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Q42440

- PAI1_ARATH

UniProt

Q42440 - PAI1_ARATH

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Protein

N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic

Gene

PAI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.

Pathwayi

GO - Molecular functioni

  1. phosphoribosylanthranilate isomerase activity Source: TAIR

GO - Biological processi

  1. tryptophan biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G07780-MONOMER.
ARA:GQT-133-MONOMER.
ARA:GQT-134-MONOMER.
ARA:GQT-135-MONOMER.
UniPathwayiUPA00035; UER00042.

Names & Taxonomyi

Protein namesi
Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (EC:5.3.1.24)
Gene namesi
Name:PAI1
Synonyms:TRP6
Ordered Locus Names:At1g07780
ORF Names:F24B9.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G07780.

Subcellular locationi

Plastidchloroplast 2 Publications

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Bushy morphology, reduced fertility, blue fluorescence under UV light and resistance to the anthranilate analog 6-methylanthranilate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232ChloroplastSequence AnalysisAdd
BLAST
Chaini33 – 275243N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplasticPRO_0000417453Add
BLAST

Proteomic databases

PRIDEiQ42440.

Expressioni

Tissue specificityi

Expressed in roots and shoots.1 Publication

Inductioni

By silver nitrate and UV irradiation.1 Publication

Gene expression databases

ExpressionAtlasiQ42440. baseline.
GenevestigatoriQ42440.

Structurei

3D structure databases

ProteinModelPortaliQ42440.
SMRiQ42440. Positions 68-273.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpF family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK01817.
OMAiPDYAGFV.
PhylomeDBiQ42440.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q42440-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN
60 70 80 90 100
TENVAPKDDE RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR
110 120 130 140 150
SISLSVAKDI SKVAREGGAK PVGVFVEDDE NTILRAADSS DLELVQLHGN
160 170 180 190 200
GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE VPEEDCHLAD WILVDSATGG
210 220 230 240 250
SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ PDGIDVSSGI
260 270
CGTDGIQKDK SKISSFITAV RSVHY
Length:275
Mass (Da):29,633
Last modified:November 1, 1996 - v1
Checksum:i5F4417E94255F2C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34756 Genomic DNA. Translation: AAB03498.1.
U18970 Genomic DNA. Translation: AAC49005.1.
AF130878 Genomic DNA. Translation: AAD38141.1.
AC007583 Genomic DNA. Translation: AAF75075.1.
CP002684 Genomic DNA. Translation: AEE28179.1.
CP002684 Genomic DNA. Translation: AEE28180.1.
CP002684 Genomic DNA. Translation: AEE28181.1.
CP002684 Genomic DNA. Translation: AEE28183.1.
AY081274 mRNA. Translation: AAL91163.1.
BT003351 mRNA. Translation: AAO29969.1.
PIRiC86213.
RefSeqiNP_001184931.1. NM_001198002.1. [Q42440-1]
NP_172257.1. NM_100652.4. [Q42440-1]
NP_849606.1. NM_179275.3. [Q42440-1]
NP_973784.2. NM_202055.3. [Q42440-1]
UniGeneiAt.48165.
At.48988.

Genome annotation databases

EnsemblPlantsiAT1G07780.1; AT1G07780.1; AT1G07780. [Q42440-1]
AT1G07780.2; AT1G07780.2; AT1G07780. [Q42440-1]
AT1G07780.3; AT1G07780.3; AT1G07780. [Q42440-1]
AT1G07780.5; AT1G07780.5; AT1G07780. [Q42440-1]
GeneIDi837292.
KEGGiath:AT1G07780.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U34756 Genomic DNA. Translation: AAB03498.1 .
U18970 Genomic DNA. Translation: AAC49005.1 .
AF130878 Genomic DNA. Translation: AAD38141.1 .
AC007583 Genomic DNA. Translation: AAF75075.1 .
CP002684 Genomic DNA. Translation: AEE28179.1 .
CP002684 Genomic DNA. Translation: AEE28180.1 .
CP002684 Genomic DNA. Translation: AEE28181.1 .
CP002684 Genomic DNA. Translation: AEE28183.1 .
AY081274 mRNA. Translation: AAL91163.1 .
BT003351 mRNA. Translation: AAO29969.1 .
PIRi C86213.
RefSeqi NP_001184931.1. NM_001198002.1. [Q42440-1 ]
NP_172257.1. NM_100652.4. [Q42440-1 ]
NP_849606.1. NM_179275.3. [Q42440-1 ]
NP_973784.2. NM_202055.3. [Q42440-1 ]
UniGenei At.48165.
At.48988.

3D structure databases

ProteinModelPortali Q42440.
SMRi Q42440. Positions 68-273.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q42440.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G07780.1 ; AT1G07780.1 ; AT1G07780 . [Q42440-1 ]
AT1G07780.2 ; AT1G07780.2 ; AT1G07780 . [Q42440-1 ]
AT1G07780.3 ; AT1G07780.3 ; AT1G07780 . [Q42440-1 ]
AT1G07780.5 ; AT1G07780.5 ; AT1G07780 . [Q42440-1 ]
GeneIDi 837292.
KEGGi ath:AT1G07780.

Organism-specific databases

TAIRi AT1G07780.

Phylogenomic databases

KOi K01817.
OMAi PDYAGFV.
PhylomeDBi Q42440.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00042 .
BioCyci ARA:AT1G07780-MONOMER.
ARA:GQT-133-MONOMER.
ARA:GQT-134-MONOMER.
ARA:GQT-135-MONOMER.

Gene expression databases

ExpressionAtlasi Q42440. baseline.
Genevestigatori Q42440.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00135. PRAI.
InterProi IPR013785. Aldolase_TIM.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF00697. PRAI. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis."
    Bender J., Fink G.R.
    Cell 83:725-734(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Wassilewskija.
  2. "Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes."
    Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.
    Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Arabidopsis PAI gene arrangements, cytosine methylation and expression."
    Melquist S., Luff B., Bender J.
    Genetics 153:401-413(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Columbia.
  4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  5. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Differential expression of triplicate phosphoribosylanthranilate isomerase isogenes in the tryptophan biosynthetic pathway of Arabidopsis thaliana (L.) Heynh."
    He Y., Li J.
    Planta 212:641-647(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
    Strain: cv. Wassilewskija.
  8. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
    Olinares P.D., Ponnala L., van Wijk K.J.
    Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPAI1_ARATH
AccessioniPrimary (citable) accession number: Q42440
Secondary accession number(s): Q3EDG7, Q7G9J9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3