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Protein

Protein pleiotropic regulatory locus 1

Gene

PRL1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pleiotropic regulator of glucose, stress and hormone responses. Also regulates cytochrome P450 CYP90A1/CPD. Coordinates the expression of hormone- and stress-related genes and genes related to cell wall modification and growth, leading to altered sugar-dependent growth and developmental responses. Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity. By suppressing the expression of several (1)O(2)-responsive genes, PRL1 seems to play a major role in modulating responses of plants to environmental changes by interconnecting (1)O(2)-mediated retrograde signaling with other signaling pathways. Acts as negative regulator of SNF1-related protein kinases AKIN10 and AKIN11 via the inhibition of their interaction with SKP1/ASK1. Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex, PRL1 may function as the substrate recognition module within this complex, leading to the AKIN10 degradation.9 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • cotyledon development Source: TAIR
  • defense response signaling pathway, resistance gene-dependent Source: TAIR
  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • defense response to bacterium Source: TAIR
  • defense response to fungus Source: TAIR
  • fruit development Source: TAIR
  • hormone-mediated signaling pathway Source: TAIR
  • innate immune response Source: UniProtKB-KW
  • leaf development Source: TAIR
  • mRNA splicing, via spliceosome Source: GO_Central
  • negative regulation of transcription, DNA-templated Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • proteolysis Source: TAIR
  • response to glucose Source: TAIR
  • root development Source: TAIR
  • sugar mediated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein pleiotropic regulatory locus 1
Short name:
Protein PRL1
Alternative name(s):
MOS4-associated complex protein 2
Short name:
MAC protein 2
Gene namesi
Name:PRL1
Synonyms:MAC2
Ordered Locus Names:At4g15900
ORF Names:dl3990w, FCAALL.40
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G15900.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: GO_Central
  • Cul4-RING E3 ubiquitin ligase complex Source: TAIR
  • nucleus Source: TAIR
  • precatalytic spliceosome Source: GO_Central
  • Prp19 complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Hypersensitivity to glucose and sucrose. Enhanced sensitivity of plants to stress and to growth hormones including cytokinin, ethylene, abscisic acid, and auxin. Accumulation of sugars and starch in leaves, and root elongation. Cell elongation defects. Enhanced susceptibility to virulent and avirulent pathogens.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Protein pleiotropic regulatory locus 1PRO_0000051157Add
BLAST

Proteomic databases

PaxDbiQ42384.
PRIDEiQ42384.

PTM databases

iPTMnetiQ42384.

Expressioni

Gene expression databases

ExpressionAtlasiQ42384. baseline and differential.
GenevisibleiQ42384. AT.

Interactioni

Subunit structurei

Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 (PubMed:17575050, PubMed:19629177). Interacts with CDC5 (PubMed:17575050). Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex. Interacts with DDB1A through its DWD motif (PubMed:18223036). Interacts with AKIN10, AKIN11 and PIPC (PubMed:10220464). Interacts with KAP2 (PubMed:9765207).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CDC5P929482EBI-1382964,EBI-1382948
IMPA3O042943EBI-1382964,EBI-1644689
KIN10Q389973EBI-1382964,EBI-2107143
KIN11P929582EBI-1382964,EBI-307202

Protein-protein interaction databases

BioGridi12566. 16 interactions.
IntActiQ42384. 10 interactions.
MINTiMINT-8070047.
STRINGi3702.AT4G15900.1.

Structurei

3D structure databases

ProteinModelPortaliQ42384.
SMRiQ42384. Positions 127-465.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati174 – 20431WD 1Add
BLAST
Repeati216 – 24631WD 2Add
BLAST
Repeati258 – 28831WD 3Add
BLAST
Repeati300 – 33031WD 4Add
BLAST
Repeati342 – 37130WD 5Add
BLAST
Repeati384 – 41330WD 6Add
BLAST
Repeati433 – 46331WD 7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi275 – 29016DWD box 1Add
BLAST
Motifi317 – 33216DWD box 2Add
BLAST

