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Q42580

- PER21_ARATH

UniProt

Q42580 - PER21_ARATH

Protein

Peroxidase 21

Gene

PER21

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
    Might function as heat shock-like defense protein. May be implicated in the systemic acquired resistance response.

    Catalytic activityi

    2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

    Cofactori

    Binds 1 heme B (iron-protoporphyrin IX) group per subunit.PROSITE-ProRule annotation
    Binds 2 calcium ions per subunit.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei66 – 661Transition state stabilizerPROSITE-ProRule annotation
    Active sitei70 – 701Proton acceptor
    Metal bindingi71 – 711Calcium 1PROSITE-ProRule annotation
    Metal bindingi74 – 741Calcium 1; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi78 – 781Calcium 1PROSITE-ProRule annotation
    Metal bindingi80 – 801Calcium 1PROSITE-ProRule annotation
    Binding sitei167 – 1671Substrate; via carbonyl oxygenPROSITE-ProRule annotation
    Metal bindingi197 – 1971Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi198 – 1981Calcium 2PROSITE-ProRule annotation
    Metal bindingi247 – 2471Calcium 2PROSITE-ProRule annotation
    Metal bindingi250 – 2501Calcium 2PROSITE-ProRule annotation
    Metal bindingi255 – 2551Calcium 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW
    3. peroxidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. defense response to fungus Source: TAIR
    2. hydrogen peroxide catabolic process Source: UniProtKB-KW
    3. rhythmic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Biological processi

    Biological rhythms, Hydrogen peroxide

    Keywords - Ligandi

    Calcium, Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G37130-MONOMER.
    ARA:GQT-999-MONOMER.

    Protein family/group databases

    PeroxiBasei240. AtPrx21.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxidase 21 (EC:1.11.1.7)
    Short name:
    Atperox P21
    Alternative name(s):
    ATP2a/ATP2b
    PRXR5
    Gene namesi
    Name:PER21
    Synonyms:P21
    Ordered Locus Names:At2g37130
    ORF Names:T2N18.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G37130.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 327299Peroxidase 21PRO_0000023687Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi39 ↔ 118PROSITE-ProRule annotation
    Disulfide bondi72 ↔ 77PROSITE-ProRule annotation
    Disulfide bondi124 ↔ 323PROSITE-ProRule annotation
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi204 ↔ 231PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiQ42580.
    PRIDEiQ42580.

    Expressioni

    Tissue specificityi

    Preferentially expressed in roots and leaves, slightly in stems.

    Developmental stagei

    Up-regulated during leaf development.1 Publication

    Inductioni

    Late induced after mechanical wounding. Enhanced expression following incompatible bacterial pathogen attack. Expressed under a diurnal rhythm (circadian clock control).4 Publications

    Gene expression databases

    ArrayExpressiQ42580.
    GenevestigatoriQ42580.

    Interactioni

    Protein-protein interaction databases

    IntActiQ42580. 1 interaction.
    STRINGi3702.AT2G37130.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42580.
    SMRiQ42580. Positions 30-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG297551.
    HOGENOMiHOG000237556.
    InParanoidiQ42580.
    KOiK00430.
    OMAiKDCRYVN.
    PhylomeDBiQ42580.

    Family and domain databases

    InterProiIPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR000823. Peroxidase_pln.
    [Graphical view]
    PfamiPF00141. peroxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00458. PEROXIDASE.
    PR00461. PLPEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 1 hit.
    PROSITEiPS50873. PEROXIDASE_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q42580-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MANAKPFCLL GFFCLLLQLF SIFHIGNGEL EMNYYKESCP KAEEIIRQQV    50
    ETLYYKHGNT AVSWLRNLFH DCVVKSCDAS LLLETARGVE SEQKSKRSFG 100
    MRNFKYVKII KDALEKECPS TVSCADIVAL SARDGIVMLK GPKIEMIKTG 150
    RRDSRGSYLG DVETLIPNHN DSLSSVISTF NSIGIDVEAT VALLGAHSVG 200
    RVHCVNLVHR LYPTIDPTLD PSYALYLKKR CPSPTPDPNA VLYSRNDRET 250
    PMVVDNMYYK NIMAHKGLLV IDDELATDPR TAPFVAKMAA DNNYFHEQFS 300
    RGVRLLSETN PLTGDQGEIR KDCRYVN 327
    Length:327
    Mass (Da):36,741
    Last modified:November 1, 1996 - v1
    Checksum:iD64870D7B8641007
    GO

    Sequence cautioni

    The sequence AAL24415.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti177 – 1771I → L in CAA66961. 1 PublicationCurated
    Sequence conflicti279 – 2791P → S in CAA66961. 1 PublicationCurated
    Sequence conflicti293 – 2931N → G in CAA66961. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98317 mRNA. Translation: CAA66961.1.
    X98190 mRNA. Translation: CAA66863.1.
    AC006260 Genomic DNA. Translation: AAD18146.1.
    CP002685 Genomic DNA. Translation: AEC09354.1.
    AY087458 mRNA. Translation: AAM65003.1.
    AY059933 mRNA. Translation: AAL24415.1. Different initiation.
    AY081588 mRNA. Translation: AAM10150.1.
    PIRiH84788.
    RefSeqiNP_181250.1. NM_129269.3. [Q42580-1]
    UniGeneiAt.21472.
    At.47592.

