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Protein

Peroxidase 53

Gene

PER53

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
Closely linked to lignin formation by showing monolignol substrate specificity.

Catalytic activityi

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+Note: Binds 2 calcium ions per subunit.
  • heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei68Transition state stabilizer1
Active sitei72Proton acceptor1
Metal bindingi73Calcium 11
Metal bindingi76Calcium 1; via carbonyl oxygen1
Metal bindingi78Calcium 1; via carbonyl oxygen1
Metal bindingi80Calcium 11
Metal bindingi82Calcium 11
Binding sitei169Substrate; via carbonyl oxygen1
Metal bindingi199Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi200Calcium 21
Metal bindingi251Calcium 21
Metal bindingi254Calcium 21
Metal bindingi259Calcium 21

GO - Molecular functioni

GO - Biological processi

  • defense response to nematode Source: TAIR
  • flower development Source: TAIR
  • hydrogen peroxide catabolic process Source: UniProtKB-KW
  • response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Biological processi

Hydrogen peroxide

Keywords - Ligandi

Calcium, Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G06720-MONOMER.

Protein family/group databases

PeroxiBasei219. AtPrx53.

Names & Taxonomyi

Protein namesi
Recommended name:
Peroxidase 53 (EC:1.11.1.7)
Short name:
Atperox P53
Alternative name(s):
ATPA2
Gene namesi
Name:PER53
Synonyms:P53
Ordered Locus Names:At5g06720
ORF Names:MPH15.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G06720.

Subcellular locationi

  • Secreted PROSITE-ProRule annotation

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • Golgi apparatus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000002371831 – 335Peroxidase 53Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31Pyrrolidone carboxylic acidPROSITE-ProRule annotation1 Publication1
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi41 ↔ 121PROSITE-ProRule annotation
Glycosylationi43N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi74 ↔ 79PROSITE-ProRule annotation
Disulfide bondi127 ↔ 329PROSITE-ProRule annotation
Glycosylationi165N-linked (GlcNAc...)Sequence analysis1
Glycosylationi177N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi206 ↔ 238PROSITE-ProRule annotation
Glycosylationi215N-linked (GlcNAc...)Sequence analysis1
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi241N-linked (GlcNAc...)Sequence analysis1
Glycosylationi297N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ42578.
PRIDEiQ42578.

Expressioni

Tissue specificityi

Mainly expressed in roots.

Gene expression databases

GenevisibleiQ42578. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT5G06720.1.

Structurei

Secondary structure

1335
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni34 – 40Combined sources7
Helixi44 – 56Combined sources13
Helixi62 – 74Combined sources13
Turni75 – 77Combined sources3
Beta strandi78 – 81Combined sources4
Helixi82 – 84Combined sources3
Helixi94 – 96Combined sources3
Turni98 – 103Combined sources6
Helixi107 – 120Combined sources14
Turni122 – 124Combined sources3
Helixi127 – 141Combined sources15
Helixi161 – 167Combined sources7
Helixi175 – 184Combined sources10
Helixi189 – 196Combined sources8
Helixi197 – 200Combined sources4
Beta strandi201 – 205Combined sources5
Helixi206 – 208Combined sources3
Helixi210 – 212Combined sources3
Helixi216 – 218Combined sources3
Beta strandi219 – 221Combined sources3
Helixi228 – 237Combined sources10
Beta strandi247 – 250Combined sources4
Beta strandi252 – 257Combined sources6
Helixi261 – 267Combined sources7
Helixi274 – 281Combined sources8
Helixi287 – 296Combined sources10
Helixi298 – 313Combined sources16
Beta strandi322 – 324Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PA2X-ray1.45A31-335[»]
1QO4X-ray3.00A31-335[»]
ProteinModelPortaliQ42578.
SMRiQ42578.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ42578.

