SAL1 phosphatase - Arabidopsis thaliana (Mouse-ear cress)

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Q42546 (DPNP1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
SAL1 phosphatase

Alternative name(s):
3'(2'),5'-bisphosphate nucleotidase 1
EC=3.1.3.7
3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase 1
DPNPase 1
Inositol polyphosphate 1-phosphatase 1
Short name=IPPase 1
Inositol-1,4-bisphosphate 1-phosphatase 1
EC=3.1.3.57
Protein FIERY 1

Gene names

Name:	SAL1
Synonyms:	FRY1
Ordered Locus Names:	At5g63980
ORF Names:	MBM17.8

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. May play a role in the biosynthesis of sulfate conjugates and RNA processing. Is also able to hydrolyze inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate. Could be considered as a negative regulator of abscisic acid (ABA)- and stress-responsive genes, through modulating the inositol 1,4,5-trisphosphate (IP3) turnover. Is also involved in salt tolerance. Acts as a suppressor of virus- and transgene-induced silencing. Ref.1 Ref.2 Ref.6
Catalytic activity	Adenosine 3',5'-bisphosphate + H₂O = adenosine 5'-phosphate + phosphate. 1D-myo-inositol 1,4-bisphosphate + H₂O = 1D-myo-inositol 4-phosphate + phosphate.
Cofactor	Magnesium.
Enzyme regulation	Inhibited by Li⁺ (IC₅₀=0.20 millimolar) and Na⁺ (IC₅₀=200 millimolar).
Pathway	Signal transduction; phosphatidylinositol signaling pathway.
Tissue specificity	Expressed in roots, leaves, stems, flowers and siliques. Ref.2 Ref.5
Miscellaneous	Mutation in the FRY1 gene results in super-induction of abscisic acid (ABA)- and stress-responsive genes, due to inositol 1,4,5-trisphosphate (IP3) accumulation. Substrate preference is 3'-phosphoadenosine 5'-phosphate (PAP) = adenosine 3'-phosphate 5'-phosphosulfate (PAPS)> inositol-1,4-bisphosphate >> inositol 1,4,5-trisphosphate. No activity observed against 3' or 5'-AMP, inositol monophosphate and fructose-1,6-bisphosphate.
Sequence similarities	Belongs to the inositol monophosphatase family.

Ontologies

Keywords
Biological process	Abscisic acid signaling pathway
Ligand	Calcium Lithium Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	abscisic acid mediated signaling pathway Inferred from mutant phenotype Ref.2. Source: TAIR miRNA catabolic process Inferred from mutant phenotype Ref.6. Source: TAIR negative regulation of signal transduction Inferred from mutant phenotype. Source: TAIR negative regulation of transcription, DNA-dependent Inferred from expression pattern. Source: TAIR phosphatidylinositol-mediated signaling Traceable author statement Ref.1. Source: TAIR photoperiodism, flowering Inferred from mutant phenotype. Source: TAIR positive regulation of unidimensional cell growth Inferred from mutant phenotype. Source: TAIR regulation of jasmonic acid biosynthetic process Inferred from mutant phenotype. Source: TAIR response to cation stress Inferred from genetic interaction Ref.1. Source: TAIR response to cold Inferred from mutant phenotype. Source: TAIR response to water deprivation Inferred from mutant phenotype. Source: TAIR sulfur compound metabolic process Inferred from genetic interaction Ref.1. Source: TAIR
Cellular component	chloroplast stroma Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR nucleus Inferred from direct assay. Source: TAIR
Molecular function	3'(2'),5'-bisphosphate nucleotidase activity Inferred from direct assay Ref.1. Source: TAIR inositol-1,4-bisphosphate 1-phosphatase activity Inferred from direct assay Ref.1. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide phosphatase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 353

353

SAL1 phosphatase

PRO_0000142530

Regions

Region

136 – 139

Substrate binding By similarity

Sites

Metal binding

Magnesium 1 By similarity

Metal binding

134

Magnesium 1 By similarity

Metal binding

134

Magnesium 2 By similarity

Metal binding

136

Magnesium 1; via carbonyl oxygen By similarity

Metal binding

137

Magnesium 2 By similarity

Metal binding

288

Magnesium 2 By similarity

Binding site

Substrate By similarity

Binding site

288

Substrate By similarity

Experimental info

Mutagenesis

287 – 353

Missing in fry1-1; abolishes activity.

