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Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the production of GABA. The calmodulin-binding is calcium-dependent and it is proposed that this may, directly or indirectly, form a calcium regulated control of GABA biosynthesis.1 Publication

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Enzyme regulationi

Up-regulatd by calmodulin binding at physiological pH.1 Publication

pH dependencei

Optimum pH is 6.0.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei488Anchoring site for calmodulin binding; modulates the equilibrium between pyridoxal phosphate tautomersBy similarity1
Sitei489Anchoring site for calmodulin binding; modulates the equilibrium between pyridoxal phosphate tautomersBy similarity1

GO - Molecular functioni

  • glutamate decarboxylase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • glutamate metabolic process Source: TAIR
  • nitrogen compound metabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Ligandi

Calmodulin-binding, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:AT1G65960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Short name:
GAD 2
Gene namesi
Name:GAD2
Synonyms:GDH2
Ordered Locus Names:At1g65960
ORF Names:F12P19.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G65960.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001469741 – 494Glutamate decarboxylase 2Add BLAST494

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PaxDbiQ42472.
PRIDEiQ42472.

PTM databases

iPTMnetiQ42472.

Expressioni

Tissue specificityi

Expressed in roots, inflorescence stems, flowers, siliques and leaves.3 Publications

Inductioni

Up-regulated by salt or nitrogen treatments. Down-regulated by hypoxia.3 Publications

Gene expression databases

ExpressionAtlasiQ42472. baseline and differential.
GenevisibleiQ42472. AT.

Interactioni

Subunit structurei

Homohexamer (By similarity). Interacts with calmodulin.By similarity1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G65960.2.

Structurei

3D structure databases

ProteinModelPortaliQ42472.
SMRiQ42472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni463 – 494Calmodulin-bindingAdd BLAST32

Domaini

The C-terminus (463-494) binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiKOG1383. Eukaryota.
COG0076. LUCA.
HOGENOMiHOG000070228.
InParanoidiQ42472.
KOiK01580.
OMAiMENCIEN.
OrthoDBiEOG093607ZR.
PhylomeDBiQ42472.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42472-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLTKTATND ESVCTMFGSR YVRTTLPKYE IGENSIPKDA AYQIIKDELM
60 70 80 90 100
LDGNPRLNLA SFVTTWMEPE CDKLIMDSIN KNYVDMDEYP VTTELQNRCV
110 120 130 140 150
NIIARLFNAP LEESETAVGV GTVGSSEAIM LAGLAFKRKW QNKRKAEGKP
160 170 180 190 200
YDKPNIVTGA NVQVCWEKFA RYFEVELKEV NLSEGYYVMD PDKAAEMVDE
210 220 230 240 250
NTICVAAILG STLNGEFEDV KRLNDLLVKK NEETGWNTPI HVDAASGGFI
260 270 280 290 300
APFIYPELEW DFRLPLVKSI NVSGHKYGLV YAGIGWVVWR AAEDLPEELI
310 320 330 340 350
FHINYLGADQ PTFTLNFSKG SSQIIAQYYQ LIRLGFEGYK NVMENCIENM
360 370 380 390 400
VVLKEGIEKT ERFNIVSKDQ GVPVVAFSLK DHSFHNEFEI SEMLRRFGWI
410 420 430 440 450
VPAYTMPADA QHITVLRVVI REDFSRTLAE RLVADISKVL HELDTLPSKI
460 470 480 490
SKKMGIEGIA ENVKEKKMEK EILMEVIVGW RKFVKERKKM NGVC
Length:494
Mass (Da):56,141
Last modified:November 1, 1997 - v1
Checksum:i741E83A25DCBC48C
GO

Sequence cautioni

The sequence AAL91148 differs from that shown. Reason: Frameshift at position 70.Curated
The sequence AAL91148 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti410A → V in AAL16126 (PubMed:14593172).Curated1
Sequence conflicti461E → K in AAL91148 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49937 mRNA. Translation: AAC31617.1.
U46665 mRNA. Translation: AAC33485.1.
AC009513 Genomic DNA. Translation: AAF06056.1.
CP002684 Genomic DNA. Translation: AEE34445.1.
AF428294 mRNA. Translation: AAL16126.1.
AF428372 mRNA. Translation: AAL16302.1.
AY124873 mRNA. Translation: AAM70582.1.
AY081259 mRNA. Translation: AAL91148.1. Sequence problems.
PIRiH96683.
RefSeqiNP_001117556.1. NM_001124084.1. [Q42472-1]
UniGeneiAt.19149.
At.20543.
At.24993.
At.66846.

Genome annotation databases

EnsemblPlantsiAT1G65960.2; AT1G65960.2; AT1G65960. [Q42472-1]
GeneIDi842908.
GrameneiAT1G65960.2; AT1G65960.2; AT1G65960.
KEGGiath:AT1G65960.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49937 mRNA. Translation: AAC31617.1.
U46665 mRNA. Translation: AAC33485.1.
AC009513 Genomic DNA. Translation: AAF06056.1.
CP002684 Genomic DNA. Translation: AEE34445.1.
AF428294 mRNA. Translation: AAL16126.1.
AF428372 mRNA. Translation: AAL16302.1.
AY124873 mRNA. Translation: AAM70582.1.
AY081259 mRNA. Translation: AAL91148.1. Sequence problems.
PIRiH96683.
RefSeqiNP_001117556.1. NM_001124084.1. [Q42472-1]
UniGeneiAt.19149.
At.20543.
At.24993.
At.66846.

3D structure databases

ProteinModelPortaliQ42472.
SMRiQ42472.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G65960.2.

PTM databases

iPTMnetiQ42472.

Proteomic databases

PaxDbiQ42472.
PRIDEiQ42472.

Protocols and materials databases

DNASUi842908.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G65960.2; AT1G65960.2; AT1G65960. [Q42472-1]
GeneIDi842908.
GrameneiAT1G65960.2; AT1G65960.2; AT1G65960.
KEGGiath:AT1G65960.

Organism-specific databases

TAIRiAT1G65960.

Phylogenomic databases

eggNOGiKOG1383. Eukaryota.
COG0076. LUCA.
HOGENOMiHOG000070228.
InParanoidiQ42472.
KOiK01580.
OMAiMENCIEN.
OrthoDBiEOG093607ZR.
PhylomeDBiQ42472.

Enzyme and pathway databases

BioCyciARA:AT1G65960-MONOMER.

Miscellaneous databases

PROiQ42472.

Gene expression databases

ExpressionAtlasiQ42472. baseline and differential.
GenevisibleiQ42472. AT.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDCE2_ARATH
AccessioniPrimary (citable) accession number: Q42472
Secondary accession number(s): Q8RXH0, Q944L6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.