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Q42342 (CYB5A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome b5 isoform A
Alternative name(s):
Cytochrome b5 isoform 1
Gene names
Name:CYTB5-A
Synonyms:B5-A
Ordered Locus Names:At5g53560
ORF Names:MNC6.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length134 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases, including fatty acid desaturases. Ref.1 Ref.10

Subunit structure

Interacts with CER1, FAH1 and FAH2. Ref.7 Ref.9

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein Ref.9.

Tissue specificity

Expressed in roots, stems, leaves, flowers and siliques. Ref.1

Post-translational modification

Ubiquitinated.

Sequence similarities

Belongs to the cytochrome b5 family.

Contains 1 cytochrome b5 heme-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 134134Cytochrome b5 isoform A
PRO_0000166020

Regions

Transmembrane107 – 12721Helical; Potential
Domain5 – 8177Cytochrome b5 heme-binding

Sites

Metal binding401Iron (heme axial ligand) By similarity
Metal binding641Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict1 – 33MSS → ARA in CAA23377. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q42342 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 9CC01C60F7C873FD

FASTA13415,084
        10         20         30         40         50         60 
MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS TGKDATNDFE 

        70         80         90        100        110        120 
DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN QDKTPEFIIK ILQFLVPILI 

       130 
LGLALVVRHY TKKD 

« Hide

References

« Hide 'large scale' references
[1]"Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis."
Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D.
Plant Physiol. 119:353-361(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones."
Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S.
DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113.
Strain: cv. Columbia.
[7]"Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)."
Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M.
Plant J. 58:122-134(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FAH1 AND FAH2.
[8]"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis."
Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D.
Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS].
[9]"Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex."
Bernard A., Domergue F., Pascal S., Jetter R., Renne C., Faure J.D., Haslam R.P., Napier J.A., Lessire R., Joubes J.
Plant Cell 24:3106-3118(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CER1, SUBCELLULAR LOCATION.
Strain: cv. Columbia.
[10]"Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation."
Kumar R., Tran L.S., Neelakandan A.K., Nguyen H.T.
PLoS ONE 7:E31370-E31370(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB007801 mRNA. Translation: BAA74839.1.
AB015476 Genomic DNA. Translation: BAB09732.1.
CP002688 Genomic DNA. Translation: AED96377.1.
AY080878 mRNA. Translation: AAL87348.1.
AY114045 mRNA. Translation: AAM45093.1.
AY087070 mRNA. Translation: AAM64631.1.
F20001 mRNA. Translation: CAA23377.1.
PIRT52469.
RefSeqNP_200168.1. NM_124736.3.
UniGeneAt.20842.

3D structure databases

ProteinModelPortalQ42342.
SMRQ42342. Positions 10-85.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid20682. 11 interactions.
IntActQ42342. 9 interactions.
STRING3702.AT5G53560.1-P.

Proteomic databases

PaxDbQ42342.
PRIDEQ42342.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G53560.1; AT5G53560.1; AT5G53560.
GeneID835438.
KEGGath:AT5G53560.

Organism-specific databases

TAIRAT5G53560.

Phylogenomic databases

eggNOGCOG5274.
HOGENOMHOG000039853.
InParanoidQ42342.
OMARDMMEKY.
PhylomeDBQ42342.
ProtClustDBCLSN2686546.

Gene expression databases

GenevestigatorQ42342.

Family and domain databases

Gene3D3.10.120.10. 1 hit.
InterProIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSPR00363. CYTOCHROMEB5.
SUPFAMSSF55856. SSF55856. 1 hit.
PROSITEPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ42342.

Entry information

Entry nameCYB5A_ARATH
AccessionPrimary (citable) accession number: Q42342
Secondary accession number(s): Q9SB05
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: April 16, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names