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Protein

SUMO-conjugating enzyme SCE1

Gene

SCE1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SUMO-conjugating enzyme that accepts the SUMO proteins from the E1 SUMO-activating heterodimer SAE1/SAE2 and catalyzes its covalent attachment to other proteins with the E3 SUMO ligases SIZ1 and MMS21. Associates with SIZ1 for sumoylation of the transcription factor GTE3.3 Publications

Catalytic activityi

ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei94Glycyl thioester intermediate1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • SUMO transferase activity Source: TAIR

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • protein sumoylation Source: TAIR
  • response to abscisic acid Source: TAIR

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00886

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzyme SCE1 (EC:2.3.2.-)
Alternative name(s):
Protein EMBRYO DEFECTIVE 1637
Protein hus5 homolog
SUMO-conjugating enzyme 1
Short name:
AtSCE1
Gene namesi
Name:SCE1
Synonyms:AHUS5, EMB1637
Ordered Locus Names:At3g57870
ORF Names:T10K17.80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G57870
TAIRilocus:2095838 AT3G57870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi94C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00003960132 – 160SUMO-conjugating enzyme SCE1Add BLAST159

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ42551

Expressioni

Gene expression databases

ExpressionAtlasiQ42551 baseline and differential
GenevisibleiQ42551 AT

Interactioni

Subunit structurei

Interacts with SIZ1 (via PHD domain) and MMS21.2 Publications

Protein-protein interaction databases

BioGridi10271, 165 interactors
IntActiQ42551, 5 interactors
STRINGi3702.AT3G57870.1

Structurei

3D structure databases

ProteinModelPortaliQ42551
SMRiQ42551
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0424 Eukaryota
COG5078 LUCA
HOGENOMiHOG000233454
InParanoidiQ42551
KOiK10577
OMAiKCKFTPP
OrthoDBiEOG09360OGE
PhylomeDBiQ42551

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR027230 Ubc9
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PANTHERiPTHR43927 PTHR43927, 1 hit
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42551-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGIARGRL AEERKSWRKN HPHGFVAKPE TGQDGTVNLM VWHCTIPGKA
60 70 80 90 100
GTDWEGGFFP LTMHFSEDYP SKPPKCKFPQ GFFHPNVYPS GTVCLSILNE
110 120 130 140 150
DYGWRPAITV KQILVGIQDL LDTPNPADPA QTDGYHLFCQ DPVEYKKRVK
160
LQSKQYPALV
Length:160
Mass (Da):17,986
Last modified:November 1, 1996 - v1
Checksum:i6D9E5C538B09D345
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157P → A in BAF01688 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44976 mRNA Translation: AAA86642.1
AF091106 Genomic DNA Translation: AAC64116.1
AL132977 Genomic DNA Translation: CAB67615.1
CP002686 Genomic DNA Translation: AEE79711.1
AY042838 mRNA Translation: AAK68778.1
BT003377 mRNA Translation: AAO30040.1
AK229859 mRNA Translation: BAF01688.1
PIRiT46009
RefSeqiNP_191346.1, NM_115649.3
UniGeneiAt.21103
At.21178
At.75366

Genome annotation databases

EnsemblPlantsiAT3G57870.1; AT3G57870.1; AT3G57870
GeneIDi824956
GrameneiAT3G57870.1; AT3G57870.1; AT3G57870
KEGGiath:AT3G57870

Similar proteinsi

Entry informationi

Entry nameiSCE1_ARATH
AccessioniPrimary (citable) accession number: Q42551
Secondary accession number(s): Q0WMG8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 10, 2010
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health