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Protein

Adenine phosphoribosyltransferase 2

Gene

APT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. May contribute to the recycling of adenine into adenylate nucleotides and the inactivation of cytokinins by phosphoribosylation. Possesses low activity toward adenine and cytokinins.2 Publications

Catalytic activityi

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.2 Publications

Kineticsi

  1. KM=2.6 µM for adenine (at pH 7.4 and 37 degrees Celsius)1 Publication
  2. KM=330 µM for zeatin (at pH 7.4 and 37 degrees Celsius)1 Publication
  3. KM=110 µM for isopentenyladenine (at pH 7.4 and 37 degrees Celsius)1 Publication
  4. KM=15 µM for benzyladenine (at pH 7.4 and 37 degrees Celsius)1 Publication
  1. Vmax=0.31 µmol/min/mg enzyme with adenine as substrate (at pH 7.4 and 37 degrees Celsius)1 Publication
  2. Vmax=1 µmol/min/mg enzyme with zeatin as substrate (at pH 7.4 and 37 degrees Celsius)1 Publication
  3. Vmax=0.36 µmol/min/mg enzyme with isopentenyladenine as substrate (at pH 7.4 and 37 degrees Celsius)1 Publication
  4. Vmax=0.1 µmol/min/mg enzyme with benzyladenine as substrate (at pH 7.4 and 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 7.4-7.5.1 Publication

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenine phosphoribosyltransferase 3 (APT3), Adenine phosphoribosyltransferase 1, chloroplastic (APT1), Adenine phosphoribosyltransferase 5 (APT5), Adenine phosphoribosyltransferase 4 (APT4), Adenine phosphoribosyltransferase 2 (APT2)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

GO - Molecular functioni

  • adenine phosphoribosyltransferase activity Source: TAIR
  • phosphate ion transmembrane transporter activity Source: TAIR

GO - Biological processi

  • adenine salvage Source: TAIR
  • AMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciARA:AT1G80050-MONOMER.
ReactomeiR-ATH-74217. Purine salvage.
UniPathwayiUPA00588; UER00646.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine phosphoribosyltransferase 2 (EC:2.4.2.7)
Short name:
APRT 2
Short name:
AtAPT2
Gene namesi
Name:APT2
Ordered Locus Names:At1g80050
ORF Names:F18B13.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G80050.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: TAIR
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Adenine phosphoribosyltransferase 2PRO_0000149515Add
BLAST

Proteomic databases

PaxDbiQ42563.
PRIDEiQ42563.

Expressioni

Gene expression databases

GenevisibleiQ42563. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi29563. 2 interactions.
IntActiQ42563. 2 interactions.
STRINGi3702.AT1G80050.1.

Structurei

3D structure databases

ProteinModelPortaliQ42563.
SMRiQ42563. Positions 13-174.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
HOGENOMiHOG000036776.
InParanoidiQ42563.
KOiK00759.
OMAiLEMSVEA.
PhylomeDBiQ42563.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42563-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAVENGLQG DPRLKAISDA IRVIPHFPKT GIMFQDITTL LLDPVAFKHV
60 70 80 90 100
VDIFVDRYKH MNISLVAGVE ARGFIFGPPI ALAIGAKFVP LRKPGKLPGR
110 120 130 140 150
VISEEYELEY GRDCLEMSVE AVKSEERALI IDDLVATGGT LSASINLLER
160 170 180 190
AGAEVVECAC VVGLPKFKGQ CKLKGKPLYV LVEPNQFDEL TL
Length:192
Mass (Da):21,009
Last modified:November 1, 1997 - v1
Checksum:i29D648EE59BBB026
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401L → M in CAA65610 (PubMed:8696367).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96866 mRNA. Translation: CAA65609.1.
X96867 Genomic DNA. Translation: CAA65610.1.
AC009322 Genomic DNA. Translation: AAD55485.1.
CP002684 Genomic DNA. Translation: AEE36351.1.
AY072021 mRNA. Translation: AAL57714.1.
BT001122 mRNA. Translation: AAN64513.1.
PIRiS71272.
RefSeqiNP_178122.1. NM_106654.2.
UniGeneiAt.130.

Genome annotation databases

EnsemblPlantsiAT1G80050.1; AT1G80050.1; AT1G80050.
GeneIDi844345.
GrameneiAT1G80050.1; AT1G80050.1; AT1G80050.
KEGGiath:AT1G80050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X96866 mRNA. Translation: CAA65609.1.
X96867 Genomic DNA. Translation: CAA65610.1.
AC009322 Genomic DNA. Translation: AAD55485.1.
CP002684 Genomic DNA. Translation: AEE36351.1.
AY072021 mRNA. Translation: AAL57714.1.
BT001122 mRNA. Translation: AAN64513.1.
PIRiS71272.
RefSeqiNP_178122.1. NM_106654.2.
UniGeneiAt.130.

3D structure databases

ProteinModelPortaliQ42563.
SMRiQ42563. Positions 13-174.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29563. 2 interactions.
IntActiQ42563. 2 interactions.
STRINGi3702.AT1G80050.1.

Proteomic databases

PaxDbiQ42563.
PRIDEiQ42563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G80050.1; AT1G80050.1; AT1G80050.
GeneIDi844345.
GrameneiAT1G80050.1; AT1G80050.1; AT1G80050.
KEGGiath:AT1G80050.

Organism-specific databases

TAIRiAT1G80050.

Phylogenomic databases

eggNOGiKOG1712. Eukaryota.
COG0503. LUCA.
HOGENOMiHOG000036776.
InParanoidiQ42563.
KOiK00759.
OMAiLEMSVEA.
PhylomeDBiQ42563.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00646.
BioCyciARA:AT1G80050-MONOMER.
ReactomeiR-ATH-74217. Purine salvage.

Miscellaneous databases

PROiQ42563.

Gene expression databases

GenevisibleiQ42563. AT.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_00004. Aden_phosphoribosyltr.
InterProiIPR005764. Ade_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01090. apt. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A second form of adenine phosphoribosyltransferase in Arabidopsis thaliana with relative specificity towards cytokinins."
    Schnorr K.M., Gaillard C., Biget E., Nygaard P., Laloue M.
    Plant J. 9:891-898(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Adenine phosphoribosyltransferase isoforms of Arabidopsis and their potential contributions to adenine and cytokinin metabolism."
    Allen M., Qin W., Moreau F., Moffatt B.
    Physiol. Plantarum 115:56-68(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
  6. "Adenine phosphoribosyl transferase 1 is a key enzyme catalyzing cytokinin conversion from nucleobases to nucleotides in Arabidopsis."
    Zhang X., Chen Y., Lin X., Hong X., Zhu Y., Li W., He W., An F., Guo H.
    Mol. Plant 6:1661-1672(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.

Entry informationi

Entry nameiAPT2_ARATH
AccessioniPrimary (citable) accession number: Q42563
Secondary accession number(s): Q39004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.