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Q42342

- CYB5A_ARATH

UniProt

Q42342 - CYB5A_ARATH

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Protein
Cytochrome b5 isoform A
Gene
CYTB5-A, B5-A, At5g53560, MNC6.10
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases, including fatty acid desaturases.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Iron (heme axial ligand) By similarity
Metal bindingi64 – 641Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 isoform A
Alternative name(s):
Cytochrome b5 isoform 1
Gene namesi
Name:CYTB5-A
Synonyms:B5-A
Ordered Locus Names:At5g53560
ORF Names:MNC6.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G53560.

Subcellular locationi

Endoplasmic reticulum membrane; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei107 – 12721Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. endoplasmic reticulum membrane Source: TAIR
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: TAIR
  6. vacuolar membrane Source: TAIR
  7. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 134134Cytochrome b5 isoform A
PRO_0000166020Add
BLAST

Post-translational modificationi

Ubiquitinated.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ42342.
PRIDEiQ42342.

Expressioni

Tissue specificityi

Expressed in roots, stems, leaves, flowers and siliques.1 Publication

Gene expression databases

GenevestigatoriQ42342.

Interactioni

Subunit structurei

Interacts with CER1, FAH1 and FAH2.2 Publications

Protein-protein interaction databases

BioGridi20682. 11 interactions.
IntActiQ42342. 9 interactions.
STRINGi3702.AT5G53560.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ42342.
SMRiQ42342. Positions 10-85.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 8177Cytochrome b5 heme-binding
Add
BLAST

Sequence similaritiesi

Belongs to the cytochrome b5 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5274.
HOGENOMiHOG000039853.
InParanoidiQ42342.
OMAiYTISTTE.
PhylomeDBiQ42342.

Family and domain databases

Gene3Di3.10.120.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42342-1 [UniParc]FASTAAdd to Basket

« Hide

MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS    50
TGKDATNDFE DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN 100
QDKTPEFIIK ILQFLVPILI LGLALVVRHY TKKD 134
Length:134
Mass (Da):15,084
Last modified:January 11, 2001 - v2
Checksum:i9CC01C60F7C873FD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 33MSS → ARA in CAA23377. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007801 mRNA. Translation: BAA74839.1.
AB015476 Genomic DNA. Translation: BAB09732.1.
CP002688 Genomic DNA. Translation: AED96377.1.
AY080878 mRNA. Translation: AAL87348.1.
AY114045 mRNA. Translation: AAM45093.1.
AY087070 mRNA. Translation: AAM64631.1.
F20001 mRNA. Translation: CAA23377.1.
PIRiT52469.
RefSeqiNP_200168.1. NM_124736.3.
UniGeneiAt.20842.

Genome annotation databases

EnsemblPlantsiAT5G53560.1; AT5G53560.1; AT5G53560.
GeneIDi835438.
KEGGiath:AT5G53560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB007801 mRNA. Translation: BAA74839.1 .
AB015476 Genomic DNA. Translation: BAB09732.1 .
CP002688 Genomic DNA. Translation: AED96377.1 .
AY080878 mRNA. Translation: AAL87348.1 .
AY114045 mRNA. Translation: AAM45093.1 .
AY087070 mRNA. Translation: AAM64631.1 .
F20001 mRNA. Translation: CAA23377.1 .
PIRi T52469.
RefSeqi NP_200168.1. NM_124736.3.
UniGenei At.20842.

3D structure databases

ProteinModelPortali Q42342.
SMRi Q42342. Positions 10-85.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 20682. 11 interactions.
IntActi Q42342. 9 interactions.
STRINGi 3702.AT5G53560.1-P.

Proteomic databases

PaxDbi Q42342.
PRIDEi Q42342.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G53560.1 ; AT5G53560.1 ; AT5G53560 .
GeneIDi 835438.
KEGGi ath:AT5G53560.

Organism-specific databases

TAIRi AT5G53560.

Phylogenomic databases

eggNOGi COG5274.
HOGENOMi HOG000039853.
InParanoidi Q42342.
OMAi YTISTTE.
PhylomeDBi Q42342.

Miscellaneous databases

PROi Q42342.

Gene expression databases

Genevestigatori Q42342.

Family and domain databases

Gene3Di 3.10.120.10. 1 hit.
InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view ]
Pfami PF00173. Cyt-b5. 1 hit.
[Graphical view ]
PRINTSi PR00363. CYTOCHROMEB5.
SUPFAMi SSF55856. SSF55856. 1 hit.
PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis."
    Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D.
    Plant Physiol. 119:353-361(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
    Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113.
    Strain: cv. Columbia.
  7. "Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)."
    Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M.
    Plant J. 58:122-134(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FAH1 AND FAH2.
  8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
    Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
    Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
  9. "Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex."
    Bernard A., Domergue F., Pascal S., Jetter R., Renne C., Faure J.D., Haslam R.P., Napier J.A., Lessire R., Joubes J.
    Plant Cell 24:3106-3118(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CER1, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  10. "Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation."
    Kumar R., Tran L.S., Neelakandan A.K., Nguyen H.T.
    PLoS ONE 7:E31370-E31370(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiCYB5A_ARATH
AccessioniPrimary (citable) accession number: Q42342
Secondary accession number(s): Q9SB05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: May 14, 2014
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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