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Protein

ABC transporter C family member 2

Gene

ABCC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pump for glutathione S-conjugates. Mediates the transport of S-conjugates such as GSH, S-(2,4-dinitrophenyl)-glutathione (DNP-GS), GSSG, cyanidin 3-glucoside-GS (C3G-GS) and metolachlor-GS (MOC-GS), glucuronides such as 17-beta-estradiol 17-(beta-D-glucuronide) (E217betaG), and of the chlorophyll catabolite such as B.napus nonfluorescent chlorophyll catabolite (Bn-NCC-1).2 Publications

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Enzyme regulationi

Reciprocal promotion of DNP-GS and E217betaG uptake. E217betaG uptake is also stimulated by GSH and S-methyl-glutathione (S-methyl-GS), and, to a lower extent, by GSSG and C3G-GS. Metolachlor-GS and decyl-GS slightly inhibit E217betaG uptake.1 Publication

Kineticsi

  1. KM=750 µM for E217betaG (at pH 8.0 and 25 degrees Celsius)2 Publications
  2. KM=240 µM for E217betaG (with 100 µM DNP-GS at pH 8.0 and 25 degrees Celsius)2 Publications
  3. KM=4.7 µM for E217betaG (with 500 µM GSH at pH 8.0 and 25 degrees Celsius)2 Publications
  4. KM=66 µM for DNP-GS (at pH 8)2 Publications
  5. KM=20 µM for DNP-GS (with 100 µM E217betaG at pH 8 and 25 degrees Celsius)2 Publications
  6. KM=73 µM for GSSG (at pH 8)2 Publications
  7. KM=75 µM for MOC-GS (at pH 8)2 Publications
  8. KM=15 µM for Bn-NCC-1 (at pH 8)2 Publications
  1. Vmax=8.8 nmol/min/mg enzyme with E217betaG as substrate (at pH 8.0 and 25 degrees Celsius)2 Publications
  2. Vmax=11.5 nmol/min/mg enzyme with E217betaG as substrate (with 100 µM DNP-GS at pH 8.0 and 25 degrees Celsius)2 Publications
  3. Vmax=33 nmol/min/mg enzyme with E217betaG as substrate (with 500 µM GSH at pH 8.0 and 25 degrees Celsius)2 Publications
  4. Vmax=2 nmol/min/mg enzyme with DNP-GS as substrate (at pH 8 and 25 degrees Celsius)2 Publications
  5. Vmax=1.8 nmol/min/mg enzyme with DNP-GS as substrate (with 100 µM E217betaG at pH 8 and 25 degrees Celsius)2 Publications
  6. Vmax=3.8 nmol/min/mg enzyme with GSSG as substrate (at pH 8)2 Publications
  7. Vmax=13.6 nmol/min/mg enzyme with MOC-GS as substrate (at pH 8)2 Publications
  8. Vmax=6.3 nmol/min/mg enzyme with Bn-NCC-1 as substrate (at pH 8)2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi649 – 656ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1276 – 1283ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • (+)-abscisic acid D-glucopyranosyl ester transmembrane transport Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G34660-MONOMER.
ReactomeiR-ATH-2142850. Hyaluronan biosynthesis and export.
R-ATH-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.208.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter C family member 2 (EC:3.6.3.44)
Short name:
ABC transporter ABCC.2
Short name:
AtABCC2
Alternative name(s):
ATP-energized glutathione S-conjugate pump 2
Glutathione S-conjugate-transporting ATPase 2
Multidrug resistance-associated protein 2
Gene namesi
Name:ABCC2
Synonyms:EST4, MRP2
Ordered Locus Names:At2g34660
ORF Names:T29F13.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G34660.

Subcellular locationi

  • Vacuole membrane Curated; Multi-pass membrane protein PROSITE-ProRule annotation

  • Note: Tonoplast.1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei37 – 57HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei76 – 96HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei109 – 129HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei145 – 165HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei172 – 192HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei336 – 356HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei440 – 460HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei527 – 547HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei557 – 577HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei914 – 934HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei955 – 975HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1032 – 1054HelicalPROSITE-ProRule annotationAdd BLAST23
Transmembranei1058 – 1077HelicalPROSITE-ProRule annotationAdd BLAST20
Transmembranei1143 – 1163HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1177 – 1197HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plant-type vacuole Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002260731 – 1623ABC transporter C family member 2Add BLAST1623

Proteomic databases

PaxDbiQ42093.
PRIDEiQ42093.

PTM databases

iPTMnetiQ42093.

Expressioni

Tissue specificityi

Ubiquitous, at low levels.2 Publications

Inductioni

By 1-chloro-2,4-dinitrobenzene (CDNB).1 Publication

Gene expression databases

GenevisibleiQ42093. AT.

Interactioni

Subunit structurei

Interacts with FKBP42/TWD1 and probably with calmodulin (CaM).1 Publication

Protein-protein interaction databases

BioGridi3377. 1 interactor.
IntActiQ42093. 1 interactor.
STRINGi3702.AT2G34660.1.

