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Protein

Protochlorophyllide reductase A, chloroplastic

Gene

PORA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). PORA may also function as a photoprotectant during the transitory stage from dark to light. Functions in skotomorphogenesis, photomorphogenesis and throughout the plant life under specific light conditions.3 Publications

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • protochlorophyllide reductase activity Source: TAIR

GO - Biological processi

  • chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  • photosynthesis Source: UniProtKB-KW
  • response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT5G54190-MONOMER.
BRENDAi1.3.1.33. 399.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase A, chloroplastic (EC:1.3.1.33)
Short name:
PCR A
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name:
POR A
Gene namesi
Name:PORA
Ordered Locus Names:At5g54190
ORF Names:K18G13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G54190.

Subcellular locationi

  • Plastidchloroplast 1 Publication

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal under normal growth conditions and light-green stunted plants when grown in presence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 69ChloroplastSequence analysisAdd BLAST69
ChainiPRO_000002328770 – 405Protochlorophyllide reductase A, chloroplasticAdd BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70Pyrrolidone carboxylic acidBy similarity1

Keywords - PTMi

Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiQ42536.
PRIDEiQ42536.

Expressioni

Tissue specificityi

Expressed in young seedlings. Not detected in leaves.1 Publication

Developmental stagei

Etiolated seedlings.2 Publications

Inductioni

Down-regulated by light.1 Publication

Gene expression databases

GenevisibleiQ42536. AT.

Interactioni

Subunit structurei

Forms large complexes including TOC33, pPORA and OEP161 during pPORA import into plastids at the plastid envelope membrane. Interacts with CPP1 during plastid import (PubMed:25901327).1 Publication

Protein-protein interaction databases

BioGridi20751. 6 interactors.
IntActiQ42536. 1 interactor.
STRINGi3702.AT5G54190.1.

Structurei

3D structure databases

ProteinModelPortaliQ42536.
SMRiQ42536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1208. Eukaryota.
COG1028. LUCA.
InParanoidiQ42536.
KOiK00218.
OMAiSDHVKAD.
OrthoDBiEOG09360CR9.
PhylomeDBiQ42536.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q42536-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALQAASLVS SAFSVRKDGK LNASASSSFK ESSLFGVSLS EQSKADFVSS
60 70 80 90 100
SLRCKREQSL RNNKAIIRAQ AIATSTPSVT KSSLDRKKTL RKGNVVVTGA
110 120 130 140 150
SSGLGLATAK ALAETGKWHV IMACRDFLKA ERAAQSAGMP KDSYTVMHLD
160 170 180 190 200
LASLDSVRQF VDNFRRAEMP LDVLVCNAAV YQPTANQPTF TAEGFELSVG
210 220 230 240 250
INHLGHFLLS RLLIDDLKNS DYPSKRLIIV GSITGNTNTL AGNVPPKANL
260 270 280 290 300
GDLRGLAGGL NGLNSSAMID GGDFVGAKAY KDSKVCNMLT MQEFHRRFHE
310 320 330 340 350
DTGITFASLY PGCIATTGLF REHIPLFRTL FPPFQKYITK GYVSESEAGK
360 370 380 390 400
RLAQVVADPS LTKSGVYWSW NKTSASFENQ LSQEASDVEK ARRVWEVSEK

LVGLA
Length:405
Mass (Da):43,863
Last modified:December 6, 2002 - v2
Checksum:iEB82F2CEF0480D2E
GO
Isoform 2 (identifier: Q42536-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Show »
Length:284
Mass (Da):31,208
Checksum:i32B0E96EB4EF2DFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti37V → I in AAC49043 (PubMed:7659751).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0465481 – 121Missing in isoform 2. 1 PublicationAdd BLAST121

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29699 mRNA. Translation: AAC49043.1.
AB013387 Genomic DNA. Translation: BAB11581.1.
CP002688 Genomic DNA. Translation: AED96464.1.
CP002688 Genomic DNA. Translation: AED96465.1.
BT003853 mRNA. Translation: AAO41903.1.
BT005080 mRNA. Translation: AAO50613.1.
AK317329 mRNA. Translation: BAH20003.1.
RefSeqiNP_001032072.1. NM_001036995.1. [Q42536-2]
NP_200230.1. NM_124799.4. [Q42536-1]
UniGeneiAt.23877.

Genome annotation databases

EnsemblPlantsiAT5G54190.1; AT5G54190.1; AT5G54190. [Q42536-1]
GeneIDi835507.
GrameneiAT5G54190.1; AT5G54190.1; AT5G54190.
KEGGiath:AT5G54190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29699 mRNA. Translation: AAC49043.1.
AB013387 Genomic DNA. Translation: BAB11581.1.
CP002688 Genomic DNA. Translation: AED96464.1.
CP002688 Genomic DNA. Translation: AED96465.1.
BT003853 mRNA. Translation: AAO41903.1.
BT005080 mRNA. Translation: AAO50613.1.
AK317329 mRNA. Translation: BAH20003.1.
RefSeqiNP_001032072.1. NM_001036995.1. [Q42536-2]
NP_200230.1. NM_124799.4. [Q42536-1]
UniGeneiAt.23877.

3D structure databases

ProteinModelPortaliQ42536.
SMRiQ42536.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi20751. 6 interactors.
IntActiQ42536. 1 interactor.
STRINGi3702.AT5G54190.1.

Proteomic databases

PaxDbiQ42536.
PRIDEiQ42536.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G54190.1; AT5G54190.1; AT5G54190. [Q42536-1]
GeneIDi835507.
GrameneiAT5G54190.1; AT5G54190.1; AT5G54190.
KEGGiath:AT5G54190.

Organism-specific databases

TAIRiAT5G54190.

Phylogenomic databases

eggNOGiKOG1208. Eukaryota.
COG1028. LUCA.
InParanoidiQ42536.
KOiK00218.
OMAiSDHVKAD.
OrthoDBiEOG09360CR9.
PhylomeDBiQ42536.

Enzyme and pathway databases

UniPathwayiUPA00668.
BioCyciARA:AT5G54190-MONOMER.
BRENDAi1.3.1.33. 399.

Miscellaneous databases

PROiQ42536.

Gene expression databases

GenevisibleiQ42536. AT.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
SUPFAMiSSF51735. SSF51735. 2 hits.
TIGRFAMsiTIGR01289. LPOR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPORA_ARATH
AccessioniPrimary (citable) accession number: Q42536
Secondary accession number(s): B9DGY6, Q9FK22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 6, 2002
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The presence of TOC33 is not required for the import of PORA into plastids.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.