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Protein

Alpha-amylase

Gene

Amy32b

Organism
Hordeum vulgare (Barley)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei203NucleophileUniRule annotation1
Active sitei228Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
Gene namesi
Name:Amy32bImported
OrganismiHordeum vulgare (Barley)Imported
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23UniRule annotationAdd BLAST23
ChainiPRO_501060497524 – 421Alpha-amylaseUniRule annotationAdd BLAST398

Structurei

3D structure databases

ProteinModelPortaliQ42504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 366AamyInterPro annotationAdd BLAST342
Domaini367 – 421Alpha-amyl_C2InterPro annotationAdd BLAST55

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.

Family and domain databases

InterProiView protein in InterPro
IPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Q42504-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKNGNLCCF SLLLLLLAGL ASGHQVLFQG FNWESWKQSG GWYNMMMGKV
60 70 80 90 100
DDIAAAGVTH VWLPPPSHSV SNEGYMPGRL YDIDASKYGN AAELKSLIGA
110 120 130 140 150
LHGKGVQAIA DIVINHRCAD YKDNRGIYCI FEGGTSDGRL DWGPHMICRD
160 170 180 190 200
DTKYADGTAN LDTGADFAAA PDIDHLNDRV QRELKEWLLW LKSDLGFDAW
210 220 230 240 250
RLDFARGYSP EMAKVYIDGT SPSLAVAEVW DNMATGGDGK PNYDQDAHRQ
260 270 280 290 300
NLVNWVDKVG GAASAGMVFD FTTKGILNAA VEGELWRLID PQGKPPDVMG
310 320 330 340 350
WWPAKAVTFV DNHDTGSTQP MWPFPSDKVM QGYAYILTHP GTPCIFYDHF
360 370 380 390 400
FNWGFKDEIA ALVAIRKRNG ITATSALEIL MHEGDAYVAE IDGKVVVKIG
410 420
TRYDVGAVIP AGFATSAHGK D
Length:421
Mass (Da):46,102
Last modified:November 1, 1996 - v1
Checksum:i7E43D13A75ACBC56
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15208 Genomic DNA. Translation: AAA32935.1.
X05166 Genomic DNA. Translation: CAA28803.1.

Similar proteinsi

Entry informationi

Entry nameiQ42504_HORVU
AccessioniPrimary (citable) accession number: Q42504
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: September 27, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)