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Protein

Alpha-amylase

Gene

Amy32b

Organism
Hordeum vulgare (Barley)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203NucleophileUniRule annotation1
Active sitei228Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
Gene namesi
Name:Amy32bImported
OrganismiHordeum vulgare (Barley)Imported
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23UniRule annotationAdd BLAST23
ChainiPRO_501060497524 – 421Alpha-amylaseUniRule annotationAdd BLAST398

Expressioni

Gene expression databases

ExpressionAtlasiQ42504 baseline and differential

Structurei

3D structure databases

ProteinModelPortaliQ42504
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 366AamyInterPro annotationAdd BLAST342
Domaini367 – 421Alpha-amyl_C2InterPro annotationAdd BLAST55

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiKOG0471 Eukaryota
COG0366 LUCA

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR012850 A-amylase_bs_C
IPR013775 A-amylase_pln
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07821 Alpha-amyl_C2, 1 hit
PF00128 Alpha-amylase, 1 hit
PIRSFiPIRSF001028 Alph-amls_plant, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00810 Alpha-amyl_C2, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Q42504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKNGNLCCF SLLLLLLAGL ASGHQVLFQG FNWESWKQSG GWYNMMMGKV
60 70 80 90 100
DDIAAAGVTH VWLPPPSHSV SNEGYMPGRL YDIDASKYGN AAELKSLIGA
110 120 130 140 150
LHGKGVQAIA DIVINHRCAD YKDNRGIYCI FEGGTSDGRL DWGPHMICRD
160 170 180 190 200
DTKYADGTAN LDTGADFAAA PDIDHLNDRV QRELKEWLLW LKSDLGFDAW
210 220 230 240 250
RLDFARGYSP EMAKVYIDGT SPSLAVAEVW DNMATGGDGK PNYDQDAHRQ
260 270 280 290 300
NLVNWVDKVG GAASAGMVFD FTTKGILNAA VEGELWRLID PQGKPPDVMG
310 320 330 340 350
WWPAKAVTFV DNHDTGSTQP MWPFPSDKVM QGYAYILTHP GTPCIFYDHF
360 370 380 390 400
FNWGFKDEIA ALVAIRKRNG ITATSALEIL MHEGDAYVAE IDGKVVVKIG
410 420
TRYDVGAVIP AGFATSAHGK D
Length:421
Mass (Da):46,102
Last modified:November 1, 1996 - v1
Checksum:i7E43D13A75ACBC56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15208 Genomic DNA Translation: AAA32935.1
X05166 Genomic DNA Translation: CAA28803.1

Entry informationi

Entry nameiQ42504_HORVU
AccessioniPrimary (citable) accession number: Q42504
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

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