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Protein

N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic

Gene

PAI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase beta subunit 2, chloroplastic (ASB2), Anthranilate synthase beta subunit 1, chloroplastic (ASB1), Anthranilate synthase alpha subunit 1, chloroplastic (ASA1), Anthranilate synthase alpha subunit 2, chloroplastic (ASA2)
  2. Anthranilate phosphoribosyltransferase, chloroplastic (PAT1)
  3. N-(5'-phosphoribosyl)anthranilate isomerase 2, chloroplastic (PAI2), N-(5'-phosphoribosyl)anthranilate isomerase 3, chloroplastic (PAI3), N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (PAI1)
  4. Indole-3-glycerol phosphate synthase, chloroplastic (IGPS)
  5. Tryptophan synthase alpha chain (TRPA1), Tryptophan synthase alpha chain, chloroplastic (TSA1), Tryptophan synthase (AXX17_At5g53930), Tryptophan synthase (At5g54810), Tryptophan synthase (At5g28237), Tryptophan synthase (AXX17_At5g28490), Tryptophan synthase (AXX17_At4g31220), Tryptophan synthase, Tryptophan synthase beta chain 1, chloroplastic (TSB1), Tryptophan synthase (At4g27070), Tryptophan synthase (At5g28237), Tryptophan synthase beta chain 2, chloroplastic (TSB2), Tryptophan synthase (AXX17_At4g31220)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • phosphoribosylanthranilate isomerase activity Source: TAIR

GO - Biological processi

  • tryptophan biosynthetic process Source: TAIR

Keywordsi

Molecular functionIsomerase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciARA:AT1G07780-MONOMER.
ARA:GQT-135-MONOMER.
UniPathwayiUPA00035; UER00042.

Names & Taxonomyi

Protein namesi
Recommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic (EC:5.3.1.24)
Gene namesi
Name:PAI1
Synonyms:TRP6
Ordered Locus Names:At1g07780
ORF Names:F24B9.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G07780.
TAIRilocus:2026509. AT1G07780.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Bushy morphology, reduced fertility, blue fluorescence under UV light and resistance to the anthranilate analog 6-methylanthranilate.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32ChloroplastSequence analysisAdd BLAST32
ChainiPRO_000041745333 – 275N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplasticAdd BLAST243

Proteomic databases

PaxDbiQ42440.
PRIDEiQ42440.

Expressioni

Tissue specificityi

Expressed in roots and shoots.1 Publication

Inductioni

By silver nitrate and UV irradiation.1 Publication

Gene expression databases

ExpressionAtlasiQ42440. baseline and differential.
GenevisibleiQ42440. AT.

Interactioni

Protein-protein interaction databases

BioGridi22533. 1 interactor.
STRINGi3702.AT1G07780.1.

Structurei

3D structure databases

ProteinModelPortaliQ42440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpF family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG4202. Eukaryota.
COG0135. LUCA.
KOiK01817.
OMAiFYAKSPR.
OrthoDBiEOG09360HV1.
PhylomeDBiQ42440.

Family and domain databases

CDDicd00405. PRAI. 1 hit.
Gene3Di3.20.20.70. 2 hits.
HAMAPiMF_00135. PRAI. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR001240. PRAI.
IPR011060. RibuloseP-bd_barrel.
PfamiView protein in Pfam
PF00697. PRAI. 1 hit.
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42440-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN
60 70 80 90 100
TENVAPKDDE RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR
110 120 130 140 150
SISLSVAKDI SKVAREGGAK PVGVFVEDDE NTILRAADSS DLELVQLHGN
160 170 180 190 200
GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE VPEEDCHLAD WILVDSATGG
210 220 230 240 250
SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ PDGIDVSSGI
260 270
CGTDGIQKDK SKISSFITAV RSVHY
Length:275
Mass (Da):29,633
Last modified:November 1, 1996 - v1
Checksum:i5F4417E94255F2C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34756 Genomic DNA. Translation: AAB03498.1.
U18970 Genomic DNA. Translation: AAC49005.1.
AF130878 Genomic DNA. Translation: AAD38141.1.
AC007583 Genomic DNA. Translation: AAF75075.1.
CP002684 Genomic DNA. Translation: AEE28179.1.
CP002684 Genomic DNA. Translation: AEE28180.1.
CP002684 Genomic DNA. Translation: AEE28181.1.
CP002684 Genomic DNA. Translation: AEE28183.1.
CP002684 Genomic DNA. Translation: ANM60071.1.
CP002684 Genomic DNA. Translation: ANM60072.1.
CP002684 Genomic DNA. Translation: ANM60074.1.
CP002684 Genomic DNA. Translation: ANM60075.1.
AY081274 mRNA. Translation: AAL91163.1.
BT003351 mRNA. Translation: AAO29969.1.
PIRiC86213.
RefSeqiNP_001184931.1. NM_001198002.2. [Q42440-1]
NP_001322383.1. NM_001331736.1. [Q42440-1]
NP_001322384.1. NM_001331729.1. [Q42440-1]
NP_001322386.1. NM_001331731.1. [Q42440-1]
NP_001322387.1. NM_001331732.1. [Q42440-1]
NP_172257.1. NM_100652.5. [Q42440-1]
NP_849606.1. NM_179275.4. [Q42440-1]
NP_973784.2. NM_202055.4. [Q42440-1]
UniGeneiAt.48165.
At.48988.

Genome annotation databases

EnsemblPlantsiAT1G07780.1; AT1G07780.1; AT1G07780. [Q42440-1]
AT1G07780.10; AT1G07780.10; AT1G07780. [Q42440-1]
AT1G07780.12; AT1G07780.12; AT1G07780. [Q42440-1]
AT1G07780.13; AT1G07780.13; AT1G07780. [Q42440-1]
AT1G07780.2; AT1G07780.2; AT1G07780. [Q42440-1]
AT1G07780.3; AT1G07780.3; AT1G07780. [Q42440-1]
AT1G07780.5; AT1G07780.5; AT1G07780. [Q42440-1]
AT1G07780.9; AT1G07780.9; AT1G07780. [Q42440-1]
GeneIDi837292.
GrameneiAT1G07780.1; AT1G07780.1; AT1G07780.
AT1G07780.10; AT1G07780.10; AT1G07780.
AT1G07780.12; AT1G07780.12; AT1G07780.
AT1G07780.13; AT1G07780.13; AT1G07780.
AT1G07780.2; AT1G07780.2; AT1G07780.
AT1G07780.3; AT1G07780.3; AT1G07780.
AT1G07780.5; AT1G07780.5; AT1G07780.
AT1G07780.9; AT1G07780.9; AT1G07780.
KEGGiath:AT1G07780.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPAI1_ARATH
AccessioniPrimary (citable) accession number: Q42440
Secondary accession number(s): Q3EDG7, Q7G9J9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1996
Last modified: August 30, 2017
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families