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Protein

Proteasome subunit alpha type-5-B

Gene

PAE2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.PROSITE-ProRule annotation

GO - Molecular functioni

  • ribonuclease activity Source: TAIR
  • threonine-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.995.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit alpha type-5-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome alpha subunit E-2
Proteasome component Z
Gene namesi
Name:PAE2
Synonyms:PRCZ
Ordered Locus Names:At3g14290
ORF Names:MLN21.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G14290.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • cytosolic ribosome Source: TAIR
  • nucleus Source: UniProtKB-SubCell
  • proteasome complex Source: TAIR
  • proteasome core complex, alpha-subunit complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001241251 – 237Proteasome subunit alpha type-5-BAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Cross-linki43Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki66Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ42134.
PRIDEiQ42134.

Expressioni

Gene expression databases

GenevisibleiQ42134. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.3 Publications

Protein-protein interaction databases

BioGridi5983. 2 interactors.
IntActiQ42134. 1 interactor.
STRINGi3702.AT3G14290.1.

Structurei

3D structure databases

ProteinModelPortaliQ42134.
SMRiQ42134.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1A family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0176. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000091085.
KOiK02729.
OMAiELQNEFH.
OrthoDBiEOG09360K8Y.
PhylomeDBiQ42134.

Family and domain databases

CDDicd03753. proteasome_alpha_type_5. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR033812. Proteasome_alpha_type_5.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42134-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGV KTKEGVVLAV
60 70 80 90 100
EKRITSPLLE PSSVEKIMEI DDHIGCAMSG LIADARTLVE HARVETQNHR
110 120 130 140 150
FSYGEPMTVE STTQALCDLA LRFGEGEEES MSRPFGVSLL IAGHDENGPS
160 170 180 190 200
LYYTDPSGTF WQCNAKAIGS GSEGADSSLQ EQFNKDITLQ EAETIAVSIL
210 220 230
KQVMEEKVTP NNVDIAKVAP AYHLYTPQEV EAVISRL
Length:237
Mass (Da):25,977
Last modified:May 1, 1999 - v2
Checksum:iB178A3A0DDA12680
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043525 mRNA. Translation: AAC32061.1.
AB022220 Genomic DNA. Translation: BAB01035.1.
CP002686 Genomic DNA. Translation: AEE75498.1.
AF370245 mRNA. Translation: AAK44060.1.
AY062970 mRNA. Translation: AAL33816.1.
Z26556 mRNA. Translation: CAA81327.1.
PIRiT51973.
RefSeqiNP_188046.1. NM_112287.4.
UniGeneiAt.21306.

Genome annotation databases

EnsemblPlantsiAT3G14290.1; AT3G14290.1; AT3G14290.
GeneIDi820649.
GrameneiAT3G14290.1; AT3G14290.1; AT3G14290.
KEGGiath:AT3G14290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043525 mRNA. Translation: AAC32061.1.
AB022220 Genomic DNA. Translation: BAB01035.1.
CP002686 Genomic DNA. Translation: AEE75498.1.
AF370245 mRNA. Translation: AAK44060.1.
AY062970 mRNA. Translation: AAL33816.1.
Z26556 mRNA. Translation: CAA81327.1.
PIRiT51973.
RefSeqiNP_188046.1. NM_112287.4.
UniGeneiAt.21306.

3D structure databases

ProteinModelPortaliQ42134.
SMRiQ42134.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5983. 2 interactors.
IntActiQ42134. 1 interactor.
STRINGi3702.AT3G14290.1.

Protein family/group databases

MEROPSiT01.995.

Proteomic databases

PaxDbiQ42134.
PRIDEiQ42134.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G14290.1; AT3G14290.1; AT3G14290.
GeneIDi820649.
GrameneiAT3G14290.1; AT3G14290.1; AT3G14290.
KEGGiath:AT3G14290.

Organism-specific databases

TAIRiAT3G14290.

Phylogenomic databases

eggNOGiKOG0176. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000091085.
KOiK02729.
OMAiELQNEFH.
OrthoDBiEOG09360K8Y.
PhylomeDBiQ42134.

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ42134.

Gene expression databases

GenevisibleiQ42134. AT.

Family and domain databases

CDDicd03753. proteasome_alpha_type_5. 1 hit.
Gene3Di3.60.20.10. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR033812. Proteasome_alpha_type_5.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTiSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSA5B_ARATH
AccessioniPrimary (citable) accession number: Q42134
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.