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Protein

Disease resistance protein RPS2

Gene

RPS2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Disease resistance (R) protein that specifically recognizes the AvrRpt2 type III effector avirulence protein from Pseudomonas syringae. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. Acts via its interaction with RIN4, and probably triggers the plant resistance when RIN4 is degraded by AvrRpt2.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi182 – 1898ATP

GO - Molecular functioni

GO - Biological processi

  • defense response Source: TAIR
  • defense response to bacterium Source: TAIR
  • detection of bacterium Source: TAIR
  • plant-type hypersensitive response Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Hypersensitive response, Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Disease resistance protein RPS2
Alternative name(s):
Resistance to Pseudomonas syringae protein 2
Gene namesi
Name:RPS2
Ordered Locus Names:At4g26090
ORF Names:F20B18.200
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26090.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication

  • Note: Not a peripheral membrane protein. Has the biochemical properties of an integral membrane protein without an obvious integral membrane primary structure.1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 2519Missing in R2M1; abolishes disease resistance to AvrRpt2. Induces dominant negative effect. 1 PublicationAdd
BLAST
Mutagenesisi38 – 403LET → TEL in R2M2; abolishes disease resistance to AvrRpt2. Induces dominant negative effect. 1 Publication
Mutagenesisi182 – 1821G → A: Reduced interaction with NRP1. 1 Publication
Mutagenesisi188 – 1881K → L in R2M4; abolishes disease resistance to AvrRpt2. Does not display a dominant negative effect. 1 Publication
Mutagenesisi189 – 1891T → A: Reduced interaction with NRP1. 1 Publication
Mutagenesisi189 – 1891T → S in R2M4a; no effect. In R2M4c; abolishes disease resistance to AvrRpt2; when associated with S-190. Does not display a dominant negative effect. 1 Publication
Mutagenesisi190 – 1901T → S in R2M4b; no effect. In R2M4c; abolishes disease resistance to AvrRpt2; when associated with S-189. Does not display a dominant negative effect. 1 Publication
Mutagenesisi262 – 2632DD → TA in R2M5; abolishes disease resistance to AvrRpt2. Does not display a dominant negative effect. 1 Publication
Mutagenesisi276 – 2761P → L in 204C; abolishes disease resistance to AvrRpt2.
Mutagenesisi353 – 3531I → K: No effect. 1 Publication
Mutagenesisi412 – 4121A → V in 205C; abolishes disease resistance to AvrRpt2.
Mutagenesisi456 – 4561A → T in 209C; abolishes disease resistance to AvrRpt2.
Mutagenesisi558 – 5581P → L in 210C; abolishes disease resistance to AvrRpt2.
Mutagenesisi566 – 5661S → L in 206C; abolishes disease resistance to AvrRpt2.
Mutagenesisi597 – 5971P → S in 211C; abolishes disease resistance to AvrRpt2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 909909Disease resistance protein RPS2PRO_0000212718Add
BLAST

Proteomic databases

PaxDbiQ42484.
PRIDEiQ42484.

Expressioni

Gene expression databases

GenevisibleiQ42484. AT.

Interactioni

Subunit structurei

Interacts indirectly with RIN4. Found in a complex with AvrRpt2 and AvrB (PubMed:10849351, PubMed:12581526). Interacts with MORC1/CRT1 (PubMed:18191794). Binds to NRP1 (Ref. 16).4 Publications

Protein-protein interaction databases

BioGridi14002. 14 interactions.
DIPiDIP-53465N.
IntActiQ42484. 10 interactions.
STRINGi3702.AT4G26090.1.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FT5model-A135-477[»]
ProteinModelPortaliQ42484.
SMRiQ42484. Positions 178-265, 511-639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 440306NB-ARCAdd
BLAST
Repeati512 – 53322LRR 1Add
BLAST
Repeati534 – 55623LRR 2Add
BLAST
Repeati559 – 58022LRR 3Add
BLAST
Repeati582 – 60423LRR 4Add
BLAST
Repeati605 – 62723LRR 5Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili29 – 5830Sequence analysisAdd
BLAST

Domaini

The LRR repeats probably act as specificity determinant of pathogen recognition.By similarity
The coiled coil domain is essential for the resistance to AvrRpt2; the cultivars that do not display resistance showing specific variations in this region.

