ID   PETM_CHLRE              Reviewed;          99 AA.
AC   Q42496;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   27-MAR-2024, entry version 102.
DE   RecName: Full=Cytochrome b6-f complex subunit 7, chloroplastic;
DE   AltName: Full=Cytochrome b6-f complex 4 kDa subunit;
DE   AltName: Full=Cytochrome b6-f complex subunit PetM;
DE   AltName: Full=Cytochrome b6-f complex subunit VII;
DE   Flags: Precursor;
GN   Name=petM; Synonyms=petX;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 61-98.
RC   STRAIN=cw15;
RX   PubMed=8631873; DOI=10.1074/jbc.271.18.10667;
RA   de Vitry C., Breyton C., Pierre Y., Popot J.-L.;
RT   "The 4-kDa nuclear-encoded PetM polypeptide of the chloroplast cytochrome
RT   b6f complex. Nucleic acid and protein sequences, targeting signals,
RT   transmembrane topology.";
RL   J. Biol. Chem. 271:10667-10671(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CC-1928;
RX   PubMed=8616155; DOI=10.1016/0005-2728(95)00157-3;
RA   Ketchner S.L., Malkin R.;
RT   "Nucleotide sequence of the PetM gene encoding a 4 kDa subunit of the
RT   cytochrome b6f complex from Chlamydomonas reinhardtii.";
RL   Biochim. Biophys. Acta 1273:195-197(1996).
RN   [3]
RP   PROTEIN SEQUENCE OF 61-87, AND CHARACTERIZATION.
RC   STRAIN=WT12;
RX   PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA   Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT   "Purification and characterization of the cytochrome b6 f complex from
RT   Chlamydomonas reinhardtii.";
RL   J. Biol. Chem. 270:29342-29349(1995).
RN   [4]
RP   PROTEIN SEQUENCE OF 61-82.
RC   STRAIN=CC-124;
RA   Schmidt C.L., Malkin R.;
RT   "Low molecular weight subunits associated with the cytochrome b6f complexes
RT   from spinach and Chlamydomonas reinhardtii.";
RL   Photosyn. Res. 38:73-81(1993).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 61-99.
RX   PubMed=14647374; DOI=10.1038/nature02155;
RA   Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT   "An atypical haem in the cytochrome b(6)f complex.";
RL   Nature 426:413-418(2003).
CC   -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC       electron transfer between photosystem II (PSII) and photosystem I
CC       (PSI), cyclic electron flow around PSI, and state transitions.
CC   -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC       cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC       the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC       PetN. The complex functions as a dimer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Single-
CC       pass membrane protein.
CC   -!- SIMILARITY: Belongs to the PetM family. {ECO:0000305}.
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DR   EMBL; X92488; CAA63221.1; -; mRNA.
DR   EMBL; U36401; AAC49525.1; -; Genomic_DNA.
DR   PIR; T08070; T08070.
DR   RefSeq; XP_001694096.1; XM_001694044.1.
DR   PDB; 1Q90; X-ray; 3.10 A; M=61-99.
DR   PDBsum; 1Q90; -.
DR   AlphaFoldDB; Q42496; -.
DR   SMR; Q42496; -.
DR   IntAct; Q42496; 1.
DR   PaxDb; 3055-EDP03032; -.
DR   EnsemblPlants; PNW76141; PNW76141; CHLRE_12g546150v5.
DR   GeneID; 5719581; -.
DR   Gramene; PNW76141; PNW76141; CHLRE_12g546150v5.
DR   KEGG; cre:CHLRE_12g546150v5; -.
DR   eggNOG; ENOG502T01X; Eukaryota.
DR   HOGENOM; CLU_2323765_0_0_1; -.
DR   OrthoDB; 314617at2759; -.
DR   BioCyc; CHLAMY:CHLREDRAFT_147968-MONOMER; -.
DR   BioCyc; MetaCyc:CHLREDRAFT_147968-MONOMER; -.
DR   EvolutionaryTrace; Q42496; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009512; C:cytochrome b6f complex; IEA:InterPro.
DR   GO; GO:0070469; C:respirasome; IEA:UniProtKB-KW.
DR   HAMAP; MF_00396; Cytb6_f_PetM; 1.
DR   InterPro; IPR012595; PetM_cyt_b6/f_cplx_su7.
DR   Pfam; PF08041; PetM; 1.
DR   SUPFAM; SSF103441; PetM subunit of the cytochrome b6f complex; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Direct protein sequencing; Electron transport;
KW   Membrane; Plastid; Respiratory chain; Thylakoid; Transit peptide;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..60
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:7493968,
FT                   ECO:0000269|PubMed:8631873, ECO:0000269|Ref.4"
FT   CHAIN           61..99
FT                   /note="Cytochrome b6-f complex subunit 7, chloroplastic"
FT                   /id="PRO_0000023864"
FT   TOPO_DOM        61..67
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        89..99
FT                   /note="Stromal"
FT                   /evidence="ECO:0000255"
FT   HELIX           63..93
FT                   /evidence="ECO:0007829|PDB:1Q90"
SQ   SEQUENCE   99 AA;  9998 MW;  5B3E45315A701AE4 CRC64;
     MAMSIAARSA CCGVAAPRSS TVRVAAARPA VRPSLRTAGQ KAAPSRGVAT KAVNELAMIA
     GEAEFIAGTA LTMVGMTLVG LAIGFVLLRV ESLVEEGKI
//