Reviewed, UniProtKB/Swiss-Prot Q41745 (LPAT_MAIZE)

Names and origin

Protein names

Recommended name:
    1-acyl-sn-glycerol-3-phosphate acyltransferase PLS1
    EC=2.3.1.51
Alternative name(s):
    Phospholipid synthesis protein 1

Gene names

Name:

PLS1

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate. Ref.1
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Probable.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	1-acyl-sn-glycerol-3-phosphate acyltransferase PLS1		PRO_0000208188
Regions
	Transmembrane	15 – 37	23	Potential
	Transmembrane	48 – 68	21	Potential
	Transmembrane	121 – 141	21	Potential
	Transmembrane	305 – 325	21	Potential
	Transmembrane	335 – 355	21	Potential
	Motif	92 – 97	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q41745-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F1F5492CAFF24F93	FASTA	374	42,571
	10 20 30 40 50 60 MAIPLVLVVL PLGLLFLLSG LIVNAIQAVL FVTIRPFSKS FYRRINRFLA ELLWLQLVWV 70 80 90 100 110 120 VDWWAGVKVQ LHADEETYRS MGKEHALIIS NHRSDIDWLI GWILAQRSGC LGSTLAVMKK 130 140 150 160 170 180 SSKFLPVIGW SMWFAEYLFL ERSWAKDEKT LKWGLQRLKD FPRPFWLALF VEGTRFTPAK 190 200 210 220 230 240 LLAAQEYAAS QGLPAPRNVL IPRTKGFVSA VSIMRDFVPA IYDTTVIVPK DSPQPTMLRI 250 260 270 280 290 300 LKGQSSVIHV RMKRHAMSEM PKSDEDVSKW CKDIFVAKDA LLDKHLATGT FDEEIRPIGR 310 320 330 340 350 360 PVKSLLVTLF WSCLLLFGAI EFFKWTQLLS TWRGVAFTAA GMALVTGVMH VFIMFSQAER 370 SSSARAARNR VKKE

References

[1]

"Isolation and characterization of a maize cDNA that complements a 1-acyl sn-glycerol-3-phosphate acyltransferase mutant of E.coli and encodes a protein which has similarities to other acyltransferases." Brown A.P., Coleman J., Tommey A.M., Watson M.D., Slabas A.R. Plant Mol. Biol. 26:211-223(1994) [PubMed: 7948871] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ENZYME ACTIVITY. Strain: cv. Black Mexican Sweet. Tissue: Endosperm.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z29518 mRNA. Translation: CAA82638.1.
PIR	S52645.
RefSeq	NP_001105919.1.
UniGene	Zm.95920
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	732844.
Organism-specific databases
Gramene	Q41745.
MaizeGDB	98837.
Enzyme and pathway databases
BRENDA	2.3.1.51. 289.
Family and domain databases
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPAT_MAIZE

Accession

Primary (citable) accession number: Q41745

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	November 1, 1996
Last modified:	March 3, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)