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Q42328 (DF195_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Defensin-like protein 195
Alternative name(s):
ATTp
Trypsin inhibitor ATTI-1
diDi 4T-1
Gene names
Name:ATTI1
Ordered Locus Names:At2g43510
ORF Names:T01O24.25
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length89 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Secreted By similarity.

Sequence similarities

Belongs to the DEFL family. Protease inhibitor I18 (RTI/MTI-2) subfamily.

Caution

Was (Ref.1, Ref.3 and Ref.8) thought to be a protease inhibitor.

Sequence caution

The sequence CAA86849.1 differs from that shown. Reason: Frameshift at positions 10, 12, 15 and 23.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 8962Defensin-like protein 195
PRO_0000031093

Sites

Site48 – 492Reactive bond By similarity

Amino acid modifications

Disulfide bond32 ↔ 84 Ref.8
Disulfide bond45 ↔ 69 Ref.8
Disulfide bond54 ↔ 79 Ref.8
Disulfide bond58 ↔ 81 Ref.8

Natural variations

Natural variant61V → I in strain: cv. Goe-0.

Experimental info

Sequence conflict851D → G in CAA86849. Ref.1

Secondary structure

........... 89
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q42328 [UniParc].

Last modified January 10, 2003. Version 3.
Checksum: C4FA5D0B3ABB03D5

FASTA899,885
        10         20         30         40         50         60 
MAKAIVSIFV VFFIFFLVIS DVPEIEAQGN ECLKEYGGDV GFGFCAPRIF PTICYTRCRE 

        70         80 
NKGAKGGRCR WGQGSNVKCL CDFCDDTPQ 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of an Arabidopsis cDNA encoding a serine proteinase inhibitor ATTI-2."
Herve C., Tremousaygue D., Lescure B.
Plant Gene Register PGR95-011
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Arabidopsis thaliana genes expressed in the early compatible interaction with root-knot nematodes."
Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G.
Mol. Plant Microbe Interact. 14:288-299(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin inhibitor genes."
Clauss M.J., Mitchell-Olds T.
Genetics 166:1419-1436(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0, cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0, cv. Sah-0, cv. Ta-0, cv. Wassilewskija, cv. Wei-0 and cv. Wil-2.
Tissue: Leaf.
[4]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[7]"Genome organization of more than 300 defensin-like genes in Arabidopsis."
Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.
Plant Physiol. 138:600-610(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY.
[8]"NMR solution structure of ATTp, an Arabidopsis thaliana trypsin inhibitor."
Zhao Q., Chae Y.K., Markley J.L.
Biochemistry 41:12284-12296(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 23-89, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z46816 mRNA. Translation: CAA86849.1. Frameshift.
AJ249958 mRNA. Translation: CAB62548.1.
AJ632250 Genomic DNA. Translation: CAG15155.1.
AJ632251 Genomic DNA. Translation: CAG15160.1.
AJ632252 Genomic DNA. Translation: CAG15165.1.
AJ632253 Genomic DNA. Translation: CAG15170.1.
AJ632254 Genomic DNA. Translation: CAG15175.1.
AJ632255 Genomic DNA. Translation: CAG15181.1.
AJ632256 Genomic DNA. Translation: CAG15187.1.
AJ632257 Genomic DNA. Translation: CAG15193.1.
AJ632258 Genomic DNA. Translation: CAG15198.1.
AJ632259 Genomic DNA. Translation: CAG15203.1.
AJ632260 Genomic DNA. Translation: CAG15208.1.
AJ632261 Genomic DNA. Translation: CAG15213.1.
AJ632262 Genomic DNA. Translation: CAG15218.1.
AJ632263 Genomic DNA. Translation: CAG15223.1.
AJ632264 Genomic DNA. Translation: CAG15228.1.
AJ632265 Genomic DNA. Translation: CAG15233.1.
AJ632266 Genomic DNA. Translation: CAG15238.1.
AC002335 Genomic DNA. Translation: AAB64325.1.
CP002685 Genomic DNA. Translation: AEC10284.1.
AY050789 mRNA. Translation: AAK92724.1.
AY096456 mRNA. Translation: AAM20096.1.
PIRA84867.
RefSeqNP_181879.1. NM_129912.3.
UniGeneAt.10318.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JXCNMR-A22-89[»]
ProteinModelPortalQ42328.
SMRQ42328. Positions 23-89.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4288. 4 interactions.
IntActQ42328. 7 interactions.
STRING3702.AT2G43510.1-P.

Protein family/group databases

MEROPSI18.001.

Proteomic databases

PaxDbQ42328.
PRIDEQ42328.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G43510.1; AT2G43510.1; AT2G43510.
GeneID818952.
KEGGath:AT2G43510.

Organism-specific databases

TAIRAT2G43510.

Phylogenomic databases

eggNOGNOG276553.
HOGENOMHOG000090027.
OMAHKNINTA.
PhylomeDBQ42328.

Gene expression databases

GenevestigatorQ42328.

Family and domain databases

Gene3D3.30.30.10. 1 hit.
InterProIPR003614. Scorpion_toxin-like.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamPF00537. Toxin_3. 1 hit.
[Graphical view]
SMARTSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMSSF57095. SSF57095. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ42328.

Entry information

Entry nameDF195_ARATH
AccessionPrimary (citable) accession number: Q42328
Secondary accession number(s): O22865, Q6ZZP6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 10, 2003
Last modified: July 9, 2014
This is version 104 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names