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Protein

L-ascorbate peroxidase S, chloroplastic/mitochondrial

Gene

APXS

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1291Transition state stabilizerPROSITE-ProRule annotation
Active sitei133 – 1331Proton acceptorPROSITE-ProRule annotation
Metal bindingi262 – 2621Iron (heme axial ligand)PROSITE-ProRule annotation
Metal bindingi263 – 2631Potassium or calciumBy similarity
Metal bindingi295 – 2951Potassium or calciumBy similarity
Metal bindingi302 – 3021Potassium or calciumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT4G08390-MONOMER.
ARA:GQT-1710-MONOMER.
ARA:GQT-1711-MONOMER.
ARA:GQT-1712-MONOMER.
BRENDAi1.11.1.11. 399.

Protein family/group databases

PeroxiBasei1886. AtAPx05.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase S, chloroplastic/mitochondrial (EC:1.11.1.11)
Alternative name(s):
Stromal ascorbate peroxidase
Short name:
AtAPx05
Short name:
sAPX
Gene namesi
Name:APXS
Ordered Locus Names:At4g08390
ORF Names:T28D5.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G08390.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 372L-ascorbate peroxidase S, chloroplastic/mitochondrialPRO_0000261326
Transit peptidei1 – ?Chloroplast and mitochondrionSequence analysis

Proteomic databases

PaxDbiQ42592.
PRIDEiQ42592.

PTM databases

iPTMnetiQ42592.

Expressioni

Gene expression databases

ExpressionAtlasiQ42592. baseline and differential.
GenevisibleiQ42592. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G08390.1.

Structurei

3D structure databases

ProteinModelPortaliQ42592.
SMRiQ42592. Positions 100-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IG24. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42592.
KOiK00434.
OMAiDYTESHA.
PhylomeDBiQ42592.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42592-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAERVSLTLN GTLLSPPPTT TTTTMSSSLR STTAASLLLR SSSSSSRSTL
60 70 80 90 100
TLSASSSLSF VRSLVSSPRL SSSSSLSQKK YRIASVNRSF NSTTAATKSS
110 120 130 140 150
SSDPDQLKNA REDIKELLST KFCHPILVRL GWHDAGTYNK NIKEWPQRGG
160 170 180 190 200
ANGSLRFDIE LKHAANAGLV NALNLIKDIK EKYSGISYAD LFQLASATAI
210 220 230 240 250
EEAGGPKIPM KYGRVDASGP EDCPEEGRLP DAGPPSPATH LREVFYRMGL
260 270 280 290 300
DDKDIVALSG AHTLGRSRPE RSGWGKPETK YTKEGPGAPG GQSWTPEWLK
310 320 330 340 350
FDNSYFKEIK EKRDEDLLVL PTDAAIFEDS SFKVYAEKYA ADQDAFFKDY
360 370
AVAHAKLSNL GAEFNPPEGI VI
Length:372
Mass (Da):40,407
Last modified:November 28, 2006 - v2
Checksum:iA5D94B9A20720B87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111G → V in CAA67425 (PubMed:9291097).Curated
Sequence conflicti75 – 751S → P in CAA67425 (PubMed:9291097).Curated
Sequence conflicti81 – 811Y → C in CAA67425 (PubMed:9291097).Curated
Sequence conflicti371 – 3711V → I in CAA67425 (PubMed:9291097).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98925 mRNA. Translation: CAA67425.1.
AL109819 Genomic DNA. Translation: CAB52561.1.
AL161511 Genomic DNA. Translation: CAB77964.1.
CP002687 Genomic DNA. Translation: AEE82634.1.
CP002687 Genomic DNA. Translation: AEE82635.1.
AY056319 mRNA. Translation: AAL07168.1.
AY114065 mRNA. Translation: AAM45113.1.
PIRiT14193.
RefSeqiNP_192579.1. NM_116908.2. [Q42592-1]
NP_974520.1. NM_202791.3. [Q42592-1]
UniGeneiAt.22866.

Genome annotation databases

EnsemblPlantsiAT4G08390.1; AT4G08390.1; AT4G08390. [Q42592-1]
AT4G08390.2; AT4G08390.2; AT4G08390. [Q42592-1]
GeneIDi826396.
KEGGiath:AT4G08390.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98925 mRNA. Translation: CAA67425.1.
AL109819 Genomic DNA. Translation: CAB52561.1.
AL161511 Genomic DNA. Translation: CAB77964.1.
CP002687 Genomic DNA. Translation: AEE82634.1.
CP002687 Genomic DNA. Translation: AEE82635.1.
AY056319 mRNA. Translation: AAL07168.1.
AY114065 mRNA. Translation: AAM45113.1.
PIRiT14193.
RefSeqiNP_192579.1. NM_116908.2. [Q42592-1]
NP_974520.1. NM_202791.3. [Q42592-1]
UniGeneiAt.22866.

3D structure databases

ProteinModelPortaliQ42592.
SMRiQ42592. Positions 100-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G08390.1.

Protein family/group databases

PeroxiBasei1886. AtAPx05.

PTM databases

iPTMnetiQ42592.

Proteomic databases

PaxDbiQ42592.
PRIDEiQ42592.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G08390.1; AT4G08390.1; AT4G08390. [Q42592-1]
AT4G08390.2; AT4G08390.2; AT4G08390. [Q42592-1]
GeneIDi826396.
KEGGiath:AT4G08390.

Organism-specific databases

TAIRiAT4G08390.

Phylogenomic databases

eggNOGiENOG410IG24. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42592.
KOiK00434.
OMAiDYTESHA.
PhylomeDBiQ42592.

Enzyme and pathway databases

BioCyciARA:AT4G08390-MONOMER.
ARA:GQT-1710-MONOMER.
ARA:GQT-1711-MONOMER.
ARA:GQT-1712-MONOMER.
BRENDAi1.11.1.11. 399.

Miscellaneous databases

PROiQ42592.

Gene expression databases

ExpressionAtlasiQ42592. baseline and differential.
GenevisibleiQ42592. AT.

Family and domain databases

InterProiIPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase."
    Jespersen H.M., Kjaersgaard I.V.H., Oestergaard L., Welinder K.G.
    Biochem. J. 326:305-310(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Molecular definition of the ascorbate-glutathione cycle in Arabidopsis mitochondria reveals dual targeting of antioxidant defenses in plants."
    Chew O., Whelan J., Millar A.H.
    J. Biol. Chem. 278:46869-46877(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAPXS_ARATH
AccessioniPrimary (citable) accession number: Q42592
Secondary accession number(s): Q9STM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 28, 2006
Last modified: June 8, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.