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Protein

L-ascorbate peroxidase T, chloroplastic

Gene

APXT

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei108 – 1081Transition state stabilizerPROSITE-ProRule annotation
Active sitei112 – 1121Proton acceptorPROSITE-ProRule annotation
Metal bindingi241 – 2411Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi242 – 2421Potassium or calciumBy similarity
Metal bindingi274 – 2741Potassium or calciumBy similarity
Metal bindingi281 – 2811Potassium or calciumBy similarity

GO - Molecular functioni

GO - Biological processi

  • chloroplast-nucleus signaling pathway Source: TAIR
  • cold acclimation Source: TAIR
  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • hydrogen peroxide mediated signaling pathway Source: TAIR
  • response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT1G77490-MONOMER.
BRENDAi1.11.1.11. 399.

Protein family/group databases

PeroxiBasei1889. AtAPx06.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase T, chloroplastic (EC:1.11.1.11)
Alternative name(s):
Thylakoid-bound ascorbate peroxidase
Short name:
AtAPx06
Short name:
tAPX
Gene namesi
Name:APXT
Ordered Locus Names:At1g77490
ORF Names:T5M16.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G77490.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei397 – 41721HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei? – 78ThylakoidSequence analysis
Transit peptidei1 – ?ChloroplastSequence analysis
Chaini79 – 426348L-ascorbate peroxidase T, chloroplasticPRO_0000261327Add
BLAST

Proteomic databases

PaxDbiQ42593.
PRIDEiQ42593.

PTM databases

iPTMnetiQ42593.

Expressioni

Developmental stagei

Down-regulated during leaf senescence.1 Publication

Gene expression databases

GenevisibleiQ42593. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G77490.1.

Structurei

3D structure databases

ProteinModelPortaliQ42593.
SMRiQ42593. Positions 79-352.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG24. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42593.
KOiK00434.
OMAiLRTKFCH.
PhylomeDBiQ42593.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42593-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSLSAASH LLCSSTRVSL SPAVTSSSSS PVVALSSSTS PHSLGSVASS
60 70 80 90 100
SLFPHSSFVL QKKHPINGTS TRMISPKCAA SDAAQLISAK EDIKVLLRTK
110 120 130 140 150
FCHPILVRLG WHDAGTYNKN IEEWPLRGGA NGSLRFEAEL KHAANAGLLN
160 170 180 190 200
ALKLIQPLKD KYPNISYADL FQLASATAIE EAGGPDIPMK YGRVDVVAPE
210 220 230 240 250
QCPEEGRLPD AGPPSPADHL RDVFYRMGLD DKEIVALSGA HTLGRARPDR
260 270 280 290 300
SGWGKPETKY TKTGPGEAGG QSWTVKWLKF DNSYFKDIKE KRDDDLLVLP
310 320 330 340 350
TDAALFEDPS FKNYAEKYAE DVAAFFKDYA EAHAKLSNLG AKFDPPEGIV
360 370 380 390 400
IENVPEKFVA AKYSTGKKEL SDSMKKKIRA EYEAIGGSPD KPLPTNYFLN
410 420
IIIAIGVLVL LSTLFGGNNN SDFSGF
Length:426
Mass (Da):46,092
Last modified:November 28, 2006 - v2
Checksum:i9205E8E77B73BBAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731M → K in CAA67426 (PubMed:9291097).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98926 mRNA. Translation: CAA67426.1.
AC010704 Genomic DNA. Translation: AAG51660.1.
CP002684 Genomic DNA. Translation: AEE35985.1.
AK229693 mRNA. Translation: BAF01533.1.
AY085554 mRNA. Translation: AAM62777.1.
PIRiC96804.
RefSeqiNP_177873.1. NM_106398.2.
UniGeneiAt.25463.

Genome annotation databases

EnsemblPlantsiAT1G77490.1; AT1G77490.1; AT1G77490.
GeneIDi844085.
GrameneiAT1G77490.1; AT1G77490.1; AT1G77490.
KEGGiath:AT1G77490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98926 mRNA. Translation: CAA67426.1.
AC010704 Genomic DNA. Translation: AAG51660.1.
CP002684 Genomic DNA. Translation: AEE35985.1.
AK229693 mRNA. Translation: BAF01533.1.
AY085554 mRNA. Translation: AAM62777.1.
PIRiC96804.
RefSeqiNP_177873.1. NM_106398.2.
UniGeneiAt.25463.

3D structure databases

ProteinModelPortaliQ42593.
SMRiQ42593. Positions 79-352.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G77490.1.

Protein family/group databases

PeroxiBasei1889. AtAPx06.

PTM databases

iPTMnetiQ42593.

Proteomic databases

PaxDbiQ42593.
PRIDEiQ42593.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G77490.1; AT1G77490.1; AT1G77490.
GeneIDi844085.
GrameneiAT1G77490.1; AT1G77490.1; AT1G77490.
KEGGiath:AT1G77490.

Organism-specific databases

TAIRiAT1G77490.

Phylogenomic databases

eggNOGiENOG410IG24. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42593.
KOiK00434.
OMAiLRTKFCH.
PhylomeDBiQ42593.

Enzyme and pathway databases

BioCyciARA:AT1G77490-MONOMER.
BRENDAi1.11.1.11. 399.

Miscellaneous databases

PROiQ42593.

Gene expression databases

GenevisibleiQ42593. AT.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase."
    Jespersen H.M., Kjaersgaard I.V.H., Oestergaard L., Welinder K.G.
    Biochem. J. 326:305-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Expression of the Apx gene family during leaf senescence of Arabidopsis thaliana."
    Panchuk I.I., Zentgraf U., Volkov R.A.
    Planta 222:926-932(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiAPXT_ARATH
AccessioniPrimary (citable) accession number: Q42593
Secondary accession number(s): Q9CAQ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: June 8, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.