Q42342 (CYB51_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 104.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Cytochrome b5 isoform 1 | ||||
| Gene names |
| ||||
| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||
| Taxonomic identifier | 3702 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 134 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side By similarity. Microsome membrane; Single-pass membrane protein; Cytoplasmic side By similarity. |
| Post-translational modification | Ubiquitinated. Ref.7 |
| Sequence similarities | Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Electron transport Transport |
| Cellular component | Endoplasmic reticulum Membrane Microsome |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Heme Iron Metal-binding |
| PTM | Ubl conjugation |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transportInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR endoplasmic reticulum membraneNon-traceable author statement Ref.1. Source: TAIR integral to membraneInferred from electronic annotation. Source: UniProtKB-KW microsomeInferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from direct assay. Source: TAIR vacuolar membraneInferred from direct assay. Source: TAIR |
| Molecular function | heme binding Inferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 134 | 134 | Cytochrome b5 isoform 1 | PRO_0000166020 | |||||
Regions | |||||||||
| Transmembrane | 107 – 127 | 21 | Helical; Potential | ||||||
| Domain | 5 – 81 | 77 | Cytochrome b5 heme-binding | ||||||
Sites | |||||||||
| Metal binding | 40 | 1 | Iron (heme axial ligand) By similarity | ||||||
| Metal binding | 64 | 1 | Iron (heme axial ligand) By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 1 – 3 | 3 | MSS → ARA in CAA23377. Ref.6 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis." Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D. Plant Physiol. 119:353-361(1999) [PubMed: 9880378] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed: 9872454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [3] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [4] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [5] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [6] | "Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. Hoefte H.Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113. Strain: cv. Columbia. |
| [7] | "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed: 19292762] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AB007801 mRNA. Translation: BAA74839.1. AB015476 Genomic DNA. Translation: BAB09732.1. CP002688 Genomic DNA. Translation: AED96377.1. AY080878 mRNA. Translation: AAL87348.1. AY114045 mRNA. Translation: AAM45093.1. AY087070 mRNA. Translation: AAM64631.1. F20001 mRNA. Translation: CAA23377.1. |
| IPI | IPI00537177. |
| PIR | T52469. |
| RefSeq | NP_200168.1. NM_124736.3. |
| UniGene | At.20842. |
3D structure databases | |
| ProteinModelPortal | Q42342. |
| SMR | Q42342. Positions 2-84. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q42342. 9 interactions. |
| STRING | Q42342. |
Proteomic databases | |
| PRIDE | Q42342. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT5G53560.1; AT5G53560.1; AT5G53560. |
| GeneID | 835438. |
| GenomeReviews | Gene locus AT5G53560 in contig BA000015_GR. |
| KEGG | ath:AT5G53560. |
| NMPDR | fig|3702.1.peg.27252. |
Organism-specific databases | |
| TAIR | At5g53560. |
Phylogenomic databases | |
| GeneTree | EPGT00050000011061. |
| HOGENOM | HBG629516. |
| InParanoid | Q42342. |
| OMA | DTARDMM. |
| PhylomeDB | Q42342. |
| ProtClustDB | CLSN2686546. |
Gene expression databases | |
| ArrayExpress | Q42342. |
| Genevestigator | Q42342. |
| GermOnline | AT5G53560. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view] |
| Gene3D | G3DSA:3.10.120.10. Cyt_B5. 1 hit. |
| Pfam | PF00173. Cyt-b5. 1 hit. [Graphical view] |
| PRINTS | PR00363. CYTOCHROMEB5. |
| SUPFAM | SSF55856. Cyt_B5. 1 hit. |
| PROSITE | PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CYB51_ARATH | ||||||||
| Accession | Primary (citable) accession number: Q42342 Secondary accession number(s): Q9SB05 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

Clusters with