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Protein

Protein pleiotropic regulatory locus 1

Gene

PRL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Pleiotropic regulator of glucose, stress and hormone responses. Also regulates cytochrome P450 CYP90A1/CPD. Coordinates the expression of hormone- and stress-related genes and genes related to cell wall modification and growth, leading to altered sugar-dependent growth and developmental responses. Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity. By suppressing the expression of several (1)O2-responsive genes, PRL1 seems to play a major role in modulating responses of plants to environmental changes by interconnecting (1)O2-mediated retrograde signaling with other signaling pathways. Acts as negative regulator of SNF1-related protein kinases AKIN10 and AKIN11 via the inhibition of their interaction with SKP1/ASK1. Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex, PRL1 may function as the substrate recognition module within this complex, leading to the AKIN10 degradation.9 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • cotyledon development Source: TAIR
  • defense response signaling pathway, resistance gene-dependent Source: TAIR
  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • fruit development Source: TAIR
  • hormone-mediated signaling pathway Source: TAIR
  • innate immune response Source: UniProtKB-KW
  • leaf development Source: TAIR
  • mRNA splicing, via spliceosome Source: GO_Central
  • negative regulation of transcription, DNA-templated Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • proteolysis Source: TAIR
  • response to glucose Source: TAIR
  • root development Source: TAIR
  • sugar mediated signaling pathway Source: TAIR

Keywordsi

Biological processImmunity, Innate immunity, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-72163 mRNA Splicing - Major Pathway
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Protein pleiotropic regulatory locus 1
Short name:
Protein PRL1
Alternative name(s):
MOS4-associated complex protein 2
Short name:
MAC protein 2
Gene namesi
Name:PRL1
Synonyms:MAC2
Ordered Locus Names:At4g15900
ORF Names:dl3990w, FCAALL.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G15900
TAIRilocus:2005539 AT4G15900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to glucose and sucrose. Enhanced sensitivity of plants to stress and to growth hormones including cytokinin, ethylene, abscisic acid, and auxin. Accumulation of sugars and starch in leaves, and root elongation. Cell elongation defects. Enhanced susceptibility to virulent and avirulent pathogens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000511571 – 486Protein pleiotropic regulatory locus 1Add BLAST486

Proteomic databases

PaxDbiQ42384

PTM databases

iPTMnetiQ42384

Expressioni

Gene expression databases

ExpressionAtlasiQ42384 baseline and differential
GenevisibleiQ42384 AT

Interactioni

Subunit structurei

Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 (PubMed:17575050, PubMed:19629177). Interacts with CDC5 (PubMed:17575050). Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex. Interacts with DDB1A through its DWD motif (PubMed:18223036). Interacts with AKIN10, AKIN11 and PIPC (PubMed:10220464). Interacts with KAP2 (PubMed:9765207).5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi12566, 18 interactors
IntActiQ42384, 10 interactors
STRINGi3702.AT4G15900.1

Structurei

3D structure databases

ProteinModelPortaliQ42384
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati174 – 204WD 1Add BLAST31
Repeati216 – 246WD 2Add BLAST31
Repeati258 – 288WD 3Add BLAST31
Repeati300 – 330WD 4Add BLAST31
Repeati342 – 371WD 5Add BLAST30
Repeati384 – 413WD 6Add BLAST30
Repeati433 – 463WD 7Add BLAST31

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi275 – 290DWD box 1Add BLAST16
Motifi317 – 332DWD box 2Add BLAST16

Domaini

The DWD box is required for interaction with DDB1A.1 Publication

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0285 Eukaryota
ENOG410XQV9 LUCA
HOGENOMiHOG000207595
InParanoidiQ42384
KOiK12862
OMAiFAMCFDQ
OrthoDBiEOG09360800
PhylomeDBiQ42384

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR019775 WD40_repeat_CS
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 5 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS00678 WD_REPEATS_1, 2 hits
PS50082 WD_REPEATS_2, 5 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q42384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPTTEIEP IEAQSLKKLS LKSLKRSLEL FSPVHGQFPP PDPEAKQIRL
60 70 80 90 100
SHKMKVAFGG VEPVVSQPPR QPDRINEQPG PSNALSLAAP EGSKSTQKGA
110 120 130 140 150
TESAIVVGPT LLRPILPKGL NYTGSSGKST TIIPANVSSY QRNLSTAALM
160 170 180 190 200
ERIPSRWPRP EWHAPWKNYR VIQGHLGWVR SVAFDPSNEW FCTGSADRTI
210 220 230 240 250
KIWDVATGVL KLTLTGHIEQ VRGLAVSNRH TYMFSAGDDK QVKCWDLEQN
260 270 280 290 300
KVIRSYHGHL SGVYCLALHP TLDVLLTGGR DSVCRVWDIR TKMQIFALSG
310 320 330 340 350
HDNTVCSVFT RPTDPQVVTG SHDTTIKFWD LRYGKTMSTL THHKKSVRAM
360 370 380 390 400
TLHPKENAFA SASADNTKKF SLPKGEFCHN MLSQQKTIIN AMAVNEDGVM
410 420 430 440 450
VTGGDNGSIW FWDWKSGHSF QQSETIVQPG SLESEAGIYA ACYDNTGSRL
460 470 480
VTCEADKTIK MWKEDENATP ETHPINFKPP KEIRRF
Length:486
Mass (Da):54,009
Last modified:November 1, 1996 - v1
Checksum:i604D3E6FCDA8A998
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82824 Genomic DNA Translation: CAA58031.1
X82825 mRNA Translation: CAA58032.1
Z97339 Genomic DNA Translation: CAB10369.1
AL161542 Genomic DNA Translation: CAB78632.1
CP002687 Genomic DNA Translation: AEE83664.1
PIRiS49820
RefSeqiNP_193325.1, NM_117682.3
UniGeneiAt.103

Genome annotation databases

EnsemblPlantsiAT4G15900.1; AT4G15900.1; AT4G15900
GeneIDi827272
GrameneiAT4G15900.1; AT4G15900.1; AT4G15900
KEGGiath:AT4G15900

Similar proteinsi

Entry informationi

Entry nameiPRL1_ARATH
AccessioniPrimary (citable) accession number: Q42384
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health