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Q41638 (XTHA_PHAAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xyloglucan endotransglucosylase/hydrolase protein A

EC=2.4.1.207
Alternative name(s):
VaXTH1
Gene names
Name:XTHA
Synonyms:EXT, XTH1
OrganismPhaseolus angularis (Azuki bean) (Vigna angularis)
Taxonomic identifier3914 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. Ref.3

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Tissue specificity

Predominantly expressed in the phloem fibers of growing internodes. Expressed in xylem cells in the basal part of the internode. In the internode, it is expressed closer to the top of the internode compared to XTHB. Ref.2

Induction

Up-regulated by indole-3-acetic acid (IAA) and fusicoccin. Effect of IAA is however nullified in 0.25 M mannitol, which prevents cell expansion without affecting auxin action per se. Ref.2

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.1
Chain21 – 292272Xyloglucan endotransglucosylase/hydrolase protein A
PRO_0000011837

Sites

Active site1051Nucleophile By similarity
Active site1091Proton donor By similarity

Amino acid modifications

Glycosylation1131N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q41638 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: AFA721A5C3B440AA

FASTA29233,882
        10         20         30         40         50         60 
MGSSLWTCLI LLSLASASFA ANPRTPIDVP FGRNYVPTWA FDHIKYLNGG SEIQLHLDKY 

        70         80         90        100        110        120 
TGTGFQSKGS YLFGHFSMYI KLVPGDSAGT VTAFYLSSTN AEHDEIDFEF LGNRTGQPYI 

       130        140        150        160        170        180 
LQTNVFTGGK GDREQRIYLW FDPTTQYHRY SVLWNMYQIV FYVDDYPIRV FKNSNDLGVK 

       190        200        210        220        230        240 
FPFNQPMKIY NSLWNADDWA TRGGLEKTDW SKAPFIASYK GFHIDGCEAS VNAKFCDTQG 

       250        260        270        280        290 
KRWWDQPEFR DLDAAQWQKL AWVRNKYTIY NYCTDRKRYS QVPPECTRDR DI 

« Hide

References

[1]"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-50.
Strain: cv. Takara Wase.
[2]"Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin."
Nakamura T., Yokoyama R., Tomita E., Nishitani K.
Plant Cell Physiol. 44:16-24(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
Strain: cv. Takara Wase.
[3]"Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule."
Nishitani K., Tominaga R.
J. Biol. Chem. 267:21058-21064(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, ENZYME ACTIVITY.
Strain: cv. Takara Wase.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16458 mRNA. Translation: BAA03925.1.
AB086395 Genomic DNA. Translation: BAC03237.1.
PIRA49539.

3D structure databases

ProteinModelPortalQ41638.
SMRQ41638. Positions 26-292.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTHA_PHAAN
AccessionPrimary (citable) accession number: Q41638
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries