Q42449 (PROF1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Profilin-1

Alternative name(s):
Allergen=Ara t 8

Gene names

Name:	PRO1
Synonyms:	PFN1, PRF1
Ordered Locus Names:	At2g19760
ORF Names:	F6F22.21

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG By similarity.
Subunit structure	Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Tissue specificity	Ubiquitous.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the profilin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Disease	Allergen
Ligand	Actin-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin polymerization or depolymerization Inferred from direct assay PubMed 8647830. Source: TAIR unidimensional cell growth Inferred from mutant phenotype PubMed 11340190. Source: TAIR
Cellular_component	apoplast Inferred from direct assay PubMed 21798377. Source: TAIR cell wall Inferred from direct assay PubMed 15469496. Source: TAIR cytosol Inferred from direct assay PubMed 21166475. Source: TAIR nucleolus Inferred from direct assay PubMed 15469496. Source: TAIR phragmoplast Inferred from direct assay PubMed 15469496. Source: TAIR plasma membrane Inferred from direct assay PubMed 17644812. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR spindle Inferred from direct assay PubMed 15469496. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 131

130

Profilin-1

PRO_0000199615

Secondary structure

1

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131

Helix Strand Turn

Details...

Helix

3 – 9

7

Beta strand

21 – 27

7

Beta strand

32 – 35

4

Helix

44 – 55

12

Turn

61 – 63

3

Beta strand

65 – 67

3

Beta strand

70 – 78

9

Turn

79 – 81

3

Beta strand

82 – 87

6

Beta strand

90 – 96

7

Beta strand

98 – 106

9

Helix

112 – 128

17

Sequences

	Sequence		Length	Mass (Da)
	Q42449 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 84212D4681F83F51	FASTA	131	14,266
	10 20 30 40 50 60 MSWQSYVDDH LMCDVEGNHL TAAAILGQDG SVWAQSAKFP QLKPQEIDGI KKDFEEPGFL 70 80 90 100 110 120 APTGLFLGGE KYMVIQGEQG AVIRGKKGPG GVTIKKTNQA LVFGFYDEPM TGGQCNLVVE 130 RLGDYLIESE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arabidopsis profilins are functionally similar to yeast profilins: identification of a vascular bundle-specific profilin and a pollen-specific profilin." Christensen H.E.M., Ramachandran S., Tan C.T., Surana U., Dong C.H., Chua N.-H. Plant J. 10:269-279(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[2]	"The Arabidopsis profilin gene family. Evidence for an ancient split between constitutive and pollen-specific profilin genes." Huang S., McDowell J.M., Weise M.J., Meagher R.B. Plant Physiol. 111:115-126(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The crystal structure of a major allergen from plants." Thorn K.S., Christensen H.E.M., Shigeta R. Jr., Hudler D. Jr., Shalaby L., Lindnerg U., Chua N.-H., Schutt C.E. Structure 5:19-32(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U43325 mRNA. Translation: AAB39480.1.
U43322 Genomic DNA. Translation: AAB39476.1.
U43593 Genomic DNA. Translation: AAG10090.1.
U43590 mRNA. Translation: AAB46750.1.
AC005169 Genomic DNA. Translation: AAC62140.1.
CP002685 Genomic DNA. Translation: AEC06923.1.
AY072427 mRNA. Translation: AAL62419.1.
BT000264 mRNA. Translation: AAN15583.1.

IPI

IPI00540256.

PIR

G84580.

RefSeq

NP_179566.1. NM_127534.2.

UniGene

At.23712.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A0K	X-ray	2.20	A	1-131	[»]
1PLM	model	-	A	2-131	[»]
3NUL	X-ray	1.60	A	2-131	[»]

ProteinModelPortal

Q42449.

SMR

Q42449. Positions 2-131.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q42449. 1 interaction.

STRING

3702.AT2G19760.1-P.

Proteomic databases

PaxDb

Q42449.

PRIDE

Q42449.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT2G19760.1; AT2G19760.1; AT2G19760.

GeneID

816495.

KEGG

ath:AT2G19760.

Organism-specific databases

TAIR

At2g19760.

Phylogenomic databases

eggNOG

NOG277929.

HOGENOM

HOG000171591.

InParanoid

Q42449.

KO

K05759.

OMA

KPQEIDG.

PhylomeDB

Q42449.

ProtClustDB

CLSN2912971.

Gene expression databases

Genevestigator

Q42449.

GermOnline

AT2G19760. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR027310. Profilin_CS.
IPR005455. Profilin_eukaryotes/bac.
[Graphical view]

PANTHER

PTHR11604. PTHR11604. 1 hit.

Pfam

PF00235. Profilin. 1 hit.
[Graphical view]

PRINTS

PR00392. PROFILIN.
PR01640. PROFILINPLNT.

SMART

SM00392. PROF. 1 hit.
[Graphical view]

SUPFAM

SSF55770. Profilin. 1 hit.

PROSITE

PS00414. PROFILIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q42449.

Entry information

Entry name

PROF1_ARATH

Accession

Primary (citable) accession number: Q42449

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program