Sequences

Sequence

Length

Mass (Da)

Tools

Q42546 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 351EBB6826B93A4A
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FASTA

353

37,564

        10         20         30         40         50         60 
MAYEKELDAA KKAASLAARL CQKVQKALLQ SDVQSKSDKS PVTVADYGSQ AVVSLVLEKE 

        70         80         90        100        110        120 
LSSEPFSLVA EEDSGDLRKD GSQDTLERIT KLVNDTLATE ESFNGSTLST DDLLRAIDCG 

       130        140        150        160        170        180 
TSEGGPNGRH WVLDPIDGTK GFLRGDQYAV ALGLLEEGKV VLGVLACPNL PLASIAGNNK 

       190        200        210        220        230        240 
NKSSSDEIGC LFFATIGSGT YMQLLDSKSS PVKVQVSSVE NPEEASFFES FEGAHSLHDL 

       250        260        270        280        290        300 
SSSIANKLGV KAPPVRIDSQ AKYGALSRGD GAIYLRFPHK GYREKIWDHV AGAIVVTEAG 

       310        320        330        340        350 
GIVTDAAGKP LDFSKGKYLD LDTGIIVANE KLMPLLLKAV RDSIAEQEKA SAL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The SAL1 gene of Arabidopsis, encoding an enzyme with 3'(2'),5'-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, increases salt tolerance in yeast." Quintero F.J., Garciadeblas B., Rodriguez-Navarro A. Plant Cell 8:529-537(1996) [PubMed: 8721754] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Tissue: Root.
[2]	"FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis." Xiong L., Lee B.-H., Ishitani M., Lee H., Zhang C., Zhu J.-K. Genes Dev. 15:1971-1984(2001) [PubMed: 11485991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, FUNCTION, MUTANT FRY1-1. Strain: cv. C24.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S. DNA Res. 7:31-63(2000) [PubMed: 10718197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The Arabidopsis HAL2-like gene family includes a novel sodium-sensitive phosphatase." Gil-Mascarell R., Lopez-Coronado J.M., Belles J.M., Serrano R., Rodriguez P.L. Plant J. 17:373-383(1999) [PubMed: 10205895] [Abstract] Cited for: TISSUE SPECIFICITY, CHARACTERIZATION. Strain: cv. Columbia.
[6]	"Arabidopsis FIERY1, XRN2, and XRN3 are endogenous RNA silencing suppressors." Gy I., Gasciolli V., Lauressergues D., Morel J.-B., Gombert J., Proux F., Proux C., Vaucheret H., Mallory A.C. Plant Cell 19:3451-3461(2007) [PubMed: 17993620] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40433 mRNA. Translation: AAC49263.1. AY034894 mRNA. Translation: AAK58887.1. AB019227 Genomic DNA. Translation: BAA96901.1. BT005993 mRNA. Translation: AAO64928.1.
IPI	IPI00542330.
3D structure databases
ProteinModelPortal	Q42546.
SMR	Q42546. Positions 2-347.
ModBase	Search...
Protein-protein interaction databases
STRING	Q42546.
Proteomic databases
PRIDE	Q42546.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus AT5G63980 in contig BA000015_GR.
NMPDR	fig\|3702.1.peg.28453.
Organism-specific databases
GeneFarm	2320.
TAIR	At5g63980.
Phylogenomic databases
GeneTree	EPGT00070000029602.
HOGENOM	HBG599916.
InParanoid	Q42546.
OMA	HAAGCIV.
PhylomeDB	Q42546.
Gene expression databases
ArrayExpress	Q42546.
Genevestigator	Q42546.
GermOnline	AT5G63980. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006239. Bisphos_HAL2. IPR020583. Inositol_monoP_metal-BS. IPR000760. Inositol_monophosphatase. [Graphical view]
PANTHER	PTHR20854. Inositol_P. 1 hit.
Pfam	PF00459. Inositol_P. 1 hit. [Graphical view]
PRINTS	PR00377. IMPHPHTASES.
TIGRFAMs	TIGR01330. Bisphos_HAL2. 1 hit.
PROSITE	PS00629. IMP_1. 1 hit. PS00630. IMP_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DPNP1_ARATH

Accession

Primary (citable) accession number: Q42546

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents