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Protein

Tryptophan synthase alpha chain, chloroplastic

Gene

TSA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. Required for tryptophan biosynthesis. Contributes to the tryptophan-independent indole biosynthesis, and possibly to auxin production.4 Publications

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase beta chain 2, chloroplastic (TSB2)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei96 – 961Proton acceptorBy similarity
Active sitei107 – 1071Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

  • auxin biosynthetic process Source: UniProtKB-KW
  • defense response by callose deposition in cell wall Source: TAIR
  • defense response to bacterium Source: TAIR
  • gravitropism Source: UniProtKB
  • response to cytokinin Source: TAIR
  • tryptophan biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Auxin biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G54640-MONOMER.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chain, chloroplastic (EC:4.2.1.20)
Alternative name(s):
Indole-3-glycerol-phosphate lyase, chloroplastic (EC:4.1.2.8)
Protein TRYPTOPHAN-REQUIRING 3
Gene namesi
Name:TSA1
Synonyms:TRP3, TSA2
Ordered Locus Names:At3g54640
ORF Names:T14E10.210
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G54640.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: UniProtKB
  • chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Compressed root wave phenotype on tilted agar surfaces. Reduced accumulation of tryptophan, but increased levels of auxin.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4040Chloroplast1 PublicationAdd
BLAST
Chaini41 – 312272Tryptophan synthase alpha chain, chloroplasticPRO_0000420605Add
BLAST

Proteomic databases

PaxDbiQ42529.
PRIDEiQ42529.

PTM databases

iPTMnetiQ42529.

Expressioni

Tissue specificityi

Mostly expressed in roots, hypocotyls and leaves, and, to a lower extent, in seedlings, cotyledons, stems, inflorescences, flowers, siliques and seeds.1 Publication

Gene expression databases

GenevisibleiQ42529. AT.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi3702.AT3G54640.1.

Structurei

3D structure databases

ProteinModelPortaliQ42529.
SMRiQ42529. Positions 51-311.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4175. Eukaryota.
COG0159. LUCA.
HOGENOMiHOG000223816.
InParanoidiQ42529.
KOiK01695.
OMAiACGSDII.
OrthoDBiEOG09360IXC.
PhylomeDBiQ42529.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIAFKSGVF FLQSPKSQIG FRHSSPPDSS LSFKRFTPMA SLSTSSPTLG
60 70 80 90 100
LADTFTQLKK QGKVAFIPYI TAGDPDLSTT AEALKVLDAC GSDIIELGVP
110 120 130 140 150
YSDPLADGPV IQAAATRSLE RGTNLDSILE MLDKVVPQIS CPISLFTYYN
160 170 180 190 200
PILKRGLGKF MSSIRAVGVQ GLVVPDVPLE ETEMLRKEAL NNDIELVLLT
210 220 230 240 250
TPTTPTERMK LIVDASEGFI YLVSSIGVTG ARSSVSGKVQ SLLKDIKEAT
260 270 280 290 300
DKPVAVGFGI SKPEHVKQIA GWGADGVIVG SAMVKLLGDA KSPTEGLKEL
310
EKLTKSLKSA LL
Length:312
Mass (Da):33,200
Last modified:November 1, 1996 - v1
Checksum:i0B104A30219A54BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661F → C in BAE98558 (Ref. 5) Curated
Sequence conflicti211 – 2111L → R in AAM65526 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18993 Genomic DNA. Translation: AAC49117.1.
AL138656 Genomic DNA. Translation: CAB77584.1.
CP002686 Genomic DNA. Translation: AEE79260.1.
BT025875 mRNA. Translation: ABF85777.1.
AK226413 mRNA. Translation: BAE98558.1.
AY087980 mRNA. Translation: AAM65526.1.
PIRiS59519.
RefSeqiNP_567004.1. NM_115321.3.
UniGeneiAt.24508.

Genome annotation databases

EnsemblPlantsiAT3G54640.1; AT3G54640.1; AT3G54640.
GeneIDi824629.
GrameneiAT3G54640.1; AT3G54640.1; AT3G54640.
KEGGiath:AT3G54640.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18993 Genomic DNA. Translation: AAC49117.1.
AL138656 Genomic DNA. Translation: CAB77584.1.
CP002686 Genomic DNA. Translation: AEE79260.1.
BT025875 mRNA. Translation: ABF85777.1.
AK226413 mRNA. Translation: BAE98558.1.
AY087980 mRNA. Translation: AAM65526.1.
PIRiS59519.
RefSeqiNP_567004.1. NM_115321.3.
UniGeneiAt.24508.

3D structure databases

ProteinModelPortaliQ42529.
SMRiQ42529. Positions 51-311.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G54640.1.

PTM databases

iPTMnetiQ42529.

Proteomic databases

PaxDbiQ42529.
PRIDEiQ42529.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G54640.1; AT3G54640.1; AT3G54640.
GeneIDi824629.
GrameneiAT3G54640.1; AT3G54640.1; AT3G54640.
KEGGiath:AT3G54640.

Organism-specific databases

TAIRiAT3G54640.

Phylogenomic databases

eggNOGiKOG4175. Eukaryota.
COG0159. LUCA.
HOGENOMiHOG000223816.
InParanoidiQ42529.
KOiK01695.
OMAiACGSDII.
OrthoDBiEOG09360IXC.
PhylomeDBiQ42529.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciARA:AT3G54640-MONOMER.

Miscellaneous databases

PROiQ42529.

Gene expression databases

GenevisibleiQ42529. AT.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA2_ARATH
AccessioniPrimary (citable) accession number: Q42529
Secondary accession number(s): Q0WWE0, Q8LA83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.