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Q42580

- PER21_ARATH

UniProt

Q42580 - PER21_ARATH

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Protein
Peroxidase 21
Gene
PER21, P21, At2g37130, T2N18.11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Might function as heat shock-like defense protein. May be implicated in the systemic acquired resistance response.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.
Binds 2 calcium ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei66 – 661Transition state stabilizer By similarity
Active sitei70 – 701Proton acceptor
Metal bindingi71 – 711Calcium 1 By similarity
Metal bindingi74 – 741Calcium 1; via carbonyl oxygen By similarity
Metal bindingi78 – 781Calcium 1 By similarity
Metal bindingi80 – 801Calcium 1 By similarity
Binding sitei167 – 1671Substrate; via carbonyl oxygen By similarity
Metal bindingi197 – 1971Iron (heme axial ligand) By similarity
Metal bindingi198 – 1981Calcium 2 By similarity
Metal bindingi247 – 2471Calcium 2 By similarity
Metal bindingi250 – 2501Calcium 2 By similarity
Metal bindingi255 – 2551Calcium 2 By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
  3. peroxidase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. defense response to fungus Source: TAIR
  2. hydrogen peroxide catabolic process Source: UniProtKB-KW
  3. rhythmic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Biological rhythms, Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G37130-MONOMER.
ARA:GQT-999-MONOMER.

Protein family/group databases

PeroxiBasei240. AtPrx21.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 21 (EC:1.11.1.7)
Short name:
Atperox P21
Alternative name(s):
ATP2a/ATP2b
PRXR5
Gene namesi
Name:PER21
Synonyms:P21
Ordered Locus Names:At2g37130
ORF Names:T2N18.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G37130.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed prediction
Add
BLAST
Chaini29 – 327299Peroxidase 21
PRO_0000023687Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 118 By similarity
Disulfide bondi72 ↔ 77 By similarity
Disulfide bondi124 ↔ 323 By similarity
Glycosylationi170 – 1701N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi204 ↔ 231 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ42580.
PRIDEiQ42580.

Expressioni

Tissue specificityi

Preferentially expressed in roots and leaves, slightly in stems.

Developmental stagei

Up-regulated during leaf development.1 Publication

Inductioni

Late induced after mechanical wounding. Enhanced expression following incompatible bacterial pathogen attack. Expressed under a diurnal rhythm (circadian clock control).4 Publications

Gene expression databases

ArrayExpressiQ42580.
GenevestigatoriQ42580.

Interactioni

Protein-protein interaction databases

IntActiQ42580. 1 interaction.
STRINGi3702.AT2G37130.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ42580.
SMRiQ42580. Positions 30-327.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG297551.
HOGENOMiHOG000237556.
InParanoidiQ42580.
KOiK00430.
OMAiKDCRYVN.
PhylomeDBiQ42580.

Family and domain databases

InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42580-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MANAKPFCLL GFFCLLLQLF SIFHIGNGEL EMNYYKESCP KAEEIIRQQV    50
ETLYYKHGNT AVSWLRNLFH DCVVKSCDAS LLLETARGVE SEQKSKRSFG 100
MRNFKYVKII KDALEKECPS TVSCADIVAL SARDGIVMLK GPKIEMIKTG 150
RRDSRGSYLG DVETLIPNHN DSLSSVISTF NSIGIDVEAT VALLGAHSVG 200
RVHCVNLVHR LYPTIDPTLD PSYALYLKKR CPSPTPDPNA VLYSRNDRET 250
PMVVDNMYYK NIMAHKGLLV IDDELATDPR TAPFVAKMAA DNNYFHEQFS 300
RGVRLLSETN PLTGDQGEIR KDCRYVN 327
Length:327
Mass (Da):36,741
Last modified:November 1, 1996 - v1
Checksum:iD64870D7B8641007
GO

Sequence cautioni

The sequence AAL24415.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti177 – 1771I → L in CAA66961. 1 Publication
Sequence conflicti279 – 2791P → S in CAA66961. 1 Publication
Sequence conflicti293 – 2931N → G in CAA66961. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98317 mRNA. Translation: CAA66961.1.
X98190 mRNA. Translation: CAA66863.1.
AC006260 Genomic DNA. Translation: AAD18146.1.
CP002685 Genomic DNA. Translation: AEC09354.1.
AY087458 mRNA. Translation: AAM65003.1.
AY059933 mRNA. Translation: AAL24415.1. Different initiation.
AY081588 mRNA. Translation: AAM10150.1.
PIRiH84788.
RefSeqiNP_181250.1. NM_129269.3. [Q42580-1]
UniGeneiAt.21472.
At.47592.

