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Reviewed, UniProtKB/Swiss-Prot Q42290 (MPPB_ARATH)

Last modified June 16, 2009. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Probable mitochondrial-processing peptidase subunit beta
    EC=3.4.24.64
Alternative name(s):
    Beta-MPP
Gene names
Ordered Locus Names: At3g02090
ORF Names: F1C9.12
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Cleaves presequences (transit peptides) from mitochondrial protein precursors By similarity.

Catalytic activity

Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Heterodimer of alpha and beta subunits By similarity.

Subcellular location

Mitochondrion. Ref.4

Sequence similarities

Belongs to the peptidase M16 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q42290-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q42290-2)

The sequence of this isoform differs from the canonical sequence as follows:
     504-531: DIAISAIGPIQDLPDYNKFRRRTYWNRY → VRHCNLSYWSNPRFARLQQIQTQNLLEPVLRL
Note: Derived from EST data. May be due to a competing donor splice site. No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2525Mitochondrion Potential
Chain26 – 531506Probable mitochondrial-processing peptidase subunit beta
PRO_0000045852

Regions

Compositional bias25 – 306Poly-Ser
Compositional bias46 – 494Poly-Pro

Sites

Active site1441Proton acceptor By similarity
Active site2141 By similarity
Metal binding1411Zinc By similarity
Metal binding1451Zinc By similarity
Metal binding2211Zinc By similarity

Natural variations

Alternative sequence504 – 53128DIAIS…YWNRY → VRHCNLSYWSNPRFARLQQI QTQNLLEPVLRL in isoform 2.
VSP_018097

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: 08A3953AD192E6B5

FASTA53159,160
        10         20         30         40         50         60 
MAMKNLLSLA RRSQRRLFLT QATRSSSSFS AIDSVPASAS PTALSPPPPH LMPYDHAAEI 

        70         80         90        100        110        120 
IKNKIKKLEN PDKRFLKYAS PHPILASHNH ILSAPETRVT TLPNGLRVAT ESNLSAKTAT 

       130        140        150        160        170        180 
VGVWIDAGSR FESDETNGTA HFLEHMIFKG TDRRTVRALE EEIEDIGGHL NAYTSREQTT 

       190        200        210        220        230        240 
YYAKVLDSNV NQALDVLADI LQNSKFEEQR INRERDVILR EMQEVEGQTD EVVLDHLHAT 

       250        260        270        280        290        300 
AFQYTPLGRT ILGPAQNVKS ITREDLQNYI KTHYTASRMV IAAAGAVKHE EVVEQVKKLF 

       310        320        330        340        350        360 
TKLSSDPTTT SQLVANEPAS FTGSEVRMID DDLPLAQFAV AFEGASWTDP DSVALMVMQT 

       370        380        390        400        410        420 
MLGSWNKNVG GGKHVGSDLT QRVAINEIAE SIMAFNTNYK DTGLFGVYAV AKADCLDDLS 

       430        440        450        460        470        480 
YAIMYEVTKL AYRVSDADVT RARNQLKSSL LLHMDGTSPI AEDIGRQLLT YGRRIPTAEL 

       490        500        510        520        530 
FARIDAVDAS TVKRVANKYI YDKDIAISAI GPIQDLPDYN KFRRRTYWNR Y 

« Hide

Isoform 2.

Checksum: E5D83B408D218D8A
Show »

FASTA53559,612

References

[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: cv. Columbia.
[3]"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs."
Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. expand/collapse author list , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.
Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 321-421.
Strain: cv. Columbia.
Tissue: Leaf.
[4]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

AC011664 Genomic DNA. Translation: AAF14827.1.
AY126990 mRNA. Translation: AAM83217.1.
BT000830 mRNA. Translation: AAN33205.1.
BT000662 mRNA. Translation: AAN31809.1.
BT001915 mRNA. Translation: AAN71914.1.
Z35354 mRNA. Translation: CAA84561.1.
IPIIPI00530627.
IPI00539775.
RefSeqNP_186858.1.
NP_850500.1.
UniGeneAt.23364
At.69050

3D structure databases

HSSPHSSP built from PDB template 1HR6 based on UniProtKB P10507.
ModBaseSearch...

Protein family/group databases

MEROPSM16.003.

Proteomic databases

PRIDEQ42290.

Genome annotation databases

GeneID821084.
GenomeReviewsGene locus AT3G02090 in contig BA000014_GR.
KEGGath:AT3G02090.
NMPDRfig|3702.1.peg.12145.

Organism-specific databases

GeneFarm2127. 202.
TAIRAt3g02090.

Phylogenomic databases

OMAQ42290. FAQSGAY.

Enzyme and pathway databases

BRENDA3.4.24.64. 302.

Gene expression databases

GermOnlineAT3G02090. Arabidopsis thaliana.

Family and domain databases

InterProIPR011237. Pept_M16_core.
IPR011765. Pept_M16_N.
IPR001431. Pept_M16_Zn_BS.
IPR007863. Peptidase_M16_C.
[Graphical view]
Gene3DG3DSA:3.30.830.10. Pept_M16_core. 2 hits.
PfamPF00675. Peptidase_M16. 1 hit.
PF05193. Peptidase_M16_C. 1 hit.
[Graphical view]
PROSITEPS00143. INSULINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPPB_ARATH
AccessionPrimary (citable) accession number: Q42290
Secondary accession number(s): Q9SGA7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents