ID   GUN6_ARATH              Reviewed;         620 AA.
AC   Q42059; Q8H160; Q9C7W3;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 2.
DT   16-JUN-2009, entry version 64.
DE   RecName: Full=Endoglucanase 6;
DE            EC=3.2.1.4;
DE   AltName: Full=Endo-1,4-beta glucanase 6;
DE   Flags: Precursor;
GN   OrderedLocusNames=At1g64390; ORFNames=F15H21.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=21016719; PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S.,
RA   White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y.,
RA   Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W.,
RA   Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K.,
RA   Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y.,
RA   Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L.,
RA   Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E.,
RA   Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B.,
RA   Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P.,
RA   Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A.,
RA   Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I.,
RA   Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D.,
RA   Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M.,
RA   Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M.,
RA   Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis
RT   thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   MEDLINE=22954850; PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J.,
RA   Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F.,
RA   Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.,
RA   Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J.,
RA   Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y.,
RA   Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P.,
RA   Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F.,
RA   Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M.,
RA   Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J.,
RA   Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T.,
RA   Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y.,
RA   Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 397-514.
RC   STRAIN=cv. Columbia; TISSUE=Shoot;
RX   MEDLINE=96158348; PubMed=8580968;
RX   DOI=10.1046/j.1365-313X.1996.09010101.x;
RA   Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA   Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA   Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y.,
RA   Marbach J., Fleck J., Clement B., Philipps G., Herve C., Bardet C.,
RA   Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F.,
RA   Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H.,
RA   Hoefte H.;
RT   "Further progress towards a catalogue of all Arabidopsis genes:
RT   analysis of a set of 5000 non-redundant ESTs.";
RL   Plant J. 9:101-124(1996).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=15170254; DOI=10.1007/s00239-003-2571-x;
RA   Libertini E., Li Y., McQueen-Mason S.J.;
RT   "Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene
RT   family.";
RL   J. Mol. Evol. 58:506-515(2004).
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of (1->4)-beta-D-glucosidic
CC       linkages in cellulose, lichenin and cereal beta-D-glucans.
CC   -!- SUBCELLULAR LOCATION: Secreted (By similarity).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E)
CC       family.
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DR   EMBL; AC066689; AAG51703.1; -; Genomic_DNA.
DR   EMBL; AF372940; AAK50080.1; -; mRNA.
DR   EMBL; AY143945; AAN28884.1; -; mRNA.
DR   EMBL; BT000696; AAN31840.1; -; mRNA.
DR   EMBL; Z25957; CAA81116.1; -; mRNA.
DR   IPI; IPI00542601; -.
DR   PIR; A96668; A96668.
DR   RefSeq; NP_176621.1; -.
DR   UniGene; At.17827; -.
DR   UniGene; At.37005; -.
DR   UniGene; At.72023; -.
DR   UniGene; At.72671; -.
DR   HSSP; Q9EYQ2; 1IA6.
DR   CAZy; CBM49; Carbohydrate-Binding Module Family 49.
DR   CAZy; GH9; Glycoside Hydrolase Family 9.
DR   PRIDE; Q42059; -.
DR   GeneID; 842747; -.
DR   GenomeReviews; CT485782_GR; AT1G64390.
DR   KEGG; ath:AT1G64390; -.
DR   NMPDR; fig|3702.1.peg.5855; -.
DR   TAIR; At1g64390; -.
DR   OMA; Q42059; SYANELL.
DR   BRENDA; 3.2.1.4; 302.
DR   GermOnline; AT1G64390; Arabidopsis thaliana.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:EC.
DR   GO; GO:0007047; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR012341; 6hp_glycosidase.
DR   InterPro; IPR019028; CBM_49.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_AS.
DR   Gene3D; G3DSA:1.50.10.10; CelA/Cel48F_cat; 1.
DR   PANTHER; PTHR22298:SF3; Glyco_hydro_9; 1.
DR   Pfam; PF09478; CBM49; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   PROSITE; PS00592; GLYCOSYL_HYDROL_F9_1; 1.
DR   PROSITE; PS00698; GLYCOSYL_HYDROL_F9_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Cell wall biogenesis/degradation;
KW   Cellulose degradation; Complete proteome; Glycoprotein; Glycosidase;
KW   Hydrolase; Polysaccharide degradation; Secreted; Signal.
FT   SIGNAL        1     22       Potential.
FT   CHAIN        23    620       Endoglucanase 6.
FT                                /FTId=PRO_0000249259.
FT   ACT_SITE    411    411       By similarity.
FT   ACT_SITE    463    463       By similarity.
FT   ACT_SITE    472    472       By similarity.
FT   CARBOHYD    554    554       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    564    564       N-linked (GlcNAc...) (Potential).
FT   CONFLICT    196    196       R -> K (in Ref. 2; AAN31840).
FT   CONFLICT    442    442       S -> V (in Ref. 3; CAA81116).
FT   CONFLICT    511    514       MPIR -> NAYS (in Ref. 3; CAA81116).
SQ   SEQUENCE   620 AA;  68592 MW;  57847AE1990D427F CRC64;
     MEKFAPVAAL LLLLLCFPVA FSGHDYGQAL SKSLLFFEAQ RSGVLPRNQR VTWRSHSGLT
     DGKSSGVNLV GGYYDAGDNV KFGLPMAFTV TMMAWSVIEY GNQLQANGEL GNSIDAIKWG
     TDYFIKAHPE PNVLYGEVGD GNTDHYCWQR PEEMTTDRKA YRIDPSNPGS DLAGETAAAM
     AAASIVFRRS NPVYSRLLLT HAYQLFDFAD KYRGKYDSSI TVAQKYYRSV SGYNDELLWA
     AAWLYQASNN QFYLDYLGRN GDAMGGTGWS MTEFGWDVKY AGVQTLVAKF LMQGKAGRHA
     PVFRKYQEKA DSFMCSLLGK SSRNIQKTPG GLIFRQRWNN MQFVTSASFL TTVYSDYLTS
     SRSNLRCAAG NVAPSQLLSF AKSQVDYILG DNPRATSYMV GYGNNFPQRV HHRGSSIVSV
     KVDRTFVTCR GGYATWFSRK GSDPNLLTGA IVGGPDAYDN FADRRDNYEQ TEPATYNNAP
     LLGVLARLSS GHSGYSQFLP VVPAPVVRRP MPIRRPKVTT PVRASGPVAI VQKITSSWVS
     KGRTYYRYST TVINKSSRPL KSLNLSIKNL YGPIWGLSRS GNSFGLPSWM HSLPSGKSLE
     FVYIHSTTPA NVAVSSYTLA
//