Reviewed, UniProtKB/Swiss-Prot Q41596 (CHI1_THECC)

Names and origin

Protein names

Recommended name:
    Endochitinase 1
    EC=3.2.1.14

Gene names

Name:

CHIA1

Organism

Theobroma cacao (Cacao) (Cocoa)

Taxonomic identifier

3641 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Malvales › Malvaceae › Byttnerioideae › Theobroma

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Vacuole By similarity. Note: Vacuolar and protoplast By similarity.
Induction	In response to infection, elicitor, ethylene, wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Cellular component	Vacuole
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 321	300	Endochitinase 1		PRO_0000005329
Regions
	Domain	22 – 64	43	Chitin-binding type-1
	Region	65 – 98	34	Hinge
	Region	99 – 321	223	Catalytic
Amino acid modifications
	Disulfide bond	24 ↔ 39		By similarity
	Disulfide bond	33 ↔ 45		By similarity
	Disulfide bond	38 ↔ 52		By similarity
	Disulfide bond	58 ↔ 62		By similarity
	Disulfide bond	93 ↔ 156		By similarity
	Disulfide bond	167 ↔ 175		By similarity
	Disulfide bond	274 ↔ 306		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q41596-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E87DBFBC8D7A2ADB	FASTA	321	34,848
	10 20 30 40 50 60 MSFRALSVFS LFLSYLILGS AEQCGRQAGG ALCPGGLCCS QFGWCGNTDD YCKKENGCQS 70 80 90 100 110 120 QCSGSGGDTG GLDSLITRER FDQMLLHRND GGCPARGFYT YDAFIAAAKS FPAFATTGDD 130 140 150 160 170 180 ATRKREVAAF LAQTSHETTG GAGWAAPDGP YTWGYCYNRE LNPADYCQWD PNYPCAPGKQ 190 200 210 220 230 240 YFGRGPMQLT WNYNYGQCGR AIGVDLLNNP DLLATDPTIS FKSAFWFWMT PQSPKPSCHD 250 260 270 280 290 300 VIIGAWSPSG SDQAAGRVPG FGLITNIING GLECGQGWNA KVEDRIGFYK RYCDTLGVGY 310 320 GNNLDCYNQR SYNNGPSVDS M

References

[1]	"A genomic clone from Theobroma cacao L. with high similarity to plant class I endochitinase sequences." Snyder-Leiby T.E., Furtek D.B. Plant Gene Register PGR95-056 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U30324 Genomic DNA. Translation: AAA80656.1.
3D structure databases
HSSP	HSSP built from PDB template 1HEV based on UniProtKB P02877.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 18928.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHI1_THECC

Accession

Primary (citable) accession number: Q41596

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)