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Q42586

- UMPS_ARATH

UniProt

Q42586 - UMPS_ARATH

Protein

Uridine 5'-monophosphate synthase

Gene

PYRE-F

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (06 Jun 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
    Orotidine 5'-phosphate = UMP + CO2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei305 – 3051By similarity

    GO - Molecular functioni

    1. orotate phosphoribosyltransferase activity Source: TAIR
    2. orotidine-5'-phosphate decarboxylase activity Source: TAIR

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    3. cellular response to phosphate starvation Source: TAIR
    4. pyrimidine ribonucleotide biosynthetic process Source: TAIR
    5. response to cadmium ion Source: TAIR

    Keywords - Molecular functioni

    Decarboxylase, Glycosyltransferase, Lyase, Transferase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT3G54470-MONOMER.
    MetaCyc:AT3G54470-MONOMER.
    UniPathwayiUPA00070; UER00119.
    UPA00070; UER00120.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uridine 5'-monophosphate synthase
    Short name:
    UMP synthase
    Including the following 2 domains:
    Orotate phosphoribosyltransferase (EC:2.4.2.10)
    Short name:
    OPRTase
    Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
    Alternative name(s):
    OMPdecase
    Gene namesi
    Name:PYRE-F
    Synonyms:UMPS
    Ordered Locus Names:At3g54470
    ORF Names:T14E10.40
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G54470.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476Uridine 5'-monophosphate synthasePRO_0000139653Add
    BLAST

    Proteomic databases

    PaxDbiQ42586.
    PRIDEiQ42586.

    Expressioni

    Gene expression databases

    GenevestigatoriQ42586.

    Interactioni

    Protein-protein interaction databases

    BioGridi9928. 1 interaction.
    STRINGi3702.AT3G54470.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42586.
    SMRiQ42586. Positions 7-198, 217-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    In the C-terminal section; belongs to the OMP decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0284.
    HOGENOMiHOG000213905.
    InParanoidiQ42586.
    KOiK13421.
    OMAiSMKPEFL.
    PhylomeDBiQ42586.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPiMF_01208. PyrE.
    InterProiIPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SMARTiSM00934. OMPdecase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR00336. pyrE. 1 hit.
    TIGR01740. pyrF. 1 hit.
    PROSITEiPS00156. OMPDECASE. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q42586-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAMEALILQ LHEIGAVKFG NFKLKSGIFS PVYIDLRLIV SYPSLLTQIS    50
    QTLISSLPPS ATFDVVCGVP YTALPIATVV SVSNGIPMLM RRKEIKDYGT 100
    SKAIEGIFEK DQTCLIIEDL VTSGASVLET AAPLRAVGLK VSDAVVLIDR 150
    QQGGRENLAE NGIKLHSMIM LTDMVRVLKE KGKIEVEVEV NLLKFLEENR 200
    RVSVPSVEKP KPKPRVLGFK ERSELSKNPT GKKLFDIMLK KETNLCLAAD 250
    VGTAAELLDI ADKVGPEICL LKTHVDILPD FTPDFGSKLR AIADKHKFLI 300
    FEDRKFADIG NTVTMQYEGG IFKILEWADI INAHVISGPG IVDGLKLKGM 350
    PRGRGLLLLA EMSSAGNLAT GDYTAAAVKI ADAHSDFVMG FISVNPASWK 400
    CGYVYPSMIH ATPGVQMVKG GDALGQQYNT PHSVITERGS DIIIVGRGII 450
    KAENPAETAH EYRVQGWNAY LEKCSQ 476
    Length:476
    Mass (Da):51,850
    Last modified:June 6, 2002 - v2
    Checksum:iF79AE7992503D928
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti186 – 1861V → E(PubMed:8041358)Curated
    Sequence conflicti186 – 1861V → E1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71842 mRNA. Translation: CAA50686.1.
    AF276887 Genomic DNA. Translation: AAK69440.1.
    AL138656 Genomic DNA. Translation: CAB77567.1.
    CP002686 Genomic DNA. Translation: AEE79235.1.
    AY140098 mRNA. Translation: AAM98239.1.
    BT006612 mRNA. Translation: AAP31956.1.
    PIRiS46440.
    T47606.
    RefSeqiNP_680130.1. NM_148875.4.
    UniGeneiAt.108.

    Genome annotation databases

    EnsemblPlantsiAT3G54470.1; AT3G54470.1; AT3G54470.
    GeneIDi824612.
    KEGGiath:AT3G54470.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X71842 mRNA. Translation: CAA50686.1 .
    AF276887 Genomic DNA. Translation: AAK69440.1 .
    AL138656 Genomic DNA. Translation: CAB77567.1 .
    CP002686 Genomic DNA. Translation: AEE79235.1 .
    AY140098 mRNA. Translation: AAM98239.1 .
    BT006612 mRNA. Translation: AAP31956.1 .
    PIRi S46440.
    T47606.
    RefSeqi NP_680130.1. NM_148875.4.
    UniGenei At.108.

    3D structure databases

    ProteinModelPortali Q42586.
    SMRi Q42586. Positions 7-198, 217-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 9928. 1 interaction.
    STRINGi 3702.AT3G54470.1-P.

    Proteomic databases

    PaxDbi Q42586.
    PRIDEi Q42586.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G54470.1 ; AT3G54470.1 ; AT3G54470 .
    GeneIDi 824612.
    KEGGi ath:AT3G54470.

    Organism-specific databases

    TAIRi AT3G54470.

    Phylogenomic databases

    eggNOGi COG0284.
    HOGENOMi HOG000213905.
    InParanoidi Q42586.
    KOi K13421.
    OMAi SMKPEFL.
    PhylomeDBi Q42586.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00119 .
    UPA00070 ; UER00120 .
    BioCyci ARA:AT3G54470-MONOMER.
    MetaCyc:AT3G54470-MONOMER.

    Miscellaneous databases

    PROi Q42586.

    Gene expression databases

    Genevestigatori Q42586.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPi MF_01208. PyrE.
    InterProi IPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SMARTi SM00934. OMPdecase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR00336. pyrE. 1 hit.
    TIGR01740. pyrF. 1 hit.
    PROSITEi PS00156. OMPDECASE. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway."
      Nasr F., Bertauche N., Dufour M.E., Minet M., Lacroute F.
      Mol. Gen. Genet. 244:23-32(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Transcriptional analysis of the Arabidopsis thaliana UMP synthase locus."
      Kafer C.W., Thornburg R.W.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Columbia.
    3. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiUMPS_ARATH
    AccessioniPrimary (citable) accession number: Q42586
    Secondary accession number(s): Q9M1I0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: June 6, 2002
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3