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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial

Gene

At1g79010

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi123Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi126Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi129Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi133Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi165Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi168Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi172Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:AT1G79010-MONOMER.
MetaCyc:AT1G79010-MONOMER.
ReactomeiR-ATH-6799198. Complex I biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Gene namesi
Ordered Locus Names:At1g79010
ORF Names:YUP8H12R.37, YUP8H12R_21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G79010.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial respiratory chain complex I Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020016? – 222NADH dehydrogenase [ubiquinone] iron-sulfur protein 8-A, mitochondrial
Transit peptidei1 – ?MitochondrionBy similarity

Proteomic databases

PaxDbiQ42599.

PTM databases

SwissPalmiQ42599.

Expressioni

Gene expression databases

GenevisibleiQ42599. AT.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

BioGridi29461. 1 interactor.
IntActiQ42599. 2 interactors.
STRINGi3702.AT1G79010.1.

Structurei

3D structure databases

ProteinModelPortaliQ42599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 1434Fe-4S ferredoxin-type 1Add BLAST30
Domaini153 – 1824Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
HOGENOMiHOG000228289.
InParanoidiQ42599.
KOiK03941.
OMAiANAINIK.
OrthoDBiEOG09360O5Z.
PhylomeDBiQ42599.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42599-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILARRSL NTLRARHLVL SGQALQGSHL SRLQSRGISY GSNKDDEEAE
60 70 80 90 100
QLSKEISKDW NTVFERSINT LFLTEMVRGL SLTLKYFFDP KVTINYPFEK
110 120 130 140 150
GPLSPRFRGE HALRRYPTGE ERCIACKLCE AVCPAQAITI EAEEREDGSR
160 170 180 190 200
RTTRYDIDMT KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL
210 220
LENGDRWETE IAENLRSESL YR
Length:222
Mass (Da):25,503
Last modified:November 1, 1996 - v1
Checksum:i7C1F2264B0D0631B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84318 mRNA. Translation: CAA59061.1.
AC002986 Genomic DNA. Translation: AAC17054.1.
CP002684 Genomic DNA. Translation: AEE36193.1.
AY048241 mRNA. Translation: AAK82503.1.
AY072622 mRNA. Translation: AAL62013.1.
AK226368 mRNA. Translation: BAE98516.1.
AY088308 mRNA. Translation: AAM65847.1.
PIRiS52380.
RefSeqiNP_178022.1. NM_106551.4.
UniGeneiAt.24660.

Genome annotation databases

EnsemblPlantsiAT1G79010.1; AT1G79010.1; AT1G79010.
GeneIDi844242.
GrameneiAT1G79010.1; AT1G79010.1; AT1G79010.
KEGGiath:AT1G79010.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84318 mRNA. Translation: CAA59061.1.
AC002986 Genomic DNA. Translation: AAC17054.1.
CP002684 Genomic DNA. Translation: AEE36193.1.
AY048241 mRNA. Translation: AAK82503.1.
AY072622 mRNA. Translation: AAL62013.1.
AK226368 mRNA. Translation: BAE98516.1.
AY088308 mRNA. Translation: AAM65847.1.
PIRiS52380.
RefSeqiNP_178022.1. NM_106551.4.
UniGeneiAt.24660.

3D structure databases

ProteinModelPortaliQ42599.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29461. 1 interactor.
IntActiQ42599. 2 interactors.
STRINGi3702.AT1G79010.1.

PTM databases

SwissPalmiQ42599.

Proteomic databases

PaxDbiQ42599.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G79010.1; AT1G79010.1; AT1G79010.
GeneIDi844242.
GrameneiAT1G79010.1; AT1G79010.1; AT1G79010.
KEGGiath:AT1G79010.

Organism-specific databases

TAIRiAT1G79010.

Phylogenomic databases

eggNOGiKOG3256. Eukaryota.
COG1143. LUCA.
HOGENOMiHOG000228289.
InParanoidiQ42599.
KOiK03941.
OMAiANAINIK.
OrthoDBiEOG09360O5Z.
PhylomeDBiQ42599.

Enzyme and pathway databases

BioCyciARA:AT1G79010-MONOMER.
MetaCyc:AT1G79010-MONOMER.
ReactomeiR-ATH-6799198. Complex I biogenesis.

Miscellaneous databases

PROiQ42599.

Gene expression databases

GenevisibleiQ42599. AT.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDS8A_ARATH
AccessioniPrimary (citable) accession number: Q42599
Secondary accession number(s): Q0WWI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.