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Protein

SAL1 phosphatase

Gene

SAL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts adenosine 3'-phosphate 5'-phosphosulfate (PAPS) to adenosine 5'-phosphosulfate (APS) and 3'(2')-phosphoadenosine 5'- phosphate (PAP) to AMP. May regulate the flux of sulfur in the sulfur-activation pathway by converting PAPS to APS. May play a role in the biosynthesis of sulfate conjugates and RNA processing. Is also able to hydrolyze inositol 1,4-bisphosphate and inositol 1,3,4-trisphosphate. Could be considered as a negative regulator of abscisic acid (ABA)- and stress-responsive genes, through modulating the inositol 1,4,5-trisphosphate (IP3) turnover. Is also involved in salt tolerance. Acts as a suppressor of virus- and transgene-induced silencing.3 Publications

Miscellaneous

Mutation in the FRY1 gene results in super-induction of abscisic acid (ABA)- and stress-responsive genes, due to inositol 1,4,5-trisphosphate (IP3) accumulation.
Substrate preference is 3'-phosphoadenosine 5'-phosphate (PAP) = adenosine 3'-phosphate 5'-phosphosulfate (PAPS)> inositol 1,4-bisphosphate >> inositol 1,4,5-trisphosphate. No activity observed against 3' or 5'-AMP, inositol monophosphate and fructose-1,6-bisphosphate.

Catalytic activityi

Adenosine 3',5'-bisphosphate + H2O = adenosine 5'-phosphate + phosphate.
1D-myo-inositol 1,4-bisphosphate + H2O = 1D-myo-inositol 4-phosphate + phosphate.

Cofactori

Enzyme regulationi

Inhibited by Li+ (IC50=0.20 millimolar) and Na+ (IC50=200 millimolar).

Pathwayi: phosphatidylinositol signaling pathway

This protein is involved in the pathway phosphatidylinositol signaling pathway, which is part of Signal transduction.
View all proteins of this organism that are known to be involved in the pathway phosphatidylinositol signaling pathway and in Signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi71Magnesium 1By similarity1
Binding sitei71SubstrateBy similarity1
Metal bindingi134Magnesium 1By similarity1
Metal bindingi134Magnesium 2By similarity1
Metal bindingi136Magnesium 1; via carbonyl oxygenBy similarity1
Metal bindingi137Magnesium 2By similarity1
Metal bindingi288Magnesium 2By similarity1
Binding sitei288SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme
Biological processAbscisic acid signaling pathway
LigandCalcium, Lithium, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciARA:AT5G63980-MONOMER
BRENDAi3.1.3.31 399
3.1.3.57 399
3.1.3.7 399
UniPathwayiUPA00944

Names & Taxonomyi

Protein namesi
Recommended name:
SAL1 phosphatase
Alternative name(s):
3'(2'),5'-bisphosphate nucleotidase 1 (EC:3.1.3.7)
3'(2'),5'-bisphosphonucleoside 3'(2')-phosphohydrolase 1
DPNPase 1
Inositol polyphosphate 1-phosphatase 1
Short name:
IPPase 1
Inositol-1,4-bisphosphate 1-phosphatase 1 (EC:3.1.3.57)
Protein FIERY 1
Gene namesi
Name:SAL1
Synonyms:FRY1
Ordered Locus Names:At5g63980
ORF Names:MBM17.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G63980

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi287 – 353Missing in fry1-1; abolishes activity. Add BLAST67

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001425301 – 353SAL1 phosphataseAdd BLAST353