Family & Domainsi

Sequence similaritiesi

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IK8B. Eukaryota.
ENOG410YBAI. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ42578.
KOiK00430.
OMAiMAVTNLP.
OrthoDBiEOG09360FST.
PhylomeDBiQ42578.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVTNLPTCD GLFIISLIVI VSSIFGTSSA QLNATFYSGT CPNASAIVRS
60 70 80 90 100
TIQQALQSDT RIGASLIRLH FHDCFVNGCD ASILLDDTGS IQSEKNAGPN
110 120 130 140 150
VNSARGFNVV DNIKTALENA CPGVVSCSDV LALASEASVS LAGGPSWTVL
160 170 180 190 200
LGRRDSLTAN LAGANSSIPS PIESLSNITF KFSAVGLNTN DLVALSGAHT
210 220 230 240 250
FGRARCGVFN NRLFNFSGTG NPDPTLNSTL LSTLQQLCPQ NGSASTITNL
260 270 280 290 300
DLSTPDAFDN NYFANLQSND GLLQSDQELF STTGSSTIAI VTSFASNQTL
310 320 330
FFQAFAQSMI NMGNISPLTG SNGEIRLDCK KVNGS
Length:335
Mass (Da):34,989
Last modified:November 1, 1996 - v1
Checksum:i57E375B212F1BC6B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21V → L in AAM65211 (Ref. 5) Curated1
Sequence conflicti172I → V in AAM65211 (Ref. 5) Curated1
Sequence conflicti180F → S in AAM65211 (Ref. 5) Curated1
Sequence conflicti270D → N in AAM65211 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99952 mRNA. Translation: CAA68212.1.
AP002032 Genomic DNA. Translation: BAB09806.1.
CP002688 Genomic DNA. Translation: AED91055.1.
AY056186 mRNA. Translation: AAL07035.1.
AY096713 mRNA. Translation: AAM20347.1.
AY087674 mRNA. Translation: AAM65211.1.
RefSeqiNP_196290.1. NM_120755.3.
UniGeneiAt.93.

Genome annotation databases

EnsemblPlantsiAT5G06720.1; AT5G06720.1; AT5G06720.
GeneIDi830561.
GrameneiAT5G06720.1; AT5G06720.1; AT5G06720.
KEGGiath:AT5G06720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99952 mRNA. Translation: CAA68212.1.
AP002032 Genomic DNA. Translation: BAB09806.1.
CP002688 Genomic DNA. Translation: AED91055.1.
AY056186 mRNA. Translation: AAL07035.1.
AY096713 mRNA. Translation: AAM20347.1.
AY087674 mRNA. Translation: AAM65211.1.
RefSeqiNP_196290.1. NM_120755.3.
UniGeneiAt.93.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PA2X-ray1.45A31-335[»]
1QO4X-ray3.00A31-335[»]
ProteinModelPortaliQ42578.
SMRiQ42578.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G06720.1.

Protein family/group databases

PeroxiBasei219. AtPrx53.

Proteomic databases

PaxDbiQ42578.
PRIDEiQ42578.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G06720.1; AT5G06720.1; AT5G06720.
GeneIDi830561.
GrameneiAT5G06720.1; AT5G06720.1; AT5G06720.
KEGGiath:AT5G06720.

Organism-specific databases

TAIRiAT5G06720.

Phylogenomic databases

eggNOGiENOG410IK8B. Eukaryota.
ENOG410YBAI. LUCA.
HOGENOMiHOG000237557.
InParanoidiQ42578.
KOiK00430.
OMAiMAVTNLP.
OrthoDBiEOG09360FST.
PhylomeDBiQ42578.

Enzyme and pathway databases

BioCyciARA:AT5G06720-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ42578.
PROiQ42578.

Gene expression databases

GenevisibleiQ42578. AT.

Family and domain databases

CDDicd00693. secretory_peroxidase. 1 hit.
InterProiIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
IPR033905. Secretory_peroxidase.
[Graphical view]
PfamiPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSiPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPER53_ARATH
AccessioniPrimary (citable) accession number: Q42578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.