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Protein

Serine acetyltransferase 1, chloroplastic

Gene

SAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in detoxification process by mediating the production of glutathione.

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Kineticsi

  1. KM=1.64 mM for L-Ser (at pH 8 and 37 degrees Celsius)1 Publication
  2. KM=0.16 mM for acetyl-CoA (at pH 8 and 37 degrees Celsius)1 Publication

    Pathwayi: L-cysteine biosynthesis

    This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
    Proteins known to be involved in the 2 steps of the subpathway in this organism are:
    1. Serine acetyltransferase 3, mitochondrial (SAT3), Serine acetyltransferase 5 (SAT5), Serine acetyltransferase 1, chloroplastic (SAT1), Serine acetyltransferase 2 (SAT2), Serine acetyltransferase 4 (SAT4)
    2. Bifunctional L-3-cyanoalanine synthase/cysteine synthase D2 (CYSD2), Bifunctional cystathionine gamma-lyase/cysteine synthase (DES1), Cysteine synthase, mitochondrial (OASC), Bifunctional L-3-cyanoalanine synthase/cysteine synthase D1 (CYSD1), Cysteine synthase, chloroplastic/chromoplastic (OASB), Cysteine synthase 1 (OASA1)
    This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

    GO - Molecular functioni

    • serine O-acetyltransferase activity Source: TAIR

    GO - Biological processi

    • cellular response to sulfate starvation Source: TAIR
    • cysteine biosynthetic process Source: GO_Central
    • cysteine biosynthetic process from serine Source: InterPro
    • response to cold Source: TAIR

    Keywordsi

    Molecular functionAcyltransferase, Transferase
    Biological processAmino-acid biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT1G55920-MONOMER
    MetaCyc:AT1G55920-MONOMER
    BRENDAi2.3.1.30 399
    SABIO-RKQ42588
    UniPathwayiUPA00136; UER00199

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine acetyltransferase 1, chloroplastic (EC:2.3.1.30)
    Short name:
    AtSAT-1
    Alternative name(s):
    AtSERAT2;1
    SAT-p
    Gene namesi
    Name:SAT1
    Synonyms:SAT5
    Ordered Locus Names:At1g55920
    ORF Names:F14J16.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G55920
    TAIRilocus:2012085 AT1G55920

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Chloroplast, Cytoplasm, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000686901 – 314Serine acetyltransferase 1, chloroplasticAdd BLAST314

    Proteomic databases

    PaxDbiQ42588
    PRIDEiQ42588

    Expressioni

    Tissue specificityi

    Mostly expressed in leaves. Localized in cortex, trichomes and vascular tissues, particularly in phloem.4 Publications

    Inductioni

    By cadmium (Cd). Not induced under sulfur-deficient conditions. Repressed in trichomes in response to NaCl treatment.3 Publications

    Gene expression databases

    ExpressionAtlasiQ42588 differential
    GenevisibleiQ42588 AT

    Interactioni

    Subunit structurei

    Homomultimer (By similarity). Interacts with OASA1 and CYP20-3. Component of the cysteine synthase complex (CSC) composed of two OAS-TL dimers and one SAT hexamer.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CYP20-3P347913EBI-1633480,EBI-449385

    Protein-protein interaction databases

    BioGridi27268, 2 interactors
    DIPiDIP-40647N
    IntActiQ42588, 2 interactors
    STRINGi3702.AT1G55920.1

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2ISQX-ray2.80B307-314[»]
    ProteinModelPortaliQ42588
    SMRiQ42588
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ42588

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG410INAF Eukaryota
    COG1045 LUCA
    HOGENOMiHOG000049437
    InParanoidiQ42588
    KOiK00640
    OMAiYASITSH
    OrthoDBiEOG09360EFX
    PhylomeDBiQ42588

    Family and domain databases

    InterProiView protein in InterPro
    IPR001451 Hexapep
    IPR018357 Hexapep_transf_CS
    IPR010493 Ser_AcTrfase_N
    IPR005881 Ser_O-AcTrfase
    IPR011004 Trimer_LpxA-like_sf
    PfamiView protein in Pfam
    PF00132 Hexapep, 2 hits
    PF06426 SATase_N, 1 hit
    PIRSFiPIRSF000441 CysE, 1 hit
    SMARTiView protein in SMART
    SM00971 SATase_N, 1 hit
    SUPFAMiSSF51161 SSF51161, 1 hit
    TIGRFAMsiTIGR01172 cysE, 1 hit
    PROSITEiView protein in PROSITE
    PS00101 HEXAPEP_TRANSFERASES, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q42588-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATCIDTCRT GNTQDDDSRF CCIKNFFRPG FSVNRKIHHT QIEDDDDVWI
    60 70 80 90 100
    KMLEEAKSDV KQEPILSNYY YASITSHRSL ESALAHILSV KLSNLNLPSN
    110 120 130 140 150
    TLFELFISVL EESPEIIEST KQDLIAVKER DPACISYVHC FLGFKGFLAC
    160 170 180 190 200
    QAHRIAHTLW KQNRKIVALL IQNRVSESFA VDIHPGAKIG KGILLDHATG
    210 220 230 240 250
    VVIGETAVVG DNVSILHGVT LGGTGKQSGD RHPKIGDGVL IGAGSCILGN
    260 270 280 290 300
    ITIGEGAKIG SGSVVVKDVP ARTTAVGNPA RLIGGKENPR KHDKIPCLTM
    310
    DQTSYLTEWS DYVI
    Length:314
    Mass (Da):34,251
    Last modified:January 24, 2006 - v2
    Checksum:i78FACE3DA5CE04B0
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti6D → H in AAC37474 (PubMed:8867790).Curated1
    Sequence conflicti24 – 25KN → NK in AAC37474 (PubMed:8867790).Curated2
    Sequence conflicti54E → K in AAC37474 (PubMed:8867790).Curated1
    Sequence conflicti57K → E in AAC37474 (PubMed:8867790).Curated1
    Sequence conflicti85A → G in AAC37474 (PubMed:8867790).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L42212 mRNA Translation: AAC37474.1
    L34076 Genomic RNA Translation: AAA58608.1
    Z34888 Genomic DNA Translation: CAA84371.1
    AC002304 Genomic DNA Translation: AAF79319.1
    CP002684 Genomic DNA Translation: AEE33320.1
    BT008309 mRNA Translation: AAP37668.1
    AK227691 mRNA Translation: BAE99678.1
    PIRiS67482
    S71181
    RefSeqiNP_175988.1, NM_104470.3
    UniGeneiAt.24052

    Genome annotation databases

    EnsemblPlantsiAT1G55920.1; AT1G55920.1; AT1G55920
    GeneIDi842043
    GrameneiAT1G55920.1; AT1G55920.1; AT1G55920
    KEGGiath:AT1G55920

    Similar proteinsi

    Entry informationi

    Entry nameiSAT1_ARATH
    AccessioniPrimary (citable) accession number: Q42588
    Secondary accession number(s): Q0WT70, Q43297
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: January 24, 2006
    Last modified: May 23, 2018
    This is version 125 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

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