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Protein

Tryptophan synthase alpha chain, chloroplastic

Gene

TSA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Required for tryptophan biosynthesis. Contributes to the tryptophan-independent indole biosynthesis, and possibly to auxin production.4 Publications

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase, Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei96Proton acceptorBy similarity1
Active sitei107Proton acceptorBy similarity1

GO - Molecular functioni

  • indole-3-glycerol-phosphate lyase activity Source: UniProtKB-EC
  • tryptophan synthase activity Source: UniProtKB

GO - Biological processi

  • auxin biosynthetic process Source: UniProtKB-KW
  • defense response by callose deposition in cell wall Source: TAIR
  • defense response to bacterium Source: TAIR
  • gravitropism Source: UniProtKB
  • response to cytokinin Source: TAIR
  • tryptophan biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Auxin biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G54640-MONOMER
UniPathwayiUPA00035; UER00044

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chain, chloroplastic (EC:4.2.1.20)
Alternative name(s):
Indole-3-glycerol-phosphate lyase, chloroplastic (EC:4.1.2.8)
Protein TRYPTOPHAN-REQUIRING 3
Gene namesi
Name:TSA1
Synonyms:TRP3, TSA2
Ordered Locus Names:At3g54640
ORF Names:T14E10.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G54640
TAIRilocus:3685290 AT3G54640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Compressed root wave phenotype on tilted agar surfaces. Reduced accumulation of tryptophan, but increased levels of auxin.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 40Chloroplast1 PublicationAdd BLAST40
ChainiPRO_000042060541 – 312Tryptophan synthase alpha chain, chloroplasticAdd BLAST272

Proteomic databases

PaxDbiQ42529
PRIDEiQ42529

PTM databases

iPTMnetiQ42529

Expressioni

Tissue specificityi

Mostly expressed in roots, hypocotyls and leaves, and, to a lower extent, in seedlings, cotyledons, stems, inflorescences, flowers, siliques and seeds.1 Publication

Gene expression databases

ExpressionAtlasiQ42529 baseline and differential
GenevisibleiQ42529 AT

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G54640.1

Structurei

3D structure databases

ProteinModelPortaliQ42529
SMRiQ42529
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4175 Eukaryota
COG0159 LUCA
HOGENOMiHOG000223816
InParanoidiQ42529
KOiK01695
OMAiDYPPEEC
OrthoDBiEOG09360IXC
PhylomeDBiQ42529

Family and domain databases

CDDicd04724 Tryptophan_synthase_alpha, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00131 Trp_synth_alpha, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR011060 RibuloseP-bd_barrel
IPR018204 Trp_synthase_alpha_AS
IPR002028 Trp_synthase_suA
PfamiView protein in Pfam
PF00290 Trp_syntA, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit
TIGRFAMsiTIGR00262 trpA, 1 hit
PROSITEiView protein in PROSITE
PS00167 TRP_SYNTHASE_ALPHA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIAFKSGVF FLQSPKSQIG FRHSSPPDSS LSFKRFTPMA SLSTSSPTLG
60 70 80 90 100
LADTFTQLKK QGKVAFIPYI TAGDPDLSTT AEALKVLDAC GSDIIELGVP
110 120 130 140 150
YSDPLADGPV IQAAATRSLE RGTNLDSILE MLDKVVPQIS CPISLFTYYN
160 170 180 190 200
PILKRGLGKF MSSIRAVGVQ GLVVPDVPLE ETEMLRKEAL NNDIELVLLT
210 220 230 240 250
TPTTPTERMK LIVDASEGFI YLVSSIGVTG ARSSVSGKVQ SLLKDIKEAT
260 270 280 290 300
DKPVAVGFGI SKPEHVKQIA GWGADGVIVG SAMVKLLGDA KSPTEGLKEL
310
EKLTKSLKSA LL
Length:312
Mass (Da):33,200
Last modified:November 1, 1996 - v1
Checksum:i0B104A30219A54BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66F → C in BAE98558 (Ref. 5) Curated1
Sequence conflicti211L → R in AAM65526 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18993 Genomic DNA Translation: AAC49117.1
AL138656 Genomic DNA Translation: CAB77584.1
CP002686 Genomic DNA Translation: AEE79260.1
BT025875 mRNA Translation: ABF85777.1
AK226413 mRNA Translation: BAE98558.1
AY087980 mRNA Translation: AAM65526.1
PIRiS59519
RefSeqiNP_567004.1, NM_115321.4
UniGeneiAt.24508

Genome annotation databases

EnsemblPlantsiAT3G54640.1; AT3G54640.1; AT3G54640
GeneIDi824629
GrameneiAT3G54640.1; AT3G54640.1; AT3G54640
KEGGiath:AT3G54640

Similar proteinsi

Entry informationi

Entry nameiTRPA2_ARATH
AccessioniPrimary (citable) accession number: Q42529
Secondary accession number(s): Q0WWE0, Q8LA83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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