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Protein

Thioredoxin H3

Gene

TRX3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-disulfide oxidoreductase that possesses disulfide reductase and insulin disulfide bonds reducing activities. Heat shock causes oligomerization and formation of high molecular weight (HMW) complexes with concomitant functional switching from a disulfide reductase to chaperone.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei39NucleophileSequence analysis1
Active sitei42NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: GO_Central
  • cellular response to oxidative stress Source: GO_Central
  • defense response to fungus Source: TAIR
  • glycerol ether metabolic process Source: InterPro
  • heat acclimation Source: UniProtKB
  • protein folding Source: UniProtKB
  • protein oligomerization Source: UniProtKB
  • response to cytokinin Source: TAIR
  • response to heat Source: UniProtKB
  • response to microbial phytotoxin Source: TAIR
  • sulfate assimilation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Electron transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Thioredoxin H3
Short name:
AtTrxh3
Alternative name(s):
Thioredoxin 3
Short name:
AtTRX3
Gene namesi
Name:TRX3
Ordered Locus Names:At5g42980
ORF Names:MBD2.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G42980.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • cytosol Source: UniProtKB
  • Golgi apparatus Source: TAIR
  • membrane Source: TAIR
  • mitochondrion Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

High sensitivity to heat shock.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39C → S: Loss of disulfide reductase activity. 1 Publication1
Mutagenesisi42C → S: Loss of disulfide reductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001200482 – 118Thioredoxin H3Add BLAST117

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Disulfide bondi39 ↔ 42Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiQ42403.
PRIDEiQ42403.

PTM databases

SwissPalmiQ42403.

Expressioni

Gene expression databases

GenevisibleiQ42403. AT.

Interactioni

Subunit structurei

Interacts with FBA6.1 Publication

Protein-protein interaction databases

BioGridi19563. 19 interactors.
DIPiDIP-32720N.
IntActiQ42403. 50 interactors.
STRINGi3702.AT5G42980.1.

Structurei

3D structure databases

ProteinModelPortaliQ42403.
SMRiQ42403.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 113ThioredoxinPROSITE-ProRule annotationAdd BLAST112

Sequence similaritiesi

Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000292977.
InParanoidiQ42403.
KOiK03671.
OMAiVVFFKVD.
OrthoDBiEOG09360XJ5.
PhylomeDBiQ42403.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42403-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAEGEVIAC HTVEDWTEKL KAANESKKLI VIDFTATWCP PCRFIAPVFA
60 70 80 90 100
DLAKKHLDVV FFKVDVDELN TVAEEFKVQA MPTFIFMKEG EIKETVVGAA
110
KEEIIANLEK HKTVVAAA
Length:118
Mass (Da):13,109
Last modified:November 1, 1996 - v1
Checksum:i7AFCE291C3EB8FE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35474 mRNA. Translation: CAA84611.1.
U35640 Genomic DNA. Translation: AAC49351.1.
AB008264 Genomic DNA. Translation: BAB09200.1.
CP002688 Genomic DNA. Translation: AED94897.1.
AY059870 mRNA. Translation: AAL24352.1.
AY065098 mRNA. Translation: AAL38274.1.
AY093318 mRNA. Translation: AAM13317.1.
AY114566 mRNA. Translation: AAM47885.1.
AY085117 mRNA. Translation: AAM61671.1.
Z35335 mRNA. Translation: CAA84560.1.
PIRiS58118.
RefSeqiNP_199112.1. NM_123664.4.
UniGeneiAt.24175.

Genome annotation databases

EnsemblPlantsiAT5G42980.1; AT5G42980.1; AT5G42980.
GeneIDi834313.
GrameneiAT5G42980.1; AT5G42980.1; AT5G42980.
KEGGiath:AT5G42980.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z35474 mRNA. Translation: CAA84611.1.
U35640 Genomic DNA. Translation: AAC49351.1.
AB008264 Genomic DNA. Translation: BAB09200.1.
CP002688 Genomic DNA. Translation: AED94897.1.
AY059870 mRNA. Translation: AAL24352.1.
AY065098 mRNA. Translation: AAL38274.1.
AY093318 mRNA. Translation: AAM13317.1.
AY114566 mRNA. Translation: AAM47885.1.
AY085117 mRNA. Translation: AAM61671.1.
Z35335 mRNA. Translation: CAA84560.1.
PIRiS58118.
RefSeqiNP_199112.1. NM_123664.4.
UniGeneiAt.24175.

3D structure databases

ProteinModelPortaliQ42403.
SMRiQ42403.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19563. 19 interactors.
DIPiDIP-32720N.
IntActiQ42403. 50 interactors.
STRINGi3702.AT5G42980.1.

PTM databases

SwissPalmiQ42403.

Proteomic databases

PaxDbiQ42403.
PRIDEiQ42403.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G42980.1; AT5G42980.1; AT5G42980.
GeneIDi834313.
GrameneiAT5G42980.1; AT5G42980.1; AT5G42980.
KEGGiath:AT5G42980.

Organism-specific databases

TAIRiAT5G42980.

Phylogenomic databases

eggNOGiKOG0907. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000292977.
InParanoidiQ42403.
KOiK03671.
OMAiVVFFKVD.
OrthoDBiEOG09360XJ5.
PhylomeDBiQ42403.

Miscellaneous databases

PROiQ42403.

Gene expression databases

GenevisibleiQ42403. AT.

Family and domain databases

Gene3Di3.40.30.10. 1 hit.
InterProiIPR005746. Thioredoxin.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PANTHERiPTHR10438. PTHR10438. 1 hit.
PfamiPF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRXH3_ARATH
AccessioniPrimary (citable) accession number: Q42403
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

The active site contains a CPPC motif wich differs from the conserved CGPC motif.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.