK
760 770 780 790 800
HMPMSLSGAG PVRIWGTSPD SIDAAEDRER FNAILDELKI EQPKGGIAKS
810 820 830 840 850
EADALAIAKE VGYPVVVRPS YVLGGRAMEI VYDDSRLITY LENAVQVDPE
860 870 880 890 900
RPVLVDKYLS DAIEIDVDTL TDSYGNVVIG GIMEHIEQAG VHSGDSACML
910 920 930 940 950
PTQTIPASCL QTIRTWTTKL AKKLNVCGLM NCQYAITTSG DVFLLEANPR
960 970 980 990 1000
ASRTVPFVSK AIGHPLAKYA ALVMSGKSLK DLNFEKEVIP KHVSVKEAVF
1010 1020 1030 1040 1050
PFEKFQGCDV ILGPEMRSTG EVMSISSEFS SAFAMAQIAA GQKLPLSGTV
1060 1070 1080 1090 1100
FLSLNDMTKP HLEKIAVSFL ELGFKIVATS GTAHFLELKG IPVERVLKLH
1110 1120 1130 1140 1150
EGRPHAADMV ANGQIHLMLI TSSGDALDQK DGRQLRQMAL AYKVPVITTV
1160 1170 1180
AGALATAEGI KSLKSSAIKM TALQDFFEVK NVSSLLV
Length:1,187
Mass (Da):129,957
Last modified:November 1, 1996 - v1
Checksum:i328072F4A5B671DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40341 mRNA Translation: AAB67843.1
AC008030 Genomic DNA Translation: AAG10606.1
CP002684 Genomic DNA Translation: AEE31148.1
AF367268 mRNA Translation: AAK56257.1
AY133548 mRNA Translation: AAM91378.1
PIRiF86422
RefSeqiNP_564338.1, NM_102730.2
UniGeneiAt.10370

Genome annotation databases

EnsemblPlantsiAT1G29900.1; AT1G29900.1; AT1G29900
GeneIDi839868
GrameneiAT1G29900.1; AT1G29900.1; AT1G29900
KEGGiath:AT1G29900

Similar proteinsi

Entry informationi

Entry nameiCARB_ARATH
AccessioniPrimary (citable) accession number: Q42601
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health