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Q42440 (PAI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic

EC=5.3.1.24
Gene names
Name:PAI1
Synonyms:TRP6
Ordered Locus Names:At1g07780
ORF Names:F24B9.11
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5. HAMAP-Rule MF_00135

Subcellular location

Plastidchloroplast Ref.8 Ref.9.

Tissue specificity

Expressed in roots and shoots. Ref.7

Induction

By silver nitrate and UV irradiation. Ref.7

Disruption phenotype

Bushy morphology, reduced fertility, blue fluorescence under UV light and resistance to the anthranilate analog 6-methylanthranilate. Ref.7

Sequence similarities

Belongs to the TrpF family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Aromatic amino acid biosynthesis
Tryptophan biosynthesis
   Cellular componentChloroplast
Plastid
   Coding sequence diversityAlternative splicing
   DomainTransit peptide
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtryptophan biosynthetic process

Inferred from mutant phenotype Ref.1. Source: TAIR

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphoribosylanthranilate isomerase activity

Inferred from mutant phenotype Ref.2. Source: TAIR

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q42440-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3232Chloroplast Potential
Chain33 – 275243N-(5'-phosphoribosyl)anthranilate isomerase 1, chloroplastic HAMAP-Rule MF_00135
PRO_0000417453

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 5F4417E94255F2C6

FASTA27529,633
        10         20         30         40         50         60 
MSTGISTDLH VHFGALNFSK TYKSGLSNRT VSFSRVGYAQ NRKLSCSVSN TENVAPKDDE 

        70         80         90        100        110        120 
RGKDRPLVKM CGITSARDAA MAVEAGADFI GMIIWPHSKR SISLSVAKDI SKVAREGGAK 

       130        140        150        160        170        180 
PVGVFVEDDE NTILRAADSS DLELVQLHGN GSRAAFSRLV RKRRVIYVLN ANQDGKLLNE 

       190        200        210        220        230        240 
VPEEDCHLAD WILVDSATGG SGHGFNWAQF KLPSVRSRNG WLLAGGINPT NVSEALSILQ 

       250        260        270 
PDGIDVSSGI CGTDGIQKDK SKISSFITAV RSVHY 

« Hide

References

« Hide 'large scale' references
[1]"Epigenetic control of an endogenous gene family is revealed by a novel blue fluorescent mutant of Arabidopsis."
Bender J., Fink G.R.
Cell 83:725-734(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Wassilewskija.
[2]"Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes."
Li J., Zhao J., Rose A.B., Schmidt R., Last R.L.
Plant Cell 7:447-461(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[3]"Arabidopsis PAI gene arrangements, cytosine methylation and expression."
Melquist S., Luff B., Bender J.
Genetics 153:401-413(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Columbia.
[4]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Differential expression of triplicate phosphoribosylanthranilate isomerase isogenes in the tryptophan biosynthetic pathway of Arabidopsis thaliana (L.) Heynh."
He Y., Li J.
Planta 212:641-647(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
Strain: cv. Wassilewskija.
[8]"AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]"Megadalton complexes in the chloroplast stroma of Arabidopsis thaliana characterized by size exclusion chromatography, mass spectrometry, and hierarchical clustering."
Olinares P.D., Ponnala L., van Wijk K.J.
Mol. Cell. Proteomics 9:1594-1615(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U34756 Genomic DNA. Translation: AAB03498.1.
U18970 Genomic DNA. Translation: AAC49005.1.
AF130878 Genomic DNA. Translation: AAD38141.1.
AC007583 Genomic DNA. Translation: AAF75075.1.
CP002684 Genomic DNA. Translation: AEE28179.1.
CP002684 Genomic DNA. Translation: AEE28180.1.
CP002684 Genomic DNA. Translation: AEE28181.1.
CP002684 Genomic DNA. Translation: AEE28183.1.
AY081274 mRNA. Translation: AAL91163.1.
BT003351 mRNA. Translation: AAO29969.1.
PIRC86213.
RefSeqNP_001184931.1. NM_001198002.1. [Q42440-1]
NP_172257.1. NM_100652.4. [Q42440-1]
NP_849606.1. NM_179275.3. [Q42440-1]
NP_973784.2. NM_202055.3. [Q42440-1]
UniGeneAt.48165.
At.48988.

3D structure databases

ProteinModelPortalQ42440.
SMRQ42440. Positions 68-273.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ42440.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G07780.1; AT1G07780.1; AT1G07780. [Q42440-1]
AT1G07780.2; AT1G07780.2; AT1G07780. [Q42440-1]
AT1G07780.3; AT1G07780.3; AT1G07780. [Q42440-1]
AT1G07780.5; AT1G07780.5; AT1G07780. [Q42440-1]
GeneID837292.
KEGGath:AT1G07780.

Organism-specific databases

TAIRAT1G07780.

Phylogenomic databases

InParanoidQ42440.
KOK01817.
OMAPDYAGFV.
PhylomeDBQ42440.

Enzyme and pathway databases

BioCycARA:AT1G07780-MONOMER.
ARA:GQT-133-MONOMER.
ARA:GQT-134-MONOMER.
ARA:GQT-135-MONOMER.
UniPathwayUPA00035; UER00042.

Gene expression databases

GenevestigatorQ42440.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamPF00697. PRAI. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
ProtoNetSearch...

Entry information

Entry namePAI1_ARATH
AccessionPrimary (citable) accession number: Q42440
Secondary accession number(s): Q3EDG7, Q7G9J9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names