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Q42523 (MCCA_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
Short name=MCCase subunit alpha
EC=6.4.1.4
Alternative name(s):
3-methylcrotonyl-CoA carboxylase 1
3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha
Gene names
Name:MCCA
Ordered Locus Names:At1g03090
ORF Names:F10O3.9, F10O3_8
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 3-methylcrotonoyl-CoA + HCO3- = ADP + phosphate + 3-methylglutaconyl-CoA.

Cofactor

Biotin.

Binds 2 manganese ions per subunit By similarity.

Pathway

Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.

Subunit structure

Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits By similarity.

Subcellular location

Mitochondrion matrix.

Tissue specificity

In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos. Ref.5

Miscellaneous

Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.

Sequence similarities

Contains 1 ATP-grasp domain.

Contains 1 biotin carboxylation domain.

Contains 1 biotinyl-binding domain.

Sequence caution

The sequence AAD25800.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q42523-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q42523-2)

The sequence of this isoform differs from the canonical sequence as follows:
     281-300: Missing.
Note: May be due to exon skipping. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2525Mitochondrion Potential
Chain26 – 734709Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial
PRO_0000002835

Regions

Domain37 – 484448Biotin carboxylation
Domain156 – 354199ATP-grasp
Domain665 – 73268Biotinyl-binding

Sites

Active site3291 By similarity
Metal binding3111Manganese 1 By similarity
Metal binding3251Manganese 1 By similarity
Metal binding3251Manganese 2 By similarity
Metal binding3271Manganese 2 By similarity
Binding site1521ATP By similarity
Binding site2361ATP By similarity
Binding site2711ATP By similarity

Amino acid modifications

Modified residue6451Phosphoserine Ref.6
Modified residue6991N6-biotinyllysine By similarity

Natural variations

Alternative sequence281 – 30020Missing in isoform 2.
VSP_008910

Experimental info

Sequence conflict851V → D in AAA67356. Ref.1
Sequence conflict921A → AK in AAA67356. Ref.1
Sequence conflict4301W → L in AAA67356. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 2, 2002. Version 2.
Checksum: 251CACF6464B046B

FASTA73480,451
        10         20         30         40         50         60 
MSMMTVWALR RNVRRKNHSM LVRYISGSAS MKPKEQCIEK ILVANRGEIA CRIMRTAKRL 

        70         80         90        100        110        120 
GIQTVAVYSD ADRDSLHVKS ADEAVRIGPP SARLSYLSGV TIMEAAARTG AQAIHPGYGF 

       130        140        150        160        170        180 
LSESSDFAQL CEDSGLTFIG PPASAIRDMG DKSASKRIMG AAGVPLVPGY HGHEQDIDHM 

       190        200        210        220        230        240 
KSEAEKIGYP IIIKPTHGGG GKGMRIVQSG KDFADSFLGA QREAAASFGV NTILLEKYIT 

       250        260        270        280        290        300 
RPRHIEVQIF GDKHGNVLHL YERDCSVQRR HQKIIEEAPA PNISEKFRAN LGQAAVSAAR 

       310        320        330        340        350        360 
AVGYYNAGTV EFIVDTESDQ FYFMEMNTRL QVEHPVTEMI VGQDLVEWQI RVANGEPLPL 

       370        380        390        400        410        420 
SQSEVPMSGH AFEARIYAEN VPKGFLPATG VLNHYRPVAV SPSVRVETGV EQGDTVSMHY 

       430        440        450        460        470        480 
DPMIAKLVVW GGNRGEALVK LKDCLSNFQV AGVPTNINFL QKLASHKEFA VGNVETHFIE 

       490        500        510        520        530        540 
HHKSDLFADE SNPAATEVAY KAVKHSAALV AACISTIEHS TWNESNHGKV PSIWYSNPPF 

       550        560        570        580        590        600 
RVHHEAKQTI ELEWNNECEG TGSNLISLGV RYQPDGSYLI EEGNDSPSLE LRVTRAGKCD 

       610        620        630        640        650        660 
FRVEAAGLSM NVSLAAYLKD GYKHIHIWHG SEHHQFKQKV GIEFSEDEEG VQHRTSSETS 

       670        680        690        700        710        720 
SHPPGTIVAP MAGLVVKVLV ENEAKVDQGQ PILVLEAMKM EHVVKAPSSG SIQDLKVKAG 

       730 
QQVSDGSALF RIKG

« Hide

Isoform 2 [UniParc].

Checksum: 60AE5054E7C1C9E2
Show »

FASTA71478,369

References

« Hide 'large scale' references
[1]"Molecular cloning of the biotinylated subunit of 3-methylcrotonyl-coenzyme A carboxylase of Arabidopsis thaliana."
Weaver L.M., Lebrun L., Franklin A., Huang L., Hoffman N., Wurtele E.S., Nikolau B.J.
Plant Physiol. 107:1013-1014(1995) [PubMed: 7716229] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[5]"Molecular characterization of the non-biotin-containing subunit of 3-methylcrotonyl-CoA carboxylase."
McKean A.L., Ke J., Song J., Che P., Achenbach S., Nikolau B.J., Wurtele E.S.
J. Biol. Chem. 275:5582-5590(2000) [PubMed: 10681539] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: cv. Columbia and cv. Landsberg erecta.
[6]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-645, MASS SPECTROMETRY.
Strain: cv. Columbia.
Tissue: Seedling.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U12536 mRNA. Translation: AAA67356.1.
AC006550 Genomic DNA. Translation: AAD25800.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27527.1.
CP002684 Genomic DNA. Translation: AEE27528.1.
AY070723 mRNA. Translation: AAL50065.1.
IPIIPI00539641.
IPI00544747.
PIRG86161.
RefSeqNP_563674.1. NM_100191.3.
NP_849583.1. NM_179252.2.
UniGeneAt.24059.

3D structure databases

ProteinModelPortalQ42523.
SMRQ42523. Positions 38-733.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ42523.

Proteomic databases

PRIDEQ42523.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G03090.2; AT1G03090.2; AT1G03090.
GeneID838362.
GenomeReviewsGene locus AT1G03090 in contig CT485782_GR.
KEGGath:AT1G03090.
NMPDRfig|3702.1.peg.446.

Organism-specific databases

TAIRAt1g03090.

Phylogenomic databases

eggNOGKOG0238.
GeneTreeEPGT00070000030014.
HOGENOMHBG535236.
InParanoidQ42523.
OMAARIYAEH.
PhylomeDBQ42523.
ProtClustDBCLSN2916952.

Enzyme and pathway databases

BRENDA6.4.1.4. 401.

Gene expression databases

ArrayExpressQ42523.
GenevestigatorQ42523.
GermOnlineAT1G03090. Arabidopsis thaliana.

Family and domain databases

InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005479. CarbamoylP_synth_lsu_ATP-bd.
IPR005481. CarbamoylP_synth_lsu_N.
IPR013817. Pre-ATP_grasp.
IPR016185. PreATP-grasp-like.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
Gene3DG3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
KOK01968.
PfamPF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMSSF51230. Hybrid_motif. 1 hit.
SSF52440. PreATP-grasp-like. 1 hit.
SSF51246. Rudmnt_hyb_motif. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS00866. CPSASE_1. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMCCA_ARATH
AccessionPrimary (citable) accession number: Q42523
Secondary accession number(s): Q9SA61
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: December 14, 2011
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents