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Q42536

- PORA_ARATH

UniProt

Q42536 - PORA_ARATH

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Protein

Protochlorophyllide reductase A, chloroplastic

Gene

PORA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). PORA may also function as a photoprotectant during the transitory stage from dark to light. Functions in skotomorphogenesis, photomorphogenesis and throughout the plant life under specific light conditions.3 Publications

Catalytic activityi

Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.

Pathwayi

GO - Molecular functioni

  1. protochlorophyllide reductase activity Source: TAIR

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-UniPathway
  2. photosynthesis Source: UniProtKB-KW
  3. response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Chlorophyll biosynthesis, Photosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciARA:AT5G54190-MONOMER.
ARA:GQT-971-MONOMER.
BRENDAi1.3.1.33. 399.
UniPathwayiUPA00668.

Names & Taxonomyi

Protein namesi
Recommended name:
Protochlorophyllide reductase A, chloroplastic (EC:1.3.1.33)
Short name:
PCR A
Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name:
POR A
Gene namesi
Name:PORA
Ordered Locus Names:At5g54190
ORF Names:K18G13.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G54190.

Subcellular locationi

Plastidchloroplast 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast envelope Source: TAIR
  3. chloroplast thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Lethal under normal growth conditions and light-green stunted plants when grown in presence of sucrose.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6969ChloroplastSequence AnalysisAdd
BLAST
Chaini70 – 405336Protochlorophyllide reductase A, chloroplasticPRO_0000023287Add
BLAST

Proteomic databases

PRIDEiQ42536.

Expressioni

Tissue specificityi

Expressed in young seedlings. Not detected in leaves.1 Publication

Developmental stagei

Etiolated seedlings.2 Publications

Inductioni

Down-regulated by light.1 Publication

Gene expression databases

GenevestigatoriQ42536.

Interactioni

Subunit structurei

Forms large complexes including TOC33, pPORA and OEP161 during pPORA import into plastids at the plastid envelope membrane.

Protein-protein interaction databases

BioGridi20751. 4 interactions.
IntActiQ42536. 1 interaction.
STRINGi3702.AT5G54190.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ42536.
SMRiQ42536. Positions 95-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1028.
InParanoidiQ42536.
KOiK00218.
OMAiFLLCNLM.
PhylomeDBiQ42536.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
TIGRFAMsiTIGR01289. LPOR. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q42536-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALQAASLVS SAFSVRKDGK LNASASSSFK ESSLFGVSLS EQSKADFVSS
60 70 80 90 100
SLRCKREQSL RNNKAIIRAQ AIATSTPSVT KSSLDRKKTL RKGNVVVTGA
110 120 130 140 150
SSGLGLATAK ALAETGKWHV IMACRDFLKA ERAAQSAGMP KDSYTVMHLD
160 170 180 190 200
LASLDSVRQF VDNFRRAEMP LDVLVCNAAV YQPTANQPTF TAEGFELSVG
210 220 230 240 250
INHLGHFLLS RLLIDDLKNS DYPSKRLIIV GSITGNTNTL AGNVPPKANL
260 270 280 290 300
GDLRGLAGGL NGLNSSAMID GGDFVGAKAY KDSKVCNMLT MQEFHRRFHE
310 320 330 340 350
DTGITFASLY PGCIATTGLF REHIPLFRTL FPPFQKYITK GYVSESEAGK
360 370 380 390 400
RLAQVVADPS LTKSGVYWSW NKTSASFENQ LSQEASDVEK ARRVWEVSEK

LVGLA
Length:405
Mass (Da):43,863
Last modified:December 6, 2002 - v2
Checksum:iEB82F2CEF0480D2E
GO
Isoform 2 (identifier: Q42536-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-121: Missing.

