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Q42536 (PORA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protochlorophyllide reductase A, chloroplastic

Short name=PCR A
EC=1.3.1.33
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name=POR A
Gene names
Name:PORA
Ordered Locus Names:At5g54190
ORF Names:K18G13.7
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length405 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). PORA may also function as a photoprotectant during the transitory stage from dark to light. Functions in skotomorphogenesis, photomorphogenesis and throughout the plant life under specific light conditions. Ref.6 Ref.9 Ref.10

Catalytic activity

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathway

Porphyrin-containing compound metabolism; chlorophyll biosynthesis.

Subunit structure

Forms large complexes including TOC33, pPORA and OEP161 during pPORA import into plastids at the plastid envelope membrane.

Subcellular location

Plastidchloroplast Ref.8.

Tissue specificity

Expressed in young seedlings. Not detected in leaves. Ref.1

Developmental stage

Etiolated seedlings. Ref.1 Ref.6

Induction

Down-regulated by light. Ref.6

Disruption phenotype

Lethal under normal growth conditions and light-green stunted plants when grown in presence of sucrose. Ref.10

Miscellaneous

The presence of TOC33 is not required for the import of PORA into plastids (Ref.8).

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q42536-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q42536-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 6969Chloroplast Potential
Chain70 – 405336Protochlorophyllide reductase A, chloroplastic
PRO_0000023287

Natural variations

Alternative sequence1 – 121121Missing in isoform 2.
VSP_046548

Experimental info

Sequence conflict371V → I in AAC49043. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 6, 2002. Version 2.
Checksum: EB82F2CEF0480D2E

FASTA40543,863
        10         20         30         40         50         60 
MALQAASLVS SAFSVRKDGK LNASASSSFK ESSLFGVSLS EQSKADFVSS SLRCKREQSL 

        70         80         90        100        110        120 
RNNKAIIRAQ AIATSTPSVT KSSLDRKKTL RKGNVVVTGA SSGLGLATAK ALAETGKWHV 

       130        140        150        160        170        180 
IMACRDFLKA ERAAQSAGMP KDSYTVMHLD LASLDSVRQF VDNFRRAEMP LDVLVCNAAV 

       190        200        210        220        230        240 
YQPTANQPTF TAEGFELSVG INHLGHFLLS RLLIDDLKNS DYPSKRLIIV GSITGNTNTL 

       250        260        270        280        290        300 
AGNVPPKANL GDLRGLAGGL NGLNSSAMID GGDFVGAKAY KDSKVCNMLT MQEFHRRFHE 

       310        320        330        340        350        360 
DTGITFASLY PGCIATTGLF REHIPLFRTL FPPFQKYITK GYVSESEAGK RLAQVVADPS 

       370        380        390        400 
LTKSGVYWSW NKTSASFENQ LSQEASDVEK ARRVWEVSEK LVGLA 

« Hide

Isoform 2 [UniParc].

Checksum: 32B0E96EB4EF2DFF
Show »

FASTA28431,208

References

« Hide 'large scale' references
[1]"Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[6]"POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
Su Q., Frick G., Armstrong G., Apel K.
Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION BY LIGHT, DEVELOPMENTAL STAGE.
Strain: cv. Columbia.
[7]"A role of Toc33 in the protochlorophyllide-dependent plastid import pathway of NADPH:protochlorophyllide oxidoreductase (POR) A."
Reinbothe S., Pollmann S., Springer A., James R.J., Tichtinsky G., Reinbothe C.
Plant J. 42:1-12(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH OP161 AND TOC33.
[8]"Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings."
Kim C., Ham H., Apel K.
Plant J. 42:329-340(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[9]"Arabidopsis protochlorophyllide oxidoreductase A (PORA) restores bulk chlorophyll synthesis and normal development to a porB porC double mutant."
Paddock T.N., Mason M.E., Lima D.F., Armstrong G.A.
Plant Mol. Biol. 72:445-457(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Arabidopsis light-dependent protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development."
Paddock T., Lima D., Mason M.E., Apel K., Armstrong G.A.
Plant Mol. Biol. 78:447-460(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U29699 mRNA. Translation: AAC49043.1.
AB013387 Genomic DNA. Translation: BAB11581.1.
CP002688 Genomic DNA. Translation: AED96464.1.
CP002688 Genomic DNA. Translation: AED96465.1.
BT003853 mRNA. Translation: AAO41903.1.
BT005080 mRNA. Translation: AAO50613.1.
AK317329 mRNA. Translation: BAH20003.1.
RefSeqNP_001032072.1. NM_001036995.1. [Q42536-2]
NP_200230.1. NM_124799.3. [Q42536-1]
UniGeneAt.23877.

3D structure databases

ProteinModelPortalQ42536.
SMRQ42536. Positions 95-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20751. 4 interactions.
IntActQ42536. 1 interaction.
STRING3702.AT5G54190.1-P.

Proteomic databases

PRIDEQ42536.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G54190.1; AT5G54190.1; AT5G54190. [Q42536-1]
GeneID835507.
KEGGath:AT5G54190.

Organism-specific databases

TAIRAT5G54190.

Phylogenomic databases

eggNOGCOG1028.
InParanoidQ42536.
KOK00218.
OMAFLLCNLM.
PhylomeDBQ42536.

Enzyme and pathway databases

BioCycARA:AT5G54190-MONOMER.
ARA:GQT-971-MONOMER.
BRENDA1.3.1.33. 399.
UniPathwayUPA00668.

Gene expression databases

GenevestigatorQ42536.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
TIGRFAMsTIGR01289. LPOR. 1 hit.
ProtoNetSearch...

Entry information

Entry namePORA_ARATH
AccessionPrimary (citable) accession number: Q42536
Secondary accession number(s): B9DGY6, Q9FK22
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 6, 2002
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names