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Protein

Calcium-dependent protein kinase 3

Gene

CPK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in signal transduction pathways that involve calcium as a second messenger. Functions in abscisic acid (ABA) regulation of guard cell S-type anion-and Ca2+-permeable channels and stomatal closure.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATPPROSITE-ProRule annotation
Active sitei202 – 2021Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 929ATPPROSITE-ProRule annotation
Calcium bindingi392 – 403121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi428 – 439122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi464 – 475123PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi499 – 510124PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • intracellular signal transduction Source: GO_Central
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein autophosphorylation Source: TAIR
  • regulation of anion channel activity Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G23650-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent protein kinase 3 (EC:2.7.11.1)
Alternative name(s):
Calcium-dependent protein kinase isoform CDPK6
Short name:
AtCDPK6
Gene namesi
Name:CPK3
Synonyms:CDPK6
Ordered Locus Names:At4g23650
ORF Names:F9D16.120
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G23650.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • membrane Source: TAIR
  • nucleus Source: GO_Central
  • plasma membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 529528Calcium-dependent protein kinase 3PRO_0000363330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiQ42479.
PRIDEiQ42479.

PTM databases

iPTMnetiQ42479.

Expressioni

Tissue specificityi

Expressed in both guard cells and mesophyll cells.1 Publication

Gene expression databases

GenevisibleiQ42479. AT.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
CAM6Q035092EBI-1235782,EBI-1236097
CAM7P592202EBI-1235782,EBI-1236031

GO - Molecular functioni

Protein-protein interaction databases

BioGridi13754. 20 interactions.
IntActiQ42479. 8 interactions.
STRINGi3702.AT4G23650.1.

Structurei

3D structure databases

ProteinModelPortaliQ42479.
SMRiQ42479. Positions 42-517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini78 – 336259Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini379 – 41436EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini415 – 45036EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini451 – 48636EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini487 – 52135EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni342 – 37231Autoinhibitory domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi6 – 5954Ser-richAdd
BLAST

Domaini

There is 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (342-372) inactivates kinase activity under calcium-free conditions (By similarity).By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDPK subfamily.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ42479.
KOiK13412.
OMAiCKSIAAR.
PhylomeDBiQ42479.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42479-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGHRHSKSKS SDPPPSSSSS SSGNVVHHVK PAGERRGSSG SGTVGSSGSG
60 70 80 90 100
TGGSRSTTST QQNGRILGRP MEEVRRTYEF GRELGRGQFG VTYLVTHKET
110 120 130 140 150
KQQVACKSIP TRRLVHKDDI EDVRREVQIM HHLSGHRNIV DLKGAYEDRH
160 170 180 190 200
SVNLIMELCE GGELFDRIIS KGLYSERAAA DLCRQMVMVV HSCHSMGVMH
210 220 230 240 250
RDLKPENFLF LSKDENSPLK ATDFGLSVFF KPGDKFKDLV GSAYYVAPEV
260 270 280 290 300
LKRNYGPEAD IWSAGVILYI LLSGVPPFWG ENETGIFDAI LQGQLDFSAD
310 320 330 340 350
PWPALSDGAK DLVRKMLKYD PKDRLTAAEV LNHPWIREDG EASDKPLDNA
360 370 380 390 400
VLSRMKQFRA MNKLKKMALK VIAENLSEEE IIGLKEMFKS LDTDNNGIVT
410 420 430 440 450
LEELRTGLPK LGSKISEAEI RQLMEAADMD GDGSIDYLEF ISATMHMNRI
460 470 480 490 500
EREDHLYTAF QFFDNDNSGY ITMEELELAM KKYNMGDDKS IKEIIAEVDT
510 520
DRDGKINYEE FVAMMKKGNP ELVPNRRRM
Length:529
Mass (Da):59,336
Last modified:November 1, 1996 - v1
Checksum:i290259B0F428D376
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti315 – 3151K → E in AAL38596 (PubMed:14593172).Curated
Sequence conflicti315 – 3151K → E in AAK96512 (PubMed:14593172).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20623 mRNA. Translation: AAA67654.1.
U20625 Genomic DNA. Translation: AAA67656.1.
AL035394 Genomic DNA. Translation: CAA23031.1.
AL161559 Genomic DNA. Translation: CAB79320.1.
CP002687 Genomic DNA. Translation: AEE84789.1.
AF385710 mRNA. Translation: AAK60302.1.
AF446863 mRNA. Translation: AAL38596.1.
AY052319 mRNA. Translation: AAK96512.1.
AY081732 mRNA. Translation: AAL87385.1.
BT000736 mRNA. Translation: AAN31878.1.
AY087637 mRNA. Translation: AAM65176.1.
PIRiT05597. S71774.
RefSeqiNP_194096.1. NM_118496.3.
UniGeneiAt.24517.

