Cytochrome b5 isoform A - Arabidopsis thaliana (Mouse-ear cress)

Contribute

Send feedback

Read comments (?) or add your own

Q42342 (CYB5A_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 114. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cytochrome b5 isoform A

Alternative name(s):
Cytochrome b5 isoform 1

Gene names

Name:	CYTB5-A
Synonyms:	B5-A
Ordered Locus Names:	At5g53560
ORF Names:	MNC6.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases, including fatty acid desaturases. Ref.1 Ref.10
Subunit structure	Interacts with CER1, FAH1 and FAH2. Ref.7 Ref.9
Subcellular location	Endoplasmic reticulum membrane; Single-pass membrane protein Ref.9.
Tissue specificity	Expressed in roots, stems, leaves, flowers and siliques. Ref.1
Post-translational modification	Ubiquitinated.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast thylakoid membrane Inferred from direct assay PubMed 15322131. Source: TAIR endoplasmic reticulum membrane Non-traceable author statement Ref.1. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay PubMed 15060130 PubMed 17644812. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 134

134

Cytochrome b5 isoform A

PRO_0000166020

Regions

Transmembrane

107 – 127

Helical; Potential

Domain

5 – 81

Cytochrome b5 heme-binding

Sites

Metal binding

Iron (heme axial ligand) By similarity

Metal binding

Iron (heme axial ligand) By similarity

Experimental info

Sequence conflict

1 – 3

MSS → ARA in CAA23377. Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

Q42342 [UniParc].

Last modified January 11, 2001. Version 2.
Checksum: 9CC01C60F7C873FD
Show »

FASTA

134

15,084

        10         20         30         40         50         60 
MSSDRKVLSF EEVSKHNKTK DCWLIISGKV YDVTPFMDDH PGGDEVLLSS TGKDATNDFE 

        70         80         90        100        110        120 
DVGHSDTARD MMDKYFIGEI DSSSVPATRT YVAPQQPAYN QDKTPEFIIK ILQFLVPILI 

       130 
LGLALVVRHY TKKD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Microsomal electron transfer in higher plants: cloning and heterologous expression of NADH-cytochrome b5 reductase from Arabidopsis." Fukuchi-Mizutani M., Mizutani M., Tanaka Y., Kusumi T., Ohta D. Plant Physiol. 119:353-361(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence features of the regions of 1,013,767 bp covered by sixteen physically assigned P1 and TAC clones." Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N., Tabata S. DNA Res. 5:297-308(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. Hoefte H. Plant J. 9:101-124(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-113. Strain: cv. Columbia.
[7]	"Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5)." Nagano M., Ihara-Ohori Y., Imai H., Inada N., Fujimoto M., Tsutsumi N., Uchimiya H., Kawai-Yamada M. Plant J. 58:122-134(2009) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FAH1 AND FAH2.
[8]	"Tandem affinity purification and mass spectrometric analysis of ubiquitylated proteins in Arabidopsis." Saracco S.A., Hansson M., Scalf M., Walker J.M., Smith L.M., Vierstra R.D. Plant J. 59:344-358(2009) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"Reconstitution of plant alkane biosynthesis in yeast demonstrates that Arabidopsis ECERIFERUM1 and ECERIFERUM3 are core components of a very-long-chain alkane synthesis complex." Bernard A., Domergue F., Pascal S., Jetter R., Renne C., Faure J.D., Haslam R.P., Napier J.A., Lessire R., Joubes J. Plant Cell 24:3106-3118(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CER1, SUBCELLULAR LOCATION. Strain: cv. Columbia.
[10]	"Higher plant cytochrome b5 polypeptides modulate fatty acid desaturation." Kumar R., Tran L.S., Neelakandan A.K., Nguyen H.T. PLoS ONE 7:E31370-E31370(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007801 mRNA. Translation: BAA74839.1. AB015476 Genomic DNA. Translation: BAB09732.1. CP002688 Genomic DNA. Translation: AED96377.1. AY080878 mRNA. Translation: AAL87348.1. AY114045 mRNA. Translation: AAM45093.1. AY087070 mRNA. Translation: AAM64631.1. F20001 mRNA. Translation: CAA23377.1.
IPI	IPI00537177.
PIR	T52469.
RefSeq	NP_200168.1. NM_124736.3.
UniGene	At.20842.
3D structure databases
ProteinModelPortal	Q42342.
SMR	Q42342. Positions 10-85.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42342. 9 interactions.
STRING	3702.AT5G53560.1-P.
Proteomic databases
PaxDb	Q42342.
PRIDE	Q42342.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G53560.1; AT5G53560.1; AT5G53560.
GeneID	835438.
KEGG	ath:AT5G53560.
Organism-specific databases
TAIR	At5g53560.
Phylogenomic databases
eggNOG	COG5274.
HOGENOM	HOG000039853.
InParanoid	Q42342.
OMA	RDMMEKY.
PhylomeDB	Q42342.
ProtClustDB	CLSN2686546.
Gene expression databases
ArrayExpress	Q42342.
Genevestigator	Q42342.
GermOnline	AT5G53560. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.10.120.10. 1 hit.
InterPro	IPR001199. Cyt_B5-like_heme/steroid-bd. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB5A_ARATH

Accession

Primary (citable) accession number: Q42342
Secondary accession number(s): Q9SB05

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 114 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents