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Protein

Carbamoyl-phosphate synthase large chain, chloroplastic

Gene

CARB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in arginine biosynthesis. Required for mesophyll development.1 Publication

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 4 Mg2+ or Mn2+ ions per subunit.By similarity

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes carbamoyl phosphate from bicarbonate.
Proteins known to be involved in this subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes carbamoyl phosphate from bicarbonate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase large chain, chloroplastic (CARB), Carbamoyl-phosphate synthase small chain, chloroplastic (CARA)
  2. Aspartate carbamoyltransferase, chloroplastic (PYRB)
  3. Dihydroorotase, mitochondrial (PYR4)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi377 – 3771Magnesium or manganese 1By similarity
Metal bindingi391 – 3911Magnesium or manganese 1By similarity
Metal bindingi391 – 3911Magnesium or manganese 2By similarity
Metal bindingi393 – 3931Magnesium or manganese 2By similarity
Metal bindingi933 – 9331Magnesium or manganese 3By similarity
Metal bindingi946 – 9461Magnesium or manganese 3By similarity
Metal bindingi946 – 9461Magnesium or manganese 4By similarity
Metal bindingi948 – 9481Magnesium or manganese 4By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi251 – 30858ATPBy similarityAdd
BLAST
Nucleotide bindingi808 – 86558ATPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G29900-MONOMER.
ReactomeiR-ATH-500753. Pyrimidine biosynthesis.
R-ATH-70635. Urea cycle.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase large chain, chloroplastic (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase ammonia chain
Protein VENOSA 6
Gene namesi
Name:CARB
Synonyms:VEN3
Ordered Locus Names:At1g29900
ORF Names:F1N18.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G29900.

Subcellular locationi

GO - Cellular componenti

  • carbamoyl-phosphate synthase complex Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi149 – 1491P → L in ven3-2; reduced plant size and reticulate leaf phenotype. 1 Publication
Mutagenesisi587 – 5871G → E in ven3-3; reticulate leaf phenotype. 1 Publication
Mutagenesisi844 – 8441A → T in ven3-4; reduced plant size and reticulate leaf phenotype. 1 Publication
Mutagenesisi1014 – 10141P → L in ven3-1; reticulate leaf phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6262ChloroplastSequence analysisAdd
BLAST
Chaini63 – 11871125Carbamoyl-phosphate synthase large chain, chloroplasticPRO_0000423076Add
BLAST

Proteomic databases

PaxDbiQ42601.
PRIDEiQ42601.
ProMEXiQ42601.

PTM databases

iPTMnetiQ42601.

Expressioni

Tissue specificityi

Expressed in roots and leaves.1 Publication

Inductioni

By phosphate starvation in shoot.1 Publication

Gene expression databases

GenevisibleiQ42601. AT.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.By similarity

Protein-protein interaction databases

BioGridi25103. 2 interactions.
IntActiQ42601. 1 interaction.
STRINGi3702.AT1G29900.1.

Structurei

3D structure databases

ProteinModelPortaliQ42601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini224 – 420197ATP-grasp 1Add
BLAST
Domaini782 – 975194ATP-grasp 2Add
BLAST

Sequence similaritiesi

Belongs to the CarB family.Curated
Contains 2 ATP-grasp domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG0370. Eukaryota.
COG0458. LUCA.
HOGENOMiHOG000234582.
InParanoidiQ42601.
KOiK01955.
OMAiCCHTSFA.
OrthoDBiEOG093600HH.
PhylomeDBiQ42601.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNHCLELSS NCSSIFASSK SNPRFSPSKL SYSTFFSRSA IYYRSKPKQA
60 70 80 90 100
SSSSSFSTFP PCLNRKSSLT HVLKPVSELA DTTTKPFSPE IVGKRTDLKK
110 120 130 140 150
IMILGAGPIV IGQACEFDYS GTQACKALRE EGYEVILINS NPATIMTDPE
160 170 180 190 200
TANRTYIAPM TPELVEQVIE KERPDALLPT MGGQTALNLA VALAESGALE
210 220 230 240 250
KYGVELIGAK LGAIKKAEDR ELFKDAMKNI GLKTPPSGIG TTLDECFDIA
260 270 280 290 300
EKIGEFPLII RPAFTLGGTG GGIAYNKEEF ESICKSGLAA SATSQVLVEK
310 320 330 340 350
SLLGWKEYEL EVMRDLADNV VIICSIENID PMGVHTGDSI TVAPAQTLTD
360 370 380 390 400
REYQRLRDYS IAIIREIGVE CGGSNVQFAV NPVDGEVMII EMNPRVSRSS
410 420 430 440 450
ALASKATGFP IAKMAAKLSV GYTLDQIPND ITRKTPASFE PSIDYVVTKI
460 470 480 490 500
PRFAFEKFPG SQPLLTTQMK SVGESMALGR TFQESFQKAL RSLECGFSGW
510 520 530 540 550
GCAKIKELDW DWDQLKYSLR VPNPDRIHAI YAAMKKGMKI DEIYELSMVD
560 570 580 590 600
KWFLTQLKEL VDVEQYLMSG TLSEITKEDL YEVKKRGFSD KQIAFATKTT
610 620 630 640 650
EEEVRTKRIS LGVVPSYKRV DTCAAEFEAH TPYMYSSYDV ECESAPNNKK
660 670 680 690 700
KVLILGGGPN RIGQGIEFDY CCCHTSFALQ DAGYETIMLN SNPETVSTDY
710 720 730 740 750
DTSDRLYFEP LTIEDVLNVI DLEKPDGIIV QFGGQTPLKL ALPIKHYLDK
760 770 780 790 800
HMPMSLSGAG PVRIWGTSPD SIDAAEDRER FNAILDELKI EQPKGGIAKS
810 820 830 840 850
EADALAIAKE VGYPVVVRPS YVLGGRAMEI VYDDSRLITY LENAVQVDPE
860 870 880 890 900
RPVLVDKYLS DAIEIDVDTL TDSYGNVVIG GIMEHIEQAG VHSGDSACML
910 920 930 940 950
PTQTIPASCL QTIRTWTTKL AKKLNVCGLM NCQYAITTSG DVFLLEANPR
960 970 980 990 1000
ASRTVPFVSK AIGHPLAKYA ALVMSGKSLK DLNFEKEVIP KHVSVKEAVF
1010 1020 1030 1040 1050
PFEKFQGCDV ILGPEMRSTG EVMSISSEFS SAFAMAQIAA GQKLPLSGTV
1060 1070 1080 1090 1100
FLSLNDMTKP HLEKIAVSFL ELGFKIVATS GTAHFLELKG IPVERVLKLH
1110 1120 1130 1140 1150
EGRPHAADMV ANGQIHLMLI TSSGDALDQK DGRQLRQMAL AYKVPVITTV
1160 1170 1180
AGALATAEGI KSLKSSAIKM TALQDFFEVK NVSSLLV
Length:1,187
Mass (Da):129,957
Last modified:November 1, 1996 - v1
Checksum:i328072F4A5B671DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40341 mRNA. Translation: AAB67843.1.
AC008030 Genomic DNA. Translation: AAG10606.1.
CP002684 Genomic DNA. Translation: AEE31148.1.
AF367268 mRNA. Translation: AAK56257.1.
AY133548 mRNA. Translation: AAM91378.1.
PIRiF86422.
RefSeqiNP_564338.1. NM_102730.1.
UniGeneiAt.10370.

