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Q41969

- IF2B_ARATH

UniProt

Q41969 - IF2B_ARATH

Protein

Eukaryotic translation initiation factor 2 subunit beta

Gene

EMB1401

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (10 Jan 2003)
      Previous versions | rss
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    Functioni

    eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri222 – 24625C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: TAIR
    3. translation initiation factor activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Initiation factor

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Eukaryotic translation initiation factor 2 subunit beta
    Short name:
    eIF-2-beta
    Alternative name(s):
    Protein EMBRYO DEFECTIVE 1401
    Gene namesi
    Name:EMB1401
    Ordered Locus Names:At5g20920
    ORF Names:F22D1.90
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G20920.

    Subcellular locationi

    GO - Cellular componenti

    1. plasma membrane Source: TAIR

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 268267Eukaryotic translation initiation factor 2 subunit betaPRO_0000137411Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiQ41969.
    PRIDEiQ41969.

    Expressioni

    Gene expression databases

    GenevestigatoriQ41969.

    Interactioni

    Subunit structurei

    Heterotrimer composed of an alpha, a beta and a gamma chain.

    Protein-protein interaction databases

    BioGridi17491. 25 interactions.
    IntActiQ41969. 32 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ41969.
    SMRiQ41969. Positions 116-252.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi22 – 287Poly-Lys
    Compositional biasi64 – 696Poly-Lys

    Sequence similaritiesi

    Belongs to the eIF-2-beta/eIF-5 family.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri222 – 24625C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1601.
    HOGENOMiHOG000107197.
    InParanoidiQ41969.
    KOiK03238.
    OMAiWEGSERD.
    PhylomeDBiQ41969.

    Family and domain databases

    Gene3Di3.30.30.50. 1 hit.
    InterProiIPR002735. Transl_init_fac_IF2/IF5.
    IPR016189. Transl_init_fac_IF2/IF5_N.
    IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
    [Graphical view]
    PfamiPF01873. eIF-5_eIF-2B. 1 hit.
    [Graphical view]
    SMARTiSM00653. eIF2B_5. 1 hit.
    [Graphical view]
    SUPFAMiSSF100966. SSF100966. 1 hit.
    SSF75689. SSF75689. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: Q41969-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADEINEIRE EQEQLAPFDP SKKKKKKKVV IQEPVEDLAE SSQTEKSDSL    50
    PVNDGLESSF TGMKKKKKKP TESSLLNNES VDAGEDLDEI ANDEQEGEEG 100
    IVLQQRYPWE GSERDYIYDE LLGRVFNILR ENNPELAGDR RRTVMRPPQV 150
    LREGTKKTVF VNFMDLCKTM HRQPDHVMQY LLAELGTSGS LDGQQRLVVK 200
    GRFAPKNFEG ILRRYITDYV ICLGCKSPDT ILSKENRLFF LRCEKCGSQR 250
    SVAPIKTGFV ARVSRRKT 268
    Length:268
    Mass (Da):30,663
    Last modified:January 10, 2003 - v3
    Checksum:iE1C17373472F4DEB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti71 – 711T → A in AAK29672. 1 PublicationCurated
    Sequence conflicti71 – 711T → A in AAM65346. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353095 mRNA. Translation: AAK29672.1.
    AF296834 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92904.1.
    CP002688 Genomic DNA. Translation: AED92906.1.
    AY128324 mRNA. Translation: AAM91527.1.
    BT000056 mRNA. Translation: AAN15375.1.
    AY087810 mRNA. Translation: AAM65346.1.
    Z18133 mRNA. Translation: CAA79110.1.
    RefSeqiNP_001078610.1. NM_001085141.1. [Q41969-1]
    NP_197592.1. NM_122100.3. [Q41969-1]
    UniGeneiAt.21465.

    Genome annotation databases

    EnsemblPlantsiAT5G20920.1; AT5G20920.1; AT5G20920. [Q41969-1]
    AT5G20920.3; AT5G20920.3; AT5G20920. [Q41969-1]
    GeneIDi832216.
    KEGGiath:AT5G20920.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF353095 mRNA. Translation: AAK29672.1 .
    AF296834 Genomic DNA. No translation available.
    CP002688 Genomic DNA. Translation: AED92904.1 .
    CP002688 Genomic DNA. Translation: AED92906.1 .
    AY128324 mRNA. Translation: AAM91527.1 .
    BT000056 mRNA. Translation: AAN15375.1 .
    AY087810 mRNA. Translation: AAM65346.1 .
    Z18133 mRNA. Translation: CAA79110.1 .
    RefSeqi NP_001078610.1. NM_001085141.1. [Q41969-1 ]
    NP_197592.1. NM_122100.3. [Q41969-1 ]
    UniGenei At.21465.

    3D structure databases

    ProteinModelPortali Q41969.
    SMRi Q41969. Positions 116-252.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 17491. 25 interactions.
    IntActi Q41969. 32 interactions.

    Proteomic databases

    PaxDbi Q41969.
    PRIDEi Q41969.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G20920.1 ; AT5G20920.1 ; AT5G20920 . [Q41969-1 ]
    AT5G20920.3 ; AT5G20920.3 ; AT5G20920 . [Q41969-1 ]
    GeneIDi 832216.
    KEGGi ath:AT5G20920.

    Organism-specific databases

    TAIRi AT5G20920.

    Phylogenomic databases

    eggNOGi COG1601.
    HOGENOMi HOG000107197.
    InParanoidi Q41969.
    KOi K03238.
    OMAi WEGSERD.
    PhylomeDBi Q41969.

    Miscellaneous databases

    PROi Q41969.

    Gene expression databases

    Genevestigatori Q41969.

    Family and domain databases

    Gene3Di 3.30.30.50. 1 hit.
    InterProi IPR002735. Transl_init_fac_IF2/IF5.
    IPR016189. Transl_init_fac_IF2/IF5_N.
    IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
    [Graphical view ]
    Pfami PF01873. eIF-5_eIF-2B. 1 hit.
    [Graphical view ]
    SMARTi SM00653. eIF2B_5. 1 hit.
    [Graphical view ]
    SUPFAMi SSF100966. SSF100966. 1 hit.
    SSF75689. SSF75689. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Browning K.S., Chen R.
      Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-252.
      Strain: cv. Columbia.
      Tissue: Seedling.
    7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
      Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
      Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiIF2B_ARATH
    AccessioniPrimary (citable) accession number: Q41969
    Secondary accession number(s): Q8L7Q3, Q9C5N7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Translation initiation factors
      List of translation initiation factor entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3