Reviewed, UniProtKB/Swiss-Prot Q42059 (GUN6_ARATH)

Names and origin

Protein names

Recommended name:
    Endoglucanase 6
    EC=3.2.1.4
Alternative name(s):
    Endo-1,4-beta glucanase 6

Gene names

Ordered Locus Names:	At1g64390
ORF Names:	F15H21.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	620 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 9 (cellulase E) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cell wall biogenesis/degradation Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbohydrate binding Inferred from electronic annotation. Source: InterPro cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 620	598	Endoglucanase 6		PRO_0000249259
Sites
	Active site	411	1	By similarity
	Active site	463	1	By similarity
	Active site	472	1	By similarity
Amino acid modifications
	Glycosylation	554	1	N-linked (GlcNAc...) Potential
	Glycosylation	564	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	196	1	R → K in AAN31840. Ref.2
	Sequence conflict	442	1	S → V in CAA81116. Ref.3
	Sequence conflict	511 – 514	4	MPIR → NAYS in CAA81116. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q42059-1 [UniParc]. Last modified September 5, 2006. Version 2. Checksum: 57847AE1990D427F	FASTA	620	68,592
	10 20 30 40 50 60 MEKFAPVAAL LLLLLCFPVA FSGHDYGQAL SKSLLFFEAQ RSGVLPRNQR VTWRSHSGLT 70 80 90 100 110 120 DGKSSGVNLV GGYYDAGDNV KFGLPMAFTV TMMAWSVIEY GNQLQANGEL GNSIDAIKWG 130 140 150 160 170 180 TDYFIKAHPE PNVLYGEVGD GNTDHYCWQR PEEMTTDRKA YRIDPSNPGS DLAGETAAAM 190 200 210 220 230 240 AAASIVFRRS NPVYSRLLLT HAYQLFDFAD KYRGKYDSSI TVAQKYYRSV SGYNDELLWA 250 260 270 280 290 300 AAWLYQASNN QFYLDYLGRN GDAMGGTGWS MTEFGWDVKY AGVQTLVAKF LMQGKAGRHA 310 320 330 340 350 360 PVFRKYQEKA DSFMCSLLGK SSRNIQKTPG GLIFRQRWNN MQFVTSASFL TTVYSDYLTS 370 380 390 400 410 420 SRSNLRCAAG NVAPSQLLSF AKSQVDYILG DNPRATSYMV GYGNNFPQRV HHRGSSIVSV 430 440 450 460 470 480 KVDRTFVTCR GGYATWFSRK GSDPNLLTGA IVGGPDAYDN FADRRDNYEQ TEPATYNNAP 490 500 510 520 530 540 LLGVLARLSS GHSGYSQFLP VVPAPVVRRP MPIRRPKVTT PVRASGPVAI VQKITSSWVS 550 560 570 580 590 600 KGRTYYRYST TVINKSSRPL KSLNLSIKNL YGPIWGLSRS GNSFGLPSWM HSLPSGKSLE 610 620 FVYIHSTTPA NVAVSSYTLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Further progress towards a catalogue of all Arabidopsis genes: analysis of a set of 5000 non-redundant ESTs." Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C., Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R., Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J. , Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D., Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P., Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H. Plant J. 9:101-124(1996) [PubMed: 8580968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 397-514. Strain: cv. Columbia. Tissue: Shoot.
[4]	"Phylogenetic analysis of the plant endo-beta-1,4-glucanase gene family." Libertini E., Li Y., McQueen-Mason S.J. J. Mol. Evol. 58:506-515(2004) [PubMed: 15170254] [Abstract] Cited for: GENE FAMILY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC066689 Genomic DNA. Translation: AAG51703.1. AF372940 mRNA. Translation: AAK50080.1. AY143945 mRNA. Translation: AAN28884.1. BT000696 mRNA. Translation: AAN31840.1. Z25957 mRNA. Translation: CAA81116.1.
IPI	IPI00542601.
PIR	A96668.
RefSeq	NP_176621.1.
UniGene	At.17827 At.37005 At.72023 At.72671
3D structure databases
HSSP	HSSP built from PDB template 1IA6 based on UniProtKB Q9EYQ2.
ModBase	Search...
Protein family/group databases
CAZy	CBM49. Carbohydrate-Binding Module Family 49. GH9. Glycoside Hydrolase Family 9.
Proteomic databases
PRIDE	Q42059.
Genome annotation databases
GeneID	842747.
GenomeReviews	Gene locus AT1G64390 in contig CT485782_GR.
KEGG	ath:AT1G64390.
NMPDR	fig|3702.1.peg.5855.
Organism-specific databases
TAIR	At1g64390.
Phylogenomic databases
OMA	Q42059. SYANELL.
Enzyme and pathway databases
BRENDA	3.2.1.4. 302.
Gene expression databases
GermOnline	AT1G64390. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR012341. 6hp_glycosidase. IPR019028. CBM_49. IPR001701. Glyco_hydro_9. IPR018221. Glyco_hydro_9_AS. [Graphical view]
Gene3D	G3DSA:1.50.10.10. CelA/Cel48F_cat. 1 hit.
PANTHER	PTHR22298:SF3. Glyco_hydro_9. 1 hit.
Pfam	PF09478. CBM49. 1 hit. PF00759. Glyco_hydro_9. 1 hit. [Graphical view]
PROSITE	PS00592. GLYCOSYL_HYDROL_F9_1. 1 hit. PS00698. GLYCOSYL_HYDROL_F9_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN6_ARATH

Accession

Primary (citable) accession number: Q42059
Secondary accession number(s): Q8H160, Q9C7W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	September 5, 2006
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)