ID   PORA_ARATH              Reviewed;         405 AA.
AC   Q42536; Q9FK22;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2002, sequence version 2.
DT   16-JUN-2009, entry version 77.
DE   RecName: Full=Protochlorophyllide reductase A, chloroplastic;
DE            Short=PCR A;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase A;
DE            Short=POR A;
DE   Flags: Precursor;
GN   Name=PORA; OrderedLocusNames=At5g54190; ORFNames=K18G13.7;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=95388766; PubMed=7659751; DOI=10.1104/pp.108.4.1505;
RA   Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.;
RT   "Identification of NADPH:protochlorophyllide oxidoreductases A and B:
RT   a branched pathway for light-dependent chlorophyll biosynthesis in
RT   Arabidopsis thaliana.";
RL   Plant Physiol. 108:1505-1517(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=98403884; PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA   Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VI.
RT   Sequence features of the regions of 1,367,185 bp covered by 19
RT   physically assigned P1 and TAC clones.";
RL   DNA Res. 5:203-216(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide). PORA may also function as a
CC       photoprotectant during the transitory stage from dark to light.
CC   -!- CATALYTIC ACTIVITY: Chlorophyllide a + NADP(+) =
CC       protochlorophyllide + NADPH.
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences;
CC       Name=1;
CC         IsoId=Q42536-1; Sequence=Displayed;
CC   -!- TISSUE SPECIFICITY: Expressed in young seedlings.
CC   -!- DEVELOPMENTAL STAGE: Etiolated seedlings.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. POR subfamily.
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DR   EMBL; U29699; AAC49043.1; -; mRNA.
DR   EMBL; AB013387; BAB11581.1; -; Genomic_DNA.
DR   EMBL; BT003853; AAO41903.1; -; mRNA.
DR   EMBL; BT005080; AAO50613.1; -; mRNA.
DR   IPI; IPI00545356; -.
DR   RefSeq; NP_200230.1; -.
DR   UniGene; At.23877; -.
DR   HSSP; P14061; 1FDU.
DR   PRIDE; Q42536; -.
DR   GeneID; 835507; -.
DR   GenomeReviews; BA000015_GR; AT5G54190.
DR   NMPDR; fig|3702.1.peg.27326; -.
DR   TAIR; At5g54190; -.
DR   OMA; Q42536; HRRFHED.
DR   BRENDA; 1.3.1.33; 302.
DR   ArrayExpress; Q42536; -.
DR   GO; GO:0009507; C:chloroplast; NAS:TAIR.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; NAS:TAIR.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR005979; Prochl_reduct.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   TIGRFAMs; TIGR01289; LPOR; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Chlorophyll biosynthesis; Chloroplast;
KW   Complete proteome; NADP; Oxidoreductase; Photosynthesis; Plastid;
KW   Transit peptide.
FT   TRANSIT       1     69       Chloroplast (Potential).
FT   CHAIN        70    405       Protochlorophyllide reductase A,
FT                                chloroplastic.
FT                                /FTId=PRO_0000023287.
FT   CONFLICT     37     37       V -> I (in Ref. 1; AAC49043).
SQ   SEQUENCE   405 AA;  43863 MW;  EB82F2CEF0480D2E CRC64;
     MALQAASLVS SAFSVRKDGK LNASASSSFK ESSLFGVSLS EQSKADFVSS SLRCKREQSL
     RNNKAIIRAQ AIATSTPSVT KSSLDRKKTL RKGNVVVTGA SSGLGLATAK ALAETGKWHV
     IMACRDFLKA ERAAQSAGMP KDSYTVMHLD LASLDSVRQF VDNFRRAEMP LDVLVCNAAV
     YQPTANQPTF TAEGFELSVG INHLGHFLLS RLLIDDLKNS DYPSKRLIIV GSITGNTNTL
     AGNVPPKANL GDLRGLAGGL NGLNSSAMID GGDFVGAKAY KDSKVCNMLT MQEFHRRFHE
     DTGITFASLY PGCIATTGLF REHIPLFRTL FPPFQKYITK GYVSESEAGK RLAQVVADPS
     LTKSGVYWSW NKTSASFENQ LSQEASDVEK ARRVWEVSEK LVGLA
//