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Protein

L-ascorbate peroxidase T, chloroplastic

Gene

APXT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei108Transition state stabilizerPROSITE-ProRule annotation1
Active sitei112Proton acceptorPROSITE-ProRule annotation1
Metal bindingi241Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi242Potassium or calciumBy similarity1
Metal bindingi274Potassium or calciumBy similarity1
Metal bindingi281Potassium or calciumBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • chloroplast-nucleus signaling pathway Source: TAIR
  • cold acclimation Source: TAIR
  • hydrogen peroxide catabolic process Source: GO_Central
  • hydrogen peroxide mediated signaling pathway Source: TAIR
  • response to reactive oxygen species Source: GO_Central

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT1G77490-MONOMER
BRENDAi1.11.1.11 399

Protein family/group databases

PeroxiBasei1889 AtAPx06

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase T, chloroplastic (EC:1.11.1.11)
Alternative name(s):
Thylakoid-bound ascorbate peroxidase
Short name:
AtAPx06
Short name:
tAPX
Gene namesi
Name:APXT
Ordered Locus Names:At1g77490
ORF Names:T5M16.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G77490
TAIRilocus:2204735 AT1G77490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei397 – 417HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei? – 78ThylakoidSequence analysis
Transit peptidei1 – ?ChloroplastSequence analysis
ChainiPRO_000026132779 – 426L-ascorbate peroxidase T, chloroplasticAdd BLAST348

Proteomic databases

PaxDbiQ42593
PRIDEiQ42593

PTM databases

iPTMnetiQ42593

Expressioni

Developmental stagei

Down-regulated during leaf senescence.1 Publication

Gene expression databases

ExpressionAtlasiQ42593 differential
GenevisibleiQ42593 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G77490.1

Structurei

3D structure databases

ProteinModelPortaliQ42593
SMRiQ42593
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG24 Eukaryota
COG0376 LUCA
HOGENOMiHOG000189824
InParanoidiQ42593
KOiK00434
OMAiTNRGSSN
OrthoDBiEOG09360BRN
PhylomeDBiQ42593

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSLSAASH LLCSSTRVSL SPAVTSSSSS PVVALSSSTS PHSLGSVASS
60 70 80 90 100
SLFPHSSFVL QKKHPINGTS TRMISPKCAA SDAAQLISAK EDIKVLLRTK
110 120 130 140 150
FCHPILVRLG WHDAGTYNKN IEEWPLRGGA NGSLRFEAEL KHAANAGLLN
160 170 180 190 200
ALKLIQPLKD KYPNISYADL FQLASATAIE EAGGPDIPMK YGRVDVVAPE
210 220 230 240 250
QCPEEGRLPD AGPPSPADHL RDVFYRMGLD DKEIVALSGA HTLGRARPDR
260 270 280 290 300
SGWGKPETKY TKTGPGEAGG QSWTVKWLKF DNSYFKDIKE KRDDDLLVLP
310 320 330 340 350
TDAALFEDPS FKNYAEKYAE DVAAFFKDYA EAHAKLSNLG AKFDPPEGIV
360 370 380 390 400
IENVPEKFVA AKYSTGKKEL SDSMKKKIRA EYEAIGGSPD KPLPTNYFLN
410 420
IIIAIGVLVL LSTLFGGNNN SDFSGF
Length:426
Mass (Da):46,092
Last modified:November 28, 2006 - v2
Checksum:i9205E8E77B73BBAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73M → K in CAA67426 (PubMed:9291097).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98926 mRNA Translation: CAA67426.1
AC010704 Genomic DNA Translation: AAG51660.1
CP002684 Genomic DNA Translation: AEE35985.1
AK229693 mRNA Translation: BAF01533.1
AY085554 mRNA Translation: AAM62777.1
PIRiC96804
RefSeqiNP_177873.1, NM_106398.3
UniGeneiAt.25463

Genome annotation databases

EnsemblPlantsiAT1G77490.1; AT1G77490.1; AT1G77490
GeneIDi844085
GrameneiAT1G77490.1; AT1G77490.1; AT1G77490
KEGGiath:AT1G77490

Similar proteinsi

Entry informationi

Entry nameiAPXT_ARATH
AccessioniPrimary (citable) accession number: Q42593
Secondary accession number(s): Q9CAQ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: May 23, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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