Genome annotation databases

EnsemblPlantsiAT2G37130.1; AT2G37130.1; AT2G37130. [Q42580-1]
GeneIDi818289.
KEGGiath:AT2G37130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X98317 mRNA. Translation: CAA66961.1 .
X98190 mRNA. Translation: CAA66863.1 .
AC006260 Genomic DNA. Translation: AAD18146.1 .
CP002685 Genomic DNA. Translation: AEC09354.1 .
AY087458 mRNA. Translation: AAM65003.1 .
AY059933 mRNA. Translation: AAL24415.1 . Different initiation.
AY081588 mRNA. Translation: AAM10150.1 .
PIRi H84788.
RefSeqi NP_181250.1. NM_129269.3. [Q42580-1 ]
UniGenei At.21472.
At.47592.

3D structure databases

ProteinModelPortali Q42580.
SMRi Q42580. Positions 30-327.
ModBasei Search...

Protein-protein interaction databases

IntActi Q42580. 1 interaction.
STRINGi 3702.AT2G37130.1-P.

Protein family/group databases

PeroxiBasei 240. AtPrx21.

Proteomic databases

PaxDbi Q42580.
PRIDEi Q42580.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G37130.1 ; AT2G37130.1 ; AT2G37130 . [Q42580-1 ]
GeneIDi 818289.
KEGGi ath:AT2G37130.

Organism-specific databases

GeneFarmi 1845. 61.
TAIRi AT2G37130.

Phylogenomic databases

eggNOGi NOG297551.
HOGENOMi HOG000237556.
InParanoidi Q42580.
KOi K00430.
OMAi KDCRYVN.
PhylomeDBi Q42580.

Enzyme and pathway databases

BioCyci ARA:AT2G37130-MONOMER.
ARA:GQT-999-MONOMER.

Gene expression databases

ArrayExpressi Q42580.
Genevestigatori Q42580.

Family and domain databases

InterProi IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
[Graphical view ]
Pfami PF00141. peroxidase. 1 hit.
[Graphical view ]
PRINTSi PR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMi SSF48113. SSF48113. 1 hit.
PROSITEi PS50873. PEROXIDASE_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases."
    Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P.
    Plant Gene Register PGR96-066
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and RT-PCR expression analysis of two peroxidases from Arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases."
    Kjaersgaard I.V.H., Jespersen H.M., Rasmussen S.K., Welinder K.G.
    Plant Mol. Biol. 33:699-708(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-327.
    Strain: cv. Columbia.
  7. "Computational analyses and annotations of the Arabidopsis peroxidase gene family."
    Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
    FEBS Lett. 433:98-102(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: cv. Columbia.
  8. "The transcriptome of Arabidopsis thaliana during systemic acquired resistance."
    Maleck K., Levine A., Eulgem T., Morgan A., Schmid J., Lawton K.A., Dangl J.L., Dietrich R.A.
    Nat. Genet. 26:403-410(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Wassilewskija.
  9. "Orchestrated transcription of key pathways in Arabidopsis by the circadian clock."
    Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A.
    Science 290:2110-2113(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  10. "Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis."
    Schaffer R., Landgraf J., Accerbi M., Simon V., Larson M., Wisman E.
    Plant Cell 13:113-123(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  11. "Toward elucidating the global gene expression patterns of developing Arabidopsis: parallel analysis of 8300 genes by a high-density oligonucleotide probe array."
    Zhu T., Budworth P., Han B., Brown D., Chang H.-S., Zou G., Wang X.
    Plant Physiol. Biochem. 39:221-242(2001)
    Cited for: DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  12. "Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis."
    Cheong Y.H., Chang H.-S., Gupta R., Wang X., Zhu T., Luan S.
    Plant Physiol. 129:661-677(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: cv. Columbia.
  13. "Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
    Tognolli M., Penel C., Greppin H., Simon P.
    Gene 288:129-138(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPER21_ARATH
AccessioniPrimary (citable) accession number: Q42580
Secondary accession number(s): Q43733, Q93YM9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi