Q42440 (PAI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic
EC=5.3.1.24

Gene names

Name:	PAI1
Synonyms:	TRP6
Ordered Locus Names:	At1g07780
ORF Names:	F24B9.11

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.
Subcellular location	Plastid › chloroplast Ref.8 Ref.9.
Tissue specificity	Expressed in roots and shoots. Ref.7
Induction	By silver nitrate and UV irradiation. Ref.7
Disruption phenotype	Bushy morphology, reduced fertility, blue fluorescence under UV light and resistance to the anthranilate analog 6-methylanthranilate. Ref.7
Sequence similarities	Belongs to the TrpF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tryptophan biosynthetic process Inferred from mutant phenotype Ref.1. Source: TAIR
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphoribosylanthranilate isomerase activity Inferred from mutant phenotype Ref.2. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42440-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Chloroplast Potential
	Chain	33 – 275	243	N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic		PRO_0000417453

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5F4417E94255F2C6	FASTA	275	29,633
	10 20 30 40 50 60 MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN TENVAPKDDE 70 80 90 100 110 120 RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SKVAREGGAK 130 140 150 160 170 180 PVGVFVEDDE NTILRAADSS DLELVQLHGN GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE 190 200 210 220 230 240 VPEEDCHLAD WILVDSATGG SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ 250 260 270 PDGIDVSSGI CGTDGIQKDK SKISSFITAV RSVHY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis." Bender J., Fink G.R. Cell 83:725-734(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Wassilewskija.
[2]	"Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes." Li J., Zhao J., Rose A.B., Schmidt R., Last R.L. Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Arabidopsis PAI gene arrangements, cytosine methylation and expression." Melquist S., Luff B., Bender J. Genetics 153:401-413(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[7]	"Differential expression of triplicate phosphoribosylanthranilate isomerase isogenes in the tryptophan biosynthetic pathway of Arabidopsis thaliana (L.) Heynh." He Y., Li J. Planta 212:641-647(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE. Strain: cv. Wassilewskija.
[8]	"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins." Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N. Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering." Olinares P.D., Ponnala L., van Wijk K.J. Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34756 Genomic DNA. Translation: AAB03498.1. U18970 Genomic DNA. Translation: AAC49005.1. AF130878 Genomic DNA. Translation: AAD38141.1. AC007583 Genomic DNA. Translation: AAF75075.1. CP002684 Genomic DNA. Translation: AEE28179.1. CP002684 Genomic DNA. Translation: AEE28180.1. CP002684 Genomic DNA. Translation: AEE28181.1. CP002684 Genomic DNA. Translation: AEE28183.1. AY081274 mRNA. Translation: AAL91163.1. BT003351 mRNA. Translation: AAO29969.1.
IPI	IPI00532830.
PIR	C86213.
RefSeq	NP_001184931.1. NM_001198002.1. NP_172257.1. NM_100652.4. NP_849606.1. NM_179275.3. NP_973784.2. NM_202055.3.
UniGene	At.48165. At.48988.
3D structure databases
ProteinModelPortal	Q42440.
SMR	Q42440. Positions 68-273.
ModBase	Search...
Proteomic databases
PRIDE	Q42440.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G07780.1; AT1G07780.1; AT1G07780. AT1G07780.2; AT1G07780.2; AT1G07780. AT1G07780.3; AT1G07780.3; AT1G07780. AT1G07780.5; AT1G07780.5; AT1G07780.
GeneID	837292.
KEGG	ath:AT1G07780.
Organism-specific databases
TAIR	At1g07780.
Phylogenomic databases
InParanoid	Q42440.
KO	K01817.
OMA	FVNASRC.
ProtClustDB	PLN02363.
Enzyme and pathway databases
UniPathway	UPA00035; UER00042.
Gene expression databases
Genevestigator	Q42440.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR001240. PRAI_dom. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00697. PRAI. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PAI1_ARATH

Accession

Primary (citable) accession number: Q42440
Secondary accession number(s): Q3EDG7, Q7G9J9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2012
Last sequence update:	November 1, 1996
Last modified:	May 29, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program