Sequence similaritiesi

Belongs to the disease resistance NB-LRR family.Curated
Contains 5 LRR (leucine-rich) repeats.Curated
Contains 1 NB-ARC domain.Curated

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4658. Eukaryota.
COG4886. LUCA.
InParanoidiQ42484.
KOiK13459.
OMAiLIKMEYL.
OrthoDBiEOG093602JL.
PhylomeDBiQ42484.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00931. NB-ARC. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q42484-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFISSLIVG CAQVLCESMN MAERRGHKTD LRQAITDLET AIGDLKAIRD
60 70 80 90 100
DLTLRIQQDG LEGRSCSNRA REWLSAVQVT ETKTALLLVR FRRREQRTRM
110 120 130 140 150
RRRYLSCFGC ADYKLCKKVS AILKSIGELR ERSEAIKTDG GSIQVTCREI
160 170 180 190 200
PIKSVVGNTT MMEQVLEFLS EEEERGIIGV YGPGGVGKTT LMQSINNELI
210 220 230 240 250
TKGHQYDVLI WVQMSREFGE CTIQQAVGAR LGLSWDEKET GENRALKIYR
260 270 280 290 300
ALRQKRFLLL LDDVWEEIDL EKTGVPRPDR ENKCKVMFTT RSIALCNNMG
310 320 330 340 350
AEYKLRVEFL EKKHAWELFC SKVWRKDLLE SSSIRRLAEI IVSKCGGLPL
360 370 380 390 400
ALITLGGAMA HRETEEEWIH ASEVLTRFPA EMKGMNYVFA LLKFSYDNLE
410 420 430 440 450
SDLLRSCFLY CALFPEEHSI EIEQLVEYWV GEGFLTSSHG VNTIYKGYFL
460 470 480 490 500
IGDLKAACLL ETGDEKTQVK MHNVVRSFAL WMASEQGTYK ELILVEPSMG
510 520 530 540 550
HTEAPKAENW RQALVISLLD NRIQTLPEKL ICPKLTTLML QQNSSLKKIP
560 570 580 590 600
TGFFMHMPVL RVLDLSFTSI TEIPLSIKYL VELYHLSMSG TKISVLPQEL
610 620 630 640 650
GNLRKLKHLD LQRTQFLQTI PRDAICWLSK LEVLNLYYSY AGWELQSFGE
660 670 680 690 700
DEAEELGFAD LEYLENLTTL GITVLSLETL KTLFEFGALH KHIQHLHVEE
710 720 730 740 750
CNELLYFNLP SLTNHGRNLR RLSIKSCHDL EYLVTPADFE NDWLPSLEVL
760 770 780 790 800
TLHSLHNLTR VWGNSVSQDC LRNIRCINIS HCNKLKNVSW VQKLPKLEVI
810 820 830 840 850
ELFDCREIEE LISEHESPSV EDPTLFPSLK TLRTRDLPEL NSILPSRFSF
860 870 880 890 900
QKVETLVITN CPRVKKLPFQ ERRTQMNLPT VYCEEKWWKA LEKDQPNEEL

CYLPRFVPN
Length:909
Mass (Da):104,641
Last modified:November 1, 1996 - v1
Checksum:iD279B6E30E49D640
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951I → V (PubMed:9670562).Curated
Sequence conflicti267 – 2671E → G (PubMed:9670562).Curated

Polymorphismi

The polymorphism between the different cultivars influence the specificity to the pathogen recognition. In cv. Po.1, KNO2, BG-4 and Zu-0, RPS2 does not confer resistance to AvrRpt2.2 Publications

