Q42093 (AB2C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABC transporter C family member 2
Short name=ABC transporter ABCC.2
Short name=AtABCC2
EC=3.6.3.44

Alternative name(s):
ATP-energized glutathione S-conjugate pump 2
Glutathione S-conjugate-transporting ATPase 2
Multidrug resistance-associated protein 2

Gene names

Name:	ABCC2
Synonyms:	EST4, MRP2
Ordered Locus Names:	At2g34660
ORF Names:	T29F13.13

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1623 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pump for glutathione S-conjugates. Mediates the transport of S-conjugates such as GSH, S-(2,4-dinitrophenyl)-glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS (MOC-GS), glucuronides such as 17-beta-estradiol 17-(beta-D-glucuronide) (E217betaG), and of the chlorophyll catabolite such as B.napus nonfluorescent chlorophyll catabolite (Bn-NCC-1). Ref.2 Ref.7
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Enzyme regulation	Reciprocal promotion of DNP-GS and E217betaG uptake. E217betaG uptake is also stimulated by GSH and S-methyl-glutathione (S-methyl-GS), and, to a lower extent, by GSSG and C3G-GS. Metolachlor-GS and decyl-GS slightly inhibit E217betaG uptake. Ref.7
Subunit structure	Interacts with FKBP42/TWD1 and probably with calmodulin (CaM). Ref.11
Subcellular location	Vacuole membrane; Multi-pass membrane protein Potential. Note: Tonoplast. Ref.7
Tissue specificity	Ubiquitous, at low levels. Ref.2 Ref.10
Induction	By 1-chloro-2,4-dinitrobenzene (CDNB). Ref.6 Ref.7
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Biophysicochemical properties	Kinetic parameters: KM=750 µM for E217betaG (at pH 8.0 and 25 degrees Celsius) Ref.2 Ref.7 KM=240 µM for E217betaG (with 100 µM DNP-GS at pH 8.0 and 25 degrees Celsius) KM=4.7 µM for E217betaG (with 500 µM GSH at pH 8.0 and 25 degrees Celsius) KM=66 µM for DNP-GS (at pH 8) KM=20 µM for DNP-GS (with 100 µM E217betaG at pH 8 and 25 degrees Celsius) KM=73 µM for GSSG (at pH 8) KM=75 µM for MOC-GS (at pH 8) KM=15 µM for Bn-NCC-1 (at pH 8) Vmax=8.8 nmol/min/mg enzyme with E217betaG as substrate (at pH 8.0 and 25 degrees Celsius) Vmax=11.5 nmol/min/mg enzyme with E217betaG as substrate (with 100 µM DNP-GS at pH 8.0 and 25 degrees Celsius) Vmax=33 nmol/min/mg enzyme with E217betaG as substrate (with 500 µM GSH at pH 8.0 and 25 degrees Celsius) Vmax=2 nmol/min/mg enzyme with DNP-GS as substrate (at pH 8 and 25 degrees Celsius) Vmax=1.8 nmol/min/mg enzyme with DNP-GS as substrate (with 100 µM E217betaG at pH 8 and 25 degrees Celsius) Vmax=3.8 nmol/min/mg enzyme with GSSG as substrate (at pH 8) Vmax=13.6 nmol/min/mg enzyme with MOC-GS as substrate (at pH 8) Vmax=6.3 nmol/min/mg enzyme with Bn-NCC-1 as substrate (at pH 8)
Sequence caution	The sequence AAC04246.1 differs from that shown. Reason: Frameshift at positions 1212 and 1223. The sequence AAC49798.1 differs from that shown. Reason: Frameshift at position 1461. The sequence CAA81250.1 differs from that shown. Reason: Frameshift at positions 1223 and 1234.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1623	1623	ABC transporter C family member 2		PRO_0000226073
Regions
	Transmembrane	37 – 57	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	109 – 129	21	Helical; Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Transmembrane	172 – 192	21	Helical; Potential
	Transmembrane	336 – 356	21	Helical; Potential
	Transmembrane	440 – 460	21	Helical; Potential
	Transmembrane	527 – 547	21	Helical; Potential
	Transmembrane	557 – 577	21	Helical; Potential
	Transmembrane	914 – 934	21	Helical; Potential
	Transmembrane	955 – 975	21	Helical; Potential
	Transmembrane	1032 – 1054	23	Helical; Potential
	Transmembrane	1058 – 1077	20	Helical; Potential