    Genome annotation databases

    EnsemblPlantsiAT2G37130.1; AT2G37130.1; AT2G37130. [Q42580-1]
    GeneIDi818289.
    KEGGiath:AT2G37130.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X98317 mRNA. Translation: CAA66961.1 .
    X98190 mRNA. Translation: CAA66863.1 .
    AC006260 Genomic DNA. Translation: AAD18146.1 .
    CP002685 Genomic DNA. Translation: AEC09354.1 .
    AY087458 mRNA. Translation: AAM65003.1 .
    AY059933 mRNA. Translation: AAL24415.1 . Different initiation.
    AY081588 mRNA. Translation: AAM10150.1 .
    PIRi H84788.
    RefSeqi NP_181250.1. NM_129269.3. [Q42580-1 ]
    UniGenei At.21472.
    At.47592.

    3D structure databases

    ProteinModelPortali Q42580.
    SMRi Q42580. Positions 30-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q42580. 1 interaction.
    STRINGi 3702.AT2G37130.1-P.

    Protein family/group databases

    PeroxiBasei 240. AtPrx21.

    Proteomic databases

    PaxDbi Q42580.
    PRIDEi Q42580.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G37130.1 ; AT2G37130.1 ; AT2G37130 . [Q42580-1 ]
    GeneIDi 818289.
    KEGGi ath:AT2G37130.

    Organism-specific databases

    GeneFarmi 1845. 61.
    TAIRi AT2G37130.

    Phylogenomic databases

    eggNOGi NOG297551.
    HOGENOMi HOG000237556.
    InParanoidi Q42580.
    KOi K00430.
    OMAi KDCRYVN.
    PhylomeDBi Q42580.

    Enzyme and pathway databases

    BioCyci ARA:AT2G37130-MONOMER.
    ARA:GQT-999-MONOMER.

    Gene expression databases

    ArrayExpressi Q42580.
    Genevestigatori Q42580.

    Family and domain databases

    InterProi IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR000823. Peroxidase_pln.
    [Graphical view ]
    Pfami PF00141. peroxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00458. PEROXIDASE.
    PR00461. PLPEROXIDASE.
    SUPFAMi SSF48113. SSF48113. 1 hit.
    PROSITEi PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases."
      Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P.
      Plant Gene Register PGR96-066
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and RT-PCR expression analysis of two peroxidases from Arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases."
      Kjaersgaard I.V.H., Jespersen H.M., Rasmussen S.K., Welinder K.G.
      Plant Mol. Biol. 33:699-708(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-327.
      Strain: cv. Columbia.
    7. "Computational analyses and annotations of the Arabidopsis peroxidase gene family."
      Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
      FEBS Lett. 433:98-102(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: cv. Columbia.
    8. "The transcriptome of Arabidopsis thaliana during systemic acquired resistance."
      Maleck K., Levine A., Eulgem T., Morgan A., Schmid J., Lawton K.A., Dangl J.L., Dietrich R.A.
      Nat. Genet. 26:403-410(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: cv. Wassilewskija.
    9. "Orchestrated transcription of key pathways in Arabidopsis by the circadian clock."
      Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A.
      Science 290:2110-2113(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: cv. Columbia.
    10. "Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis."
      Schaffer R., Landgraf J., Accerbi M., Simon V., Larson M., Wisman E.
      Plant Cell 13:113-123(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: cv. Columbia.
    11. "Toward elucidating the global gene expression patterns of developing Arabidopsis: parallel analysis of 8300 genes by a high-density oligonucleotide probe array."
      Zhu T., Budworth P., Han B., Brown D., Chang H.-S., Zou G., Wang X.
      Plant Physiol. Biochem. 39:221-242(2001)
      Cited for: DEVELOPMENTAL STAGE.
      Strain: cv. Columbia.
    12. "Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis."
      Cheong Y.H., Chang H.-S., Gupta R., Wang X., Zhu T., Luan S.
      Plant Physiol. 129:661-677(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
      Strain: cv. Columbia.
    13. "Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
      Tognolli M., Penel C., Greppin H., Simon P.
      Gene 288:129-138(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPER21_ARATH
    AccessioniPrimary (citable) accession number: Q42580
    Secondary accession number(s): Q43733, Q93YM9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2002
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 128 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    There are 73 peroxidase genes in A.thaliana.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3