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Protein

L-ascorbate peroxidase 3

Gene

APX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a key role in hydrogen peroxide removal.By similarity

Miscellaneous

Binds one cation per subunit; probably K+, but might also be Ca2+.By similarity

Catalytic activityi

2 L-ascorbate + H2O2 + 2 H+ = L-ascorbate + L-dehydroascorbate + 2 H2O.

Cofactori

heme bNote: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Transition state stabilizerPROSITE-ProRule annotation1
Active sitei40Proton acceptorPROSITE-ProRule annotation1
Metal bindingi160Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi161Potassium or calciumBy similarity1
Metal bindingi177Potassium or calciumBy similarity1
Metal bindingi184Potassium or calciumBy similarity1

GO - Molecular functioni

  • cytochrome-c peroxidase activity Source: GO_Central
  • heme binding Source: InterPro
  • L-ascorbate peroxidase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to oxidative stress Source: GO_Central
  • hydrogen peroxide catabolic process Source: GO_Central
  • response to cytokinin Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to reactive oxygen species Source: GO_Central

Keywordsi

Molecular functionOxidoreductase, Peroxidase
Biological processHydrogen peroxide
LigandCalcium, Heme, Iron, Metal-binding, Potassium

Enzyme and pathway databases

BioCyciARA:AT4G35000-MONOMER

Protein family/group databases

PeroxiBasei1891 AtAPx03

Names & Taxonomyi

Protein namesi
Recommended name:
L-ascorbate peroxidase 3 (EC:1.11.1.11)
Short name:
AtAPx03
Gene namesi
Name:APX3
Synonyms:APX, APXIII
Ordered Locus Names:At4g35000
ORF Names:M4E13.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G35000
TAIRilocus:2131586 AT4G35000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Glyoxysome, Membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002613232 – 287L-ascorbate peroxidase 3Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ42564
PRIDEiQ42564

PTM databases

iPTMnetiQ42564

Expressioni

Gene expression databases

ExpressionAtlasiQ42564 baseline and differential
GenevisibleiQ42564 AT

Interactioni

Subunit structurei

Interacts via its C-terminal region with AKR2A and AKR2B.2 Publications

Protein-protein interaction databases

BioGridi14934, 3 interactors
STRINGi3702.AT4G35000.1

Structurei

3D structure databases

ProteinModelPortaliQ42564
SMRiQ42564
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi281 – 287AKR2A-binding sequence (ABS) required for peroxisome membrane targeting1 Publication7

Domaini

The transmembrane plays critical roles in migration to the peroxisome and/or subsequent insertion into the membrane.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF5T Eukaryota
COG0376 LUCA
HOGENOMiHOG000189824
InParanoidiQ42564
KOiK00434
OMAiHTHGANS
OrthoDBiEOG09360K6Y
PhylomeDBiQ42564

Family and domain databases

InterProiView protein in InterPro
IPR010255 Haem_peroxidase
IPR002016 Haem_peroxidase_pln/fun/bac
IPR002207 Peroxidase_I
IPR019794 Peroxidases_AS
IPR019793 Peroxidases_heam-ligand_BS
PfamiView protein in Pfam
PF00141 peroxidase, 1 hit
PRINTSiPR00459 ASPEROXIDASE
PR00458 PEROXIDASE
SUPFAMiSSF48113 SSF48113, 1 hit
PROSITEiView protein in PROSITE
PS00435 PEROXIDASE_1, 1 hit
PS00436 PEROXIDASE_2, 1 hit
PS50873 PEROXIDASE_4, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42564-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPIVDAEY LKEITKARRE LRSLIANKNC APIMLRLAWH DAGTYDAQSK
60 70 80 90 100
TGGPNGSIRN EEEHTHGANS GLKIALDLCE GVKAKHPKIT YADLYQLAGV
110 120 130 140 150
VAVEVTGGPD IVFVPGRKDS NVCPKEGRLP DAKQGFQHLR DVFYRMGLSD
160 170 180 190 200
KDIVALSGGH TLGRAHPERS GFDGPWTQEP LKFDNSYFVE LLKGESEGLL
210 220 230 240 250
KLPTDKTLLE DPEFRRLVEL YAKDEDAFFR DYAESHKKLS ELGFNPNSSA
260 270 280
GKAVADSTIL AQSAFGVAVA AAVVAFGYFY EIRKRMK
Length:287
Mass (Da):31,572
Last modified:November 1, 1996 - v1
Checksum:iB348E74BA34115DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti182K → N in CAA06823 (Ref. 4) Curated1
Sequence conflicti189V → VR in CAA06823 (Ref. 4) Curated1
Sequence conflicti223Missing in CAA06823 (Ref. 4) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X98003 mRNA Translation: CAA66640.1
X98276 mRNA Translation: CAA66926.1
U69138 mRNA Translation: AAB71493.1
AJ006030 Genomic DNA Translation: CAA06823.1
AL022023 Genomic DNA Translation: CAA17765.1
AL161586 Genomic DNA Translation: CAB80217.1
CP002687 Genomic DNA Translation: AEE86445.1
AY065143 mRNA Translation: AAL38319.1
AY081646 mRNA Translation: AAM10208.1
AY086162 mRNA Translation: AAM63367.1
PIRiS71279
RefSeqiNP_195226.1, NM_119666.4
UniGeneiAt.47586

Genome annotation databases

EnsemblPlantsiAT4G35000.1; AT4G35000.1; AT4G35000
GeneIDi829652
GrameneiAT4G35000.1; AT4G35000.1; AT4G35000
KEGGiath:AT4G35000

Similar proteinsi

Entry informationi

Entry nameiAPX3_ARATH
AccessioniPrimary (citable) accession number: Q42564
Secondary accession number(s): O81810
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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