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Protein

Eukaryotic translation initiation factor 2 subunit beta

Gene

EMB1401

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri222 – 24625C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. translation initiation factor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_302829. Ribosomal scanning and start codon recognition.
REACT_310327. Recycling of eIF2:GDP.
REACT_310661. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_325381. Formation of the ternary complex, and subsequently, the 43S complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit beta
Short name:
eIF-2-beta
Alternative name(s):
Protein EMBRYO DEFECTIVE 1401
Gene namesi
Name:EMB1401
Ordered Locus Names:At5g20920
ORF Names:F22D1.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20920.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 268267Eukaryotic translation initiation factor 2 subunit betaPRO_0000137411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ41969.
PRIDEiQ41969.

Expressioni

Gene expression databases

GenevestigatoriQ41969.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Protein-protein interaction databases

BioGridi17491. 25 interactions.
IntActiQ41969. 32 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ41969.
SMRiQ41969. Positions 116-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi22 – 287Poly-Lys
Compositional biasi64 – 696Poly-Lys

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri222 – 24625C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1601.
HOGENOMiHOG000107197.
InParanoidiQ41969.
KOiK03238.
OMAiIQDPADE.
PhylomeDBiQ41969.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q41969-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEINEIRE EQEQLAPFDP SKKKKKKKVV IQEPVEDLAE SSQTEKSDSL
60 70 80 90 100
PVNDGLESSF TGMKKKKKKP TESSLLNNES VDAGEDLDEI ANDEQEGEEG
110 120 130 140 150
IVLQQRYPWE GSERDYIYDE LLGRVFNILR ENNPELAGDR RRTVMRPPQV
160 170 180 190 200
LREGTKKTVF VNFMDLCKTM HRQPDHVMQY LLAELGTSGS LDGQQRLVVK
210 220 230 240 250
GRFAPKNFEG ILRRYITDYV ICLGCKSPDT ILSKENRLFF LRCEKCGSQR
260
SVAPIKTGFV ARVSRRKT
Length:268
Mass (Da):30,663
Last modified:January 10, 2003 - v3
Checksum:iE1C17373472F4DEB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti71 – 711T → A in AAK29672 (Ref. 1) Curated
Sequence conflicti71 – 711T → A in AAM65346 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353095 mRNA. Translation: AAK29672.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92904.1.
CP002688 Genomic DNA. Translation: AED92906.1.
AY128324 mRNA. Translation: AAM91527.1.
BT000056 mRNA. Translation: AAN15375.1.
AY087810 mRNA. Translation: AAM65346.1.
Z18133 mRNA. Translation: CAA79110.1.
RefSeqiNP_001078610.1. NM_001085141.1. [Q41969-1]
NP_197592.1. NM_122100.3. [Q41969-1]
UniGeneiAt.21465.

Genome annotation databases

EnsemblPlantsiAT5G20920.1; AT5G20920.1; AT5G20920. [Q41969-1]
AT5G20920.3; AT5G20920.3; AT5G20920. [Q41969-1]
GeneIDi832216.
KEGGiath:AT5G20920.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353095 mRNA. Translation: AAK29672.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92904.1.
CP002688 Genomic DNA. Translation: AED92906.1.
AY128324 mRNA. Translation: AAM91527.1.
BT000056 mRNA. Translation: AAN15375.1.
AY087810 mRNA. Translation: AAM65346.1.
Z18133 mRNA. Translation: CAA79110.1.
RefSeqiNP_001078610.1. NM_001085141.1. [Q41969-1]
NP_197592.1. NM_122100.3. [Q41969-1]
UniGeneiAt.21465.

3D structure databases

ProteinModelPortaliQ41969.
SMRiQ41969. Positions 116-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17491. 25 interactions.
IntActiQ41969. 32 interactions.

Proteomic databases

PaxDbiQ41969.
PRIDEiQ41969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20920.1; AT5G20920.1; AT5G20920. [Q41969-1]
AT5G20920.3; AT5G20920.3; AT5G20920. [Q41969-1]
GeneIDi832216.
KEGGiath:AT5G20920.

Organism-specific databases

TAIRiAT5G20920.

Phylogenomic databases

eggNOGiCOG1601.
HOGENOMiHOG000107197.
InParanoidiQ41969.
KOiK03238.
OMAiIQDPADE.
PhylomeDBiQ41969.

Enzyme and pathway databases

ReactomeiREACT_302829. Ribosomal scanning and start codon recognition.
REACT_310327. Recycling of eIF2:GDP.
REACT_310661. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_325381. Formation of the ternary complex, and subsequently, the 43S complex.

Miscellaneous databases

PROiQ41969.

Gene expression databases

GenevestigatoriQ41969.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Browning K.S., Chen R.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-252.
    Strain: cv. Columbia.
    Tissue: Seedling.
  7. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiIF2B_ARATH
AccessioniPrimary (citable) accession number: Q41969
Secondary accession number(s): Q8L7Q3, Q9C5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 10, 2003
Last modified: April 1, 2015
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.