Domaini

The DWD box is required for interaction with DDB1A.1 Publication

Sequence similaritiesi

Belongs to the WD repeat PRL1/PRL2 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0285. Eukaryota.
ENOG410XQV9. LUCA.
HOGENOMiHOG000207595.
InParanoidiQ42384.
KOiK12862.
OMAiFMHNMLS.
OrthoDBiEOG09360800.
PhylomeDBiQ42384.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAPTTEIEP IEAQSLKKLS LKSLKRSLEL FSPVHGQFPP PDPEAKQIRL
60 70 80 90 100
SHKMKVAFGG VEPVVSQPPR QPDRINEQPG PSNALSLAAP EGSKSTQKGA
110 120 130 140 150
TESAIVVGPT LLRPILPKGL NYTGSSGKST TIIPANVSSY QRNLSTAALM
160 170 180 190 200
ERIPSRWPRP EWHAPWKNYR VIQGHLGWVR SVAFDPSNEW FCTGSADRTI
210 220 230 240 250
KIWDVATGVL KLTLTGHIEQ VRGLAVSNRH TYMFSAGDDK QVKCWDLEQN
260 270 280 290 300
KVIRSYHGHL SGVYCLALHP TLDVLLTGGR DSVCRVWDIR TKMQIFALSG
310 320 330 340 350
HDNTVCSVFT RPTDPQVVTG SHDTTIKFWD LRYGKTMSTL THHKKSVRAM
360 370 380 390 400
TLHPKENAFA SASADNTKKF SLPKGEFCHN MLSQQKTIIN AMAVNEDGVM
410 420 430 440 450
VTGGDNGSIW FWDWKSGHSF QQSETIVQPG SLESEAGIYA ACYDNTGSRL
460 470 480
VTCEADKTIK MWKEDENATP ETHPINFKPP KEIRRF
Length:486
Mass (Da):54,009
Last modified:November 1, 1996 - v1
Checksum:i604D3E6FCDA8A998
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82824 Genomic DNA. Translation: CAA58031.1.
X82825 mRNA. Translation: CAA58032.1.
Z97339 Genomic DNA. Translation: CAB10369.1.
AL161542 Genomic DNA. Translation: CAB78632.1.
CP002687 Genomic DNA. Translation: AEE83664.1.
PIRiS49820.
RefSeqiNP_193325.1. NM_117682.2.
UniGeneiAt.103.

Genome annotation databases

EnsemblPlantsiAT4G15900.1; AT4G15900.1; AT4G15900.
GeneIDi827272.
GrameneiAT4G15900.1; AT4G15900.1; AT4G15900.
KEGGiath:AT4G15900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82824 Genomic DNA. Translation: CAA58031.1.
X82825 mRNA. Translation: CAA58032.1.
Z97339 Genomic DNA. Translation: CAB10369.1.
AL161542 Genomic DNA. Translation: CAB78632.1.
CP002687 Genomic DNA. Translation: AEE83664.1.
PIRiS49820.
RefSeqiNP_193325.1. NM_117682.2.
UniGeneiAt.103.

3D structure databases

ProteinModelPortaliQ42384.
SMRiQ42384. Positions 127-465.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12566. 16 interactions.
IntActiQ42384. 10 interactions.
MINTiMINT-8070047.
STRINGi3702.AT4G15900.1.

PTM databases

iPTMnetiQ42384.

Proteomic databases

PaxDbiQ42384.
PRIDEiQ42384.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G15900.1; AT4G15900.1; AT4G15900.
GeneIDi827272.
GrameneiAT4G15900.1; AT4G15900.1; AT4G15900.
KEGGiath:AT4G15900.

Organism-specific databases

TAIRiAT4G15900.

Phylogenomic databases

eggNOGiKOG0285. Eukaryota.
ENOG410XQV9. LUCA.
HOGENOMiHOG000207595.
InParanoidiQ42384.
KOiK12862.
OMAiFMHNMLS.
OrthoDBiEOG09360800.
PhylomeDBiQ42384.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

PROiQ42384.

Gene expression databases

ExpressionAtlasiQ42384. baseline and differential.
GenevisibleiQ42384. AT.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 5 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 2 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRL1_ARATH
AccessioniPrimary (citable) accession number: Q42384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.