Proteomic databases

PaxDbiQ42546
PRIDEiQ42546

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques.2 Publications

Gene expression databases

ExpressionAtlasiQ42546 baseline and differential
GenevisibleiQ42546 AT

Interactioni

Protein-protein interaction databases

BioGridi21761, 6 interactors
STRINGi3702.AT5G63980.1

Structurei

Secondary structure

1353
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 22Combined sources19
Helixi44 – 60Combined sources17
Helixi83 – 89Combined sources7
Helixi110 – 118Combined sources9
Beta strandi128 – 135Combined sources8
Turni139 – 144Combined sources6
Beta strandi150 – 156Combined sources7
Beta strandi159 – 169Combined sources11
Beta strandi190 – 195Combined sources6
Turni196 – 198Combined sources3
Beta strandi199 – 203Combined sources5
Turni205 – 207Combined sources3
Beta strandi227 – 229Combined sources3
Beta strandi233 – 235Combined sources3
Beta strandi241 – 243Combined sources3
Helixi244 – 247Combined sources4
Helixi262 – 268Combined sources7
Beta strandi272 – 276Combined sources5
Turni287 – 289Combined sources3
Helixi290 – 297Combined sources8
Turni298 – 300Combined sources3
Beta strandi302 – 308Combined sources7
Beta strandi325 – 328Combined sources4
Beta strandi330 – 334Combined sources5
Helixi335 – 339Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ESYX-ray3.05A/B1-346[»]
ProteinModelPortaliQ42546
SMRiQ42546
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 139Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1528 Eukaryota
COG1218 LUCA
HOGENOMiHOG000170673
InParanoidiQ42546
KOiK15422
OrthoDBiEOG09360EFU
PhylomeDBiQ42546

Family and domain databases

InterProiView protein in InterPro
IPR006239 Bisphos_HAL2
IPR020583 Inositol_monoP_metal-BS
IPR000760 Inositol_monophosphatase-like
PfamiView protein in Pfam
PF00459 Inositol_P, 1 hit
PRINTSiPR00377 IMPHPHTASES
TIGRFAMsiTIGR01330 bisphos_HAL2, 1 hit
PROSITEiView protein in PROSITE
PS00629 IMP_1, 1 hit

Sequencei

Sequence statusi: Complete.

Q42546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYEKELDAA KKAASLAARL CQKVQKALLQ SDVQSKSDKS PVTVADYGSQ
60 70 80 90 100
AVVSLVLEKE LSSEPFSLVA EEDSGDLRKD GSQDTLERIT KLVNDTLATE
110 120 130 140 150
ESFNGSTLST DDLLRAIDCG TSEGGPNGRH WVLDPIDGTK GFLRGDQYAV
160 170 180 190 200
ALGLLEEGKV VLGVLACPNL PLASIAGNNK NKSSSDEIGC LFFATIGSGT
210 220 230 240 250
YMQLLDSKSS PVKVQVSSVE NPEEASFFES FEGAHSLHDL SSSIANKLGV
260 270 280 290 300
KAPPVRIDSQ AKYGALSRGD GAIYLRFPHK GYREKIWDHV AGAIVVTEAG
310 320 330 340 350
GIVTDAAGKP LDFSKGKYLD LDTGIIVANE KLMPLLLKAV RDSIAEQEKA

SAL
Length:353
Mass (Da):37,564
Last modified:November 1, 1996 - v1
Checksum:i351EBB6826B93A4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40433 mRNA Translation: AAC49263.1
AY034894 mRNA Translation: AAK58887.1
AB019227 Genomic DNA Translation: BAA96901.1
CP002688 Genomic DNA Translation: AED97825.2
BT005993 mRNA Translation: AAO64928.1
AK227460 mRNA Translation: BAE99463.1
RefSeqiNP_201203.3, NM_125794.5
UniGeneiAt.9555

Genome annotation databases

EnsemblPlantsiAT5G63980.1; AT5G63980.1; AT5G63980
GeneIDi836519
GrameneiAT5G63980.1; AT5G63980.1; AT5G63980
KEGGiath:AT5G63980

Similar proteinsi

Entry informationi

Entry nameiDPNP1_ARATH
AccessioniPrimary (citable) accession number: Q42546
Secondary accession number(s): F4KC73, Q0WTT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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