Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q41638

- XTHA_PHAAN

UniProt

Q41638 - XTHA_PHAAN

Protein

Xyloglucan endotransglucosylase/hydrolase protein A

Gene

XTHA

Organism
Phaseolus angularis (Azuki bean) (Vigna angularis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei105 – 1051NucleophilePROSITE-ProRule annotation
    Active sitei109 – 1091Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
    2. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xyloglucan endotransglucosylase/hydrolase protein A (EC:2.4.1.207)
    Alternative name(s):
    VaXTH1
    Gene namesi
    Name:XTHA
    Synonyms:EXT, XTH1
    OrganismiPhaseolus angularis (Azuki bean) (Vigna angularis)
    Taxonomic identifieri3914 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 20201 PublicationAdd
    BLAST
    Chaini21 – 292272Xyloglucan endotransglucosylase/hydrolase protein APRO_0000011837Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi113 – 1131N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Predominantly expressed in the phloem fibers of growing internodes. Expressed in xylem cells in the basal part of the internode. In the internode, it is expressed closer to the top of the internode compared to XTHB.1 Publication

    Inductioni

    Up-regulated by indole-3-acetic acid (IAA) and fusicoccin. Effect of IAA is however nullified in 0.25 M mannitol, which prevents cell expansion without affecting auxin action per se.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ41638.
    SMRiQ41638. Positions 26-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q41638-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSSLWTCLI LLSLASASFA ANPRTPIDVP FGRNYVPTWA FDHIKYLNGG    50
    SEIQLHLDKY TGTGFQSKGS YLFGHFSMYI KLVPGDSAGT VTAFYLSSTN 100
    AEHDEIDFEF LGNRTGQPYI LQTNVFTGGK GDREQRIYLW FDPTTQYHRY 150
    SVLWNMYQIV FYVDDYPIRV FKNSNDLGVK FPFNQPMKIY NSLWNADDWA 200
    TRGGLEKTDW SKAPFIASYK GFHIDGCEAS VNAKFCDTQG KRWWDQPEFR 250
    DLDAAQWQKL AWVRNKYTIY NYCTDRKRYS QVPPECTRDR DI 292
    Length:292
    Mass (Da):33,882
    Last modified:November 1, 1996 - v1
    Checksum:iAFA721A5C3B440AA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16458 mRNA. Translation: BAA03925.1.
    AB086395 Genomic DNA. Translation: BAC03237.1.
    PIRiA49539.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D16458 mRNA. Translation: BAA03925.1 .
    AB086395 Genomic DNA. Translation: BAC03237.1 .
    PIRi A49539.

    3D structure databases

    ProteinModelPortali Q41638.
    SMRi Q41638. Positions 26-292.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR008263. Glycoside_hydrolase_16_AS.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS01034. GLYCOSYL_HYDROL_F16. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
      Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
      J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-50.
      Strain: cv. Takara Wase.
    2. "Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin."
      Nakamura T., Yokoyama R., Tomita E., Nishitani K.
      Plant Cell Physiol. 44:16-24(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
      Strain: cv. Takara Wase.
    3. "Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule."
      Nishitani K., Tominaga R.
      J. Biol. Chem. 267:21058-21064(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, ENZYME ACTIVITY.
      Strain: cv. Takara Wase.

    Entry informationi

    Entry nameiXTHA_PHAAN
    AccessioniPrimary (citable) accession number: Q41638
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3