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Q42342

- CYB5A_ARATH

UniProt

Q42342 - CYB5A_ARATH

Protein

Cytochrome b5 isoform A

Gene

CYTB5-A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases, including fatty acid desaturases.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi40 – 401Iron (heme axial ligand)PROSITE-ProRule annotation
    Metal bindingi64 – 641Iron (heme axial ligand)PROSITE-ProRule annotation

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-KW

    Keywords - Biological processi

    Electron transport, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome b5 isoform A
    Alternative name(s):
    Cytochrome b5 isoform 1
    Gene namesi
    Name:CYTB5-A
    Synonyms:B5-A
    Ordered Locus Names:At5g53560
    ORF Names:MNC6.10
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G53560.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: TAIR
    2. endoplasmic reticulum Source: TAIR
    3. endoplasmic reticulum membrane Source: TAIR
    4. integral component of membrane Source: UniProtKB-KW
    5. plasma membrane Source: TAIR
    6. vacuolar membrane Source: TAIR
    7. vacuole Source: TAIR

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 134134Cytochrome b5 isoform APRO_0000166020Add
    BLAST

    Post-translational modificationi

    Ubiquitinated.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PaxDbiQ42342.
    PRIDEiQ42342.

    Expressioni

    Tissue specificityi

    Expressed in roots, stems, leaves, flowers and siliques.1 Publication

    Gene expression databases

    GenevestigatoriQ42342.

    Interactioni

    Subunit structurei

    Interacts with CER1, FAH1 and FAH2.2 Publications

    Protein-protein interaction databases

    BioGridi20682. 11 interactions.
    IntActiQ42342. 9 interactions.
    STRINGi3702.AT5G53560.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42342.
    SMRiQ42342. Positions 10-85.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei107 – 12721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini5 – 8177Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cytochrome b5 family.Curated
    Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5274.
    HOGENOMiHOG000039853.
    InParanoidiQ42342.
    OMAiYTISTTE.
    PhylomeDBiQ42342.

    Family and domain databases

    Gene3Di3.10.120.10. 1 hit.
    InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    [Graphical view]
    PfamiPF00173. Cyt-b5. 1 hit.
    [Graphical view]
    PRINTSiPR00363. CYTOCHROMEB5.
    SUPFAMiSSF55856. SSF55856. 1 hit.
    PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q42342-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS    50
    TGKDATNDFE DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN 100
    QDKTPEFIIK ILQFLVPILI LGLALVVRHY TKKD 134
    Length:134
    Mass (Da):15,084
    Last modified:January 11, 2001 - v2
    Checksum:i9CC01C60F7C873FD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 33MSS → ARA in CAA23377. (PubMed:8580968)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007801 mRNA. Translation: BAA74839.1.
    AB015476 Genomic DNA. Translation: BAB09732.1.
    CP002688 Genomic DNA. Translation: AED96377.1.
    AY080878 mRNA. Translation: AAL87348.1.
    AY114045 mRNA. Translation: AAM45093.1.
    AY087070 mRNA. Translation: AAM64631.1.
    F20001 mRNA. Translation: CAA23377.1.
    PIRiT52469.
    RefSeqiNP_200168.1. NM_124736.3.
    UniGeneiAt.20842.

    Genome annotation databases

    EnsemblPlantsiAT5G53560.1; AT5G53560.1; AT5G53560.
    GeneIDi835438.
    KEGGiath:AT5G53560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB007801 mRNA. Translation: BAA74839.1 .
    AB015476 Genomic DNA. Translation: BAB09732.1 .
    CP002688 Genomic DNA. Translation: AED96377.1 .
    AY080878 mRNA. Translation: AAL87348.1 .
    AY114045 mRNA. Translation: AAM45093.1 .
    AY087070 mRNA. Translation: AAM64631.1 .
    F20001 mRNA. Translation: CAA23377.1 .
    PIRi T52469.
    RefSeqi NP_200168.1. NM_124736.3.
    UniGenei At.20842.

    3D structure databases

    ProteinModelPortali Q42342.
    SMRi Q42342. Positions 10-85.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 20682. 11 interactions.
    IntActi Q42342. 9 interactions.
    STRINGi 3702.AT5G53560.1-P.

    Proteomic databases

    PaxDbi Q42342.
    PRIDEi Q42342.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G53560.1 ; AT5G53560.1 ; AT5G53560 .
    GeneIDi 835438.
    KEGGi ath:AT5G53560.

    Organism-specific databases

    TAIRi AT5G53560.

    Phylogenomic databases

    eggNOGi COG5274.
    HOGENOMi HOG000039853.
    InParanoidi Q42342.
    OMAi YTISTTE.
    PhylomeDBi Q42342.

    Miscellaneous databases

    PROi Q42342.

    Gene expression databases

    Genevestigatori Q42342.

    Family and domain databases

    Gene3Di 3.10.120.10. 1 hit.
    InterProi IPR001199. Cyt_B5-like_heme/steroid-bd.
    IPR018506. Cyt_B5_heme-BS.
    [Graphical view ]
    Pfami PF00173. Cyt-b5. 1 hit.
    [Graphical view ]
    PRINTSi PR00363. CYTOCHROMEB5.
    SUPFAMi SSF55856. SSF55856. 1 hit.
    PROSITEi PS00191. CYTOCHROME_B5_1. 1 hit.
    PS50255. CYTOCHROME_B5_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis."
      Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D.
      Plant Physiol. 119:353-361(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
      Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
      DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113.
      Strain: cv. Columbia.
    7. "Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)."
      Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M.
      Plant J. 58:122-134(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FAH1 AND FAH2.
    8. "Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
      Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
      Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
    9. "Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex."
      Bernard A., Domergue F., Pascal S., Jetter R., Renne C., Faure J.D., Haslam R.P., Napier J.A., Lessire R., Joubes J.
      Plant Cell 24:3106-3118(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CER1, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.
    10. "Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation."
      Kumar R., Tran L.S., Neelakandan A.K., Nguyen H.T.
      PLoS ONE 7:E31370-E31370(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCYB5A_ARATH
    AccessioniPrimary (citable) accession number: Q42342
    Secondary accession number(s): Q9SB05
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3