Structurei

3D structure databases

ProteinModelPortaliQ42093.
SMRiQ42093.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini302 – 582ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST281
Domaini614 – 838ABC transporter 1PROSITE-ProRule annotationAdd BLAST225
Domaini921 – 1205ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST285
Domaini1242 – 1476ABC transporter 2PROSITE-ProRule annotationAdd BLAST235

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1236 – 1251Interaction with calmodulin and FKP42/TWD1By similarityAdd BLAST16

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
InParanoidiQ42093.
OMAiYTPVRHE.
OrthoDBiEOG093600AW.
PhylomeDBiQ42093.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFEFIEWYC KPVPNGVWTK QVANAFGAYT PCATDSFVLG ISQLVLLVLC
60 70 80 90 100
LYRIWLALKD HKVERFCLRS RLYNYFLALL AAYATAEPLF RLIMGISVLD
110 120 130 140 150
FDGPGLPPFE AFGLGVKAFA WGAVMVMILM ETKIYIRELR WYVRFAVIYA
160 170 180 190 200
LVGDMVLLNL VLSVKEYYSS YVLYLYTSEV GAQVLFGILL FMHLPNLDTY
210 220 230 240 250
PGYMPVRSET VDDYEYEEIS DGQQICPEKH ANIFDKIFFS WMNPLMTLGS
260 270 280 290 300
KRPLTEKDVW YLDTWDQTET LFTSFQHSWD KELQKPQPWL LRALNNSLGG
310 320 330 340 350
RFWWGGFWKI GNDCSQFVGP LLLNQLLKSM QEDAPAWMGY IYAFSIFVGV
360 370 380 390 400
VFGVLCEAQY FQNVMRVGYR LRSALIAAVF RKSLRLTNEG RRKFQTGKIT
410 420 430 440 450
NLMTTDAESL QQICQSLHTM WSAPFRIIIA LILLYQQLGV ASLIGALLLV
460 470 480 490 500
LMFPLQTVII SKMQKLTKEG LQRTDKRIGL MNEVLAAMDT VKCYAWENSF
510 520 530 540 550
QSKVQTVRDD ELSWFRKSQL LGALNMFILN SIPVLVTIVS FGVFTLLGGD
560 570 580 590 600
LTPARAFTSL SLFAVLRFPL FMLPNIITQV VNANVSLKRL EEVLATEERI
610 620 630 640 650
LLPNPPIEPG EPAISIRNGY FSWDSKGDRP TLSNINLDVP LGSLVAVVGS
660 670 680 690 700
TGEGKTSLIS AILGELPATS DAIVTLRGSV AYVPQVSWIF NATVRDNILF
710 720 730 740 750
GSPFDREKYE RAIDVTSLKH DLELLPGGDL TEIGERGVNI SGGQKQRVSM
760 770 780 790 800
ARAVYSNSDV YIFDDPLSAL DAHVGQQVFE KCIKRELGQK TRVLVTNQLH
810 820 830 840 850
FLSQVDRIVL VHEGTVKEEG TYEELSSNGP LFQRLMENAG KVEEYSEENG
860 870 880 890 900
EAEADQTAEQ PVANGNTNGL QMDGSDDKKS KEGNKKGGKS VLIKQEERET
910 920 930 940 950
GVVSWRVLKR YQDALGGAWV VMMLLLCYVL TEVFRVTSST WLSEWTDAGT
960 970 980 990 1000
PKSHGPLFYN LIYALLSFGQ VLVTLTNSYW LIMSSLYAAK KLHDNMLHSI
1010 1020 1030 1040 1050
LRAPMSFFHT NPLGRIINRF AKDLGDIDRT VAVFVNMFMG QVSQLLSTVV
1060 1070 1080 1090 1100
LIGIVSTLSL WAIMPLLVLF YGAYLYYQNT AREVKRMDSI SRSPVYAQFG
1110 1120 1130 1140 1150
EALNGLSTIR AYKAYDRMAD INGRSMDNNI RFTLVNMGAN RWLGIRLETL
1160 1170 1180 1190 1200
GGLMIWLTAS FAVMQNGRAE NQQAFASTMG LLLSYALNIT SLLTGVLRLA
1210 1220 1230 1240 1250
SLAENSLNAV ERVGNYIEIP PEAPPVIENN RPPPGWPSSG SIKFEDVVLR
1260 1270 1280 1290 1300
YRPQLPPVLH GVSFFIHPTD KVGIVGRTGA GKSSLLNALF RIVEVEKGRI
1310 1320 1330 1340 1350
LIDDCDVGKF GLMDLRKVLG IIPQSPVLFS GTVRFNLDPF GEHNDADLWE
1360 1370 1380 1390 1400
SLERAHLKDT IRRNPLGLDA EVSEAGENFS VGQRQLLSLS RALLRRSKIL
1410 1420 1430 1440 1450
VLDEATAAVD VRTDALIQKT IREEFKSCTM LIIAHRLNTI IDCDKILVLD
1460 1470 1480 1490 1500
SGRVQEFSSP ENLLSNEGSS FSKMVQSTGA ANAEYLRSLV LDNKRAKDDS
1510 1520 1530 1540 1550
HHLQGQRKWL ASSRWAAAAQ FALAASLTSS HNDLQSLEIE DDSSILKRTN
1560 1570 1580 1590 1600
DAVVTLRSVL EGKHDKEIAE SLEEHNISRE GWLSSLYRMV EGLAVMSRLA
1610 1620
RNRMQQPDYN FEGNTFDWDN VEM
Length:1,623
Mass (Da):182,130
Last modified:March 7, 2006 - v2
Checksum:iF074F0F6ED7A4D47
GO

