Peroxidase 21 precursor - Arabidopsis thaliana (Mouse-ear cress)

Contribute

Send feedback

Reviewed, UniProtKB/Swiss-Prot Q42580 (PER21_ARATH)

Last modified November 25, 2008. Version 80. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Peroxidase 21
      Short name=Atperox P21
    EC=1.11.1.7
Alternative name(s):
    PRXR5
    ATP2a/ATP2b

Gene names

Name:	PER21
Synonyms:	P21
Ordered Locus Names:	At2g37130
ORF Names:	T2N18.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Hide | Top

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Hide | Top

General annotation (Comments)

Function	Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. Might function as heat shock-like defense protein. May be implicated in the systemic acquired resistance response.
Catalytic activity	Donor + H(2)O(2) = oxidized donor + 2 H(2)O.
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity. Binds 2 calcium ions per subunit By similarity.
Tissue specificity	Preferentially expressed in roots and leaves, slightly in stems.
Developmental stage	Up-regulated during leaf development.
Induction	Late induced after mechanical wounding. Enhanced expression following incompatible bacterial pathogen attack. Expressed under a diurnal rhythm (circadian clock control).
Miscellaneous	There are 73 peroxidase genes in A.thaliana.
Sequence similarities	Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Hide | Top

Ontologies

Keywords
Biological process	Biological rhythms Hydrogen peroxide
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peroxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Hide | Top

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select] Notes: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42580-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 28

Potential

Chain

29 – 327

299

Peroxidase 21

PRO_0000023687

Sites

Active site

Proton acceptor

Metal binding

Calcium 1 By similarity

Metal binding

Calcium 1; via carbonyl oxygen By similarity

Metal binding

Calcium 1 By similarity

Metal binding

Calcium 1 By similarity

Metal binding

197

Iron (heme axial ligand) By similarity

Metal binding

198

Calcium 2 By similarity

Metal binding

247

Calcium 2 By similarity

Metal binding

250

Calcium 2 By similarity

Metal binding

255

Calcium 2 By similarity

Binding site

167

Substrate; via carbonyl oxygen By similarity

Site

Transition state stabilizer By similarity

Amino acid modifications

Glycosylation

170

N-linked (GlcNAc...) Potential

Disulfide bond

39 ↔ 118

By similarity

Disulfide bond

72 ↔ 77

By similarity

Disulfide bond

124 ↔ 323

By similarity

Disulfide bond

204 ↔ 231

By similarity

Experimental info

Sequence conflict

177

I → L in CAA66961. Ref.1

Sequence conflict

279

P → S in CAA66961. Ref.1

Sequence conflict

293

N → G in CAA66961. Ref.1

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D64870D7B8641007
Show »

FASTA

327

36,741

        10         20         30         40         50         60 
MANAKPFCLL GFFCLLLQLF SIFHIGNGEL EMNYYKESCP KAEEIIRQQV ETLYYKHGNT 

        70         80         90        100        110        120 
AVSWLRNLFH DCVVKSCDAS LLLETARGVE SEQKSKRSFG MRNFKYVKII KDALEKECPS 

       130        140        150        160        170        180 
TVSCADIVAL SARDGIVMLK GPKIEMIKTG RRDSRGSYLG DVETLIPNHN DSLSSVISTF 

       190        200        210        220        230        240 
NSIGIDVEAT VALLGAHSVG RVHCVNLVHR LYPTIDPTLD PSYALYLKKR CPSPTPDPNA 

       250        260        270        280        290        300 
VLYSRNDRET PMVVDNMYYK NIMAHKGLLV IDDELATDPR TAPFVAKMAA DNNYFHEQFS 

       310        320 
RGVRLLSETN PLTGDQGEIR KDCRYVN

« Hide

Hide | Top

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases." Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P. (er) Plant Gene Register PGR96-066 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and RT-PCR expression analysis of two peroxidases from Arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases." Kjaersgaard I.V.H., Jespersen H.M., Rasmussen S.K., Welinder K.G. Plant Mol. Biol. 33:699-708(1997) [PubMed: 9132061] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-327. Strain: cv. Columbia.
[6]	"Computational analyses and annotations of the Arabidopsis peroxidase gene family." Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G. FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract] Cited for: CHARACTERIZATION. Strain: cv. Columbia.
[7]	"The transcriptome of Arabidopsis thaliana during systemic acquired resistance." Maleck K., Levine A., Eulgem T., Morgan A., Schmid J., Lawton K.A., Dangl J.L., Dietrich R.A. Nat. Genet. 26:403-410(2000) [PubMed: 11101835] [Abstract] Cited for: INDUCTION. Strain: cv. Wassilewskija.
[8]	"Orchestrated transcription of key pathways in Arabidopsis by the circadian clock." Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A. Science 290:2110-2113(2000) [PubMed: 11118138] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[9]	"Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis." Schaffer R., Landgraf J., Accerbi M., Simon V., Larson M., Wisman E. Plant Cell 13:113-123(2001) [PubMed: 11158533] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[10]	"Toward elucidating the global gene expression patterns of developing Arabidopsis: parallel analysis of 8300 genes by a high-density oligonucleotide probe array." Zhu T., Budworth P., Han B., Brown D., Chang H.-S., Zou G., Wang X. Plant Physiol. Biochem. 39:221-242(2001) Cited for: DEVELOPMENTAL STAGE. Strain: cv. Columbia.
[11]	"Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis." Cheong Y.H., Chang H.-S., Gupta R., Wang X., Zhu T., Luan S. Plant Physiol. 129:661-677(2002) [PubMed: 12068110] [Abstract] Cited for: INDUCTION. Strain: cv. Columbia.
[12]	"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana." Tognolli M., Penel C., Greppin H., Simon P. Gene 288:129-138(2002) [PubMed: 12034502] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE. Strain: cv. Columbia.

Hide | Top

Cross-references

Sequence databases
	X98317 mRNA. Translation: CAA66961.1. X98190 mRNA. Translation: CAA66863.1. AC006260 Genomic DNA. Translation: AAD18146.1. AY087458 mRNA. Translation: AAM65003.1. AY059933 mRNA. Translation: AAL24415.1. Different initiation. AY081588 mRNA. Translation: AAM10150.1.
PIR	H84788.
RefSeq	NP_181250.1.
UniGene	At.47592
3D structure databases
HSSP	HSSP built from PDB template 1SCH based on UniProtKB P22195.
ModBase	Search...
Protein family/group databases
PeroxiBase	240. AtPrx21.
Genome annotation databases
GeneID	818289.
GenomeReviews	Gene locus AT2G37130 in contig CT485783_GR.
NMPDR	fig\|3702.1.peg.10814.
Organism-specific databases
GeneFarm	1845. 61.
TAIR	At2g37130.
Gene expression databases
ArrayExpress	Q42580.
GermOnline	AT2G37130. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002016. Haem_peroxidase_pln/fun/bac. IPR000823. Peroxidase_pln. [Graphical view]
Pfam	PF00141. peroxidase. 1 hit. [Graphical view]
PRINTS	PR00458. PEROXIDASE. PR00461. PLPEROXIDASE.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. False negative. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

PER21_ARATH

Accession

Primary (citable) accession number: Q42580
Secondary accession number(s): Q43733, Q93YM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2002
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Hide | Top

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Alternative products

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents