Q42536 (PORA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protochlorophyllide reductase A, chloroplastic
Short name=PCR A
EC=1.3.1.33

Alternative name(s):
NADPH-protochlorophyllide oxidoreductase A
Short name=POR A

Gene names

Name:	PORA
Ordered Locus Names:	At5g54190
ORF Names:	K18G13.7

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide). PORA may also function as a photoprotectant during the transitory stage from dark to light.
Catalytic activity	Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Expressed in young seedlings.
Developmental stage	Etiolated seedlings.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW response to ethylene stimulus Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast envelope Inferred from direct assay. Source: TAIR chloroplast thylakoid Inferred from direct assay. Source: TAIR
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Non-traceable author statement. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42536-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 69	69	Chloroplast Potential
	Chain	70 – 405	336	Protochlorophyllide reductase A, chloroplastic		PRO_0000023287
Experimental info
	Sequence conflict	37	1	V → I in AAC49043. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: EB82F2CEF0480D2E	FASTA	405	43,863
	10 20 30 40 50 60 MALQAASLVS SAFSVRKDGK LNASASSSFK ESSLFGVSLS EQSKADFVSS SLRCKREQSL 70 80 90 100 110 120 RNNKAIIRAQ AIATSTPSVT KSSLDRKKTL RKGNVVVTGA SSGLGLATAK ALAETGKWHV 130 140 150 160 170 180 IMACRDFLKA ERAAQSAGMP KDSYTVMHLD LASLDSVRQF VDNFRRAEMP LDVLVCNAAV 190 200 210 220 230 240 YQPTANQPTF TAEGFELSVG INHLGHFLLS RLLIDDLKNS DYPSKRLIIV GSITGNTNTL 250 260 270 280 290 300 AGNVPPKANL GDLRGLAGGL NGLNSSAMID GGDFVGAKAY KDSKVCNMLT MQEFHRRFHE 310 320 330 340 350 360 DTGITFASLY PGCIATTGLF REHIPLFRTL FPPFQKYITK GYVSESEAGK RLAQVVADPS 370 380 390 400 LTKSGVYWSW NKTSASFENQ LSQEASDVEK ARRVWEVSEK LVGLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of NADPH:protochlorophyllide oxidoreductases A and B: a branched pathway for light-dependent chlorophyll biosynthesis in Arabidopsis thaliana." Armstrong G.A., Runge S., Frick G., Sperling U., Apel K. Plant Physiol. 108:1505-1517(1995) [PubMed: 7659751] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U29699 mRNA. Translation: AAC49043.1. AB013387 Genomic DNA. Translation: BAB11581.1. CP002688 Genomic DNA. Translation: AED96464.1. BT003853 mRNA. Translation: AAO41903.1. BT005080 mRNA. Translation: AAO50613.1.
IPI	IPI00545356.
RefSeq	NP_200230.1. NM_124799.3.
UniGene	At.23877.
3D structure databases
ProteinModelPortal	Q42536.
SMR	Q42536. Positions 82-405.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42536. 1 interaction.
STRING	Q42536.
Proteomic databases
PRIDE	Q42536.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G54190.1; AT5G54190.1; AT5G54190.
GeneID	835507.
GenomeReviews	Gene locus AT5G54190 in contig BA000015_GR.
KEGG	ath:AT5G54190.
NMPDR	fig|3702.1.peg.27326.
Organism-specific databases
TAIR	At5g54190.
Phylogenomic databases
GeneTree	EPGT00070000029180.
HOGENOM	HBG750976.
InParanoid	Q42536.
OMA	SWGNRQK.
PhylomeDB	Q42536.
ProtClustDB	PLN00015.
Enzyme and pathway databases
BRENDA	1.3.1.33. 399.
Gene expression databases
ArrayExpress	Q42536.
Genevestigator	Q42536.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR005979. Prochl_reduct. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00218.
PANTHER	PTHR24316:SF100. PTHR24316:SF100. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORA_ARATH

Accession

Primary (citable) accession number: Q42536
Secondary accession number(s): Q9FK22

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 6, 2002
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program