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Q42328

- DF195_ARATH

UniProt

Q42328 - DF195_ARATH

Protein

Defensin-like protein 195

Gene

ATTI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei48 – 492Reactive bondBy similarity

    GO - Molecular functioni

    1. ion channel inhibitor activity Source: InterPro
    2. serine-type endopeptidase inhibitor activity Source: TAIR

    GO - Biological processi

    1. defense response Source: TAIR
    2. defense response to fungus Source: TAIR
    3. killing of cells of other organism Source: UniProtKB-KW
    4. negative regulation of endopeptidase activity Source: GOC

    Keywords - Molecular functioni

    Antimicrobial, Fungicide

    Keywords - Biological processi

    Plant defense

    Protein family/group databases

    MEROPSiI18.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Defensin-like protein 195
    Alternative name(s):
    ATTp
    Trypsin inhibitor ATTI-1
    diDi 4T-1
    Gene namesi
    Name:ATTI1
    Ordered Locus Names:At2g43510
    ORF Names:T01O24.25
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G43510.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Chaini28 – 8962Defensin-like protein 195PRO_0000031093Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi32 ↔ 841 Publication
    Disulfide bondi45 ↔ 691 Publication
    Disulfide bondi54 ↔ 791 Publication
    Disulfide bondi58 ↔ 811 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ42328.
    PRIDEiQ42328.

    Expressioni

    Gene expression databases

    GenevestigatoriQ42328.

    Interactioni

    Protein-protein interaction databases

    BioGridi4288. 4 interactions.
    IntActiQ42328. 7 interactions.
    STRINGi3702.AT2G43510.1-P.

    Structurei

    Secondary structure

    1
    89
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni42 – 443
    Helixi53 – 619
    Beta strandi65 – 695
    Beta strandi74 – 774
    Beta strandi79 – 835

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JXCNMR-A22-89[»]
    ProteinModelPortaliQ42328.
    SMRiQ42328. Positions 23-89.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ42328.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG276553.
    HOGENOMiHOG000090027.
    OMAiHKNINTA.
    PhylomeDBiQ42328.

    Family and domain databases

    Gene3Di3.30.30.10. 1 hit.
    InterProiIPR003614. Scorpion_toxin-like.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view]
    PfamiPF00537. Toxin_3. 1 hit.
    [Graphical view]
    SMARTiSM00505. Knot1. 1 hit.
    [Graphical view]
    SUPFAMiSSF57095. SSF57095. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q42328-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKAIVSIFV VFFIFFLVIS DVPEIEAQGN ECLKEYGGDV GFGFCAPRIF   50
    PTICYTRCRE NKGAKGGRCR WGQGSNVKCL CDFCDDTPQ 89
    Length:89
    Mass (Da):9,885
    Last modified:January 10, 2003 - v3
    Checksum:iC4FA5D0B3ABB03D5
    GO

    Sequence cautioni

    The sequence CAA86849.1 differs from that shown. Reason: Frameshift at positions 10, 12, 15 and 23.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti85 – 851D → G in CAA86849. 1 PublicationCurated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti6 – 61V → I in strain: cv. Goe-0.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46816 mRNA. Translation: CAA86849.1. Frameshift.
    AJ249958 mRNA. Translation: CAB62548.1.
    AJ632250 Genomic DNA. Translation: CAG15155.1.
    AJ632251 Genomic DNA. Translation: CAG15160.1.
    AJ632252 Genomic DNA. Translation: CAG15165.1.
    AJ632253 Genomic DNA. Translation: CAG15170.1.
    AJ632254 Genomic DNA. Translation: CAG15175.1.
    AJ632255 Genomic DNA. Translation: CAG15181.1.
    AJ632256 Genomic DNA. Translation: CAG15187.1.
    AJ632257 Genomic DNA. Translation: CAG15193.1.
    AJ632258 Genomic DNA. Translation: CAG15198.1.
    AJ632259 Genomic DNA. Translation: CAG15203.1.
    AJ632260 Genomic DNA. Translation: CAG15208.1.
    AJ632261 Genomic DNA. Translation: CAG15213.1.
    AJ632262 Genomic DNA. Translation: CAG15218.1.
    AJ632263 Genomic DNA. Translation: CAG15223.1.
    AJ632264 Genomic DNA. Translation: CAG15228.1.
    AJ632265 Genomic DNA. Translation: CAG15233.1.
    AJ632266 Genomic DNA. Translation: CAG15238.1.
    AC002335 Genomic DNA. Translation: AAB64325.1.
    CP002685 Genomic DNA. Translation: AEC10284.1.
    AY050789 mRNA. Translation: AAK92724.1.
    AY096456 mRNA. Translation: AAM20096.1.
    PIRiA84867.
    RefSeqiNP_181879.1. NM_129912.3.
    UniGeneiAt.10318.

