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Q42556 (PMA9_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATPase 9, plasma membrane-type
EC=3.6.3.6
Alternative name(s):
Proton pump 9
Gene names
Name:AHA9
Ordered Locus Names:At1g80660
ORF Names:F23A5.1, T21F11.1
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length954 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activity

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Subunit structure

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-953. Binding to 14-3-3 proteins activates the H+-ATPase By similarity.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Anther specific.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIIA subfamily. [View classification]

Sequence caution

The sequence AAA98916.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42556-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 954954ATPase 9, plasma membrane-type
PRO_0000046282

Regions

Topological domain1 – 6666Cytoplasmic Potential
Transmembrane67 – 8620Helical; Name=1; Potential
Topological domain87 – 9812Extracellular Potential
Transmembrane99 – 11921Helical; Name=2; Potential
Topological domain120 – 248129Cytoplasmic Potential
Transmembrane249 – 26921Helical; Name=3; Potential
Topological domain270 – 2789Extracellular Potential
Transmembrane279 – 29618Helical; Name=4; Potential
Topological domain297 – 648352Cytoplasmic Potential
Transmembrane649 – 67022Helical; Name=5; Potential
Topological domain671 – 6755Extracellular Potential
Transmembrane676 – 69823Helical; Name=6; Potential
Topological domain699 – 71416Cytoplasmic Potential
Transmembrane715 – 73521Helical; Name=7; Potential
Topological domain736 – 75621Extracellular Potential
Transmembrane757 – 77721Helical; Name=8; Potential
Topological domain778 – 78912Cytoplasmic Potential
Transmembrane790 – 81021Helical; Name=9; Potential
Topological domain811 – 8188Extracellular Potential
Transmembrane819 – 83921Helical; Name=10; Potential
Topological domain840 – 954115Cytoplasmic Potential
Region952 – 9543Interaction with 14-3-3 proteins By similarity

Sites

Active site33414-aspartylphosphate intermediate By similarity
Metal binding5931Magnesium By similarity
Metal binding5971Magnesium By similarity

Amino acid modifications

Modified residue9531Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 34DFCB96F45E5F18

FASTA954105,208
        10         20         30         40         50         60 
MAGNKDSSWD DIKNEGIDLE KIPIEEVLTQ LRCTREGLTS DEGQTRLEIF GPNKLEEKKE 

        70         80         90        100        110        120 
NKVLKFLGFM WNPLSWVMEL AAIMAIALAN GGGRPPDWQD FVGITVLLII NSTISFIEEN 

       130        140        150        160        170        180 
NAGNAAAALM AGLAPKTKVL RDGKWSEQEA AILVPGDIIS IKLGDIVPAD GRLLDGDPLK 

       190        200        210        220        230        240 
IDQSALTGES LPVTKHPGQE VYSGSTCKQG ELEAVVIATG VHTFFGKAAH LVDSTNQEGH 

       250        260        270        280        290        300 
FQKVLTAIGN FCICSIAIGM LIEIVVMYPI QKRAYRDGID NLLVLLIGGI PIAMPTVLSV 

       310        320        330        340        350        360 
TMAIGSHRLS QQGAITKRMT AIEEMAGMDV LCSDKTGTLT LNKLTVDKSM VEVFVKDLDK 

       370        380        390        400        410        420 
DQLLVNAARA SRVENQDAID ACIVGMLGDP REAREGITEV HFFPFNPVDK RTAITYIDAN 

       430        440        450        460        470        480 
GNWHRVSKGA PEQIIELCNL REDASKRAHD IIDKFADRGL RSLAVGRQTV SEKDKNSPGE 

       490        500        510        520        530        540 
PWQFLGLLPL FDPPRHDSAE TIRRALDLGV NVKMITGDQL AIGKETGRRL GMGTNMYPSS 

       550        560        570        580        590        600 
ALLGQDKDES IASLPVDELI EKADGFAGVF PEHKYEIVKR LQEMKHICGM TGDGVNDAPA 