Show »
Length:284
Mass (Da):31,208
Checksum:i32B0E96EB4EF2DFF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371V → I in AAC49043. (PubMed:7659751)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 121121Missing in isoform 2. 1 PublicationVSP_046548Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29699 mRNA. Translation: AAC49043.1.
AB013387 Genomic DNA. Translation: BAB11581.1.
CP002688 Genomic DNA. Translation: AED96464.1.
CP002688 Genomic DNA. Translation: AED96465.1.
BT003853 mRNA. Translation: AAO41903.1.
BT005080 mRNA. Translation: AAO50613.1.
AK317329 mRNA. Translation: BAH20003.1.
RefSeqiNP_001032072.1. NM_001036995.1. [Q42536-2]
NP_200230.1. NM_124799.3. [Q42536-1]
UniGeneiAt.23877.

Genome annotation databases

EnsemblPlantsiAT5G54190.1; AT5G54190.1; AT5G54190. [Q42536-1]
GeneIDi835507.
KEGGiath:AT5G54190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U29699 mRNA. Translation: AAC49043.1 .
AB013387 Genomic DNA. Translation: BAB11581.1 .
CP002688 Genomic DNA. Translation: AED96464.1 .
CP002688 Genomic DNA. Translation: AED96465.1 .
BT003853 mRNA. Translation: AAO41903.1 .
BT005080 mRNA. Translation: AAO50613.1 .
AK317329 mRNA. Translation: BAH20003.1 .
RefSeqi NP_001032072.1. NM_001036995.1. [Q42536-2 ]
NP_200230.1. NM_124799.3. [Q42536-1 ]
UniGenei At.23877.

3D structure databases

ProteinModelPortali Q42536.
SMRi Q42536. Positions 95-298.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20751. 4 interactions.
IntActi Q42536. 1 interaction.
STRINGi 3702.AT5G54190.1-P.

Proteomic databases

PRIDEi Q42536.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G54190.1 ; AT5G54190.1 ; AT5G54190 . [Q42536-1 ]
GeneIDi 835507.
KEGGi ath:AT5G54190.

Organism-specific databases

TAIRi AT5G54190.

Phylogenomic databases

eggNOGi COG1028.
InParanoidi Q42536.
KOi K00218.
OMAi FLLCNLM.
PhylomeDBi Q42536.

Enzyme and pathway databases

UniPathwayi UPA00668 .
BioCyci ARA:AT5G54190-MONOMER.
ARA:GQT-971-MONOMER.
BRENDAi 1.3.1.33. 399.

Gene expression databases

Genevestigatori Q42536.

Family and domain databases

Gene3Di 3.40.50.720. 2 hits.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR005979. Prochl_reduct.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
TIGRFAMsi TIGR01289. LPOR. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana."
    Armstrong G.A., Runge S., Frick G., Sperling U., Apel K.
    Plant Physiol. 108:1505-1517(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones."
    Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S.
    DNA Res. 5:203-216(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
  6. "POR C of Arabidopsis thaliana: a third light- and NADPH-dependent protochlorophyllide oxidoreductase that is differentially regulated by light."
    Su Q., Frick G., Armstrong G., Apel K.
    Plant Mol. Biol. 47:805-813(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY LIGHT, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  7. "A role of Toc33 in the protochlorophyllide-dependent plastid import pathway of NADPH:protochlorophyllide oxidoreductase (POR) A."
    Reinbothe S., Pollmann S., Springer A., James R.J., Tichtinsky G., Reinbothe C.
    Plant J. 42:1-12(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH OP161 AND TOC33.
  8. "Multiplicity of different cell- and organ-specific import routes for the NADPH-protochlorophyllide oxidoreductases A and B in plastids of Arabidopsis seedlings."
    Kim C., Ham H., Apel K.
    Plant J. 42:329-340(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  9. "Arabidopsis protochlorophyllide oxidoreductase A (PORA) restores bulk chlorophyll synthesis and normal development to a porB porC double mutant."
    Paddock T.N., Mason M.E., Lima D.F., Armstrong G.A.
    Plant Mol. Biol. 72:445-457(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Arabidopsis light-dependent protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development."
    Paddock T., Lima D., Mason M.E., Apel K., Armstrong G.A.
    Plant Mol. Biol. 78:447-460(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiPORA_ARATH
AccessioniPrimary (citable) accession number: Q42536
Secondary accession number(s): B9DGY6, Q9FK22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 6, 2002
Last modified: October 1, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The presence of TOC33 is not required for the import of PORA into plastids.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3