    Genome annotation databases

    EnsemblPlantsiAT2G43510.1; AT2G43510.1; AT2G43510.
    GeneIDi818952.
    KEGGiath:AT2G43510.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z46816 mRNA. Translation: CAA86849.1 . Frameshift.
    AJ249958 mRNA. Translation: CAB62548.1 .
    AJ632250 Genomic DNA. Translation: CAG15155.1 .
    AJ632251 Genomic DNA. Translation: CAG15160.1 .
    AJ632252 Genomic DNA. Translation: CAG15165.1 .
    AJ632253 Genomic DNA. Translation: CAG15170.1 .
    AJ632254 Genomic DNA. Translation: CAG15175.1 .
    AJ632255 Genomic DNA. Translation: CAG15181.1 .
    AJ632256 Genomic DNA. Translation: CAG15187.1 .
    AJ632257 Genomic DNA. Translation: CAG15193.1 .
    AJ632258 Genomic DNA. Translation: CAG15198.1 .
    AJ632259 Genomic DNA. Translation: CAG15203.1 .
    AJ632260 Genomic DNA. Translation: CAG15208.1 .
    AJ632261 Genomic DNA. Translation: CAG15213.1 .
    AJ632262 Genomic DNA. Translation: CAG15218.1 .
    AJ632263 Genomic DNA. Translation: CAG15223.1 .
    AJ632264 Genomic DNA. Translation: CAG15228.1 .
    AJ632265 Genomic DNA. Translation: CAG15233.1 .
    AJ632266 Genomic DNA. Translation: CAG15238.1 .
    AC002335 Genomic DNA. Translation: AAB64325.1 .
    CP002685 Genomic DNA. Translation: AEC10284.1 .
    AY050789 mRNA. Translation: AAK92724.1 .
    AY096456 mRNA. Translation: AAM20096.1 .
    PIRi A84867.
    RefSeqi NP_181879.1. NM_129912.3.
    UniGenei At.10318.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JXC NMR - A 22-89 [» ]
    ProteinModelPortali Q42328.
    SMRi Q42328. Positions 23-89.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4288. 4 interactions.
    IntActi Q42328. 7 interactions.
    STRINGi 3702.AT2G43510.1-P.

    Protein family/group databases

    MEROPSi I18.001.

    Proteomic databases

    PaxDbi Q42328.
    PRIDEi Q42328.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G43510.1 ; AT2G43510.1 ; AT2G43510 .
    GeneIDi 818952.
    KEGGi ath:AT2G43510.

    Organism-specific databases

    TAIRi AT2G43510.

    Phylogenomic databases

    eggNOGi NOG276553.
    HOGENOMi HOG000090027.
    OMAi HKNINTA.
    PhylomeDBi Q42328.

    Miscellaneous databases

    EvolutionaryTracei Q42328.

    Gene expression databases

    Genevestigatori Q42328.

    Family and domain databases

    Gene3Di 3.30.30.10. 1 hit.
    InterProi IPR003614. Scorpion_toxin-like.
    IPR002061. Scorpion_toxinL/defensin.
    [Graphical view ]
    Pfami PF00537. Toxin_3. 1 hit.
    [Graphical view ]
    SMARTi SM00505. Knot1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57095. SSF57095. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of an Arabidopsis cDNA encoding a serine proteinase inhibitor ATTI-2."
      Herve C., Tremousaygue D., Lescure B.
      Plant Gene Register PGR95-011
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Arabidopsis thaliana genes expressed in the early compatible interaction with root-knot nematodes."
      Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G.
      Mol. Plant Microbe Interact. 14:288-299(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Functional divergence in tandemly duplicated Arabidopsis thaliana trypsin inhibitor genes."
      Clauss M.J., Mitchell-Olds T.
      Genetics 166:1419-1436(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], GENE FAMILY, NOMENCLATURE.
      Strain: cv. Col-1, cv. Cvi-1, cv. Di-0, cv. Fe-1a, cv. Goe-0, cv. Ita-0, cv. Kas-1, cv. Landsberg erecta, cv. Le-0, cv. Nd-1, cv. Nok-0, cv. Rsch-0, cv. Sah-0, cv. Ta-0, cv. Wassilewskija, cv. Wei-0 and cv. Wil-2.
      Tissue: Leaf.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    5. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    6. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    7. "Genome organization of more than 300 defensin-like genes in Arabidopsis."
      Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.
      Plant Physiol. 138:600-610(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY.
    8. "NMR solution structure of ATTp, an Arabidopsis thaliana trypsin inhibitor."
      Zhao Q., Chae Y.K., Markley J.L.
      Biochemistry 41:12284-12296(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 23-89, DISULFIDE BONDS.

    Entry informationi

    Entry nameiDF195_ARATH
    AccessioniPrimary (citable) accession number: Q42328
    Secondary accession number(s): O22865, Q6ZZP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Caution

    Was (PubMed:11277426, PubMed:15082560 and PubMed:12369816) thought to be a protease inhibitor.Curated

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3