       610        620        630        640        650        660 
LKRADIGIAV ADATDAARSA SDIVLTEPGL SVIVSAVLTS RAIFQRMKNY TIYAVSITIR 

       670        680        690        700        710        720 
IVMGFMLLAL IWKFDFSPFM VLIVAILNDG TIMTISKDRV KPSPLPDSWK LKEIFATGVV 

       730        740        750        760        770        780 
LGTYLAVMTV VFFWAAESTD FFSAKFGVRS ISGNPHELTA AVYLQVSIVS QALIFVTRSR 

       790        800        810        820        830        840 
SWSYVERPGF WLISAFFMAQ LIATLIAVYA NWNFARIRGI GWGWAGVIWL YSIVFYIPLD 

       850        860        870        880        890        900 
ILKFIIRYSL SGRAWDNVIE NKTAFTSKKD YGKGEREAQW AQAQRTLHGL QPAQTSDMFN 

       910        920        930        940        950 
DKSTYRELSE IADQAKRRAE VARLRERHTL KGHVESVVKQ KGLDIEAIQQ HYTL

« Hide

References

« Hide 'large scale' references
[1]"Identification of an Arabidopsis thaliana gene encoding a plasma membrane H(+)-ATPase whose expression is restricted to anther tissue."
Houlne G., Boutry M.
Plant J. 5:311-317(1994) [PubMed: 8180619] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"A 37.5 Kb sequence from Arabidopsis thaliana chromosome I."
Goodman H.M., Gallant P., Keifer-Higgins S., Rubenfield M., Church G.M.
Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[3]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X73676 Genomic DNA. No translation available.
U53501 Genomic DNA. Translation: AAA98916.1. Sequence problems.
AC011713 Genomic DNA. Translation: AAF14653.1.
AC018849 Genomic DNA. Translation: AAF27113.1.
CP002684 Genomic DNA. Translation: AEE36433.1.
IPIIPI00530662.
PIRH96838.
S60301.
RefSeqNP_178181.1. NM_106714.1.
UniGeneAt.52582.

3D structure databases

ProteinModelPortalQ42556.
SMRQ42556. Positions 17-848, 925-954.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ42556.

Proteomic databases

PRIDEQ42556.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G80660.1; AT1G80660.1; AT1G80660.
GeneID844405.
GenomeReviewsGene locus AT1G80660 in contig CT485782_GR.
KEGGath:AT1G80660.
NMPDRfig|3702.1.peg.7657.

Organism-specific databases

TAIRAt1g80660.

Phylogenomic databases

GeneTreeEPGT00050000005325.
HOGENOMHBG706356.
InParanoidQ42556.
OMANTNISAG.
PhylomeDBQ42556.

Gene expression databases

ArrayExpressQ42556.
GenevestigatorQ42556.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-dom.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023300. ATPase_P-typ_cyto_domA.
IPR023299. ATPase_P-typ_cyto_domN.
IPR000695. ATPase_P-typ_H-transp.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006534. ATPase_P-typ_PM_proton-efflux.
IPR023298. ATPase_P-typ_TM_dom.
IPR005834. Dehalogen-like_hydro.
IPR023214. HAD-like_dom.
[Graphical view]
Gene3DG3DSA:2.70.150.10. ATPase_P-typ_cyto_domA. 2 hits.
G3DSA:3.40.1110.10. ATPase_P-typ_cyto_domN. 1 hit.
G3DSA:1.20.1110.10. ATPase_P-typ_TM_dom. 3 hits.
KOK01535.
PANTHERPTHR24093:SF61. PTHR24093:SF61. 1 hit.
PfamPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. HAD-like_dom. 1 hit.
TIGRFAMsTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMA9_ARATH
AccessionPrimary (citable) accession number: Q42556
Secondary accession number(s): Q9M8N3, Q9SAJ1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: November 16, 2011
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents