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Q42342 (CYB51_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cytochrome b5 isoform 1
Gene names
Ordered Locus Names:At5g53560
ORF Names:MNC6.10
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity.

Post-translational modification

Ubiquitinated. Ref.7

Sequence similarities

Belongs to the cytochrome b5 family.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 134134Cytochrome b5 isoform 1
PRO_0000166020

Regions

Transmembrane107 – 12721Helical; Potential
Domain5 – 8177Cytochrome b5 heme-binding

Sites

Metal binding401Iron (heme axial ligand) By similarity
Metal binding641Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict1 – 33MSS → ARA in CAA23377. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q42342 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 9CC01C60F7C873FD

FASTA13415,084
        10         20         30         40         50         60 
MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS TGKDATNDFE 

        70         80         90        100        110        120 
DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN QDKTPEFIIK ILQFLVPILI 

       130 
LGLALVVRHY TKKD 

« Hide

References

« Hide 'large scale' references
[1]"Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis."
Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D.
Plant Physiol. 119:353-361(1999) [PubMed: 9880378] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113.
Strain: cv. Columbia.
[7]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007801 mRNA. Translation: BAA74839.1.
AB015476 Genomic DNA. Translation: BAB09732.1.
CP002688 Genomic DNA. Translation: AED96377.1.
AY080878 mRNA. Translation: AAL87348.1.
AY114045 mRNA. Translation: AAM45093.1.
AY087070 mRNA. Translation: AAM64631.1.
F20001 mRNA. Translation: CAA23377.1.
IPIIPI00537177.
PIRT52469.
RefSeqNP_200168.1. NM_124736.3.
UniGeneAt.20842.

3D structure databases

ProteinModelPortalQ42342.
SMRQ42342. Positions 2-84.
ModBaseSearch...

Protein-protein interaction databases

IntActQ42342. 9 interactions.
STRINGQ42342.

Proteomic databases

PRIDEQ42342.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G53560.1; AT5G53560.1; AT5G53560.
GeneID835438.
GenomeReviewsGene locus AT5G53560 in contig BA000015_GR.
KEGGath:AT5G53560.
NMPDRfig|3702.1.peg.27252.

Organism-specific databases

TAIRAt5g53560.

Phylogenomic databases

GeneTreeEPGT00050000011061.
HOGENOMHBG629516.
InParanoidQ42342.
OMADTARDMM.
PhylomeDBQ42342.
ProtClustDBCLSN2686546.

Gene expression databases

ArrayExpressQ42342.
GenevestigatorQ42342.
GermOnlineAT5G53560. Arabidopsis thaliana.

Family and domain databases

InterProIPR001199. Cyt_B5.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
Gene3DG3DSA:3.10.120.10. Cyt_B5. 1 hit.
PfamPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. Cyt_B5. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYB51_ARATH
AccessionPrimary (citable) accession number: Q42342
Secondary accession number(s): Q9SB05
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: December 14, 2011
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families