Sequence cautioni

The sequence AAC04246 differs from that shown. Reason: Frameshift at positions 1212 and 1223.Curated
The sequence AAC49798 differs from that shown. Reason: Frameshift at position 1461.Curated
The sequence CAA81250 differs from that shown. Reason: Frameshift at positions 1223 and 1234.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Q → T in AAC04245 (PubMed:9490749).Curated1
Sequence conflicti21Q → T in AAC04246 (PubMed:9490749).Curated1
Sequence conflicti57A → T in AAC49988 (PubMed:9528668).Curated1
Sequence conflicti141W → C in AAC49988 (PubMed:9528668).Curated1
Sequence conflicti193H → R in AAC49988 (PubMed:9528668).Curated1
Sequence conflicti231A → P in AAC04245 (PubMed:9490749).Curated1
Sequence conflicti348V → G in AAC04245 (PubMed:9490749).Curated1
Sequence conflicti348V → G in AAC04246 (PubMed:9490749).Curated1
Sequence conflicti380F → S in AAC49988 (PubMed:9528668).Curated1
Sequence conflicti588K → N in AAC04245 (PubMed:9490749).Curated1
Sequence conflicti835L → V in AAC04246 (PubMed:9490749).Curated1
Sequence conflicti1250R → C in AAC49988 (PubMed:9528668).Curated1
Sequence conflicti1294 – 1295EV → GG in CAA81250 (Ref. 5) Curated2
Sequence conflicti1297K → E in AAC04245 (PubMed:9490749).Curated1
Sequence conflicti1392A → G in AAC49988 (PubMed:9528668).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014960 mRNA. Translation: AAC49988.1.
AF020288 mRNA. Translation: AAC04245.1.
AF020289 Genomic DNA. Translation: AAC04246.1. Frameshift.
AC003096 Genomic DNA. Translation: AAC16268.1.
CP002685 Genomic DNA. Translation: AEC09005.1.
CP002685 Genomic DNA. Translation: AEC09006.1.
Z26467 mRNA. Translation: CAA81250.1. Frameshift.
U96400 mRNA. Translation: AAC49798.1. Frameshift.
PIRiT01369.
RefSeqiNP_001189675.1. NM_001202746.1.
NP_181013.1. NM_129020.3.
UniGeneiAt.19665.

Genome annotation databases

EnsemblPlantsiAT2G34660.1; AT2G34660.1; AT2G34660.
AT2G34660.2; AT2G34660.2; AT2G34660.
GeneIDi818031.
GrameneiAT2G34660.1; AT2G34660.1; AT2G34660.
AT2G34660.2; AT2G34660.2; AT2G34660.
KEGGiath:AT2G34660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF014960 mRNA. Translation: AAC49988.1.
AF020288 mRNA. Translation: AAC04245.1.
AF020289 Genomic DNA. Translation: AAC04246.1. Frameshift.
AC003096 Genomic DNA. Translation: AAC16268.1.
CP002685 Genomic DNA. Translation: AEC09005.1.
CP002685 Genomic DNA. Translation: AEC09006.1.
Z26467 mRNA. Translation: CAA81250.1. Frameshift.
U96400 mRNA. Translation: AAC49798.1. Frameshift.
PIRiT01369.
RefSeqiNP_001189675.1. NM_001202746.1.
NP_181013.1. NM_129020.3.
UniGeneiAt.19665.

3D structure databases

ProteinModelPortaliQ42093.
SMRiQ42093.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3377. 1 interactor.
IntActiQ42093. 1 interactor.
STRINGi3702.AT2G34660.1.

Protein family/group databases

TCDBi3.A.1.208.5. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiQ42093.

Proteomic databases

PaxDbiQ42093.
PRIDEiQ42093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G34660.1; AT2G34660.1; AT2G34660.
AT2G34660.2; AT2G34660.2; AT2G34660.
GeneIDi818031.
GrameneiAT2G34660.1; AT2G34660.1; AT2G34660.
AT2G34660.2; AT2G34660.2; AT2G34660.
KEGGiath:AT2G34660.

Organism-specific databases

TAIRiAT2G34660.

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
InParanoidiQ42093.
OMAiYTPVRHE.
OrthoDBiEOG093600AW.
PhylomeDBiQ42093.

Enzyme and pathway databases

BioCyciARA:AT2G34660-MONOMER.
ReactomeiR-ATH-2142850. Hyaluronan biosynthesis and export.
R-ATH-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiQ42093.

Gene expression databases

GenevisibleiQ42093. AT.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAB2C_ARATH
AccessioniPrimary (citable) accession number: Q42093
Secondary accession number(s): O22449
, O24526, O48907, O48908, O64590
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.