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Protein

L-ascorbate peroxidase 3

Gene

APX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Transition state stabilizerPROSITE-ProRule annotation1
Active sitei40Proton acceptorPROSITE-ProRule annotation1
Metal bindingi160Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi161Potassium or calciumBy similarity1
Metal bindingi177Potassium or calciumBy similarity1
Metal bindingi184Potassium or calciumBy similarity1

GO - Molecular functioni

  • cytochrome-c peroxidase activity Source: GO_Central
  • heme binding Source: InterPro
  • L-ascorbate peroxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • hydrogen peroxide catabolic process Source: GO_Central
  • response to cytokinin Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to reactive oxygen species Source: GO_Central

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT4G35000-MONOMER.

Protein family/group databases

PeroxiBasei1891. AtAPx03.

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 3 (EC:1.11.1.11)
Short name:
AtAPx03
Gene namesi
Name:APX3
Synonyms:APX, APXIII
Ordered Locus Names:At4g35000
ORF Names:M4E13.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRilocus:2131586. AT4G35000.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • glyoxysomal membrane Source: UniProtKB-SubCell
  • glyoxysome Source: UniProtKB-KW
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrion Source: TAIR
  • peroxisomal membrane Source: TAIR
  • peroxisome Source: UniProtKB
  • plasmodesma Source: TAIR
  • plastid Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR

Keywords - Cellular componenti

Glyoxysome, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002613232 – 287L-ascorbate peroxidase 3Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ42564.
PRIDEiQ42564.

Expressioni

Gene expression databases

GenevisibleiQ42564. AT.

Interactioni

Subunit structurei

Interacts via its C-terminal region with AKR2A and AKR2B.2 Publications

Protein-protein interaction databases

BioGridi14934. 3 interactors.
STRINGi3702.AT4G35000.1.

Structurei

3D structure databases

ProteinModelPortaliQ42564.
SMRiQ42564.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi281 – 287AKR2A-binding sequence (ABS) required for peroxisome membrane targeting1 Publication7

Domaini

The transmembrane plays critical roles in migration to the peroxisome and/or subsequent insertion into the membrane.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF5T. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42564.
KOiK00434.
OMAiAHNLGRC.
OrthoDBiEOG09360K6Y.
PhylomeDBiQ42564.

Family and domain databases

InterProiView protein in InterPro
IPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
PfamiView protein in Pfam
PF00141. peroxidase. 1 hit.
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiView protein in PROSITE
PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPIVDAEY LKEITKARRE LRSLIANKNC APIMLRLAWH DAGTYDAQSK
60 70 80 90 100
TGGPNGSIRN EEEHTHGANS GLKIALDLCE GVKAKHPKIT YADLYQLAGV
110 120 130 140 150
VAVEVTGGPD IVFVPGRKDS NVCPKEGRLP DAKQGFQHLR DVFYRMGLSD
160 170 180 190 200
KDIVALSGGH TLGRAHPERS GFDGPWTQEP LKFDNSYFVE LLKGESEGLL
210 220 230 240 250
KLPTDKTLLE DPEFRRLVEL YAKDEDAFFR DYAESHKKLS ELGFNPNSSA
260 270 280
GKAVADSTIL AQSAFGVAVA AAVVAFGYFY EIRKRMK
Length:287
Mass (Da):31,572
Last modified:November 1, 1996 - v1
Checksum:iB348E74BA34115DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182K → N in CAA06823 (Ref. 4) Curated1
Sequence conflicti189V → VR in CAA06823 (Ref. 4) Curated1
Sequence conflicti223Missing in CAA06823 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98003 mRNA. Translation: CAA66640.1.
X98276 mRNA. Translation: CAA66926.1.
U69138 mRNA. Translation: AAB71493.1.
AJ006030 Genomic DNA. Translation: CAA06823.1.
AL022023 Genomic DNA. Translation: CAA17765.1.
AL161586 Genomic DNA. Translation: CAB80217.1.
CP002687 Genomic DNA. Translation: AEE86445.1.
AY065143 mRNA. Translation: AAL38319.1.
AY081646 mRNA. Translation: AAM10208.1.
AY086162 mRNA. Translation: AAM63367.1.
PIRiS71279.
RefSeqiNP_195226.1. NM_119666.4.
UniGeneiAt.47586.

Genome annotation databases

EnsemblPlantsiAT4G35000.1; AT4G35000.1; AT4G35000.
GeneIDi829652.
GrameneiAT4G35000.1; AT4G35000.1; AT4G35000.
KEGGiath:AT4G35000.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98003 mRNA. Translation: CAA66640.1.
X98276 mRNA. Translation: CAA66926.1.
U69138 mRNA. Translation: AAB71493.1.
AJ006030 Genomic DNA. Translation: CAA06823.1.
AL022023 Genomic DNA. Translation: CAA17765.1.
AL161586 Genomic DNA. Translation: CAB80217.1.
CP002687 Genomic DNA. Translation: AEE86445.1.
AY065143 mRNA. Translation: AAL38319.1.
AY081646 mRNA. Translation: AAM10208.1.
AY086162 mRNA. Translation: AAM63367.1.
PIRiS71279.
RefSeqiNP_195226.1. NM_119666.4.
UniGeneiAt.47586.

3D structure databases

ProteinModelPortaliQ42564.
SMRiQ42564.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14934. 3 interactors.
STRINGi3702.AT4G35000.1.

Protein family/group databases

PeroxiBasei1891. AtAPx03.

Proteomic databases

PaxDbiQ42564.
PRIDEiQ42564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G35000.1; AT4G35000.1; AT4G35000.
GeneIDi829652.
GrameneiAT4G35000.1; AT4G35000.1; AT4G35000.
KEGGiath:AT4G35000.

Organism-specific databases

AraportiAT4G35000.
TAIRilocus:2131586. AT4G35000.

Phylogenomic databases

eggNOGiENOG410IF5T. Eukaryota.
COG0376. LUCA.
HOGENOMiHOG000189824.
InParanoidiQ42564.
KOiK00434.
OMAiAHNLGRC.
OrthoDBiEOG09360K6Y.
PhylomeDBiQ42564.

Enzyme and pathway databases

BioCyciARA:AT4G35000-MONOMER.

Miscellaneous databases

PROiPR:Q42564.

Gene expression databases

GenevisibleiQ42564. AT.

Family and domain databases

InterProiView protein in InterPro
IPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
PfamiView protein in Pfam
PF00141. peroxidase. 1 hit.
PRINTSiPR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.
SUPFAMiSSF48113. SSF48113. 1 hit.
PROSITEiView protein in PROSITE
PS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAPX3_ARATH
AccessioniPrimary (citable) accession number: Q42564
Secondary accession number(s): O81810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: June 7, 2017
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.