Q42456 (ASPR1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartic proteinase oryzasin-1
EC=3.4.23.-

Gene names

Ordered Locus Names:	Os05g0567100, LOC_Os05g49200
ORF Names:	OJ1781_H11.11

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	509 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Developmental stage	Seed ripening and germination.
Sequence similarities	Belongs to the peptidase A1 family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 67	47	Activation peptide Potential		PRO_0000025908
	Chain	68 – 509	442	Aspartic proteinase oryzasin-1		PRO_0000025909
Regions
	Domain	315 – 420	106	Saposin B-type
Sites
	Active site	103	1	By similarity
	Active site	290	1	By similarity
Amino acid modifications
	Glycosylation	252	1	N-linked (GlcNAc...) Potential
	Glycosylation	400	1	N-linked (GlcNAc...) Potential
	Disulfide bond	116 ↔ 122		By similarity
	Disulfide bond	281 ↔ 285		By similarity
	Disulfide bond	428 ↔ 465		By similarity
Experimental info
	Sequence conflict	25	1	A → E in BAA06875. Ref.1
	Sequence conflict	25	1	A → E in BAA06876. Ref.1
	Sequence conflict	443	1	G → A in BAA06875. Ref.1
	Sequence conflict	443	1	G → A in BAA06876. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q42456 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 6316EB933C5749C2	FASTA	509	54,074
	10 20 30 40 50 60 MGTRSVALVL LAAVLLQALL PASAAEGLVR IALKKRPIDE NSRVAARLSG EEGARRLGLR 70 80 90 100 110 120 GANSLGGGGG EGDIVALKNY MNAQYFGEIG VGTPPQKFTV IFDTGSSNLW VPSAKCYFSI 130 140 150 160 170 180 ACFFHSRYKS GQSSTYQKNG KPAAIQYGTG SIAGFFSEDS VTVGDLVVKD QEFIEATKEP 190 200 210 220 230 240 GLTFMVAKFD GILGLGFQEI SVGDAVPVWY KMVEQGLVSE PVFSFWFNRH SDEGEGGEIV 250 260 270 280 290 300 FGGMDPSHYK GNHTYVPVSQ KGYWQFEMGD VLIGGKTTGF CASGCSAIAD SGTSLLAGPT 310 320 330 340 350 360 AIITEINEKI GATGVVSQEC KTVVSQYGQQ ILDLLLAETQ PSKICSQVGL CTFDGKHGVS 370 380 390 400 410 420 AGIKSVVDDE AGESNGLQSG PMCNACEMAV VWMQNQLAQN KTQDLILNYI NQLCDKLPSP 430 440 450 460 470 480 MGESSVDCGS LASMPEISFT IGGKKFALKP EEYILKVGEG AAAQCISGFT AMDIPPPRGP 490 500 LWILGDVFMG AYHTVFDYGK MRVGFAKSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rice aspartic proteinase, oryzasin, expressed during seed ripening and germination, has a gene organization distinct from those of animal and microbial aspartic proteinases." Asakura T., Watanabe H., Abe K., Arai S. Eur. J. Biochem. 232:77-83(1995) [PubMed: 7556174] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Nipponbare. Tissue: Seed.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D32144 mRNA. Translation: BAA06875.1. D32165 Genomic DNA. Translation: BAA06876.1. AC120986 Genomic DNA. Translation: AAU10663.1.
PIR	S66516.
RefSeq	NP_001056348.1. NM_001062883.1.
UniGene	Os.8298.
3D structure databases
ProteinModelPortal	Q42456.
SMR	Q42456. Positions 29-509.
ModBase	Search...
Protein-protein interaction databases
STRING	Q42456.
Protein family/group databases
MEROPS	A01.020.
Proteomic databases
PRIDE	Q42456.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g49200.1; LOC_Os05g49200.1; LOC_Os05g49200. LOC_Os05g49200.2; LOC_Os05g49200.2; LOC_Os05g49200.
GeneID	4339639.
KEGG	osa:4339639.
Organism-specific databases
Gramene	Q42456.
Phylogenomic databases
GeneTree	EPGT00070000028304.
OMA	PSISVAN.
PhylomeDB	Q42456.
ProtClustDB	CLSN2919043.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR021109. Peptidase_aspartic. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR007856. SapB_1. IPR008138. SapB_2. IPR011001. Saposin-like. IPR008139. SaposinB. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 3 hits. G3DSA:1.10.225.10. Saposin_like. 2 hits.
KO	K08245.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. PF05184. SapB_1. 1 hit. PF03489. SapB_2. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SMART	SM00741. SapB. 1 hit. [Graphical view]
SUPFAM	SSF50630. Pept_Aspartic. 1 hit. SSF47862. Saposin_like. 1 hit.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. PS50015. SAP_B. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPR1_ORYSJ

Accession

Primary (citable) accession number: Q42456
Secondary accession number(s): Q688W6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	October 25, 2005
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program