Reviewed, UniProtKB/Swiss-Prot Q42586 (PYR5_ARATH)

Names and origin

Protein names

Recommended name:
    Uridine 5'-monophosphate synthase
      Short name=UMP synthase
Including the following 2 domains:
    1- Recommended name:
            Orotate phosphoribosyltransferase
                Short name=OPRTase
              EC=2.4.2.10
    2- Recommended name:
            Orotidine 5'-phosphate decarboxylase
              EC=4.1.1.23
        Alternative name(s):
            OMPdecase

Gene names

Name:	PYRE-F
Synonyms:	UMPS
Ordered Locus Names:	At3g54470
ORF Names:	T14E10_40

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine ribonucleotide biosynthetic process Ref.1 Inferred from genetic interaction. Source: TAIR response to cadmium ion Inferred from expression pattern. Source: TAIR
Molecular function	orotate phosphoribosyltransferase activity Ref.1 Inferred from genetic interaction. Source: TAIR orotidine-5'-phosphate decarboxylase activity Ref.1 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Uridine 5'-monophosphate synthase		PRO_0000139653
Sites
	Active site	305	1	By similarity
Experimental info
	Sequence conflict	186	1	V → E Ref.1
	Sequence conflict	186	1	V → E Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q42586-1 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: F79AE7992503D928	FASTA	476	51,850
	10 20 30 40 50 60 MSAMEALILQ LHEIGAVKFG NFKLKSGIFS PVYIDLRLIV SYPSLLTQIS QTLISSLPPS 70 80 90 100 110 120 ATFDVVCGVP YTALPIATVV SVSNGIPMLM RRKEIKDYGT SKAIEGIFEK DQTCLIIEDL 130 140 150 160 170 180 VTSGASVLET AAPLRAVGLK VSDAVVLIDR QQGGRENLAE NGIKLHSMIM LTDMVRVLKE 190 200 210 220 230 240 KGKIEVEVEV NLLKFLEENR RVSVPSVEKP KPKPRVLGFK ERSELSKNPT GKKLFDIMLK 250 260 270 280 290 300 KETNLCLAAD VGTAAELLDI ADKVGPEICL LKTHVDILPD FTPDFGSKLR AIADKHKFLI 310 320 330 340 350 360 FEDRKFADIG NTVTMQYEGG IFKILEWADI INAHVISGPG IVDGLKLKGM PRGRGLLLLA 370 380 390 400 410 420 EMSSAGNLAT GDYTAAAVKI ADAHSDFVMG FISVNPASWK CGYVYPSMIH ATPGVQMVKG 430 440 450 460 470 GDALGQQYNT PHSVITERGS DIIIVGRGII KAENPAETAH EYRVQGWNAY LEKCSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Heterospecific cloning of Arabidopsis thaliana cDNAs by direct complementation of pyrimidine auxotrophic mutants of Saccharomyces cerevisiae. I. Cloning and sequence analysis of two cDNAs catalysing the second, fifth and sixth steps of the de novo pyrimidine biosynthesis pathway." Nasr F., Bertauche N., Dufour M.E., Minet M., Lacroute F. Mol. Gen. Genet. 244:23-32(1994) [PubMed: 8041358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Transcriptional analysis of the Arabidopsis thaliana UMP synthase locus." Kafer C.W., Thornburg R.W. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X71842 mRNA. Translation: CAA50686.1. AF276887 Genomic DNA. Translation: AAK69440.1. AL138656 Genomic DNA. Translation: CAB77567.1. AY140098 mRNA. Translation: AAM98239.1. BT006612 mRNA. Translation: AAP31956.1.
IPI	IPI00526642.
PIR	S46440. T47606.
RefSeq	NP_680130.1.
UniGene	At.108
3D structure databases
SMR	Q42586. Positions 4-200, 217-473.
ModBase	Search...
Protein-protein interaction databases
STRING	Q42586.
Proteomic databases
PRIDE	Q42586.
Genome annotation databases
GeneID	824612.
GenomeReviews	Gene locus AT3G54470 in contig BA000014_GR.
KEGG	ath:AT3G54470.
NMPDR	fig|3702.1.peg.16747.
Organism-specific databases
TAIR	At3g54470.
Phylogenomic databases
eggNOG	KOG1377.
HOGENOM	HBG326699.
InParanoid	Q42586.
OMA	LFQTAQN.
PhylomeDB	Q42586.
Enzyme and pathway databases
BioCyc	MetaCyc:AT3G54470-MONOMER.
BRENDA	2.4.2.10. 302. 4.1.1.23. 302.
Gene expression databases
ArrayExpress	Q42586.
Genevestigator	Q42586.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase_1_core. IPR018089. OMPdecase_AS. IPR001754. OMPdecase_core. IPR004467. Or_phspho_trans. IPR000836. PRibTrfase. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. pyrE. 1 hit. TIGR01740. pyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYR5_ARATH

Accession

Primary (citable) accession number: Q42586
Secondary accession number(s): Q9M1I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	June 6, 2002
Last modified:	February 9, 2010
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)