Reviewed, UniProtKB/Swiss-Prot Q42583 (KPRS2_ARATH)

Names and origin

Protein names

Recommended name:
    Ribose-phosphate pyrophosphokinase 2
    EC=2.7.6.1
Alternative name(s):
    Phosphoribosyl pyrophosphate synthetase 2
    PRS II

Gene names

Name:	PRS2
Ordered Locus Names:	At1g32380
ORF Names:	F5D14.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Ribose-phosphate pyrophosphokinase 2		PRO_0000141093
Regions
	Region	300 – 315	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	214	1	Magnesium Potential
	Metal binding	216	1	Magnesium Potential
	Metal binding	225	1	Magnesium Potential
	Metal binding	229	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q42583-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: A0BCCA950296E1BF	FASTA	400	43,333
	10 20 30 40 50 60 MASLALTSPP SVKIPSYLSS SSSSLFSRSS ISFRTTESRS RICVSGYAKC NLPKALNGNA 70 80 90 100 110 120 RVPIINETTI PKFFDSSRLE KSVSRNNTKL KLFSGTANPA LSQEIAWYMG LELGKVSIKR 130 140 150 160 170 180 FADGEIYVQL KESVRGCDVF LVQPTCTPTN ENLMELLIMV DACRRASAKK VTAVIPYFGY 190 200 210 220 230 240 ARADRKTQGR ESIAAKLVAN LITEAGADRV LACDLHSGQS MGYFDIPVDH VYCQPVILDY 250 260 270 280 290 300 LASKSISSED LVVVSPDVGG VARARAFAKK LSDAPLAIVD KRRHGHNVAE VMNLIGDVKG 310 320 330 340 350 360 KVAVMVDDII DTAGTIVKGA ALLHEEGARE VYACCTHAVF SPPAIERLSS GLLQEVIVTN 370 380 390 400 TLPVAEKNYF PQLTILSVAN LLGETIWRVH DDSSVSSIFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Cloning and sequencing of cDNAs specifying a novel class of phosphoribosyl diphosphate synthase in Arabidopsis thaliana." Krath B.N., Eriksen T.A., Poulsen T.S., Hove-Jensen B. Biochim. Biophys. Acta 1430:403-408(1999) [PubMed: 10082968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 89-400. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC007767 Genomic DNA. Translation: AAF81335.1. AF439851 mRNA. Translation: AAL27519.1. AY125555 mRNA. Translation: AAM78065.1. X92974 mRNA. Translation: CAA63552.1.
IPI	IPI00519996.
PIR	G86448. S71262.
RefSeq	NP_174516.1.
UniGene	At.423
3D structure databases
HSSP	HSSP built from PDB template 1DKR based on UniProtKB P14193.
ModBase	Search...
Protein-protein interaction databases
STRING	Q42583.
Proteomic databases
PRIDE	Q42583.
ProMEX	Q42583.
Genome annotation databases
GeneID	840131.
GenomeReviews	Gene locus AT1G32380 in contig CT485782_GR.
KEGG	ath:AT1G32380.
NMPDR	fig|3702.1.peg.3591.
Organism-specific databases
GeneFarm	4834. 468.
TAIR	At1g32380.
Phylogenomic databases
OMA	TNTIPLK.
Enzyme and pathway databases
BRENDA	2.7.6.1. 302.
Gene expression databases
Genevestigator	Q42583.
GermOnline	AT1G32380. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR000842. PRib-PP_synthetase_CS. IPR000836. PRibTrfase. IPR005946. PRPP_kinase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. ribP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHETASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS2_ARATH

Accession

Primary (citable) accession number: Q42583
Secondary accession number(s): Q9LQM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)