Q41969 (IF2B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 2 subunit beta
Short name=eIF-2-beta

Alternative name(s):
Protein EMBRYO DEFECTIVE 1401

Gene names

Name:	EMB1401
Ordered Locus Names:	At5g20920
ORF Names:	F22D1.90

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B By similarity.
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain.
Sequence similarities	Belongs to the eIF-2-beta/eIF-5 family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Initiation factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q41969-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Eukaryotic translation initiation factor 2 subunit beta		PRO_0000137411
Regions
	Zinc finger	222 – 246	25	C4-type Potential
	Compositional bias	22 – 28	7	Poly-Lys
	Compositional bias	64 – 69	6	Poly-Lys
Experimental info
	Sequence conflict	71	1	T → A in AAK29672. Ref.1
	Sequence conflict	71	1	T → A in AAM65346. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 10, 2003. Version 3. Checksum: E1C17373472F4DEB	FASTA	268	30,663
	10 20 30 40 50 60 MADEINEIRE EQEQLAPFDP SKKKKKKKVV IQEPVEDLAE SSQTEKSDSL PVNDGLESSF 70 80 90 100 110 120 TGMKKKKKKP TESSLLNNES VDAGEDLDEI ANDEQEGEEG IVLQQRYPWE GSERDYIYDE 130 140 150 160 170 180 LLGRVFNILR ENNPELAGDR RRTVMRPPQV LREGTKKTVF VNFMDLCKTM HRQPDHVMQY 190 200 210 220 230 240 LLAELGTSGS LDGQQRLVVK GRFAPKNFEG ILRRYITDYV ICLGCKSPDT ILSKENRLFF 250 260 LRCEKCGSQR SVAPIKTGFV ARVSRRKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Browning K.S., Chen R. Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B. Plant J. 4:1051-1061(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 97-252. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF353095 mRNA. Translation: AAK29672.1. AF296834 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED92904.1. CP002688 Genomic DNA. Translation: AED92906.1. AY128324 mRNA. Translation: AAM91527.1. BT000056 mRNA. Translation: AAN15375.1. AY087810 mRNA. Translation: AAM65346.1. Z18133 mRNA. Translation: CAA79110.1.
IPI	IPI00516553.
RefSeq	NP_001078610.1. NM_001085141.1. NP_197592.1. NM_122100.3.
UniGene	At.21465.
3D structure databases
ProteinModelPortal	Q41969.
SMR	Q41969. Positions 116-252.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q41969. 26 interactions.
Proteomic databases
PaxDb	Q41969.
PRIDE	Q41969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G20920.1; AT5G20920.1; AT5G20920. AT5G20920.3; AT5G20920.3; AT5G20920.
GeneID	832216.
KEGG	ath:AT5G20920.
Organism-specific databases
TAIR	At5g20920.
Phylogenomic databases
eggNOG	COG1601.
HOGENOM	HOG000107197.
InParanoid	Q41969.
KO	K03238.
OMA	DKENQEP.
PhylomeDB	Q41969.
ProtClustDB	CLSN2687406.
Gene expression databases
ArrayExpress	Q41969.
Genevestigator	Q41969.
Family and domain databases
Gene3D	3.30.30.50. 1 hit.
InterPro	IPR002735. Transl_init_fac_IF2/IF5. IPR016189. Transl_init_fac_IF2/IF5_N. IPR016190. Transl_init_fac_IF2/IF5_Zn-bd. [Graphical view]
Pfam	PF01873. eIF-5_eIF-2B. 1 hit. [Graphical view]
SMART	SM00653. eIF2B_5. 1 hit. [Graphical view]
SUPFAM	SSF100966. Transl_init_fac_IF2/IF5_N. 1 hit. SSF75689. Transl_init_fac_IF2/IF5_Zn-bd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

IF2B_ARATH

Accession

Primary (citable) accession number: Q41969
Secondary accession number(s): Q8L7Q3, Q9C5N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 10, 2003
Last modified:	May 1, 2013
This is version 106 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program