Genome annotation databases

EnsemblPlantsiAT1G29900.1; AT1G29900.1; AT1G29900.
GeneIDi839868.
GrameneiAT1G29900.1; AT1G29900.1; AT1G29900.
KEGGiath:AT1G29900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40341 mRNA. Translation: AAB67843.1.
AC008030 Genomic DNA. Translation: AAG10606.1.
CP002684 Genomic DNA. Translation: AEE31148.1.
AF367268 mRNA. Translation: AAK56257.1.
AY133548 mRNA. Translation: AAM91378.1.
PIRiF86422.
RefSeqiNP_564338.1. NM_102730.1.
UniGeneiAt.10370.

3D structure databases

ProteinModelPortaliQ42601.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25103. 2 interactions.
IntActiQ42601. 1 interaction.
STRINGi3702.AT1G29900.1.

PTM databases

iPTMnetiQ42601.

Proteomic databases

PaxDbiQ42601.
PRIDEiQ42601.
ProMEXiQ42601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G29900.1; AT1G29900.1; AT1G29900.
GeneIDi839868.
GrameneiAT1G29900.1; AT1G29900.1; AT1G29900.
KEGGiath:AT1G29900.

Organism-specific databases

TAIRiAT1G29900.

Phylogenomic databases

eggNOGiKOG0370. Eukaryota.
COG0458. LUCA.
HOGENOMiHOG000234582.
InParanoidiQ42601.
KOiK01955.
OMAiCCHTSFA.
OrthoDBiEOG093600HH.
PhylomeDBiQ42601.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.
BioCyciARA:AT1G29900-MONOMER.
ReactomeiR-ATH-500753. Pyrimidine biosynthesis.
R-ATH-70635. Urea cycle.

Miscellaneous databases

PROiQ42601.

Gene expression databases

GenevisibleiQ42601. AT.

Family and domain databases

Gene3Di1.10.1030.10. 1 hit.
3.30.1490.20. 2 hits.
3.30.470.20. 2 hits.
3.40.50.1380. 1 hit.
3.40.50.20. 2 hits.
HAMAPiMF_01210_A. CPSase_L_chain_A. 1 hit.
MF_01210_B. CPSase_L_chain_B. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006275. CarbamoylP_synth_lsu.
IPR005480. CarbamoylP_synth_lsu_oligo.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR005483. CbamoylP_synth_lsu_CPSase_dom.
IPR011607. MGS-like_dom.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamiPF02786. CPSase_L_D2. 2 hits.
PF02787. CPSase_L_D3. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PRINTSiPR00098. CPSASE.
SMARTiSM01096. CPSase_L_D3. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMiSSF48108. SSF48108. 1 hit.
SSF52335. SSF52335. 1 hit.
SSF52440. SSF52440. 2 hits.
TIGRFAMsiTIGR01369. CPSaseII_lrg. 1 hit.
PROSITEiPS50975. ATP_GRASP. 2 hits.
PS00866. CPSASE_1. 2 hits.
PS00867. CPSASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCARB_ARATH
AccessioniPrimary (citable) accession number: Q42601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 24, 2013
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The ven3-1, ven3-2, ven3-3 and ven3-4 phenotypes are rescued by exogenous application of citrulline, an arginine precursor.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.