Genome annotation databases

EnsemblPlantsiAT4G23650.1; AT4G23650.1; AT4G23650.
GeneIDi828465.
GrameneiAT4G23650.1; AT4G23650.1; AT4G23650.
KEGGiath:AT4G23650.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U20623 mRNA. Translation: AAA67654.1.
U20625 Genomic DNA. Translation: AAA67656.1.
AL035394 Genomic DNA. Translation: CAA23031.1.
AL161559 Genomic DNA. Translation: CAB79320.1.
CP002687 Genomic DNA. Translation: AEE84789.1.
AF385710 mRNA. Translation: AAK60302.1.
AF446863 mRNA. Translation: AAL38596.1.
AY052319 mRNA. Translation: AAK96512.1.
AY081732 mRNA. Translation: AAL87385.1.
BT000736 mRNA. Translation: AAN31878.1.
AY087637 mRNA. Translation: AAM65176.1.
PIRiT05597. S71774.
RefSeqiNP_194096.1. NM_118496.3.
UniGeneiAt.24517.

3D structure databases

ProteinModelPortaliQ42479.
SMRiQ42479. Positions 42-517.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13754. 20 interactions.
IntActiQ42479. 8 interactions.
STRINGi3702.AT4G23650.1.

PTM databases

iPTMnetiQ42479.

Proteomic databases

PaxDbiQ42479.
PRIDEiQ42479.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G23650.1; AT4G23650.1; AT4G23650.
GeneIDi828465.
GrameneiAT4G23650.1; AT4G23650.1; AT4G23650.
KEGGiath:AT4G23650.

Organism-specific databases

TAIRiAT4G23650.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
HOGENOMiHOG000233030.
InParanoidiQ42479.
KOiK13412.
OMAiCKSIAAR.
PhylomeDBiQ42479.

Enzyme and pathway databases

BioCyciARA:AT4G23650-MONOMER.

Miscellaneous databases

PROiQ42479.

Gene expression databases

GenevisibleiQ42479. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13499. EF-hand_7. 2 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of three members of the calcium-dependent protein kinase gene family in Arabidopsis thaliana."
    Hong Y., Takano M., Liu C.-M., Gasch A., Chye M.-L., Chua N.-H.
    Plant Mol. Biol. 30:1259-1275(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Columbia.
  2. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "The CDPK superfamily of protein kinases."
    Harmon A.C., Gribskov M., Gubrium E., Harper J.F.
    New Phytol. 151:175-183(2001)
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family."
    Cheng S.-H., Willmann M.R., Chen H.-C., Sheen J.
    Plant Physiol. 129:469-485(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  8. Cited for: GENE FAMILY, NOMENCLATURE.
  9. "Subcellular targeting of nine calcium-dependent protein kinase isoforms from Arabidopsis."
    Dammann C., Ichida A., Hong B., Romanowsky S.M., Hrabak E.M., Harmon A.C., Pickard B.G., Harper J.F.
    Plant Physiol. 132:1840-1848(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  10. "CDPKs CPK6 and CPK3 function in ABA regulation of guard cell S-type anion-and Ca(2+)-permeable channels and stomatal closure."
    Mori I.C., Murata Y., Yang Y., Munemasa S., Wang Y.-F., Andreoli S., Tiriac H., Alonso J.M., Harper J.F., Ecker J.R., Kwak J.M., Schroeder J.I.
    PLoS Biol. 4:E327-E327(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  11. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCDPK3_ARATH
AccessioniPrimary (citable) accession number: Q42479
Secondary accession number(s): Q941A2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.