Reviewed, UniProtKB/Swiss-Prot Q42342 (CYB51_ARATH)

Names and origin

Protein names

Recommended name:
    Cytochrome b5 isoform 1

Gene names

Ordered Locus Names:	At5g53560
ORF Names:	MNC6.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR endoplasmic reticulum membrane Ref.1 Non-traceable author statement. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay. Source: TAIR vacuole Inferred from direct assay. Source: TAIR
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 134	134	Cytochrome b5 isoform 1		PRO_0000166020
Regions
	Transmembrane	107 – 127	21	Potential
	Domain	5 – 81	77	Cytochrome b5 heme-binding
Sites
	Metal binding	40	1	Iron (heme axial ligand) By similarity
	Metal binding	64	1	Iron (heme axial ligand) By similarity
Experimental info
	Sequence conflict	1 – 3	3	MSS → ARA in CAA23377. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q42342-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 9CC01C60F7C873FD	FASTA	134	15,084
	10 20 30 40 50 60 MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS TGKDATNDFE 70 80 90 100 110 120 DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN QDKTPEFIIK ILQFLVPILI 130 LGLALVVRHY TKKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis." Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D. Plant Physiol. 119:353-361(1999) [PubMed: 9880378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB007801 mRNA. Translation: BAA74839.1. AB015476 Genomic DNA. Translation: BAB09732.1. AY080878 mRNA. Translation: AAL87348.1. AY114045 mRNA. Translation: AAM45093.1. AY087070 mRNA. Translation: AAM64631.1. F20001 mRNA. Translation: CAA23377.1.
IPI	IPI00537177.
PIR	T52469.
RefSeq	NP_200168.1.
UniGene	At.20842
3D structure databases
HSSP	HSSP built from PDB template 1EHB based on UniProtKB P00171.
ModBase	Search...
Proteomic databases
PRIDE	Q42342.
Genome annotation databases
GeneID	835438.
GenomeReviews	Gene locus AT5G53560 in contig BA000015_GR.
KEGG	ath:AT5G53560.
NMPDR	fig|3702.1.peg.27252.
Organism-specific databases
TAIR	At5g53560.
Phylogenomic databases
OMA	Q42342. DVTPFMD.
Gene expression databases
ArrayExpress	Q42342.
GermOnline	AT5G53560. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB51_ARATH

Accession

Primary (citable) accession number: Q42342
Secondary accession number(s): Q9SB05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)