	Transmembrane	1143 – 1163	21	Helical; Potential
	Transmembrane	1177 – 1197	21	Helical; Potential
	Domain	302 – 582	281	ABC transmembrane type-1 1
	Domain	614 – 838	225	ABC transporter 1
	Domain	921 – 1205	285	ABC transmembrane type-1 2
	Domain	1242 – 1476	235	ABC transporter 2
	Nucleotide binding	649 – 656	8	ATP 1 Potential
	Nucleotide binding	1276 – 1283	8	ATP 2 Potential
	Region	1236 – 1251	16	Interaction with calmodulin and FKP42/TWD1 By similarity
Experimental info
	Sequence conflict	21	1	Q → T in AAC04245. Ref.2
	Sequence conflict	21	1	Q → T in AAC04246. Ref.2
	Sequence conflict	57	1	A → T in AAC49988. Ref.1
	Sequence conflict	141	1	W → C in AAC49988. Ref.1
	Sequence conflict	193	1	H → R in AAC49988. Ref.1
	Sequence conflict	231	1	A → P in AAC04245. Ref.2
	Sequence conflict	348	1	V → G in AAC04245. Ref.2
	Sequence conflict	348	1	V → G in AAC04246. Ref.2
	Sequence conflict	380	1	F → S in AAC49988. Ref.1
	Sequence conflict	588	1	K → N in AAC04245. Ref.2
	Sequence conflict	835	1	L → V in AAC04246. Ref.2
	Sequence conflict	1250	1	R → C in AAC49988. Ref.1
	Sequence conflict	1294 – 1295	2	EV → GG in CAA81250. Ref.5
	Sequence conflict	1297	1	K → E in AAC04245. Ref.2
	Sequence conflict	1392	1	A → G in AAC49988. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q42093 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: F074F0F6ED7A4D47	FASTA	1,623	182,130
	10 20 30 40 50 60 MGFEFIEWYC KPVPNGVWTK QVANAFGAYT PCATDSFVLG ISQLVLLVLC LYRIWLALKD 70 80 90 100 110 120 HKVERFCLRS RLYNYFLALL AAYATAEPLF RLIMGISVLD FDGPGLPPFE AFGLGVKAFA 130 140 150 160 170 180 WGAVMVMILM ETKIYIRELR WYVRFAVIYA LVGDMVLLNL VLSVKEYYSS YVLYLYTSEV 190 200 210 220 230 240 GAQVLFGILL FMHLPNLDTY PGYMPVRSET VDDYEYEEIS DGQQICPEKH ANIFDKIFFS 250 260 270 280 290 300 WMNPLMTLGS KRPLTEKDVW YLDTWDQTET LFTSFQHSWD KELQKPQPWL LRALNNSLGG 310 320 330 340 350 360 RFWWGGFWKI GNDCSQFVGP LLLNQLLKSM QEDAPAWMGY IYAFSIFVGV VFGVLCEAQY 370 380 390 400 410 420 FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RRKFQTGKIT NLMTTDAESL QQICQSLHTM 430 440 450 460 470 480 WSAPFRIIIA LILLYQQLGV ASLIGALLLV LMFPLQTVII SKMQKLTKEG LQRTDKRIGL 490 500 510 520 530 540 MNEVLAAMDT VKCYAWENSF QSKVQTVRDD ELSWFRKSQL LGALNMFILN SIPVLVTIVS 550 560 570 580 590 600 FGVFTLLGGD LTPARAFTSL SLFAVLRFPL FMLPNIITQV VNANVSLKRL EEVLATEERI 610 620 630 640 650 660 LLPNPPIEPG EPAISIRNGY FSWDSKGDRP TLSNINLDVP LGSLVAVVGS TGEGKTSLIS 670 680 690 700 710 720 AILGELPATS DAIVTLRGSV AYVPQVSWIF NATVRDNILF GSPFDREKYE RAIDVTSLKH 730 740 750 760 770 780 DLELLPGGDL TEIGERGVNI SGGQKQRVSM ARAVYSNSDV YIFDDPLSAL DAHVGQQVFE 790 800 810 820 830 840 KCIKRELGQK TRVLVTNQLH FLSQVDRIVL VHEGTVKEEG TYEELSSNGP LFQRLMENAG 850 860 870 880 890 900 KVEEYSEENG EAEADQTAEQ PVANGNTNGL QMDGSDDKKS KEGNKKGGKS VLIKQEERET 910 920 930 940 950 960 GVVSWRVLKR YQDALGGAWV VMMLLLCYVL TEVFRVTSST WLSEWTDAGT PKSHGPLFYN 970 980 990 1000 1010 1020 LIYALLSFGQ VLVTLTNSYW LIMSSLYAAK KLHDNMLHSI LRAPMSFFHT NPLGRIINRF 1030 1040 1050 1060 1070 1080 AKDLGDIDRT VAVFVNMFMG QVSQLLSTVV LIGIVSTLSL WAIMPLLVLF YGAYLYYQNT 1090 1100 1110 1120 1130 1140 AREVKRMDSI SRSPVYAQFG EALNGLSTIR AYKAYDRMAD INGRSMDNNI RFTLVNMGAN 1150 1160 1170 1180 1190 1200 RWLGIRLETL GGLMIWLTAS FAVMQNGRAE NQQAFASTMG LLLSYALNIT SLLTGVLRLA 1210 1220 1230 1240 1250 1260 SLAENSLNAV ERVGNYIEIP PEAPPVIENN RPPPGWPSSG SIKFEDVVLR YRPQLPPVLH 1270 1280 1290 1300 1310 1320 GVSFFIHPTD KVGIVGRTGA GKSSLLNALF RIVEVEKGRI LIDDCDVGKF GLMDLRKVLG 1330 1340 1350 1360 1370 1380 IIPQSPVLFS GTVRFNLDPF GEHNDADLWE SLERAHLKDT IRRNPLGLDA EVSEAGENFS 1390 1400 1410 1420 1430 1440 VGQRQLLSLS RALLRRSKIL VLDEATAAVD VRTDALIQKT IREEFKSCTM LIIAHRLNTI 1450 1460 1470 1480 1490 1500 IDCDKILVLD SGRVQEFSSP ENLLSNEGSS FSKMVQSTGA ANAEYLRSLV LDNKRAKDDS 1510 1520 1530 1540 1550 1560 HHLQGQRKWL ASSRWAAAAQ FALAASLTSS