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 481I → V in strain: cv. Po-1 and cv. Zu-0. 2 Publications
Natural varianti104 – 1041Y → C in strain: cv. BG-4, cv. KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti154 – 1541S → Y in strain: cv. Pog-0. 1 Publication
Natural varianti419 – 4191S → P in strain: cv. BG-4, cv. KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti439 – 4391H → N in strain: cv. BG-4, cv. KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti472 – 4721H → Y in strain: cv. BG-4, cv. KNO2, cv. Po-1 and cv. Zu-0. 2 Publications
Natural varianti515 – 5151V → A in strain: cv. Yo-0. 1 Publication
Natural varianti515 – 5151V → L in strain: cv. Ang-0, cv. Mt-0 and cv. RLD. 1 Publication
Natural varianti527 – 5271P → H in strain: cv. Ab-7, cv. Fm-17, cv. Gr-6 and cv. Hs-12. 1 Publication
Natural varianti544 – 5441S → R in strain: cv. Wu-0. 1 Publication
Natural varianti545 – 5451S → Y in strain: cv. BG-4, cv. KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti644 – 6441E → G in strain: cv. BG-4, KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti649 – 6491G → E in strain: cv. Wu-0. 1 Publication
Natural varianti649 – 6491G → Q in strain: cv. BG-4, cv. KNO2, cv. Po-1 and cv. Zu-0. 2 Publications
Natural varianti653 – 6531A → V in strain: cv. BG-4, cv. KNO2, cv. Po-1, cv. Wu-0 and cv. Zu-0. 2 Publications
Natural varianti699 – 6991E → D in strain: cv. Yo-0. 1 Publication
Natural varianti703 – 7031E → D in strain: cv. Ang-0, cv. BG-4, cv. Bur-0, cv. Co-1, cv. Ct-1, cv. D2-9, cv. G2-1, cv. Kas-1, cv. KNO2, cv. Mt-0, cv. Po-1, cv. Pog-0, cv. Pu-8, cv. RLD, cv. Tamm-17, cv. Tsu-0, cv. Wu-0, cv. Yo-0 and cv. Zu-0. 2 Publications
Natural varianti778 – 7781N → K in strain: cv. D2-9, cv. G2-1 and cv. Tsu-0. 1 Publication
Natural varianti785 – 7851L → V in strain: cv. Wu-0. 1 Publication
Natural varianti833 – 8331R → T in strain: cv. BG-4, cv. Ct-1, cv. Cvi-0, cv. D2-9, cv. G2-1, cv. Kas-1, cv. KNO2, cv. Po-1, cv. Pu-8, cv. Tsu-0, cv. Wu-0 and cv. Zu-0. 2 Publications

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14158 Genomic DNA. Translation: AAA21874.1.
U12860 mRNA. Translation: AAA50236.1.
AF368301 Genomic DNA. Translation: AAK38117.1.
AF487797 Genomic DNA. Translation: AAM90859.1.
AF487798 Genomic DNA. Translation: AAM90860.1.
AF487799 Genomic DNA. Translation: AAM90861.1.
AF487800 Genomic DNA. Translation: AAM90862.1.
AF487801 Genomic DNA. Translation: AAM90863.1.
AF487802 Genomic DNA. Translation: AAM90864.1.
AF487803 Genomic DNA. Translation: AAM90865.1.
AF487804 Genomic DNA. Translation: AAM90866.1.
AF487805 Genomic DNA. Translation: AAM90867.1.
AF487806 Genomic DNA. Translation: AAM90868.1.
AF487807 Genomic DNA. Translation: AAM90869.1.
AF487808 Genomic DNA. Translation: AAM90870.1.
AF487809 Genomic DNA. Translation: AAM90871.1.
AF487810 Genomic DNA. Translation: AAM90872.1.
AF487811 Genomic DNA. Translation: AAM90873.1.
AF487812 Genomic DNA. Translation: AAM90874.1.
AF487813 Genomic DNA. Translation: AAM90875.1.
AF487814 Genomic DNA. Translation: AAM90876.1.
AF487815 Genomic DNA. Translation: AAM90877.1.
AF487816 Genomic DNA. Translation: AAM90878.1.
AF487817 Genomic DNA. Translation: AAM90879.1.
AF487818 Genomic DNA. Translation: AAM90880.1.
AF487819 Genomic DNA. Translation: AAM90881.1.
AF487820 Genomic DNA. Translation: AAM90882.1.
AF487821 Genomic DNA. Translation: AAM90883.1.
AF487822 Genomic DNA. Translation: AAM90884.1.
AF487823 Genomic DNA. Translation: AAM90885.1.
AL049483 Genomic DNA. Translation: CAB39674.1.
AL161564 Genomic DNA. Translation: CAB79464.1.
CP002687 Genomic DNA. Translation: AEE85156.1.
AK117214 mRNA. Translation: BAC41890.1.
BT005972 mRNA. Translation: AAO64907.1.
U97217 Genomic DNA. Translation: AAC50025.1.
PIRiA54809.
RefSeqiNP_194339.1. NM_118742.2.
UniGeneiAt.46720.
At.69675.
At.75226.

