Q42538 (SAT5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine acetyltransferase 5
Short name=AtSAT-5
EC=2.3.1.30

Alternative name(s):
AtSERAT1;1
SAT-c

Gene names

Name:	SAT5
Synonyms:	SAT52
Ordered Locus Names:	At5g56760
ORF Names:	MIK19.23

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.
Enzyme regulation	Feedback inhibitions by L-Ser and acetyl-CoA. Ref.7
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 1/2.
Subunit structure	Homomultimer By similarity.
Subcellular location	Cytoplasm Ref.7.
Tissue specificity	Mostly expressed in stems, flowers and siliques. Localized in vascular tissues, particularly in phloem. Ref.8 Ref.9
Induction	By cadmium (Cd). Not induced under sulfur-deficient conditions. Ref.7 Ref.8 Ref.9
Sequence similarities	Belongs to the transferase hexapeptide repeat family.
Biophysicochemical properties	Kinetic parameters: KM=2.71 mM for L-Ser (at pH 8 and 37 degrees Celsius) Ref.7 KM=0.28 mM for acetyl-CoA (at pH 8 and 37 degrees Celsius)

Ontologies

Keywords
Biological process	Amino-acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay Ref.7. Source: TAIR
Molecular function	serine O-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Serine acetyltransferase 5		PRO_0000068693
Regions
	Compositional bias	26 – 64	39	Ala-rich
Experimental info
	Sequence conflict	23	1	S → F in AAM61424. Ref.5
	Sequence conflict	30	1	S → A in AAM61424. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q42538 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 15835510FF314A08	FASTA	312	32,770
	10 20 30 40 50 60 MPPAGELRHQ SPSKEKLSSV TQSDEAEAAS AAISAAAADA EAAGLWTQIK AEARRDAEAE 70 80 90 100 110 120 PALASYLYST ILSHSSLERS ISFHLGNKLC SSTLLSTLLY DLFLNTFSSD PSLRNATVAD 130 140 150 160 170 180 LRAARVRDPA CISFSHCLLN YKGFLAIQAH RVSHKLWTQS RKPLALALHS RISDVFAVDI 190 200 210 220 230 240 HPAAKIGKGI LLDHATGVVV GETAVIGNNV SILHHVTLGG TGKACGDRHP KIGDGCLIGA 250 260 270 280 290 300 GATILGNVKI GAGAKVGAGS VVLIDVPCRG TAVGNPARLV GGKEKPTIHD EECPGESMDH 310 TSFISEWSDY II

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cysteine biosynthesis in higher plants: a new member of the Arabidopsis thaliana serine acetyltransferase small gene-family obtained by functional complementation of an Escherichia coli cysteine auxotroph." Howarth J.R., Roberts M.A., Wray J.L. Biochim. Biophys. Acta 1350:123-127(1997) [PubMed: 9048879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Isoform-dependent differences in feedback regulation and subcellular localization of serine acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana." Noji M., Inoue K., Kimura N., Gouda A., Saito K. J. Biol. Chem. 273:32739-32745(1998) [PubMed: 9830017] [Abstract] Cited for: ENZYME REGULATION, SUBCELLULAR LOCATION, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"The serine acetyltransferase gene family in Arabidopsis thaliana and the regulation of its expression by cadmium." Howarth J.R., Dominguez-Solis J.R., Gutierrez-Alcala G., Wray J.L., Romero L.C., Gotor C. Plant Mol. Biol. 51:589-598(2003) [PubMed: 12650624] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION. Strain: cv. Columbia.
[9]	"Characterization and expression analysis of a serine acetyltransferase gene family involved in a key step of the sulfur assimilation pathway in Arabidopsis." Kawashima C.G., Berkowitz O., Hell R., Noji M., Saito K. Plant Physiol. 137:220-230(2005) [PubMed: 15579666] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30298 mRNA. Translation: AAC49655.1. AB013392 Genomic DNA. Translation: BAB09894.1. CP002688 Genomic DNA. Translation: AED96804.1. AY039612 mRNA. Translation: AAK62667.1. AY133674 mRNA. Translation: AAM91504.1. AK227979 mRNA. Translation: BAE99945.1. AY084861 mRNA. Translation: AAM61424.1.
IPI	IPI00517479.
PIR	S71207.
RefSeq	NP_200487.1. NM_125059.2.
UniGene	At.23802.
3D structure databases
ProteinModelPortal	Q42538.
SMR	Q42538. Positions 8-307.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42538. 1 interaction.
STRING	Q42538.
Proteomic databases
PRIDE	Q42538.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G56760.1; AT5G56760.1; AT5G56760.
GeneID	835778.
GenomeReviews	Gene locus AT5G56760 in contig BA000015_GR.
KEGG	ath:AT5G56760.
NMPDR	fig|3702.1.peg.27616.
Organism-specific databases
GeneFarm	5181. 495.
TAIR	At5g56760.
Phylogenomic databases
eggNOG	KOG4750.
GeneTree	EPGT00050000004084.
HOGENOM	HBG754554.
InParanoid	Q42538.
OMA	LALHSRI.
PhylomeDB	Q42538.
ProtClustDB	PLN02694.
Enzyme and pathway databases
BRENDA	2.3.1.30. 399.
Gene expression databases
Genevestigator	Q42538.
GermOnline	AT5G56760. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR010493. Ser_AcTrfase_N. IPR005881. Ser_O-AcTrfase. IPR011004. Trimer_LpxA-like. [Graphical view]
KO	K00640.
Pfam	PF00132. Hexapep. 1 hit. PF06426. SATase_N. 1 hit. [Graphical view]
SMART	SM00971. SATase_N. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01172. CysE. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAT5_ARATH

Accession

Primary (citable) accession number: Q42538
Secondary accession number(s): Q0WSF3, Q8LFG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2006
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program