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Protein

Eukaryotic translation initiation factor 2 subunit beta

Gene

EIF2B

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri222 – 246C4-typeSequence analysisAdd BLAST25

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.
R-ATH-72731. Recycling of eIF2:GDP.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit betaCurated
Short name:
eIF2-betaCurated
Alternative name(s):
Protein EMBRYO DEFECTIVE 1401Curated
Gene namesi
Name:EIF2BCurated
Synonyms:EMB1401Curated
Ordered Locus Names:At5g20920
ORF Names:F22D1.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20920.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: TAIR
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001374112 – 268Eukaryotic translation initiation factor 2 subunit betaAdd BLAST267

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei42Phosphoserine; by CK21 Publication1
Modified residuei80Phosphoserine; by CK21 Publication1
Modified residuei112Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylated at Ser-42, Ser-80 and Ser-112 by CK2.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ41969.
PRIDEiQ41969.

PTM databases

iPTMnetiQ41969.

Expressioni

Gene expression databases

ExpressionAtlasiQ41969. baseline and differential.
GenevisibleiQ41969. AT.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain.

Protein-protein interaction databases

BioGridi17491. 32 interactors.
IntActiQ41969. 32 interactors.
STRINGi3702.AT5G20920.1.

Structurei

3D structure databases

ProteinModelPortaliQ41969.
SMRiQ41969.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi22 – 28Poly-Lys7
Compositional biasi64 – 69Poly-Lys6

Sequence similaritiesi

Belongs to the eIF-2-beta/eIF-5 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri222 – 246C4-typeSequence analysisAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2768. Eukaryota.
COG1601. LUCA.
HOGENOMiHOG000107197.
InParanoidiQ41969.
KOiK03238.
OMAiQNELVAD.
OrthoDBiEOG09360J8U.
PhylomeDBiQ41969.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q41969-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADEINEIRE EQEQLAPFDP SKKKKKKKVV IQEPVEDLAE SSQTEKSDSL
60 70 80 90 100
PVNDGLESSF TGMKKKKKKP TESSLLNNES VDAGEDLDEI ANDEQEGEEG
110 120 130 140 150
IVLQQRYPWE GSERDYIYDE LLGRVFNILR ENNPELAGDR RRTVMRPPQV
160 170 180 190 200
LREGTKKTVF VNFMDLCKTM HRQPDHVMQY LLAELGTSGS LDGQQRLVVK
210 220 230 240 250
GRFAPKNFEG ILRRYITDYV ICLGCKSPDT ILSKENRLFF LRCEKCGSQR
260
SVAPIKTGFV ARVSRRKT
Length:268
Mass (Da):30,663
Last modified:January 10, 2003 - v3
Checksum:iE1C17373472F4DEB
GO
Isoform 2 (identifier: Q41969-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     14-14: Missing.

Note: May be due to a competing acceptor splice site. No experimental confirmation available.Curated
Show »
Length:267
Mass (Da):30,535
Checksum:i5EB1B92B370F7321
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71T → A in AAK29672 (Ref. 1) Curated1
Sequence conflicti71T → A in AAM65346 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05849014Missing in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353095 mRNA. Translation: AAK29672.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92904.1.
CP002688 Genomic DNA. Translation: AED92905.1.
CP002688 Genomic DNA. Translation: AED92906.1.
AY128324 mRNA. Translation: AAM91527.1.
BT000056 mRNA. Translation: AAN15375.1.
BX829594 mRNA. No translation available.
AY087810 mRNA. Translation: AAM65346.1.
Z18133 mRNA. Translation: CAA79110.1.
RefSeqiNP_001078610.1. NM_001085141.1. [Q41969-1]
NP_197592.1. NM_122100.4. [Q41969-1]
NP_974817.1. NM_203088.2. [Q41969-2]
UniGeneiAt.21465.

Genome annotation databases

EnsemblPlantsiAT5G20920.1; AT5G20920.1; AT5G20920. [Q41969-1]
AT5G20920.2; AT5G20920.2; AT5G20920. [Q41969-2]
AT5G20920.3; AT5G20920.3; AT5G20920. [Q41969-1]
GeneIDi832216.
GrameneiAT5G20920.1; AT5G20920.1; AT5G20920.
AT5G20920.2; AT5G20920.2; AT5G20920.
AT5G20920.3; AT5G20920.3; AT5G20920.
KEGGiath:AT5G20920.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF353095 mRNA. Translation: AAK29672.1.
AF296834 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92904.1.
CP002688 Genomic DNA. Translation: AED92905.1.
CP002688 Genomic DNA. Translation: AED92906.1.
AY128324 mRNA. Translation: AAM91527.1.
BT000056 mRNA. Translation: AAN15375.1.
BX829594 mRNA. No translation available.
AY087810 mRNA. Translation: AAM65346.1.
Z18133 mRNA. Translation: CAA79110.1.
RefSeqiNP_001078610.1. NM_001085141.1. [Q41969-1]
NP_197592.1. NM_122100.4. [Q41969-1]
NP_974817.1. NM_203088.2. [Q41969-2]
UniGeneiAt.21465.

3D structure databases

ProteinModelPortaliQ41969.
SMRiQ41969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17491. 32 interactors.
IntActiQ41969. 32 interactors.
STRINGi3702.AT5G20920.1.

PTM databases

iPTMnetiQ41969.

Proteomic databases

PaxDbiQ41969.
PRIDEiQ41969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20920.1; AT5G20920.1; AT5G20920. [Q41969-1]
AT5G20920.2; AT5G20920.2; AT5G20920. [Q41969-2]
AT5G20920.3; AT5G20920.3; AT5G20920. [Q41969-1]
GeneIDi832216.
GrameneiAT5G20920.1; AT5G20920.1; AT5G20920.
AT5G20920.2; AT5G20920.2; AT5G20920.
AT5G20920.3; AT5G20920.3; AT5G20920.
KEGGiath:AT5G20920.

Organism-specific databases

TAIRiAT5G20920.

Phylogenomic databases

eggNOGiKOG2768. Eukaryota.
COG1601. LUCA.
HOGENOMiHOG000107197.
InParanoidiQ41969.
KOiK03238.
OMAiQNELVAD.
OrthoDBiEOG09360J8U.
PhylomeDBiQ41969.

Enzyme and pathway databases

ReactomeiR-ATH-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-ATH-72702. Ribosomal scanning and start codon recognition.
R-ATH-72731. Recycling of eIF2:GDP.

Miscellaneous databases

PROiQ41969.

Gene expression databases

ExpressionAtlasiQ41969. baseline and differential.
GenevisibleiQ41969. AT.

Family and domain databases

Gene3Di3.30.30.50. 1 hit.
InterProiIPR002735. Transl_init_fac_IF2/IF5.
IPR016189. Transl_init_fac_IF2/IF5_N.
IPR016190. Transl_init_fac_IF2/IF5_Zn-bd.
[Graphical view]
PfamiPF01873. eIF-5_eIF-2B. 1 hit.
[Graphical view]
SMARTiSM00653. eIF2B_5. 1 hit.
[Graphical view]
SUPFAMiSSF100966. SSF100966. 1 hit.
SSF75689. SSF75689. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B_ARATH
AccessioniPrimary (citable) accession number: Q41969
Secondary accession number(s): F4K6W6, Q8L7Q3, Q9C5N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 10, 2003
Last modified: November 30, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.