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Q42440

- PAI1_ARATH

UniProt

Q42440 - PAI1_ARATH

Protein

N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic

Gene

PAI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. phosphoribosylanthranilate isomerase activity Source: TAIR

    GO - Biological processi

    1. tryptophan biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT1G07780-MONOMER.
    ARA:GQT-133-MONOMER.
    ARA:GQT-134-MONOMER.
    ARA:GQT-135-MONOMER.
    UniPathwayiUPA00035; UER00042.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (EC:5.3.1.24)
    Gene namesi
    Name:PAI1
    Synonyms:TRP6
    Ordered Locus Names:At1g07780
    ORF Names:F24B9.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G07780.

    Subcellular locationi

    Plastidchloroplast 2 Publications

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Bushy morphology, reduced fertility, blue fluorescence under UV light and resistance to the anthranilate analog 6-methylanthranilate.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232ChloroplastSequence AnalysisAdd
    BLAST
    Chaini33 – 275243N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplasticPRO_0000417453Add
    BLAST

    Proteomic databases

    PRIDEiQ42440.

    Expressioni

    Tissue specificityi

    Expressed in roots and shoots.1 Publication

    Inductioni

    By silver nitrate and UV irradiation.1 Publication

    Gene expression databases

    GenevestigatoriQ42440.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42440.
    SMRiQ42440. Positions 68-273.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TrpF family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    InParanoidiQ42440.
    KOiK01817.
    OMAiPDYAGFV.
    PhylomeDBiQ42440.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00135. PRAI.
    InterProiIPR013785. Aldolase_TIM.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00697. PRAI. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q42440-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN    50
    TENVAPKDDE RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR 100
    SISLSVAKDI SKVAREGGAK PVGVFVEDDE NTILRAADSS DLELVQLHGN 150
    GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE VPEEDCHLAD WILVDSATGG 200
    SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ PDGIDVSSGI 250
    CGTDGIQKDK SKISSFITAV RSVHY 275
    Length:275
    Mass (Da):29,633
    Last modified:November 1, 1996 - v1
    Checksum:i5F4417E94255F2C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34756 Genomic DNA. Translation: AAB03498.1.
    U18970 Genomic DNA. Translation: AAC49005.1.
    AF130878 Genomic DNA. Translation: AAD38141.1.
    AC007583 Genomic DNA. Translation: AAF75075.1.
    CP002684 Genomic DNA. Translation: AEE28179.1.
    CP002684 Genomic DNA. Translation: AEE28180.1.
    CP002684 Genomic DNA. Translation: AEE28181.1.
    CP002684 Genomic DNA. Translation: AEE28183.1.
    AY081274 mRNA. Translation: AAL91163.1.
    BT003351 mRNA. Translation: AAO29969.1.
    PIRiC86213.
    RefSeqiNP_001184931.1. NM_001198002.1. [Q42440-1]
    NP_172257.1. NM_100652.4. [Q42440-1]
    NP_849606.1. NM_179275.3. [Q42440-1]
    NP_973784.2. NM_202055.3. [Q42440-1]
    UniGeneiAt.48165.
    At.48988.

    Genome annotation databases

    EnsemblPlantsiAT1G07780.1; AT1G07780.1; AT1G07780. [Q42440-1]
    AT1G07780.2; AT1G07780.2; AT1G07780. [Q42440-1]
    AT1G07780.3; AT1G07780.3; AT1G07780. [Q42440-1]
    AT1G07780.5; AT1G07780.5; AT1G07780. [Q42440-1]
    GeneIDi837292.
    KEGGiath:AT1G07780.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34756 Genomic DNA. Translation: AAB03498.1 .
    U18970 Genomic DNA. Translation: AAC49005.1 .
    AF130878 Genomic DNA. Translation: AAD38141.1 .
    AC007583 Genomic DNA. Translation: AAF75075.1 .
    CP002684 Genomic DNA. Translation: AEE28179.1 .
    CP002684 Genomic DNA. Translation: AEE28180.1 .
    CP002684 Genomic DNA. Translation: AEE28181.1 .
    CP002684 Genomic DNA. Translation: AEE28183.1 .
    AY081274 mRNA. Translation: AAL91163.1 .
    BT003351 mRNA. Translation: AAO29969.1 .
    PIRi C86213.
    RefSeqi NP_001184931.1. NM_001198002.1. [Q42440-1 ]
    NP_172257.1. NM_100652.4. [Q42440-1 ]
    NP_849606.1. NM_179275.3. [Q42440-1 ]
    NP_973784.2. NM_202055.3. [Q42440-1 ]
    UniGenei At.48165.
    At.48988.

    3D structure databases

    ProteinModelPortali Q42440.
    SMRi Q42440. Positions 68-273.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q42440.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G07780.1 ; AT1G07780.1 ; AT1G07780 . [Q42440-1 ]
    AT1G07780.2 ; AT1G07780.2 ; AT1G07780 . [Q42440-1 ]
    AT1G07780.3 ; AT1G07780.3 ; AT1G07780 . [Q42440-1 ]
    AT1G07780.5 ; AT1G07780.5 ; AT1G07780 . [Q42440-1 ]
    GeneIDi 837292.
    KEGGi ath:AT1G07780.

    Organism-specific databases

    TAIRi AT1G07780.

    Phylogenomic databases

    InParanoidi Q42440.
    KOi K01817.
    OMAi PDYAGFV.
    PhylomeDBi Q42440.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00042 .
    BioCyci ARA:AT1G07780-MONOMER.
    ARA:GQT-133-MONOMER.
    ARA:GQT-134-MONOMER.
    ARA:GQT-135-MONOMER.

    Gene expression databases

    Genevestigatori Q42440.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00135. PRAI.
    InterProi IPR013785. Aldolase_TIM.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00697. PRAI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis."
      Bender J., Fink G.R.
      Cell 83:725-734(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Wassilewskija.
    2. "Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes."
      Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.
      Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    3. "Arabidopsis PAI gene arrangements, cytosine methylation and expression."
      Melquist S., Luff B., Bender J.
      Genetics 153:401-413(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. Columbia.
    4. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Differential expression of triplicate phosphoribosylanthranilate isomerase isogenes in the tryptophan biosynthetic pathway of Arabidopsis thaliana (L.) Heynh."
      He Y., Li J.
      Planta 212:641-647(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
      Strain: cv. Wassilewskija.
    8. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. "Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
      Olinares P.D., Ponnala L., van Wijk K.J.
      Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPAI1_ARATH
    AccessioniPrimary (citable) accession number: Q42440
    Secondary accession number(s): Q3EDG7, Q7G9J9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3