Q41638 (XTHA_PHAAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Xyloglucan endotransglucosylase/hydrolase protein A
EC=2.4.1.207

Alternative name(s):
VaXTH1

Gene names

Name:	XTHA
Synonyms:	EXT, XTH1

Organism

Phaseolus angularis (Azuki bean) (Vigna angularis)

Taxonomic identifier

3914 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.
Catalytic activity	Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan. Ref.3
Subcellular location	Secreted › cell wall Probable. Secreted › extracellular space › apoplast Probable.
Tissue specificity	Predominantly expressed in the phloem fibers of growing internodes. Expressed in xylem cells in the basal part of the internode. In the internode, it is expressed closer to the top of the internode compared to XTHB. Ref.2
Induction	Up-regulated by indole-3-acetic acid (IAA) and fusicoccin. Effect of IAA is however nullified in 0.25 M mannitol, which prevents cell expansion without affecting auxin action per se. Ref.2
Post-translational modification	Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Apoplast Cell wall Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellular glucan metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	apoplast Inferred from electronic annotation. Source: UniProtKB-SubCell cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro xyloglucan:xyloglucosyl transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.1
	Chain	21 – 292	272	Xyloglucan endotransglucosylase/hydrolase protein A		PRO_0000011837
Sites
	Active site	105	1	Nucleophile By similarity
	Active site	109	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	113	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q41638 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: AFA721A5C3B440AA	FASTA	292	33,882
	10 20 30 40 50 60 MGSSLWTCLI LLSLASASFA ANPRTPIDVP FGRNYVPTWA FDHIKYLNGG SEIQLHLDKY 70 80 90 100 110 120 TGTGFQSKGS YLFGHFSMYI KLVPGDSAGT VTAFYLSSTN AEHDEIDFEF LGNRTGQPYI 130 140 150 160 170 180 LQTNVFTGGK GDREQRIYLW FDPTTQYHRY SVLWNMYQIV FYVDDYPIRV FKNSNDLGVK 190 200 210 220 230 240 FPFNQPMKIY NSLWNADDWA TRGGLEKTDW SKAPFIASYK GFHIDGCEAS VNAKFCDTQG 250 260 270 280 290 KRWWDQPEFR DLDAAQWQKL AWVRNKYTIY NYCTDRKRYS QVPPECTRDR DI

References

[1]	"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls." Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K. J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-50. Strain: cv. Takara Wase.
[2]	"Two azuki bean XTH genes, VaXTH1 and VaXTH2, with similar tissue-specific expression profiles, are differently regulated by auxin." Nakamura T., Yokoyama R., Tomita E., Nishitani K. Plant Cell Physiol. 44:16-24(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION. Strain: cv. Takara Wase.
[3]	"Endo-xyloglucan transferase, a novel class of glycosyltransferase that catalyzes transfer of a segment of xyloglucan molecule to another xyloglucan molecule." Nishitani K., Tominaga R. J. Biol. Chem. 267:21058-21064(1992) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, ENZYME ACTIVITY. Strain: cv. Takara Wase.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D16458 mRNA. Translation: BAA03925.1. AB086395 Genomic DNA. Translation: BAC03237.1.
PIR	A49539.
3D structure databases
ProteinModelPortal	Q41638.
SMR	Q41638. Positions 26-292.
ModBase	Search...
Protein family/group databases
CAZy	GH16. Glycoside Hydrolase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR000757. Glyco_hydro_16. IPR008263. Glycoside_hydrolase_16_AS. IPR016455. XET. IPR010713. XET_C. [Graphical view]
Pfam	PF00722. Glyco_hydro_16. 1 hit. PF06955. XET_C. 1 hit. [Graphical view]
PIRSF	PIRSF005604. XET. 1 hit.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XTHA_PHAAN

Accession

Primary (citable) accession number: Q41638

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program