Q42434 (BIP_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Luminal-binding protein
Short name=BiP

Alternative name(s):
78 kDa glucose-regulated protein homolog
Short name=GRP-78

Gene names

Name:

HSC70

Organism

Spinacia oleracea (Spinach)

Taxonomic identifier

3562 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
Subcellular location	Endoplasmic reticulum lumen.
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Signal
Ligand	ATP-binding Nucleotide-binding
PTM	Glycoprotein
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 668	640	Luminal-binding protein		PRO_0000013593
Regions
	Motif	665 – 668	4	Prevents secretion from ER Potential
Amino acid modifications
	Glycosylation	617	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q42434 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 68043D93B56196A8	FASTA	668	73,592
	10 20 30 40 50 60 MAVAWKSRAS SIAFGIVLLG SLFAFVSAKD EAPKLGTVIG IDLGTTYSCV GVYKDGKVEI 70 80 90 100 110 120 IANDQGNRIT PSWVAFTNDE RLIGEAAKNQ AAANPERTIF DVKRLIGRKF EDKEVQKDMK 130 140 150 160 170 180 LVPYKIVNRD GKPYIQVKVQ EGETKVFSPE EISAMILTKM KETAETFLGK KIKDAVVTVP 190 200 210 220 230 240 AYFNDAQRQA TKDAGVIAGL NVARIINEPT AAAIAYGLDK RGGEKNILVF DLGGGTFDVS 250 260 270 280 290 300 VLTIDNGVFE VLATNGDTHL GGEDFDQRLM EYFIKLIKKK HTKDISKDNR ALGKLRRECE 310 320 330 340 350 360 RAKRALSSQH QVRVEIESLF DGVDFSEPLT RARFEELNND LFRKTMGPVK KAMDDAGLEK 370 380 390 400 410 420 NQIDEIVLVG GSTRIPKVQQ LLKEFFNGKE PSKGVNPDEA VAFGAAVQGS ILSGEGGEET 430 440 450 460 470 480 KEILLLDVAP LTLGIETVGG VMTKLIPRNT VIPTKKSQVF TTYQDQQTTV TIQVFEGERS 490 500 510 520 530 540 LTKDCRLLGK FDLTGIAPAP RGTPQIEVTF EVDANGILNV KAEDKASGKS EKITITNDKG 550 560 570 580 590 600 RLSQEEIERM VREAEEFAEE DKKVKEKIDA RNSLETYIYN MKNQISDADK LADKLESDEK 610 620 630 640 650 660 EKIEGAVKEA LEWLDDNQSA EKEDYDEKLK EVEAVCNPII TAVYQRSGGP SGESGADSED SEEGHDEL

References

[1]	"A cDNA encoding the endoplasmic reticulum-luminal heat-shock protein from spinach (Spinacia oleracea L.)." Anderson J.V., Neven L.G., Li Q.B., Haskell D.W., Guy C.L. Plant Physiol. 104:303-304(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Bloomsdale. Tissue: Leaf.
[2]	"Structural organization of the spinach endoplasmic reticulum-luminal 70-kilodalton heat-shock cognate gene and expression of 70-kilodalton heat-shock genes during cold acclimation." Anderson J.V., Li Q.B., Haskell D.W., Guy C.L. Plant Physiol. 104:1359-1370(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Bloomsdale. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23551 mRNA. Translation: AAA21808.1. L23552 Genomic DNA. Translation: AAA21806.1.
3D structure databases
ProteinModelPortal	Q42434.
ModBase	Search...
Proteomic databases
PRIDE	Q42434.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR018181. Heat_shock_70_CS. IPR013126. Hsp_70_fam. [Graphical view]
Pfam	PF00012. HSP70. 1 hit. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
PROSITE	PS00014. ER_TARGET. 1 hit. PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIP_SPIOL

Accession

Primary (citable) accession number: Q42434

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program