Reviewed,
UniProtKB/Swiss-Prot Q42599 (NDUS8_ARATH)
Last modified
February 9, 2010.
Version 89.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial EC=1.6.5.3 EC=1.6.99.3 Alternative name(s): NADH-ubiquinone oxidoreductase 23 kDa subunit Complex I-23kD Short name=CI-23kD Complex I-28.5kD Short name=CI-28.5kD | ||||||
| Gene names |
| ||||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) [Complete proteome] | ||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis |
Protein attributes
| Sequence length | 222 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. May donate electrons to ubiquinone. |
| Catalytic activity | NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor. |
| Cofactor | Binds 2 4Fe-4S clusters per subunit By similarity. |
| Subunit structure | Complex I is composed of about 40 different subunits. |
| Subcellular location | Mitochondrion Probable Ref.5. |
| Sequence similarities | Belongs to the complex I 23 kDa subunit family. Contains 2 4Fe-4S ferredoxin-type domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Electron transport Respiratory chain Transport |
| Cellular component | Mitochondrion |
| Domain | Repeat Transit peptide |
| Ligand | 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | mitochondrion Ref.5 Inferred from direct assay. Source: TAIR respiratory chain complex IInferred from direct assay. Source: TAIR |
| Molecular function | 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activityInferred from electronic annotation. Source: EC electron carrier activityInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – ? | Mitochondrion By similarity | |||||||
| Chain | ? – 222 | NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial | PRO_0000020016 | ||||||
Regions | |||||||||
| Domain | 114 – 143 | 30 | 4Fe-4S ferredoxin-type 1 | ||||||
| Domain | 153 – 182 | 30 | 4Fe-4S ferredoxin-type 2 | ||||||
Sites | |||||||||
| Metal binding | 123 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 126 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 129 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
| Metal binding | 133 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 162 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 165 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 168 | 1 | Iron-sulfur 2 (4Fe-4S) By similarity | ||||||
| Metal binding | 172 | 1 | Iron-sulfur 1 (4Fe-4S) By similarity | ||||||
Sequences
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References
| [1] | "The 28.5-kDa iron-sulfur protein of mitochondrial complex I is encoded in the nucleus in plants." Schmidt-Bleek K., Heiser V., Thieck O., Brennicke A., Grohmann L. Mol. Gen. Genet. 253:448-454(1997) [PubMed: 9037104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. C24. |
| [2] | "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. Davis R.W.Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [4] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [5] | "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X84318 mRNA. Translation: CAA59061.1. AC002986 Genomic DNA. Translation: AAC17054.1. AY048241 mRNA. Translation: AAK82503.1. AY072622 mRNA. Translation: AAL62013.1. AY088308 mRNA. Translation: AAM65847.1. |
| IPI | IPI00539763. |
| PIR | S52380. |
| RefSeq | NP_178022.1. |
| UniGene | At.24660 Rra.366 Rsa.9070 |
3D structure databases | |
| SMR | Q42599. Positions 96-212. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q42599. |
Proteomic databases | |
| PRIDE | Q42599. |
Genome annotation databases | |
| GeneID | 844242. |
| GenomeReviews | Gene locus AT1G79010 in contig CT485782_GR. |
| KEGG | ath:AT1G79010. |
| NMPDR | fig|3702.1.peg.7463. |
Organism-specific databases | |
| GeneFarm | 1821. 140. |
| TAIR | At1g79010. |
Phylogenomic databases | |
| HOGENOM | HBG724359. |
| InParanoid | Q42599. |
| OMA | EMVRGLM. |
| PhylomeDB | Q42599. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:AT1G79010-MONOMER. |
| BRENDA | 1.6.5.3. 302. 1.6.99.3. 302. |
Gene expression databases | |
| ArrayExpress | Q42599. |
| Genevestigator | Q42599. |
| GermOnline | AT1G79010. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR017896. 4Fe4S_Fe-S-bd. IPR017900. 4Fe4S_Fe_S_CS. IPR009051. Helical_ferredxn. IPR010226. NADH_quinone_OxRdtase_chainI. [Graphical view] |
| TIGRFAMs | TIGR01971. NuoI. 1 hit. |
| PROSITE | PS00198. 4FE4S_FER_1. 2 hits. PS51379. 4FE4S_FER_2. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NDUS8_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q42599 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with