HNDLQSLEIE DDSSILKRTN DAVVTLRSVL 1570 1580 1590 1600 1610 1620 EGKHDKEIAE SLEEHNISRE GWLSSLYRMV EGLAVMSRLA RNRMQQPDYN FEGNTFDWDN VEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of AtMRP1, an Arabidopsis thaliana cDNA encoding a homologue of the mammalian multidrug resistance-associated protein." Marin E., Leonhardt N., Vavasseur A., Forestier C. Biochim. Biophys. Acta 1369:7-13(1998) [PubMed: 9528668] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"AtMRP2, an Arabidopsis ATP-binding cassette transporter able to transport glutathione S-conjugates and chlorophyll catabolites: functional comparisons with AtMRP1." Lu Y.-P., Li Z.-S., Drozdowicz Y.M., Hoertensteiner S., Martinoia E., Rea P.A. Plant Cell 10:267-282(1998) [PubMed: 9490749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	Desprez T., Amselem J., Chiapello H., Caboche M., Hofte H. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1174-1295. Strain: cv. Columbia. Tissue: Seedling.
[6]	"Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics." Tommasini R., Vogt E., Schmid J., Fromentau M., Amrhein N., Martinoia E. FEBS Lett. 411:206-210(1997) [PubMed: 9271206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1182-1491, INDUCTION.
[7]	"Enhanced multispecificity of Arabidopsis vacuolar multidrug resistance-associated protein-type ATP-binding cassette transporter, AtMRP2." Liu G., Sanchez-Fernandez R., Li Z.-S., Rea P.A. J. Biol. Chem. 276:8648-8656(2001) [PubMed: 11115509] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
[8]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[9]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: GENE FAMILY.
[10]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY.
[11]	"Arabidopsis immunophilin-like TWD1 functionally interacts with vacuolar ABC transporters." Geisler M., Girin M., Brandt S., Vincenzetti V., Plaza S., Paris N., Kobae Y., Maeshima M., Billion K., Kolukisaoglu U.H., Schulz B., Martinoia E. Mol. Biol. Cell 15:3393-3405(2004) [PubMed: 15133126] [Abstract] Cited for: INTERACTION WITH FKBP42/TWD1.
[12]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF014960 mRNA. Translation: AAC49988.1. AF020288 mRNA. Translation: AAC04245.1. AF020289 Genomic DNA. Translation: AAC04246.1. Frameshift. AC003096 Genomic DNA. Translation: AAC16268.1. CP002685 Genomic DNA. Translation: AEC09005.1. CP002685 Genomic DNA. Translation: AEC09006.1. Z26467 mRNA. Translation: CAA81250.1. Frameshift. U96400 mRNA. Translation: AAC49798.1. Frameshift.
IPI	IPI00528135.
PIR	T01369.
RefSeq	NP_001189675.1. NM_001202746.1. NP_181013.1. NM_129020.3.
UniGene	At.19665.
3D structure databases
ProteinModelPortal	Q42093.
SMR	Q42093. Positions 291-839, 905-1478.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q42093. 1 interaction.
STRING	Q42093.
Protein family/group databases
TCDB	3.A.1.208.5. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	Q42093.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G34660.1; AT2G34660.1; AT2G34660. AT2G34660.2; AT2G34660.2; AT2G34660.
GeneID	818031.
GenomeReviews	Gene locus AT2G34660 in contig CT485783_GR.
KEGG	ath:AT2G34660.
NMPDR	fig|3702.1.peg.10564.
Organism-specific databases
TAIR	At2g34660.
Phylogenomic databases
eggNOG	KOG0054.
GeneTree	EPGT00070000028299.
HOGENOM	HBG758042.
InParanoid	Q42093.
OMA	TVIISKM.
PhylomeDB	Q42093.
ProtClustDB	PLN03130.
Gene expression databases
Genevestigator	Q42093.
GermOnline	AT2G34660. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB2C_ARATH

Accession

Primary (citable) accession number: Q42093
Secondary accession number(s): O22449 , O24526, O48907, O48908, O64590

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	November 16, 2011
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program