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Q42522 (GSA2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic

Short name=GSA 2
EC=5.4.3.8
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 2
Short name=GSA-AT 2
Gene names
Name:GSA2
Synonyms:HEML2
Ordered Locus Names:At3g48730
ORF Names:T8P19.240
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transaminase converting glutamate 1-semialdehyde (GSA) to 5-aminolevulinate (ALA). Involved in the biosynthesis of tetrapyrroles By similarity. HAMAP-Rule MF_00375

Catalytic activity

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375

Cofactor

Pyridoxal phosphate.

Pathway

Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375

Porphyrin-containing compound metabolism; chlorophyll biosynthesis. HAMAP-Rule MF_00375

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00375

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00375.

Sequence similarities

Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3636Chloroplast By similarity
Chain37 – 472436Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic HAMAP-Rule MF_00375
PRO_0000001256

Amino acid modifications

Modified residue3121N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict161C → S in AAA79123. Ref.1
Sequence conflict1941N → K in AAA79123. Ref.1
Sequence conflict2831F → L in AAA79123. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q42522 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: E463699500B353FE

FASTA47250,142
        10         20         30         40         50         60 
MAATLTGSGI ALGFSCSAKF SKRASSSSNR RCIKMSVSVE EKTKKFTLQK SEEAFNAAKN 

        70         80         90        100        110        120 
LMPGGVNSPV RAFKSVGGQP VVMDSAKGSR IRDIDGNEYI DYVGSWGPAI IGHADDEVLA 

       130        140        150        160        170        180 
ALAETMKKGT SFGAPCLLEN VLAEMVISAV PSIEMVRFVN SGTEACMGVL RLARAFTGKQ 

       190        200        210        220        230        240 
KFIKFEGCYH GHANSFLVKA GSGVATLGLP DSPGVPKAAT SDTLTAPYND IAAVEKLFEA 

       250        260        270        280        290        300 
NKGEIAAIIL EPVVGNSGFI TPKPEFIEGI RRITKDNGAL LIFDEVMTGF RLAYGGAQEY 

       310        320        330        340        350        360 
FGITPDLTTL GKIIGGGLPV GAYGGRRDIM EMVAPAGPMY QAGTLSGNPL AMTAGIHTLK 

       370        380        390        400        410        420 
RLSQPGTYEY LDKITKELTN GILEAGKKTG HAMCGGYISG MFGFFFTEGP VYDFSDAKKS 

       430        440        450        460        470 
DTEKFGKFFR GMLEEGVYLA PSQFEAGFTS LAHTSEDIQF TIAAAEKVLS RL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a gene encoding glutamate 1-semialdehyde aminotransferase from Arabidopsis thaliana 'Columbia'."
Wenzlau J.M., Berry-Lowe S.L.
Plant Gene Register PGR95-007
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Arabidopsis ORF clones."
Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.
Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10278 Genomic DNA. Translation: AAA79123.1.
AL133315 Genomic DNA. Translation: CAB62362.1.
CP002686 Genomic DNA. Translation: AEE78450.1.
BT025324 mRNA. Translation: ABF57280.1.
AK229328 mRNA. Translation: BAF01191.1.
PIRT46217.
RefSeqNP_190442.1. NM_114732.4.
UniGeneAt.19963.

3D structure databases

ProteinModelPortalQ42522.
SMRQ42522. Positions 50-472.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid9352. 1 interaction.
MINTMINT-8069640.
STRING3702.AT3G48730.1-P.

Proteomic databases

PaxDbQ42522.
PRIDEQ42522.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G48730.1; AT3G48730.1; AT3G48730.
GeneID824034.
KEGGath:AT3G48730.

Organism-specific databases

TAIRAT3G48730.

Phylogenomic databases

eggNOGCOG0001.
HOGENOMHOG000020210.
InParanoidQ42522.
KOK01845.
OMACGHANER.
PhylomeDBQ42522.
ProtClustDBPLN02482.

Enzyme and pathway databases

BioCycARA:AT3G48730-MONOMER.
UniPathwayUPA00251; UER00317.
UPA00668.

Gene expression databases

GenevestigatorQ42522.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPMF_00375. HemL_aminotrans_3.
InterProIPR004639. 4pyrrol_synth_GluAld_NH2Trfase.
IPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERPTHR11986. PTHR11986. 1 hit.
PfamPF00202. Aminotran_3. 1 hit.
[Graphical view]
PIRSFPIRSF000521. Transaminase_4ab_Lys_Orn. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
TIGRFAMsTIGR00713. hemL. 1 hit.
PROSITEPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSA2_ARATH
AccessionPrimary (citable) accession number: Q42522
Secondary accession number(s): Q1JPM9, Q9SMM6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names