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Protein

1-aminocyclopropane-1-carboxylate oxidase 2

Gene

ACO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Enzyme involved in the ethylene biosynthesis. Required to mediate the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination via endosperm rupture. May promote stem elongation by maximizing the extensibility cells, possibly by activating ethylene biosynthesis, in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0).2 Publications

Catalytic activityi

1-aminocyclopropane-1-carboxylate + ascorbate + O2 = ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O.

Cofactori

Fe2+PROSITE-ProRule annotation1 Publication, Cu2+PROSITE-ProRule annotation1 PublicationNote: Binds 1 Fe2+ ion per subunit. Can also bind Cu2+ ions.PROSITE-ProRule annotation1 Publication

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 2 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 8 (ACS8), 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4), 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 7 (ACS7), 1-aminocyclopropane-1-carboxylate synthase 11 (ACS11), 1-aminocyclopropane-1-carboxylate synthase 9 (ACS9), 1-aminocyclopropane-1-carboxylate synthase 6 (ACS6), 1-aminocyclopropane-1-carboxylate synthase 5 (ACS5)
  2. 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 3 (At1g12010), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4), 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 5 (At1g77330)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi180 – 1801IronPROSITE-ProRule annotation
Metal bindingi182 – 1821IronPROSITE-ProRule annotation
Metal bindingi237 – 2371IronPROSITE-ProRule annotation
Binding sitei247 – 24712-oxoglutaratePROSITE-ProRule annotation

GO - Molecular functioni

  • 1-aminocyclopropane-1-carboxylate oxidase activity Source: TAIR
  • copper ion binding Source: TAIR
  • L-ascorbic acid binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to fatty acid Source: UniProtKB
  • cellular response to iron ion Source: TAIR
  • cellular response to nitric oxide Source: UniProtKB
  • defense response Source: UniProtKB-KW
  • detection of ethylene stimulus Source: TAIR
  • ethylene biosynthetic process Source: TAIR
  • positive regulation of seed germination Source: UniProtKB
  • response to cytokinin Source: TAIR
  • response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ethylene biosynthesis, Plant defense

Keywords - Ligandi

Copper, Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

UniPathwayiUPA00384; UER00563.

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate oxidase 2 (EC:1.14.17.4)
Short name:
ACC oxidase 2
Short name:
AtACO2
Gene namesi
Name:ACO2
Synonyms:EI305
Ordered Locus Names:At1g62380
ORF Names:F24O1.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G62380.

Subcellular locationi

GO - Cellular componenti

  • cell wall Source: TAIR
  • cytosol Source: TAIR
  • endoplasmic reticulum Source: TAIR
  • Golgi apparatus Source: TAIR
  • plasma membrane Source: TAIR
  • plasmodesma Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Impaired in the 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated reversion of the ABA-induced inhibition of seed germination.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3203201-aminocyclopropane-1-carboxylate oxidase 2PRO_0000408298Add
BLAST

Proteomic databases

PaxDbiQ41931.
PRIDEiQ41931.

PTM databases

iPTMnetiQ41931.
SwissPalmiQ41931.

Expressioni

Tissue specificityi

Expressed in vegetative tissues. Constitutively expressed in leaves and blades. In ethylene exposed etiolated seedlings, localized in cells at the outer side of the exaggerated hook in an ethylene-dependent manner and following an ethylene sensitive pattern. Also detected in the root tip when treated by ethylene.3 Publications

Inductioni

Upon iron deprivation. Induced by ethylene, particularly in root tips and hooks of ethiolated seedlings. Promoted by ozone O3. Accumulates in response to very-long-chain fatty acids (VLCFAs C20:0 to C30:0). Induced in roots by nitric oxide (NO).7 Publications

Gene expression databases

GenevisibleiQ41931. AT.

Interactioni

Protein-protein interaction databases

BioGridi27757. 1 interaction.
IntActiQ41931. 1 interaction.
STRINGi3702.AT1G62380.1.

Structurei

3D structure databases

ProteinModelPortaliQ41931.
SMRiQ41931. Positions 7-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini156 – 256101Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili111 – 14333Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
KOiK05933.
OMAiLACKETE.
PhylomeDBiQ41931.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q41931-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKNMKFPVV DLSKLNGEER DQTMALINEA CENWGFFEIV NHGLPHDLMD
60 70 80 90 100
KIEKMTKDHY KTCQEQKFND MLKSKGLDNL ETEVEDVDWE STFYVRHLPQ
110 120 130 140 150
SNLNDISDVS DEYRTAMKDF GKRLENLAED LLDLLCENLG LEKGYLKKVF
160 170 180 190 200
HGTKGPTFGT KVSNYPPCPK PEMIKGLRAH TDAGGIILLF QDDKVSGLQL
210 220 230 240 250
LKDGDWIDVP PLNHSIVINL GDQLEVITNG KYKSVLHRVV TQQEGNRMSV
260 270 280 290 300
ASFYNPGSDA EISPATSLVE KDSEYPSFVF DDYMKLYAGV KFQPKEPRFA
310 320
AMKNASAVTE LNPTAAVETF
Length:320
Mass (Da):36,183
Last modified:November 1, 1998 - v2
Checksum:iE9B22DF21FDE35A4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti261 – 2611E → D in AAC27484 (PubMed:10409511).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016100 mRNA. Translation: AAC27484.1.
AC003113 Genomic DNA. Translation: AAF70838.1.
CP002684 Genomic DNA. Translation: AEE33960.1.
AY045876 mRNA. Translation: AAK76550.1.
AY062685 mRNA. Translation: AAL32763.1.
AY093381 mRNA. Translation: AAM13380.1.
AY133851 mRNA. Translation: AAM91785.1.
AK230351 mRNA. Translation: BAF02150.1.
Z17775 mRNA. Translation: CAA79062.1.
PIRiT01448.
T52267.
RefSeqiNP_176428.1. NM_104918.4.
UniGeneiAt.24621.
At.37653.