Genome annotation databases

EnsemblPlantsiAT4G26090.1; AT4G26090.1; AT4G26090.
GeneIDi828715.
GrameneiAT4G26090.1; AT4G26090.1; AT4G26090.
KEGGiath:AT4G26090.

Cross-referencesi

Web resourcesi

NIB-LRRS

Functional and comparative genomics of disease resistance gene homologs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14158 Genomic DNA. Translation: AAA21874.1.
U12860 mRNA. Translation: AAA50236.1.
AF368301 Genomic DNA. Translation: AAK38117.1.
AF487797 Genomic DNA. Translation: AAM90859.1.
AF487798 Genomic DNA. Translation: AAM90860.1.
AF487799 Genomic DNA. Translation: AAM90861.1.
AF487800 Genomic DNA. Translation: AAM90862.1.
AF487801 Genomic DNA. Translation: AAM90863.1.
AF487802 Genomic DNA. Translation: AAM90864.1.
AF487803 Genomic DNA. Translation: AAM90865.1.
AF487804 Genomic DNA. Translation: AAM90866.1.
AF487805 Genomic DNA. Translation: AAM90867.1.
AF487806 Genomic DNA. Translation: AAM90868.1.
AF487807 Genomic DNA. Translation: AAM90869.1.
AF487808 Genomic DNA. Translation: AAM90870.1.
AF487809 Genomic DNA. Translation: AAM90871.1.
AF487810 Genomic DNA. Translation: AAM90872.1.
AF487811 Genomic DNA. Translation: AAM90873.1.
AF487812 Genomic DNA. Translation: AAM90874.1.
AF487813 Genomic DNA. Translation: AAM90875.1.
AF487814 Genomic DNA. Translation: AAM90876.1.
AF487815 Genomic DNA. Translation: AAM90877.1.
AF487816 Genomic DNA. Translation: AAM90878.1.
AF487817 Genomic DNA. Translation: AAM90879.1.
AF487818 Genomic DNA. Translation: AAM90880.1.
AF487819 Genomic DNA. Translation: AAM90881.1.
AF487820 Genomic DNA. Translation: AAM90882.1.
AF487821 Genomic DNA. Translation: AAM90883.1.
AF487822 Genomic DNA. Translation: AAM90884.1.
AF487823 Genomic DNA. Translation: AAM90885.1.
AL049483 Genomic DNA. Translation: CAB39674.1.
AL161564 Genomic DNA. Translation: CAB79464.1.
CP002687 Genomic DNA. Translation: AEE85156.1.
AK117214 mRNA. Translation: BAC41890.1.
BT005972 mRNA. Translation: AAO64907.1.
U97217 Genomic DNA. Translation: AAC50025.1.
PIRiA54809.
RefSeqiNP_194339.1. NM_118742.2.
UniGeneiAt.46720.
At.69675.
At.75226.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FT5model-A135-477[»]
ProteinModelPortaliQ42484.
SMRiQ42484. Positions 178-265, 511-639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14002. 14 interactions.
DIPiDIP-53465N.
IntActiQ42484. 10 interactions.
STRINGi3702.AT4G26090.1.

Proteomic databases

PaxDbiQ42484.
PRIDEiQ42484.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26090.1; AT4G26090.1; AT4G26090.
GeneIDi828715.
GrameneiAT4G26090.1; AT4G26090.1; AT4G26090.
KEGGiath:AT4G26090.

Organism-specific databases

TAIRiAT4G26090.

Phylogenomic databases

eggNOGiKOG4658. Eukaryota.
COG4886. LUCA.
InParanoidiQ42484.
KOiK13459.
OMAiLIKMEYL.
OrthoDBiEOG093602JL.
PhylomeDBiQ42484.

Miscellaneous databases

PROiQ42484.

Gene expression databases

GenevisibleiQ42484. AT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00931. NB-ARC. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPS2_ARATH
AccessioniPrimary (citable) accession number: Q42484
Secondary accession number(s): O82096
, Q8L3R0, Q8L3W3, Q8L4X9, Q8L4Y0, Q8L587, Q8L5B3, Q8LKZ8, Q8LKZ9, Q8LL00, Q8LL01, Q9ASP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.