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Reviewed, UniProtKB/Swiss-Prot Q42580 (PER21_ARATH)

Last modified November 25, 2008. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Peroxidase 21
      Short name=Atperox P21
    EC=1.11.1.7
Alternative name(s):
    PRXR5
    ATP2a/ATP2b
Gene names
Name: PER21
Synonyms: P21
Ordered Locus Names: At2g37130
ORF Names: T2N18.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Removal of H(2)O(2), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

Might function as heat shock-like defense protein. May be implicated in the systemic acquired resistance response.

Catalytic activity

Donor + H(2)O(2) = oxidized donor + 2 H(2)O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Tissue specificity

Preferentially expressed in roots and leaves, slightly in stems.

Developmental stage

Up-regulated during leaf development.

Induction

Late induced after mechanical wounding. Enhanced expression following incompatible bacterial pathogen attack. Expressed under a diurnal rhythm (circadian clock control).

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Sequence similarities

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Notes: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42580-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 327299Peroxidase 21
PRO_0000023687

Sites

Active site701Proton acceptor
Metal binding711Calcium 1 By similarity
Metal binding741Calcium 1; via carbonyl oxygen By similarity
Metal binding781Calcium 1 By similarity
Metal binding801Calcium 1 By similarity
Metal binding1971Iron (heme axial ligand) By similarity
Metal binding1981Calcium 2 By similarity
Metal binding2471Calcium 2 By similarity
Metal binding2501Calcium 2 By similarity
Metal binding2551Calcium 2 By similarity
Binding site1671Substrate; via carbonyl oxygen By similarity
Site661Transition state stabilizer By similarity

Amino acid modifications

Glycosylation1701N-linked (GlcNAc...) Potential
Disulfide bond39 ↔ 118 By similarity
Disulfide bond72 ↔ 77 By similarity
Disulfide bond124 ↔ 323 By similarity
Disulfide bond204 ↔ 231 By similarity

Experimental info

Sequence conflict1771I → L in CAA66961. Ref.1
Sequence conflict2791P → S in CAA66961. Ref.1
Sequence conflict2931N → G in CAA66961. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D64870D7B8641007

FASTA32736,741
        10         20         30         40         50         60 
MANAKPFCLL GFFCLLLQLF SIFHIGNGEL EMNYYKESCP KAEEIIRQQV ETLYYKHGNT 

        70         80         90        100        110        120 
AVSWLRNLFH DCVVKSCDAS LLLETARGVE SEQKSKRSFG MRNFKYVKII KDALEKECPS 

       130        140        150        160        170        180 
TVSCADIVAL SARDGIVMLK GPKIEMIKTG RRDSRGSYLG DVETLIPNHN DSLSSVISTF 

       190        200        210        220        230        240 
NSIGIDVEAT VALLGAHSVG RVHCVNLVHR LYPTIDPTLD PSYALYLKKR CPSPTPDPNA 

       250        260        270        280        290        300 
VLYSRNDRET PMVVDNMYYK NIMAHKGLLV IDDELATDPR TAPFVAKMAA DNNYFHEQFS 

       310        320 
RGVRLLSETN PLTGDQGEIR KDCRYVN 

« Hide

References

« Hide 'large scale' references
[1]"Eleven cDNA clones from Arabidopsis thaliana encoding isoperoxidases."
Capelli N., Tognolli M., Flach J., Overney S., Penel C., Greppin H., Simon P.
(er) Plant Gene Register PGR96-066
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and RT-PCR expression analysis of two peroxidases from Arabidopsis thaliana belonging to a novel evolutionary branch of plant peroxidases."
Kjaersgaard I.V.H., Jespersen H.M., Rasmussen S.K., Welinder K.G.
Plant Mol. Biol. 33:699-708(1997) [PubMed: 9132061] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 33-327.
Strain: cv. Columbia.
[6]"Computational analyses and annotations of the Arabidopsis peroxidase gene family."
Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract]
Cited for: CHARACTERIZATION.
Strain: cv. Columbia.
[7]"The transcriptome of Arabidopsis thaliana during systemic acquired resistance."
Maleck K., Levine A., Eulgem T., Morgan A., Schmid J., Lawton K.A., Dangl J.L., Dietrich R.A.
Nat. Genet. 26:403-410(2000) [PubMed: 11101835] [Abstract]
Cited for: INDUCTION.
Strain: cv. Wassilewskija.
[8]"Orchestrated transcription of key pathways in Arabidopsis by the circadian clock."
Harmer S.L., Hogenesch J.B., Straume M., Chang H.-S., Han B., Zhu T., Wang X., Kreps J.A., Kay S.A.
Science 290:2110-2113(2000) [PubMed: 11118138] [Abstract]
Cited for: INDUCTION.
Strain: cv. Columbia.
[9]"Microarray analysis of diurnal and circadian-regulated genes in Arabidopsis."
Schaffer R., Landgraf J., Accerbi M., Simon V., Larson M., Wisman E.
Plant Cell 13:113-123(2001) [PubMed: 11158533] [Abstract]
Cited for: INDUCTION.
Strain: cv. Columbia.
[10]"Toward elucidating the global gene expression patterns of developing Arabidopsis: parallel analysis of 8300 genes by a high-density oligonucleotide probe array."
Zhu T., Budworth P., Han B., Brown D., Chang H.-S., Zou G., Wang X.
Plant Physiol. Biochem. 39:221-242(2001)
Cited for: DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
[11]"Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis."
Cheong Y.H., Chang H.-S., Gupta R., Wang X., Zhu T., Luan S.
Plant Physiol. 129:661-677(2002) [PubMed: 12068110] [Abstract]
Cited for: INDUCTION.
Strain: cv. Columbia.
[12]"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
Tognolli M., Penel C., Greppin H., Simon P.
Gene 288:129-138(2002) [PubMed: 12034502] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.

Cross-references

Sequence databases

X98317 mRNA. Translation: CAA66961.1.
X98190 mRNA. Translation: CAA66863.1.
AC006260 Genomic DNA. Translation: AAD18146.1.
AY087458 mRNA. Translation: AAM65003.1.
AY059933 mRNA. Translation: AAL24415.1. Different initiation.
AY081588 mRNA. Translation: AAM10150.1.
PIRH84788.
RefSeqNP_181250.1.
UniGeneAt.47592

3D structure databases

HSSPHSSP built from PDB template 1SCH based on UniProtKB P22195.
ModBaseSearch...

Protein family/group databases

PeroxiBase240. AtPrx21.

Genome annotation databases

GeneID818289.
GenomeReviewsGene locus AT2G37130 in contig CT485783_GR.
NMPDRfig|3702.1.peg.10814.

Organism-specific databases

GeneFarm1845. 61.
TAIRAt2g37130.

Gene expression databases

ArrayExpressQ42580.
GermOnlineAT2G37130. Arabidopsis thaliana.

Family and domain databases

InterProIPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
[Graphical view]
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
PROSITEPS00435. PEROXIDASE_1. False negative.
PS00436. PEROXIDASE_2. False negative.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePER21_ARATH
AccessionPrimary (citable) accession number: Q42580
Secondary accession number(s): Q43733, Q93YM9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 25, 2002
Last sequence update: November 1, 1996
Last modified: November 25, 2008
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents