Q42581 (KPRS1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase 1, chloroplastic
EC=2.7.6.1

Alternative name(s):
PRS I
Phosphoribosyl pyrophosphate synthase 1

Gene names

Name:	PRS1
Ordered Locus Names:	At2g35390
ORF Names:	T32F12.23

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Subcellular location	Plastid › chloroplast Potential.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Sequence caution	The sequence AAC36182.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA58717.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q42581-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q42581-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Note: May be due to an intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 403	354	Ribose-phosphate pyrophosphokinase 1, chloroplastic		PRO_0000141092
Regions
	Region	303 – 318	16	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	217	1	Magnesium Potential
	Metal binding	219	1	Magnesium Potential
	Metal binding	228	1	Magnesium Potential
	Metal binding	232	1	Magnesium Potential
Natural variations
	Alternative sequence	1 – 51	51	Missing in isoform 2.		VSP_013267
Experimental info
	Sequence conflict	178	1	I → T in BAD44564. Ref.5
	Sequence conflict	194	1	E → K in AAO00875. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: B6BFA443773A8D90	FASTA	403	43,695
	10 20 30 40 50 60 MASLGLSFPP AAKTPTYLAS SSSTFFSNSS LSVRTSQFRS RNSVFACVKC DMPESLNVGN 70 80 90 100 110 120 GNPSIPIINE RTLPKFLESA RMEKSVNRTN TRLKLFSGTA NPALAQEIAW YMGLDLGKVN 130 140 150 160 170 180 IKRFADGEIY VQLQESVRGC DVYLVQPTCT PTNENLMELL IMVDACRRAS AKKVTAVIPY 190 200 210 220 230 240 FGYARADRKT QGRESIAAKL VANLITEAGA DRVLACDLHS GQSMGYFDIP VDHVYCQPVI 250 260 270 280 290 300 LDYLASKSIP SEDLVVVSPD VGGVARARAF AKKLSDAPLA IVDKRRSGHN VAEVMNLIGD 310 320 330 340 350 360 VRGKVAIMVD DMIDTAGTIV KGAALLHQEG AREVYACCTH AVFSPPAIER LSGGLLQEVI 370 380 390 400 VTNTLPVAEK NYFPQLTILS VANLLGETIW RVHDDSSVSS IFL
	Isoform 2 [UniParc]. Checksum: 2DE7130C4616A8E3	FASTA	352	38,272
	10 20 30 40 50 60 MPESLNVGNG NPSIPIINER TLPKFLESAR MEKSVNRTNT RLKLFSGTAN PALAQEIAWY 70 80 90 100 110 120 MGLDLGKVNI KRFADGEIYV QLQESVRGCD VYLVQPTCTP TNENLMELLI MVDACRRASA 130 140 150 160 170 180 KKVTAVIPYF GYARADRKTQ GRESIAAKLV ANLITEAGAD RVLACDLHSG QSMGYFDIPV 190 200 210 220 230 240 DHVYCQPVIL DYLASKSIPS EDLVVVSPDV GGVARARAFA KKLSDAPLAI VDKRRSGHNV 250 260 270 280 290 300 AEVMNLIGDV RGKVAIMVDD MIDTAGTIVK GAALLHQEGA REVYACCTHA VFSPPAIERL 310 320 330 340 350 SGGLLQEVIV TNTLPVAEKN YFPQLTILSV ANLLGETIWR VHDDSSVSSI FL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[4]	"Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2 reveals numerous transcript variants." Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L., Ayele M., Haas B.J., Wu H.C., Town C.D. Plant Physiol. 139:1323-1337(2005) [PubMed: 16244158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.
[6]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.
[7]	"Cloning and sequencing of cDNAs specifying a novel class of phosphoribosyl diphosphate synthase in Arabidopsis thaliana." Krath B.N., Eriksen T.A., Poulsen T.S., Hove-Jensen B. Biochim. Biophys. Acta 1430:403-408(1999) [PubMed: 10082968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 41-403 (ISOFORM 1). Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC005314 Genomic DNA. Translation: AAC36182.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC09103.1. CP002685 Genomic DNA. Translation: AEC09104.1. BT002515 mRNA. Translation: AAO00875.1. AY261787 mRNA. Translation: AAP21681.1. AK175326 mRNA. Translation: BAD43089.1. AK175885 mRNA. Translation: BAD43648.1. AK175907 mRNA. Translation: BAD43670.1. AK176491 mRNA. Translation: BAD44254.1. AK176555 mRNA. Translation: BAD44318.1. AK176801 mRNA. Translation: BAD44564.1. AY773861 mRNA. Translation: AAV63890.1. X83764 mRNA. Translation: CAA58717.1. Different initiation.
IPI	IPI00519537. IPI00521887.
PIR	S51270.
RefSeq	NP_181082.1. NM_129091.3. NP_850244.1. NM_179913.2.
UniGene	At.424.
3D structure databases
ProteinModelPortal	Q42581.
SMR	Q42581. Positions 87-402.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42581. 1 interaction.
STRING	Q42581.
Proteomic databases
PRIDE	Q42581.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G35390.2; AT2G35390.2; AT2G35390.
GeneID	818106.
GenomeReviews	Gene locus AT2G35390 in contig CT485783_GR.
KEGG	ath:AT2G35390.
Organism-specific databases
GeneFarm	4833. 468.
TAIR	At2g35390.
Phylogenomic databases
GeneTree	EPGT00070000029078.
HOGENOM	HBG519284.
InParanoid	Q42581.
OMA	NTVPFEE.
PhylomeDB	Q42581.
ProtClustDB	PLN02369.
Enzyme and pathway databases
BioCyc	ARA:AT2G35390-MONOMER.
Gene expression databases
Genevestigator	Q42581.
Family and domain databases
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS1_ARATH

Accession

Primary (citable) accession number: Q42581
Secondary accession number(s): Q67XL7 , Q67YH7, Q84LR8, Q8GUH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 21, 2003
Last modified:	November 16, 2011
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program