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Protein

ATPase 9, plasma membrane-type

Gene

AHA9

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The plasma membrane H+ ATPase of plants and fungi generates a proton gradient that drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei334 – 33414-aspartylphosphate intermediateBy similarity
Metal bindingi593 – 5931MagnesiumBy similarity
Metal bindingi597 – 5971MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G80660-MONOMER.
ARA:GQT-2573-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase 9, plasma membrane-type (EC:3.6.3.6)
Alternative name(s):
Proton pump 9
Gene namesi
Name:AHA9
Ordered Locus Names:At1g80660
ORF Names:F23A5.1, T21F11.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G80660.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6666CytoplasmicSequence analysisAdd
BLAST
Transmembranei67 – 8620Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini87 – 9812ExtracellularSequence analysisAdd
BLAST
Transmembranei99 – 11921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini120 – 248129CytoplasmicSequence analysisAdd
BLAST
Transmembranei249 – 26921Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini270 – 2789ExtracellularSequence analysis
Transmembranei279 – 29618Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini297 – 648352CytoplasmicSequence analysisAdd
BLAST
Transmembranei649 – 67022Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini671 – 6755ExtracellularSequence analysis
Transmembranei676 – 69823Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini699 – 71416CytoplasmicSequence analysisAdd
BLAST
Transmembranei715 – 73521Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini736 – 75621ExtracellularSequence analysisAdd
BLAST
Transmembranei757 – 77721Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini778 – 78912CytoplasmicSequence analysisAdd
BLAST
Transmembranei790 – 81021Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini811 – 8188ExtracellularSequence analysis
Transmembranei819 – 83921Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini840 – 954115CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 954954ATPase 9, plasma membrane-typePRO_0000046282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei886 – 8861PhosphothreonineBy similarity
Modified residuei936 – 9361PhosphoserineBy similarity
Modified residuei953 – 9531PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ42556.
PRIDEiQ42556.

PTM databases

iPTMnetiQ42556.

Expressioni

Tissue specificityi

Anther specific.

Gene expression databases

ExpressionAtlasiQ42556. baseline and differential.
GenevisibleiQ42556. AT.

Interactioni

Subunit structurei

Binds to 14-3-3 proteins. The binding is induced by phosphorylation of Thr-953. Binding to 14-3-3 proteins activates the H+-ATPase (By similarity).By similarity

Protein-protein interaction databases

BioGridi29623. 1 interaction.
STRINGi3702.AT1G80660.1.

Structurei

3D structure databases

ProteinModelPortaliQ42556.
SMRiQ42556. Positions 17-848, 925-954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni952 – 9543Interaction with 14-3-3 proteinsBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ42556.
KOiK01535.
OMAiIEILVMY.
PhylomeDBiQ42556.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42556-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGNKDSSWD DIKNEGIDLE KIPIEEVLTQ LRCTREGLTS DEGQTRLEIF
60 70 80 90 100
GPNKLEEKKE NKVLKFLGFM WNPLSWVMEL AAIMAIALAN GGGRPPDWQD
110 120 130 140 150
FVGITVLLII NSTISFIEEN NAGNAAAALM AGLAPKTKVL RDGKWSEQEA
160 170 180 190 200
AILVPGDIIS IKLGDIVPAD GRLLDGDPLK IDQSALTGES LPVTKHPGQE
210 220 230 240 250
VYSGSTCKQG ELEAVVIATG VHTFFGKAAH LVDSTNQEGH FQKVLTAIGN
260 270 280 290 300
FCICSIAIGM LIEIVVMYPI QKRAYRDGID NLLVLLIGGI PIAMPTVLSV
310 320 330 340 350
TMAIGSHRLS QQGAITKRMT AIEEMAGMDV LCSDKTGTLT LNKLTVDKSM
360 370 380 390 400
VEVFVKDLDK DQLLVNAARA SRVENQDAID ACIVGMLGDP REAREGITEV
410 420 430 440 450
HFFPFNPVDK RTAITYIDAN GNWHRVSKGA PEQIIELCNL REDASKRAHD
460 470 480 490 500
IIDKFADRGL RSLAVGRQTV SEKDKNSPGE PWQFLGLLPL FDPPRHDSAE
510 520 530 540 550
TIRRALDLGV NVKMITGDQL AIGKETGRRL GMGTNMYPSS ALLGQDKDES
560 570 580 590 600
IASLPVDELI EKADGFAGVF PEHKYEIVKR LQEMKHICGM TGDGVNDAPA
610 620 630 640 650
LKRADIGIAV ADATDAARSA SDIVLTEPGL SVIVSAVLTS RAIFQRMKNY
660 670 680 690 700
TIYAVSITIR IVMGFMLLAL IWKFDFSPFM VLIVAILNDG TIMTISKDRV
710 720 730 740 750
KPSPLPDSWK LKEIFATGVV LGTYLAVMTV VFFWAAESTD FFSAKFGVRS
760 770 780 790 800
ISGNPHELTA AVYLQVSIVS QALIFVTRSR SWSYVERPGF WLISAFFMAQ
810 820 830 840 850
LIATLIAVYA NWNFARIRGI GWGWAGVIWL YSIVFYIPLD ILKFIIRYSL
860 870 880 890 900
SGRAWDNVIE NKTAFTSKKD YGKGEREAQW AQAQRTLHGL QPAQTSDMFN
910 920 930 940 950
DKSTYRELSE IADQAKRRAE VARLRERHTL KGHVESVVKQ KGLDIEAIQQ

