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Protein

4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 2, chloroplastic

Gene

GLU2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Beta-glucosidase acting poorly on artificial aryl beta-glucosides. Has no activity toward the chromogenic substrate 6-bromo-2-naphthyl-beta-D-glucoside (6BNGlc).1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.1 Publication
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose.1 Publication
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose.1 Publication

Kineticsi

  1. KM=0.84 mM for p-nitrophenyl beta-D-glucopyranoside (PNPG) (with recombinant enzyme)1 Publication
  2. KM=1.25 mM for o-nitrophenyl beta-D-glucopyranoside (with recombinant enzyme)1 Publication
  1. Vmax=52.8 µmol/h/µg enzyme with p-nitrophenyl beta-D-glucopyranoside as substrate (with recombinant enzyme)1 Publication
  2. Vmax=168.3 µmol/h/µg enzyme with o-nitrophenyl beta-D-glucopyranoside as substrate (with recombinant enzyme)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891SubstrateBy similarity
Binding sitei193 – 1931SubstrateBy similarity
Binding sitei241 – 2411SubstrateBy similarity
Active sitei242 – 2421Proton donorBy similarity
Binding sitei384 – 3841SubstrateBy similarity
Active sitei457 – 4571NucleophilePROSITE-ProRule annotation
Binding sitei508 – 5081SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 2, chloroplastic (EC:3.2.1.182)
Alternative name(s):
Beta-glucosidase 2 (EC:3.2.1.21)
Short name:
ZmGlu2
Gene namesi
Name:GLU2
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151ChloroplastSequence analysisAdd
BLAST
Chaini52 – 5635124-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase 2, chloroplasticPRO_0000424097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi261 ↔ 267By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ41761.
PRIDEiQ41761.

Expressioni

Tissue specificityi

Expressed in leaves only starting at day 6 after germination.1 Publication

Interactioni

Subunit structurei

Homo- and heterodimer.1 Publication

Protein-protein interaction databases

STRINGi4577.GRMZM2G008247_P01.

Structurei

3D structure databases

ProteinModelPortaliQ41761.
SMRiQ41761. Positions 67-558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni322 – 35837DimerizationBy similarityAdd
BLAST
Regioni391 – 40212DimerizationBy similarityAdd
BLAST
Regioni447 – 4504DimerizationBy similarity
Regioni515 – 5162Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41761-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPLLAAAMN HAAHPVLRSH LGPNNESFSR HHLSSSPQSS KRRFNLSFTP
60 70 80 90 100
RSARVGNQNG VQLLSPSEIP RRDWFPSDFI FGAATSAYQI EGAWNEDGKG
110 120 130 140 150
ESNWDHFCHN FPERIMDGSN ADIGANSYHM YKTDVRLLKE MGMDAYRFSI
160 170 180 190 200
SWPRILPKGT VEGGINQDGI DYYKRLINLL LENGIEPYVT IFHWDVPQAL
210 220 230 240 250
EEKYGGFLDK TQKRIVNDYK NFAKVCFDNF GDKVKNWLTF NEPQTFTSFS
260 270 280 290 300
YGTGVFAPGR CSPGLDCAIP TGNSLVEPYI AGHNILLAHA EAVDLYNKYY
310 320 330 340 350
KGENGRIGLA FDVMGRVPYG TSFLDEQAKE RSMDINLGWF LEPVVRGDYP
360 370 380 390 400
FSMRSLARER LPFFSDKQQE KLVGSYNMLG INYYTSIFSK HIDISPKYSP
410 420 430 440 450
VLNTDDAYAS QETYGPDGKP IGPPMGNPWI YLYPEGLKDI LMIMKNKYGN
460 470 480 490 500
PPIYITENGI GDVDTKEKPL PMEAALNDYK RLDYIQRHIS TLKESIDLGA
510 520 530 540 550
NVHGYFAWSL LDNFEWYAGY TERYGIVYVD RKNNYTRYMK ESAKWLKEFN
560
TAKKPSKKII TPA
Length:563
Mass (Da):64,111
Last modified:November 1, 1996 - v1
Checksum:iD8FD8E31215E2A9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44087 mRNA. Translation: AAD09850.1.
PIRiT02720.
RefSeqiNP_001105892.1. NM_001112422.1.
UniGeneiZm.160665.

Genome annotation databases

GeneIDi732807.
KEGGizma:732807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U44087 mRNA. Translation: AAD09850.1.
PIRiT02720.
RefSeqiNP_001105892.1. NM_001112422.1.
UniGeneiZm.160665.

3D structure databases

ProteinModelPortaliQ41761.
SMRiQ41761. Positions 67-558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G008247_P01.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PaxDbiQ41761.
PRIDEiQ41761.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi732807.
KEGGizma:732807.

Phylogenomic databases

eggNOGiKOG0626. Eukaryota.
COG2723. LUCA.
HOGENOMiHOG000088630.
KOiK01188.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHGGL2_MAIZE
AccessioniPrimary (citable) accession number: Q41761
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.