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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial

Gene

At1g79010

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi123Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi126Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi129Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi133Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi165Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi168Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi172Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT1G79010-MONOMER
MetaCyc:AT1G79010-MONOMER
ReactomeiR-ATH-6799198 Complex I biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Ordered Locus Names:At1g79010
ORF Names:YUP8H12R.37, YUP8H12R_21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G79010
TAIRilocus:2207285 AT1G79010

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020016? – 222NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PaxDbiQ42599

PTM databases

SwissPalmiQ42599

Expressioni

Gene expression databases

ExpressionAtlasiQ42599 baseline and differential
GenevisibleiQ42599 AT

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

BioGridi29461, 1 interactor
IntActiQ42599, 2 interactors
STRINGi3702.AT1G79010.1

Structurei

3D structure databases

ProteinModelPortaliQ42599
SMRiQ42599
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 1434Fe-4S ferredoxin-type 1Add BLAST30
Domaini153 – 1824Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256 Eukaryota
COG1143 LUCA
HOGENOMiHOG000228289
InParanoidiQ42599
KOiK03941
OMAiEIARGMY
OrthoDBiEOG09360O5Z
PhylomeDBiQ42599

Family and domain databases

HAMAPiMF_01351 NDH1_NuoI, 1 hit
InterProiView protein in InterPro
IPR017896 4Fe4S_Fe-S-bd
IPR017900 4Fe4S_Fe_S_CS
IPR010226 NADH_quinone_OxRdtase_chainI
PfamiView protein in Pfam
PF12838 Fer4_7, 1 hit
TIGRFAMsiTIGR01971 NuoI, 1 hit
PROSITEiView protein in PROSITE
PS00198 4FE4S_FER_1, 2 hits
PS51379 4FE4S_FER_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILARRSL NTLRARHLVL SGQALQGSHL SRLQSRGISY GSNKDDEEAE
60 70 80 90 100
QLSKEISKDW NTVFERSINT LFLTEMVRGL SLTLKYFFDP KVTINYPFEK
110 120 130 140 150
GPLSPRFRGE HALRRYPTGE ERCIACKLCE AVCPAQAITI EAEEREDGSR
160 170 180 190 200
RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL
210 220
LENGDRWETE IAENLRSESL YR
Length:222
Mass (Da):25,503
Last modified:November 1, 1996 - v1
Checksum:i7C1F2264B0D0631B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84318 mRNA Translation: CAA59061.1
AC002986 Genomic DNA Translation: AAC17054.1
CP002684 Genomic DNA Translation: AEE36193.1
AY048241 mRNA Translation: AAK82503.1
AY072622 mRNA Translation: AAL62013.1
AK226368 mRNA Translation: BAE98516.1
AY088308 mRNA Translation: AAM65847.1
PIRiS52380
RefSeqiNP_178022.1, NM_106551.4
UniGeneiAt.24660

Genome annotation databases

EnsemblPlantsiAT1G79010.1; AT1G79010.1; AT1G79010
GeneIDi844242
GrameneiAT1G79010.1; AT1G79010.1; AT1G79010
KEGGiath:AT1G79010

Similar proteinsi

Entry informationi

Entry nameiNDS8A_ARATH
AccessioniPrimary (citable) accession number: Q42599
Secondary accession number(s): Q0WWI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health