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Protein

Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic

Gene

GSA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transaminase converting glutamate 1-semialdehyde (GSA) to 5-aminolevulinate (ALA). Involved in the biosynthesis of tetrapyrroles (By similarity).By similarity

Catalytic activityi

(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.

Cofactori

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase (AXX17_At1g09980), Glutamyl-tRNA reductase 2, chloroplastic (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase (AXX17_At1g52270), Glutamyl-tRNA reductase (AXX17_At2g27450), Probable glutamyl-tRNA reductase 3, chloroplastic (HEMA3)
  2. Glutamate-1-semialdehyde 2,1-aminomutase 1, chloroplastic (GSA1), Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (GSA2)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Pathwayi: chlorophyll biosynthesis

This protein is involved in the pathway chlorophyll biosynthesis, which is part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the pathway chlorophyll biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT3G48730-MONOMER
UniPathwayiUPA00251; UER00317
UPA00668

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplastic (EC:5.4.3.8)
Short name:
GSA 2
Alternative name(s):
Glutamate-1-semialdehyde aminotransferase 2
Short name:
GSA-AT 2
Gene namesi
Name:GSA2
Synonyms:HEML2
Ordered Locus Names:At3g48730
ORF Names:T8P19.240
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G48730
TAIRilocus:2114520 AT3G48730

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36ChloroplastBy similarityAdd BLAST36
ChainiPRO_000000125637 – 472Glutamate-1-semialdehyde 2,1-aminomutase 2, chloroplasticAdd BLAST436

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei312N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ42522
PRIDEiQ42522

Expressioni

Gene expression databases

ExpressionAtlasiQ42522 baseline and differential
GenevisibleiQ42522 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi9352, 2 interactors
STRINGi3702.AT3G48730.1

Structurei

3D structure databases

ProteinModelPortaliQ42522
SMRiQ42522
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IPFV Eukaryota
COG0001 LUCA
HOGENOMiHOG000020210
InParanoidiQ42522
KOiK01845
OMAiHANSFLV
OrthoDBiEOG093608WJ
PhylomeDBiQ42522

Family and domain databases

CDDicd00610 OAT_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
HAMAPiMF_00375 HemL_aminotrans_3, 1 hit
InterProiView protein in InterPro
IPR004639 4pyrrol_synth_GluAld_NH2Trfase
IPR005814 Aminotrans_3
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00202 Aminotran_3, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR00713 hemL, 1 hit
PROSITEiView protein in PROSITE
PS00600 AA_TRANSFER_CLASS_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATLTGSGI ALGFSCSAKF SKRASSSSNR RCIKMSVSVE EKTKKFTLQK
60 70 80 90 100
SEEAFNAAKN LMPGGVNSPV RAFKSVGGQP VVMDSAKGSR IRDIDGNEYI
110 120 130 140 150
DYVGSWGPAI IGHADDEVLA ALAETMKKGT SFGAPCLLEN VLAEMVISAV
160 170 180 190 200
PSIEMVRFVN SGTEACMGVL RLARAFTGKQ KFIKFEGCYH GHANSFLVKA
210 220 230 240 250
GSGVATLGLP DSPGVPKAAT SDTLTAPYND IAAVEKLFEA NKGEIAAIIL
260 270 280 290 300
EPVVGNSGFI TPKPEFIEGI RRITKDNGAL LIFDEVMTGF RLAYGGAQEY
310 320 330 340 350
FGITPDLTTL GKIIGGGLPV GAYGGRRDIM EMVAPAGPMY QAGTLSGNPL
360 370 380 390 400
AMTAGIHTLK RLSQPGTYEY LDKITKELTN GILEAGKKTG HAMCGGYISG
410 420 430 440 450
MFGFFFTEGP VYDFSDAKKS DTEKFGKFFR GMLEEGVYLA PSQFEAGFTS
460 470
LAHTSEDIQF TIAAAEKVLS RL
Length:472
Mass (Da):50,142
Last modified:June 1, 2001 - v2
Checksum:iE463699500B353FE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16C → S in AAA79123 (Ref. 1) Curated1
Sequence conflicti194N → K in AAA79123 (Ref. 1) Curated1
Sequence conflicti283F → L in AAA79123 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10278 Genomic DNA Translation: AAA79123.1
AL133315 Genomic DNA Translation: CAB62362.1
CP002686 Genomic DNA Translation: AEE78450.1
BT025324 mRNA Translation: ABF57280.1
AK229328 mRNA Translation: BAF01191.1
PIRiT46217
RefSeqiNP_190442.1, NM_114732.5
UniGeneiAt.19963

Genome annotation databases

EnsemblPlantsiAT3G48730.1; AT3G48730.1; AT3G48730
GeneIDi824034
GrameneiAT3G48730.1; AT3G48730.1; AT3G48730
KEGGiath:AT3G48730

Similar proteinsi

Entry informationi

Entry nameiGSA2_ARATH
AccessioniPrimary (citable) accession number: Q42522
Secondary accession number(s): Q1JPM9, Q9SMM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health