HYTL
Length:954
Mass (Da):105,208
Last modified:January 11, 2001 - v2
Checksum:i34DFCB96F45E5F18
GO

Sequence cautioni

The sequence AAA98916.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73676 Genomic DNA. No translation available.
U53501 Genomic DNA. Translation: AAA98916.1. Sequence problems.
AC011713 Genomic DNA. Translation: AAF14653.1.
AC018849 Genomic DNA. Translation: AAF27113.1.
CP002684 Genomic DNA. Translation: AEE36433.1.
PIRiH96838.
S60301.
RefSeqiNP_178181.1. NM_106714.1. [Q42556-1]
UniGeneiAt.52582.

Genome annotation databases

EnsemblPlantsiAT1G80660.1; AT1G80660.1; AT1G80660. [Q42556-1]
GeneIDi844405.
KEGGiath:AT1G80660.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X73676 Genomic DNA. No translation available.
U53501 Genomic DNA. Translation: AAA98916.1. Sequence problems.
AC011713 Genomic DNA. Translation: AAF14653.1.
AC018849 Genomic DNA. Translation: AAF27113.1.
CP002684 Genomic DNA. Translation: AEE36433.1.
PIRiH96838.
S60301.
RefSeqiNP_178181.1. NM_106714.1. [Q42556-1]
UniGeneiAt.52582.

3D structure databases

ProteinModelPortaliQ42556.
SMRiQ42556. Positions 17-848, 925-954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi29623. 1 interaction.
STRINGi3702.AT1G80660.1.

PTM databases

iPTMnetiQ42556.

Proteomic databases

PaxDbiQ42556.
PRIDEiQ42556.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G80660.1; AT1G80660.1; AT1G80660. [Q42556-1]
GeneIDi844405.
KEGGiath:AT1G80660.

Organism-specific databases

TAIRiAT1G80660.

Phylogenomic databases

eggNOGiKOG0205. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000160005.
InParanoidiQ42556.
KOiK01535.
OMAiIEILVMY.
PhylomeDBiQ42556.

Enzyme and pathway databases

BioCyciARA:AT1G80660-MONOMER.
ARA:GQT-2573-MONOMER.

Miscellaneous databases

PROiQ42556.

Gene expression databases

ExpressionAtlasiQ42556. baseline and differential.
GenevisibleiQ42556. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of an Arabidopsis thaliana gene encoding a plasma membrane H(+)-ATPase whose expression is restricted to anther tissue."
    Houlne G., Boutry M.
    Plant J. 5:311-317(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  2. "A 37.5 Kb sequence from Arabidopsis thaliana chromosome I."
    Goodman H.M., Gallant P., Keifer-Higgins S., Rubenfield M., Church G.M.
    Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiPMA9_ARATH
AccessioniPrimary (citable) accession number: Q42556
Secondary accession number(s): Q9M8N3, Q9SAJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: June 8, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.