Genome annotation databases

EnsemblPlantsiAT1G62380.1; AT1G62380.1; AT1G62380.
GeneIDi842536.
GrameneiAT1G62380.1; AT1G62380.1; AT1G62380.
KEGGiath:AT1G62380.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016100 mRNA. Translation: AAC27484.1.
AC003113 Genomic DNA. Translation: AAF70838.1.
CP002684 Genomic DNA. Translation: AEE33960.1.
AY045876 mRNA. Translation: AAK76550.1.
AY062685 mRNA. Translation: AAL32763.1.
AY093381 mRNA. Translation: AAM13380.1.
AY133851 mRNA. Translation: AAM91785.1.
AK230351 mRNA. Translation: BAF02150.1.
Z17775 mRNA. Translation: CAA79062.1.
PIRiT01448.
T52267.
RefSeqiNP_176428.1. NM_104918.4.
UniGeneiAt.24621.
At.37653.

3D structure databases

ProteinModelPortaliQ41931.
SMRiQ41931. Positions 7-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27757. 1 interaction.
IntActiQ41931. 1 interaction.
STRINGi3702.AT1G62380.1.

PTM databases

iPTMnetiQ41931.
SwissPalmiQ41931.

Proteomic databases

PaxDbiQ41931.
PRIDEiQ41931.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G62380.1; AT1G62380.1; AT1G62380.
GeneIDi842536.
GrameneiAT1G62380.1; AT1G62380.1; AT1G62380.
KEGGiath:AT1G62380.

Organism-specific databases

TAIRiAT1G62380.

Phylogenomic databases

eggNOGiKOG0143. Eukaryota.
COG3491. LUCA.
HOGENOMiHOG000276735.
KOiK05933.
OMAiLACKETE.
PhylomeDBiQ41931.

Enzyme and pathway databases

UniPathwayiUPA00384; UER00563.

Miscellaneous databases

PROiQ41931.

Gene expression databases

GenevisibleiQ41931. AT.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEiPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of differential growth in the apical hook of Arabidopsis."
    Raz V., Ecker J.R.
    Development 126:3661-3668(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY ETHYLENE.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The Arabidopsis thaliana transcribed genome: the GDR cDNA program."
    Raynal M., Grellet F., Laudie M., Meyer Y., Cooke R., Delseny M.
    Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 230-320.
    Strain: cv. Columbia.
    Tissue: Green siliques.
  7. "Ethylene and auxin control the Arabidopsis response to decreased light intensity."
    Vandenbussche F., Vriezen W.H., Smalle J., Laarhoven L.J.J., Harren F.J.M., Van Der Straeten D.
    Plant Physiol. 133:517-527(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Transcriptional profiling by cDNA-AFLP and microarray analysis reveals novel insights into the early response to ethylene in Arabidopsis."
    De Paepe A., Vuylsteke M., Van Hummelen P., Zabeau M., Van Der Straeten D.
    Plant J. 39:537-559(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ETHYLENE.
    Strain: cv. Columbia.
  9. "Ozone-induced programmed cell death in the Arabidopsis radical-induced cell death1 mutant."
    Overmyer K., Brosche M., Pellinen R., Kuittinen T., Tuominen H., Ahlfors R., Keinaenen M., Saarma M., Scheel D., Kangasjaervi J.
    Plant Physiol. 137:1092-1104(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY OZONE.
    Strain: cv. Columbia.
  10. "ETHYLENE-INSENSITIVE5 encodes a 5'-->3' exoribonuclease required for regulation of the EIN3-targeting F-box proteins EBF1/2."
    Olmedo G., Guo H., Gregory B.D., Nourizadeh S.D., Aguilar-Henonin L., Li H., An F., Guzman P., Ecker J.R.
    Proc. Natl. Acad. Sci. U.S.A. 103:13286-13293(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY ETHYLENE.
    Strain: cv. Columbia.
  11. "Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis."
    Qin Y.-M., Hu C.-Y., Pang Y., Kastaniotis A.J., Hiltunen J.K., Zhu Y.-X.
    Plant Cell 19:3692-3704(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION BY VERY-LONG-CHAIN FATTY ACIDS, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  12. "Ethylene interacts with abscisic acid to regulate endosperm rupture during germination: a comparative approach using Lepidium sativum and Arabidopsis thaliana."
    Linkies A., Mueller K., Morris K., Tureckova V., Wenk M., Cadman C.S.C., Corbineau F., Strnad M., Lynn J.R., Finch-Savage W.E., Leubner-Metzger G.
    Plant Cell 21:3803-3822(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  13. "Ethylene and nitric oxide involvement in the up-regulation of key genes related to iron acquisition and homeostasis in Arabidopsis."
    Garcia M.J., Lucena C., Romera F.J., Alcantara E., Perez-Vicente R.
    J. Exp. Bot. 61:3885-3899(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY IRON DEFICIENCY.
  14. "Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function."
    Tan Y.-F., O'Toole N., Taylor N.L., Millar A.H.
    Plant Physiol. 152:747-761(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR.
  15. "A new model involving ethylene, nitric oxide and Fe to explain the regulation of Fe-acquisition genes in Strategy I plants."
    Garcia M.J., Suarez V., Romera F.J., Alcantara E., Perez-Vicente R.
    Plant Physiol. Biochem. 49:537-544(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY NITRIC OXIDE.

Entry informationi

Entry nameiACCO2_ARATH
AccessioniPrimary (citable) accession number: Q41931
Secondary accession number(s): O81093, Q7DLM8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 1, 1998
Last modified: February 17, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.